Peter Szeremy

• Doctor of Philosophy
• Research Fellow at Obuda University

Background: Current clinical guidelines support family-centered care in Neonatal Intensive Care Units (NICUs). This implies parents should also be involved in the most critical patient safety measures. Hand hygiene is the single most important tool to prevent healthcare-associated infections and related long-term effects. Although often studied in...

Healthcare-Associated Infections present a major threat to patient safety globally. According to studies, more than 50% of HAI could be prevented by proper hand hygiene. Effectiveness of hand hygiene is regularly evaluated with the fluorescent method: performing hand hygiene with a handrub containing an ultra violet (UV) fluorescent marker. Typical...

Recent studies put under scrutiny the prevailing hand hygiene guidelines, which incorporate quantitative parameters regarding handrub volume and hand size. Understanding the criticality of complete (i.e., efficient) hand hygiene in healthcare, objectivization of hand hygiene related parameters are paramount, including the formulation of the ABHR. S...

Background Recent studies put under scrutiny the prevailing hand hygiene guidelines, which incorporate quantitative parameters regarding handrub volume and hand size. Understanding the criticality of complete (i.e., efficient) hand hygiene in healthcare, objectivization of hand hygiene related parameters are paramount, including the formulation of...

Background Healthcare-associated infections represent a major burden in neonatal intensive care units. Hand antisepsis is the most important tool for prevention, however, compliance among healthcare workers remains low. Objectives To prospectively evaluate the influence of different work shifts (extended working hours, night shifts) on the quality...

Background Multidrug resistance transporters (MDR-ABC transporters, namely MDR1, MRP1 and BCRP) play essential role in the homeostasis of a variety of endogenous molecules, proinflammatory mediators among them [1]. When the regulation of this phenomenon is disturbed, cell migration, proliferation and inflammatory processes are over activated which...

Objective Multidrug resistance (MDR) transporters may be used as biomarkers to monitor disease progression in RA and as a predictive tool to establish responsiveness to biological therapy. In this multicenter clinical trial, we aimed to assess the predictive value of MDR1, MRP1 and BCRP activity measurement for biological therapeutic response in RA...

Background MDR transporters are important biomarkers of drug resistance in cancer and in autoimmune conditions. We determined the MDR1, MRP1 and BCRP activity in CD3+ lymphocytes using a flow cytometry based method from 120 healthy volunteers in order to describe normal reference values of the activity of these transporters. The effects of gender a...

Ultraviolet spectrum markers are widely used for hand hygiene quality assessment, although their microbiological validation has not been established. A microbiology-based assessment of the procedure was conducted. Twenty-five artificial hand models underwent initial full contamination, then disinfection with UV-dyed hand rub solution, digital imagi...

Background Multi-Drug Resistance (MDR) protein function is an independent prognostic marker in certain haematological malignancies. Immune activation is also linked to the expression of MDR proteins in autoimmune disorders. Furthermore, MDR protein function may predict patient response to traditional DMARD, as well as biological treatment helping t...

Background: ABCB1 is a key ABC efflux transporter modulating the pharmacokinetics of a large percentage of drugs. ABCB1 is also a site of transporter mediated drug-drug interactions (tDDI). It is the transporter most frequently tested for tDDIs both in vitro and in the clinic. Objective: Understanding the limitations of various in vitro and in v...

The multidrug-resistant phenotype of tumor cells is acquired via an increased capability of drug efflux by ABC transporters and causes serious problems in cancer treatment. With the aim to uncover whether changes induced by epigenetic mechanisms in the expression level of drug transporter genes correlates with changes in the drug resistance phenoty...

MDR-ABC transporters are widely expressed in cell types relevant to pathogenesis of rheumatoid arthritis. Many reports demonstrate the interaction of small molecule drugs with MDR-ABC transporters. Cell-based assays for disease relevant cell types can be easily gated and could reveal specific drug targets and may increase significance and utilisati...

The multidrug resistance protein 1 (MDR1) transporter is the most abundantly investigated adenosine triphosphate (ATP)-Binding Cassette (ABC) transporter protein. Multiple assay systems were developed to study MDR1-mediated transport and possible drug-drug interactions. Yet, as different probe substrates are used in these assays, it is difficult to...

Aim: To develop a set of assays, utilizing CalceinAM as common probe substrate for the investigation of P-gp mediated drug-drug interactions. Methods: A dye efflux assay, utilizing P-gp overexpressing human cells and CalceinAM, a substrate of the transporter was used to study the inhibitory properties of the selected compound set Two membrane based...