Peter Sseruwagi

Peter Sseruwagi
Mikocheni Agricultural Research Institute (MARI) · Department of Biotechnology

PhD, Entomology/Plant Health Management and IPM

About

221
Publications
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Introduction
Dr. Peter Sseruwagi is an Entomologist, with a PhD from the University of the Witwatersrand, South Africa, he obtained in 2005. He is currently working at Mikocheni Agricultural Research Institute, Tanzania, as the Entomologist and Technical Assistant Coordinator for the ‘Disease diagnostics for sustainable cassava productivity in Africa’ project, which aims to build human and infrastructural capacities of African NARS in east, central and southern Africa His main research goal is to understand and control insect pests and vectors of crop viruses in SSA. He has 26 years’ research experience in: invasive insect biology, ecology, vector-virus-plant interactions, molecular biology, pest and disease crop resistance devt, population dynamics, survey methodology. He has 42 SI scientific papers.

Publications

Publications (221)
Article
Full-text available
Over the past three decades, highly increased whitefly (Bemisia tabaci) populations have been observed on the staple food crop cassava in eastern Africa and associated with ensuing viral disease pandemics and food insecurity. Increased whitefly numbers have also been observed in other key agricultural crops and weeds. Factors behind the population...
Article
Full-text available
In East Africa, the prevalent Bemisia tabaci whiteflies on the food security crop cassava are classified as sub-Saharan Africa (SSA) species. Economically damaging cassava whitefly populations were associated with the SSA2 species in the 1990s, but more recently, it has been to SSA1 species. To investigate whether biological traits (number of first...
Article
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Context African production landscapes are diverse, with multiple cassava cultivars grown in small patches amongst a diversity of other crops. Studies on how diverse smallholder landscapes impact herbivore pest outbreak risk have not been carried out in sub-Saharan Africa. Objectives Bemisia tabaci is a cryptic pest species complex that cause damag...
Article
Full-text available
Bemisia tabaci is an important vector of cassava brown streak viruses and cassava mosaic begomoviruses, the causal agents of cassava brown streak disease and cassava mosaic disease (CMD), respectively. A study was carried out to determine the genetic variability of B. tabaci associated with cassava and the occurrence of CMD in Zambia in 2013 and 20...
Article
Full-text available
In sub-Saharan Africa cassava growing areas, two members of the Bemisia tabaci species complex termed sub-Saharan Africa 1 (SSA1) and SSA2 have been reported as the prevalent whiteflies associated with the spread of viruses that cause cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) pandemics. At the peak of CMD pandemic in the...
Article
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Background: The whitefly Bemisia tabaci is an important vector of virus diseases, impacting cassava production in East Africa. To date, breeding efforts in this region have focused on disease resistance. Here we use a spatially-explicit simulation model to explore how breeding strategies for whitefly resistance will influence the population dynami...
Article
Full-text available
Cassava is a staple food crop in sub-Saharan Africa; it is a rich source of carbohydrates and proteins which currently supports livelihoods of more than 800 million people worldwide. However, its continued production is at stake due to vector-transmitted diseases such as Cassava mosaic disease and Cassava brown streak disease. Currently, the manage...
Article
Full-text available
Cassava brown streak disease (CBSD) is currently the most devastating cassava disease in eastern, central and southern Africa affecting a staple crop for over 700 million people on the continent. A major outbreak of CBSD in 2004 near Kampala rapidly spread across Uganda. In the following years, similar CBSD outbreaks were noted in countries across...
Article
Begomoviruses are plant viruses that cause major losses to many economically important crops. Although they are poorly understood, begomoviruses infecting wild plants may have an important role as reservoirs in the epidemiology of viral diseases. This study reports the discovery and genomic characterization of three novel bipartite begomoviruses fr...
Article
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In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with the MinION and MinIT mobile sequencing devices developed by Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The PDQeX extracts DNA using a cocktail of thermophilic proteinases and cell...
Preprint
Full-text available
In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with the MinION and MinIT mobile sequencing devices developed by Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The PDQeX extracts DNA using a cocktail of thermophilic proteinases and cell...
Article
Full-text available
Whitefly (Bemisia tabaci), a major pest and vector of viruses in cassava, is the greatest current threat to cassava production in sub-Saharan Africa (SSA). Research efforts have focused on management of the two viral diseases: cassava mosaic disease (CMD) and cassava brown streak disease (CBSD), and have ignored the whitefly vector that is driving...
Chapter
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The Cassava Diagnostics Project (CDP) project (2008–2018) was implemented in Zambia through the Zambia Agricultural Research Institute (ZARI). In the 10 years of its implementation, several objectives set in three main aims were successfully implemented and their milestones accomplished. Four countrywide surveys were conducted in seven provinces. D...
Article
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Cassava (Manihot esculenta Crantz) is one of the most important root staple crops in Zambia. An estimated 30% of Zambians, over 4 million people, consume cassava as part of their daily diet. Cassava is mostly grown by subsistence farmers on fields of less than 1 ha. Cultivation of cassava is hampered by several biotic constraints, of which cassava...
Article
Full-text available
Cassava (Manihot esculenta Crantz) is one of the most important root staple crops in Zambia. An estimated 30% of Zambians, over 4 million people, consume cassava as part of their daily diet. Cassava is mostly grown by subsistence farmers on fields of less than 1 ha. Cultivation of cassava is hampered by several biotic constraints, of which cassava...
Preprint
Over the last 20 years the authors have been involved in collaborative research projects in Africa, where we have gained valuable experience and knowledge in effective collaboration in agricultural development and research for impact. We have effectively collaborated with more than 100 number of institutions globally, regionally, and nationally on...
Article
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Cassava brown streak disease (CBSD) caused by Cassava brown streak virus (CBSV) and Uganda cassava brown streak virus (UCBSV) is a major constraint to cassava production in Mozambique. Full genome sequences of CBSD‐associated virus isolates contribute to the understanding of genetic diversity and the development of new diagnostic primers that can b...
Book
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A number of stakeholders benefited from the implementation of the CDP – there is now a strong connection with the end-users of the science and technology. Farmers who used to get less than 5 t/ha of cassava before the project intervention are now getting 20–45 t/ha after receiving virus-free improved cassava material from the project. This has sign...
Article
A study was conducted to determine the fecundity (number of eggs laid) and survivorship of two sub-Saharan Africa 1 (SSA1) populations of Bemisia tabaci: subgroup 1 (SSA1-SG1) obtained from the Lake Victoria Basin, and subgroup 3 (SSA1-SG3) from Coast Region, on healthy and cassava mosaic begomovirus (CMB)-infected cassava plants in Tanzania in Apr...
Article
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Cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) are two viral diseases that cause severe yield losses in cassava of up to 100%, thereby persistently threatening food and income security in sub-Saharan Africa. For effective management of these diseases, there is a critical need to develop and deploy varieties with dual resistanc...
Article
Full-text available
Cassava brown streak disease (CBSD) caused by Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) is the main constraint to cassava (Manihot esculenta Crantz) production in Mozambique. Using RT-PCR to amplify partial coat protein nucleotide sequences, we detected for the first time the occurrence of CBSV in two non-cass...
Article
Emerging plant viruses are one of the greatest problems facing crop production worldwide, and have severe consequences in the developing world where subsistence farming is a major source of food production, and knowledge and resources for management are limited. In Africa, evolution of two viral disease complexes, cassava mosaic begomoviruses (CMBs...
Article
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Passion fruit production has decreased over years in Uganda. Biotic and management related factors have been implicated as the cause, with viruses taking lead. In order to understand the production environment of Passion fruit, this study was initiated. Specifically, the focus was to investigate the relationship between farmers’ knowledge of virus...
Book
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The 3rd International Whitefly Symposium (IWS) is a merger with the International Bemisia Workshop (IBWS) and the European Whitefly Symposium (EWS) in 2013. The 1st IWS merged Symposium (IWS1) was held 20 – 23 May 2013 on Crete, Greece and the 2nd IWS was held in Arusha, Tanzania from 14-19 February 2016. The symposium was held in Perth from 16 – 1...
Article
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Crop losses due to viral diseases and pests are major constraints on food security and income for millions of households in sub-Saharan Africa (SSA). Such losses can be reduced if plant diseases and pests are correctly diagnosed and identified early. Currently, accurate diagnosis for definitive identification of plant viruses and their vectors in S...
Article
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The localization of Cassava brown streak virus (CBSV) in cassava (Manihot esculenta) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed prec...
Preprint
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The United Nations has listed Zero Hunger as one of the 17 global sustainable development goals to end extreme poverty by 2030. Plant viruses are a major constraint to crop production globally causing an estimated $30 billion in damage leaving millions of people food insecure. In Africa, agriculture employs up to 50% of the workforce, yet only cont...
Article
Full-text available
Background: Bemisia tabaci species (B. tabaci), or whiteflies, are the world's most devastating insect pests. They cause billions of dollars (US) of damage each year, and are leaving farmers in the developing world food insecure. Currently, all publically available transcriptome data forB. tabaciare generated from pooled samples, which can lead to...
Article
Full-text available
Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a pest species complex that causes widespread damage to cassava, a staple food crop for millions of households in East Africa. Species in the complex cause direct feeding damage to cassava and are the vectors of multiple plant viruses. Whilst significant work has gone into developing virus-resi...
Article
Full-text available
Background: Bemisia tabaci species ( B. tabaci ), or whiteflies, are the world’s most devastating insect pests. They cause billions of dollars (US) of damage each year, and are leaving farmers in the developing world food insecure. Currently, all publically available transcriptome data for B. tabaci are generated from pooled samples, which can lead...
Article
Full-text available
Bemisia tabaci whitefly species are some of the world's most devastating agricultural pests and plant-virus disease vectors. Elucidation of the phylogenetic relationships in the group is the basis for understanding their evolution, biogeography, gene-functions and development of novel control technologies. We report here the discovery of five new S...
Article
Full-text available
Background:Bemisia tabaci species ( B. tabaci ), or whiteflies, are the world’s most devastating insect pests. They cause billions of dollars (US) of damage each year, and are leaving farmers in the developing world food insecure. Currently, all publically available transcriptome data for B. tabaci are generated from pooled samples, which can lead...
Article
Full-text available
Cassava is the main staple food for over 800 million people globally. Its production in eastern Africa is being constrained by two devastating Ipomoviruses that cause cassava brown streak disease (CBSD); Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), with up to 100% yield loss for smallholder farmers in the region...
Data
111 cassava brown streak virus sequences downloaded from GenBank all with the highly conserved DAG motif in the coat protein. (PDF)
Article
Full-text available
Cassava Brown Streak Disease (CBSD) continues to spread and its effect on productivity remains at high level losses (50-100 %) in Sub-Saharan Africa. However, there is little knowledge about the drivers of adoption on CBSD control measures in Rwanda. Thus, this study investigated the drivers to adopt CBSD control measures in Rwanda during 2015-2016...
Article
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Weed-infecting begomoviruses play an important role in the epidemiology of crop diseases because they can potentially infect crops and contribute to the genetic diversity of crop-infecting begomoviruses. Despite the important epidemiological role that weed-infecting begomoviruses play, they remain insufficiently studied in Africa. Recently, we iden...
Poster
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The leaf let summaries the major viral diseases of Tomato in Tanzania and gives guidance on management options including IPM. It was produced as a training material for the East Africa Integrated Pest Management Innovation Lab (EAVC-IPM IL) project based at Mikocheni Agricultural Research Institute (MARI), Dar es salaam, Tanzania, with funding supp...
Article
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Cassava brown streak disease (CBSD) was first observed on cassava (Manihot esculenta Crantz) in Rwanda in 2009. In 2014 we surveyed eight major cassava-growing districts in the country to determine the distribution and variability of symptom phenotypes associated with CBSD, and the genetic diversity of Cassava brown streak viruses (CBSVs). Distribu...
Article
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Manihot carthaginensis subsp. glaziovii (Müll.Arg.) Allem., a wild relative of cassava, native to Brazil, is one of the popular agroforestry trees used for hedges and/or boundary plants surrounding homesteads and farms and also harbours cassava mosaic begomoviruses (CMBs) and cassava brown streak ipomoviruses. Sequences of the DNA-A component of Ea...
Article
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Illumina sequencing of RNA from a cassava cutting from northern Malawi produced a genome of Ugandan cassava brown streak virus (UCBSV-MW-NB7_2013). Sequence comparisons revealed stronger similarity to an isolate from nearby Tanzania (93.4% pairwise nucleotide identity) than to those previously reported from Malawi (86.9 to 87.0%).
Article
Full-text available
Background Cassava brown streak disease is emerging as the most important viral disease of cassava in Africa, and is consequently a threat to food security. Two distinct species of the genus Ipomovirus (family Potyviridae) cause the disease: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). To understand the evolutio...
Article
Full-text available
A novel bipartite legumovirus (genus Begomovirus, family Geminiviridae), that naturally infects the wild leguminous plant Desmodium sp. in Uganda, was molecularly characterized and named Desmodium mottle virus. The highest nucleotide identities for DNA-A, obtained from two field-collected samples, were 79.9% and 80.1% with the legumovirus, soybean...
Article
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Four isolates of a bipartite begomovirus from naturally infected Deinbollia borbonica plants exhibiting yellow mosaic symptoms in Kenya and Tanzania were molecularly characterised. The DNA-A was most closely related to that of tomato leaf curl Mayotte virus (AM701764; 82%), while the DNA-B shared the highest nucleotide sequence identity with that o...
Article
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The complete genomes of a monopartite begomovirus (genus Begomovirus, family Geminiviridae) and an associated betasatellite found infecting Vernonia amygdalina Delile (family Compositae) in Uganda were cloned and sequenced. Begomoviruses isolated from two samples showed the highest nucleotide sequence identity (73.1% and 73.2%) to an isolate of the...
Preprint
Full-text available
Background Bemisia tabaci species (whiteflies) are the world’s most devastating insect pests within crops in the tropics. They cause billions of dollars (US) of damage each year and are leaving farmers in the developing world food insecure. Understanding the genetic and transcriptomic composition of these insect pests, the viruses they transmit and...