Peter C K Cheung

Peter C K Cheung
The Chinese University of Hong Kong | CUHK · School of Life Sciences

Ph.D.

About

178
Publications
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Publications

Publications (178)
Article
β-d-glucans have the potential of serving as both macrophage-targeted carriers and immune stimulators via inducing trained immunity in macrophages. In this study, a carboxymethylated β-glucan from mushroom sclerotium of Pleurotus tuber-regium (CMPTR) was combined with iron oxide nanoparticles (IONPs) to form nanocomplexes (CMPTR/IONPs) with particl...
Article
Hyperbranched polysaccharide from Pleurotus tuber-regium (PTR-HBPS) is a β-glucan with high degree of branching (DB, 0.69) and a molecular weight (Mw) of 31.2 × 10⁵ g/mol with mixed β-(1, 4)-/β-(1, 4, 6)-/β-(1, 6)-glucosidic linkages. PTR-HBPS was depolymerized by cellulase and β-glucosidase under optimized conditions to form PC (PTR-HBPS depolymer...
Article
Hyperbranched polysaccharides (HBPSs) are the main components in cell wall and exopolysaccharide (EPS) of Pleurotus tuber-regium. To enhance the yield of these macromolecules, corn oil at 4% addition exhibited the best effect for production of mycelial biomass at 20.49 g/L and EPS at 0.59 g/L, which was 2.56 folds and 1.90 folds of the control, res...
Article
Use of polysaccharides as carriers in drug delivery system is a hot topic, especially those with specific recognition of immune cells, enabling them to be applied in targeting delivery system. β-d-glucans are naturally occurring non-digestible polysaccharides with immunomodulatory activities that have attracted increasing attention to serve as ther...
Article
Coprinopsis cinerea is a model mushroom-forming basidiomycete which produces basidiospores during sexual reproduction. This fungus is widely used to study fruiting body formation and development. Molecular mechanisms controlling its growth from vegetative mycelium to multicellular mature fruiting body have been studied extensively. However, little...
Article
Circadian rhythm governs multiple behavioural and physiological processes and its disruption is closely associated with various pathological conditions. In this study, the effects of dietary intervention by prebiotics including beta-glucan and inulin on attenuating circadian desynchrony in C57BL/6J mice subjected to weekly shifted light-dark cycle...
Article
β-d-glucan is a natural non-digestible polysaccharide that can be selectively recognized by recognition receptors such as Dectin-1 receptors, resulting in an emerging interest on exploring its capacity for carrying biological information to desired organs or cells. CpG oligodeoxynucleotide (ODN) has the potentiality to initiate an immune-stimulator...
Article
Beta-glucans as food hydrocolloids have demonstrated prebiotic potential recently. Novel hydrocolloid-based prebiotics could be developed by structural manipulation of existing carbohydrate candidates using different physical, chemical and/or biological approaches. However, there are no guidelines on choosing the most promising structural feature a...
Article
Full-text available
We proposed a framework with parametric modeling to obtain biological relevant parameters from the total probiotic growth pattern and metabolite production curves. The lag phase, maximum increase rate, and maximum capacity were obtained via a 205-h exploratory in vitro fermentation of a library of 13 structural-characterized prebiotic candidates ag...
Article
Full-text available
Naturally-occurring β-glucans are mostly investigated for their antitumor activity and immunomodulatory property. They have been widely regarded as a natural source for functional foods and pharmaceuticals. However, the physico-chemically stable and biocompatible properties of β-glucans are rarely explored as a coating material for nanomaterials to...
Article
The Human Microbiome Project has prompted unprecedented advancement to microbiome science. Personalized microbiome modulation with precision (PMMP) is one of the emerging yet challenging field in future microbiome research. Carbohydrate-based prebiotics (CBPs) have been shown to modulate gut microbiome in various extents by their different structur...
Article
This study evaluated the immunomodulatory effects of two high molecular weight and structurally different mushroom polysaccharides: an alkali-soluble one (mPRSon) and a water-soluble polysaccharide–protein complex (PRW) isolated previously from the sclerotia of Pleurotus rhinocerus on the maturation of murine bone-marrow-derived DCs (BMDCs). The ef...
Article
Full-text available
The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, Echinodontium tinctorium, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract...
Article
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Gallic acid (GA) is a natural anti-cancer compound that can be found in many food sources, including edible mushrooms, fruits, and vegetables. Studies generally attribute the anti-cancer activity of GA to the induction of apoptosis. Here, we reported that GA activated iron-dependent cell death mechanisms with apoptotic, ferroptotic, and necroptotic...
Article
In this study, an immunologically active novel microparticulate mushroom β-glucan (PRA-1p) was prepared using an alkali-soluble glucan PRA-1 by emulsification and cross-linking method. PRA-1 was a hyper-branched (1→3),(1→6)-β-D-glucan with a degree of branching of 0.89 isolated from the sclerotia of Polyporus rhinocerus. PRA-1 had a rod-like confor...
Article
In this study, a polysaccharide was extracted from Physalis pubescens L. (named PP). Its antihyperglycemic and antihyperlipidemic activities were evaluated in STZ-induced diabetic mice. Results showed that PP was determined to be composed of rhamnose (Rha), arabinose (Ara), fucose (Fuc), xylose (Xyl), mannose (Man), glucose (Glc) and galactose (Gal...
Article
In recent years, hyperbranched polymers, especially the natural hyperbranched polysaccharides (HBPSs), are receiving much attention due to their diverse biological activities and applications. With high degree of branching (DB), HBPSs mainly exist in the form of either a comb-brush shape, dendrimer-like particulate, or globular particle. HBPSs also...
Article
This study aims to evaluate the regulation of lipid metabolism and its mechanism of a sulfated polysaccharide from Gracilaria Lemaneiformis (GLP). The results showed that GLP could significantly decrease the levels of high-fat diet mice serum and hepatic total cholesterol (TC) and triglyceride (TG), prevent body weight gain and alleviate the accumu...
Article
Edible and medicinal mushrooms have usually been considered as a sustainable source of unique bioactive metabolites, which are valued as promising provisions for human health. Antrodia cinnamomea is a unique edible and medicinal fungus widespread in Taiwan, which has attracted much attention in recent years for its high value in both scientific res...
Article
Full-text available
Barley contains high level of β-1,3-1,4-glucans (BBGs) which can be fermented by microbes and are a potential prebiotic. In the present study, native BBG with low viscosity and a MW of 319 kDa was depolymerized by acid hydrolysis to produce a series of four structurally characterized fragments with MWs ranging from 6–104 kDa. In vitro fermentation...
Article
Full-text available
Objective The gut microbiota is an important influencing factor of metabolic health. Although dietary interventions with probiotics, prebiotics, and synbiotics can be effective means to regulate obesity and associated comorbidities, the underlying shifts in gut microbial communities, especially at the functional level, have not been characterized i...
Article
Commercial tools and instruments have been developed for screening study of microbial fermentation but they are expensive and mostly confined to aerobic fermentation only. There is little development on the generation of anaerobic condition directly on 96-well plate. This report proposed a simple and versatile microbial fermentation system known as...
Article
Two marine microalgae, Nitzschia closterium and Isochrysis zhangjiangensis, treated by a 9-day photosynthetically active radiation (PAR), were subjected to a 3-day ultraviolet light band A at 365 nm (UVA365) followed by a 3-day post-UVA PAR treatment. Compared to the control that only had PAR treatment, UVA365 treatment significantly (p<0.05) incre...
Article
Full-text available
This study was conducted to assess the potential application of ultrasonic treatment to enhance the tenderness of whelk (Buccinum undatum) meat. The optimum ultrasonic conditions for the maximum tenderization effect were determined using response surface methodology by a three‐level factorial Box–Behnken design for the optimization of three variabl...
Article
Pleurotus tuber-regium (Fr.) Singer, being a white-rot fungus, is widely used for food and medicine in the Asia-Pacific region. In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose...
Article
The anti-angiogenic activities of two structurally similar phenolics protocatechuic acid (PA) and syringic acid (SA) were investigated. In vitro study using HUVECs demonstrated that both PA and SA (at 25 μM) significantly (p < 0.05) inhibited VEGF-induced cell proliferation by 22.68 ± 5.6% and 21.93 ± 2.0%, respectively; cell migration by 50.04 ± 3...
Article
Full-text available
GPR43, a G-protein coupled receptor recognizing short-chain fatty acids, has been reported to participate in many biological functions of white adipocytes, such as adipogenesis and lipolysis. However, the functional role of GPR43 in brown adipocytes is still not clear. In this study, we investigated the effects of the PPAR γ agonist rosiglitazone o...
Data
Supplementary Figure 1: duration of XBP1 silencing after XBP1 siRNA transfection. IM-BAT cells were transfected with XBP1 siRNAs (Santa Cruz, no. sc38628) or control siRNA (sc-37007) at 200 nM. On the next day, cells were differentiated as indicated. Cells were lysed on day 0 through day 5 after differentiation and XBP1 transcription levels were me...
Article
Full-text available
Lignosus rhinocerotis has a long history of use by the indigenous community within East Asia to treat a range of health conditions including asthma and chronic cough. To date, there is limited scientific evidence to support its therapeutic effects in relieving these airways conditions. In this study, we examined the effects of the different molecul...
Article
The photothermal cancer therapeutic effect of the AuNR-Glu nanohybrids produced by coating the native gold nanorods (AuNRs) with a natural mushroom biopolymer from Pleurotus tuber-regium sclerotial β-glucan (Glu) were studied in the second near infrared window (NIR-II). AuNR-Glu showed low cytotoxicity and highly biocompatibility due to the surface...
Article
Two high molecular weight (MW larger than 788 kDa) mushroom polysaccharides extracted by hot water (PRW) and micronized sonication-assisted cold alkali (mPRSon), were isolated from the sclerotia of Polyporus rhinocerus. PRW was a polysaccharide-protein complex with a highly branched (degree of branching DB 0.60) β-D-mannoglucan while mPRSon was a h...
Article
Meat adulteration incidents have been reported frequently over the past few years, and the corresponding traceability issues attracted much more attention due to the customer's demands and administration's responsibility. Therefore, it is important to develop high-throughput and rapid detection methods to identify the specific sources from meat sam...
Article
A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229. kDa and is a heterog...
Article
Naturally-occurring β-glucans have been widely regarded as a natural source for functional foods and pharmaceuticals due to their immunomodulatory property and antitumor activity. However, physicochemically stable and the biocompatible β-glucans are rarely explored as a carrier for nanomaterials to overcome the problems of aggregation and nanotoxic...
Article
Prebiotics are being added to infant formula in order to mimic the effects of human milk oligosaccharides (HMOs) for the growth of probiotic bacteria especially bifidobacteria and lactobacilli in the infant gut. This preliminary study compares the in vitro fermentation of 13 different carbohydrates including monosaccharides, disaccharides, oligosac...
Article
Full-text available
Natural yellow pigments produced by submerged fermentation of Monascus purpureus have potential economic value and application in the food industry. In the present study, the relationships among fermentation conditions (in terms of pH and shaking/agitation speed), mycelial morphology and the production of Monascus yellow pigments were investigated...
Article
This study aimed at delineating the mechanism of the anti-angiogenic effect of chlorogenic acid. Chlorogenic acid demonstrated significant inhibitory effects on vascular endothelial growth factor (VEGF)-induced proliferation, migration, invasion and tube formation in human umbilical vein endothelial cells (HUVECs) while the quantitative endogenous...
Article
Naturally occurring carotenoids are important sources of antioxidants, anti-cancer compounds and anti-inflammatory agents and there is thus considerable market demand for their pharmaceutical applications. Carotenoids are widely distributed in marine and freshwater organisms including microalgae, phytoplankton, crustaceans and fish, as well as in t...
Article
Importance: Bifidobacterium strains are in general genetically intractable. Coupling classic forward genetics with next generation sequencing, we identified here an ABC transporter ATP-binding protein (MalK) responsible for the import of insoluble yeast glucan breakdown products by B. breve JCM1192. We demonstrated the pleiotropic effect of the AB...
Article
Full-text available
In this study, a large Clitocybe maxima mycelium biomass was obtained by submerged cultivation under optimal conditions. Three test samples from lyophilized mycelia, including hot water extract (CW) and elutes from solvents with different polarity (CA and CB), were combined and used to explore antioxidant and antihyperlipidemic activities in vitro...
Article
Marine microorganisms such as phytoplanktons are a rich resource of bioactive components with antioxidant and anti-proliferative activities that can act as novel functional food ingredients. In this study, the pigment profiles, total mycosporine-like amino acids (MAAs) and total phenolic contents (TPCs) in solvent extracts including 90% acetone and...
Article
Short-chain fatty acids (SCFAs), especially acetate, propionate and butyrate, are the end products from the intestinal microbial fermentation of dietary fibers and resistant starch. It has been well documented that plasma and colonic SCFAs are associated with metabolic syndromes. Recently, the involvement of SCFAs in energy homeostasis regulation h...
Article
Antroquinonol, an important bioactive metabolite from the edible mushroom Antrodia camphorata, was difficult to be synthesised by conventional submerged fermentation. The present study revealed the mechanisms related to the stimulatory effect of coenzyme Q0, camphorwood leach liquor and soybean oil on the biosynthesis of antroquinonol. Addition of...
Article
The water extract of the mushroom fruiting body of Agrocybe aegerita (AaE) had a total phenolic content of 13.67 ± 0.21 GAE (μM)/mg extract and 5 dominant phenolic acids including gallic acid, protocatechuic acid, chlorogenic acid, ferulic acid and sinapic acid were identified which accounted for 2.5% dry weight of the extract. AaE inhibited the va...
Article
A novel water-soluble polysaccharide-protein complex (PRW1) isolated from the sclerotia of an edible mushroom Polyporus rhinocerus which was purified by membrane ultrafiltration could significantly activate murine macrophages RAW264.7 in vitro. PRW1 had a molecular weight of less than 50 kDa and was found to be a highly branched heteropolysaccharid...
Article
An exopolysaccharide (PTR-EPS1) purified from the culture medium of the mycelial fermentation of Pleurotus tuber-regium had a weight-average molecular mass of 173.6 kDa and a radius of 55.6 nm. Methylation analysis and NMR results indicated that PTR-EPS1 had a 81% degree of branching at O-2 and consisted of a backbone of 1,6-linked α-d-Manp residue...
Article
The composition profile and the antioxidant properties of phenolics in water extracts obtained from the fresh fruiting bodies of 4 common cultivated Asian edible mushrooms—Agrocybe aegerita, Pleurotus ostreatus, P. eryngii, and Pholiota nameko were compared. The water extract from A. aegerita (AaE) had the highest total phenolic content (TPC) at 54...
Book
Full-text available
This handbook is intended to be a comprehensive reference for the various chemical aspects of foods and food products. Apart from the traditional knowledge, this book covers the most recent research and development of food chemistry in the areas of functional foods and nutraceuticals, organic and genetically modified foods, nonthermal food processi...
Article
An ethyl acetate fraction (EAF) from the mushroom sclerotium of Pleurotus tuber-regium (PTR) was rich in total phenolics (41.4 ± 0.67 mg/g extract) constituting 58% chlorogenic acid and 21% syringic acid. EAF had stronger antioxidant activity than other fractions in terms of 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzthiazoli...
Article
Water-soluble polysaccharides extracted from mushrooms have been found to have some physiological effects. In this study, exopolysaccharides (EPSs) were extracted by alcohol precipitation from cultivated broth of the mushroom Clitocybe maxima. EPSs with molecular weights of 104 and 105 Da were obtained by ultrafiltration; they are referred to as EP...
Chapter
Among the different branches of food science, food chemistry focuses on the changes in the composition and chemical, physical, and functional properties of foods and food products during their different processing stages and storage periods. Food chemistry has rapid development in the past few decades based on modern chemistry and biochemistry. Its...
Article
Pleurotus tuber-regium (Fries) Singer (PTR), both an edible and medicinal mushroom also known as tiger milk mushroom, has a growing popularity and economic importance due to its flavor, nutritive value and medicinal effects. In this study, the anti-oxidant and anti-angiogenic activities of a 60% ethanol extract (EE) obtained from the sclerotium of...
Article
Full-text available
Chemoprophylaxis and chemosensitization are promising strategies to combat human cancers. Natural antioxidant agents show great promise in cancer therapy, and the use of edible mushrooms against cancer is receiving more interest globally. In this study, the radical scavenging activities including diphenyl-1-picrylhydrazyl, superoxide anion radical,...
Article
The fine molecular structure of a novel alkali-soluble polysaccharide (SCA-I) from the mushroom sclerotial cell wall of Pleurotus tuber-regium was elucidated by 1-D and 2-D NMR spectroscopy as a hyper-branched glucan having a 64.9% degree of branching at O-6 and consisting a main chain with →1)-Glcp-(4→ linkages and short side chains having either...
Article
Cell wall of mushroom fruiting body is constituted of non-digestible macromolecules that are a rich source of dietary fiber with biological functions that are beneficial to human health. The cell wall components of an edible mushroom fruiting body from Pleurotus tuber regium (PTR) were fractionated and their chemical structures were investigated by...
Article
Full-text available
Dietary fiber and high-fiber food products have attracted great attention because of their significant health benefits to consumers. Mushrooms are valuable resources for food, medicine and nutraceuticals. Edible mushroom is considered as a novel source of dietary fiber. The dietary fiber content and composition in edible mushroom vary greatly with...
Article
Recent studies have demonstrated that β-glucans from different sources, which are considered as potential prebiotics, could enhance the growth of bifidobacteria. To elucidate the metabolic pathway of β-glucans in the widely used probiotic B. longumsubsp. infantis, a comparative proteomic analysis was carried out, along with two-dimensional differen...
Article
Two-dimensional gel electrophoresis identified 40 differentially expressed proteins which explained the mechanisms underlying the stimulatory effect of Tween 80 for exopolysaccharide production in the mycelium of an edible mushroom Pleurotus tuber-regium. The up-regulation of fatty acid synthase alpha subunit FasA might promote the synthesis of lon...
Article
Proteomic analysis was applied to investigate the mechanism of the stimulatory effect of Tween 80 on the mycelial growth and exopolysaccharide production by an edible mushroom Pleurotus tuber-regium. 32 differentially expressed proteins were identified by one-dimension gel electrophoresis. Combined with our previous findings, the up-regulation of h...
Article
Proteomic analysis was applied to investigate the mechanism of the stimulatory effect of Tween 80 on the mycelial growth and exopolysaccharide production by an edible mushroom Pleurotus tuber-regium. 32 differentially expressed proteins were identified by one-dimension gel electrophoresis. Combined with our previous findings, the up-regulation of h...
Article
Cell wall proteins (CWPs) play a vital role in the development of the different morphological stages including mycelium, fruiting body, and sclerotium in mushrooms which are important human food sources. Using fractionation by detergents and reducing agents, mushroom cell wall fractions from the different developmental stages of Pleurotus tuber-reg...
Article
Cell wall proteins (CWPs) play a vital role in the development of the different morphological stages including mycelium, fruiting body, and sclerotium in mushrooms which are important human food sources. Using fractionation by detergents and reducing agents, mushroom cell wall fractions from the different developmental stages of Pleurotus tuber-reg...
Article
Full-text available
Liver cancer or hepatocellular carcinoma is one of the leading causes of cancer-related deaths. Conventional chemotherapies are limited by the development of drug resistance and various side effects. Because of its non-toxicity and potent biopharmacological activity, metabolites derived from mushrooms have received more attention in cancer therapy....
Data
Potential cytotoxic activity of PP in normal cells. A. MTT assay was applied to determine the potential cytotoxic effect of PP against normal liver cell line WRL-68. B. Colony-formation assay demonstrated that WRL-68 cells are more resistant to PP than liver cancer cells. (PDF)
Data
No obvious inhibitory effect in liver cancer cell proliferation upon treatment with extract of another Pleurotus mushroom, Pleurotus tuber-regium. MTT assay was applied with polysaccharide and protein complex from another mushroom Pleurotus tuber-regium (PTR) as control. (PDF)