Peter A. Behnisch

Peter A. Behnisch
BioDetection Systems | BIODETECTIONSYSTEMS · International Research

Dr. rer. nat.
In vitro toxicity profiling of emerging pollutants (PFAS, EDC, POPs) in water, food, plastic, wildlife & human health.

About

130
Publications
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Introduction
My research is driven by a fascination for non-animal-, effect-, group- and human-cell based bioanalysis for environmental, food, plastic, chemicals, wildlife, public health & safety. More than 100 international publications & cases about pollutants (dioxins, PCBs, POPs, EDCs, PFAS) & their impact on environment, wildlife & humans. Director at BioDetection Systems in Amsterdam. Working experiences in Germany (SGS, Eurofins, Uni Tuebingen), Japan (Kaneka, Kyoto Uni ) and England (Lancaster Uni).
Additional affiliations
January 2006 - March 2021
BioDetection Systems
BioDetection Systems
Position
  • Managing Director
January 2006 - April 2021
BioDetection Systems
BioDetection Systems
Position
  • Managing Director

Publications

Publications (130)
Article
Full-text available
This review compiles information on PCDD/F- and PCB-contaminated eggs from 20 years of global egg monitoring around emission sources conducted by the International Pollutants Elimination Network (IPEN) and Arnika as well as a compilation of data from scientific literature. IPEN monitored 127 pooled egg samples including samples from 113 chicken flo...
Article
Full-text available
rationale of the study Three steroidal estrogens, 17α-ethinylestradiol (EE2), 17β-estradiol (E2), estrone (E1), and the non-steroidal anti-inflammatory drug (NSAID), diclofenac have been included in the first Watch List of the Water Framework Directive (WFD, EU Directive 2000/60/EC, EU Implementing Decision 2015/495). This triggered the need for mo...
Article
The suitability of the AhR reporter gene bioassays to screen the presence of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dl-PCBs) in sewage sludge (SL) and related hydrochar (HC) was here investigated. Samples of SL obtained from six WWTPs were processed by hydrothermal carbonization to obtain the r...
Article
Full-text available
A topsoil sample obtained from a highly industrialized area (Taranto, Italy) was tested on the DR-CALUX® cell line and the exposed cells processed with proteomic and bioinformatics analyses. The presence of polyhalogenated compounds in the topsoil extracts was confirmed by GC-MS/MS analysis. Proteomic analysis of the cells exposed to the topsoil ex...
Article
Full-text available
A set of CALUX in vitro bioassays was applied for long-term toxicity monitoring at an advanced wastewater treatment plant comprising ozonation and granular activated carbon filtration for the abatement of contaminants of emerging concern (CEC). During the 13-month monitoring, eight reporter gene assays targeting different modes of action along the...
Conference Paper
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PFAS in soil samples from Austria measured by TTR TR CALUX for their thyroid hormone disrupting properties. PFOA equivalents are similar between measured results by TTR TR CALUX and calculated by chemical analysis.
Article
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Persistent Organic Pollutants (POPs) contamination in developing countries can include domestic and foreign pollution sources. The study focused on sites potentially polluted by such sources on Java, Indonesia, in particular, areas affected by plastic and paper waste imports, secondary aluminium production, and waste incineration in five locations...
Article
Wastewater treatment plants (WWTPs) remain an important primary source of emission for endocrine-disrupting compounds in the environment. As an advanced wastewater treatment process, ozonation is known to reduce endocrine-disrupting activity. However, it remains unclear to which extend improved wastewater treatment may reduce the endocrine-disrupti...
Article
Full-text available
Background Over the last decade, per- and polyfluoroalkyl substances (PFASs) have become one of the most heavily investigated persistent organohalogen compound class of environmental concern. However, knowledge about their toxicology is still scarce, although PFASs as individual compounds and their industrial mixtures were shown to exert effects on...
Book
Full-text available
PFAS in the various environment samples in Vorarlberg, Austria measured by chemical (IOF, TOP assay and LCMS) and biological in vitro toxicity testing (TTR TR CALUX).
Article
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Durch natürliche und industriell hergestellte Lebensmittel sind wir einer komplexen Mischung aus bekannten und (noch) unbekannten Schad- und Wirkstoffen ausgesetzt. Auch Lebensmitteladditive haben unsere Lebensmittelqualität verändert und die daraus resultierenden biologischen Wirkungen auf Mensch und Tier sind oft schwer einzuschätzen. Menschen un...
Article
Full-text available
Background: Per- and polyfluoroalkyl substances (PFAS), organophosphate esters (OPEs), and polybrominated diphenyl ethers (PBDEs) are hormone-disrupting chemicals that migrate from building materials into air and dust. Objectives: We aimed to quantify the hormonal activities of 46 dust samples and identify chemicals driving the observed activities....
Article
Full-text available
Over the last decades, short-chain chlorinated paraffins (SCCPs), medium-chain chlorinated paraffins (MCCPs), and long-chain chlorinated paraffins (LCCPs) have become the most heavily produced monomeric organohalogen compound class of environmental concern. However, knowledge about their toxicology is still scarce, although SCCPs were shown to have...
Article
The aim of the present study was to assess the occurrence and spatial distribution of PCDD/Fs and dioxin-like compounds in topsoils of Taranto (Apulia Region), one of the most heavily industrialized and contaminated area of Southern Italy. A combined approach of chemical analysis by GC-MS/MS and AhR reporter gene bioassay was applied in a subset of...
Article
Full-text available
The anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not p...
Article
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Effect biomarkers can be used to elucidate relationships between exposure to environmental chemicals and their mixtures with associated health outcomes, but they are often underused, as underlying biological mechanisms are not understood. We aim to provide an overview of available effect biomarkers for monitoring chemical exposures in the general a...
Article
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Genotoxicity assessment is of high relevance for crude and refined petroleum products, since oil compounds are known to cause DNA damage with severe consequences for aquatic biota as demonstrated in long-term monitoring studies. This study aimed at the optimization and evaluation of small-scale higher-throughput assays (Ames fluctuation, micronucle...
Article
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Brominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are increasingly reported at significant levels in various matrices, including consumer goods that are manufactured from plastics containing certain brominated flame retardants. PBDD/Fs are known ligands for the aryl hydrocarbon receptor (AhR) but are not yet considered in the hazard assessme...
Article
Full-text available
At the beginning of the first dioxin crises at the end of the last century, we did not anticipate that they would continue to pop up as a problem in the world for so long and that so many unexpected dioxin sources existed in the feed and food sector (see the figure on page 46). Over time, we have learned many lessons about how dioxins can enter the...
Poster
Full-text available
Brominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are increasingly reported at significant levels in various matrices, including consumer goods that are manufactured from black plastics. PBDD/Fs are known ligands for the dioxin receptor (AhR) but are not yet considered in the hazard assessment of dioxin mixtures. The aim of the present study...
Conference Paper
Full-text available
Brominated dioxins (PBDD/Fs) have been found to exhibit similar toxicity and health effects as their chlorinated analogues (PCDD/Fs). They can for example affect brain development, damage the immune system and fetus or induce carcinogenesis. In general, brominated dioxins are subject to significantly less regulation than chlorinated dioxins. For ex...
Conference Paper
Full-text available
The urinary excretion of the AAS metabolites required the combination with glucuronic acid, that phase II metabolic modification is controlled by an enzyme that can by affected in its performance by a deletion of the UGT2B17 gen. According to published data the prevalence of this deletion among Asiatic is 70% and Caucasians 7%. The potential of BDS...
Conference Paper
Full-text available
There is a range of studies on PCDD/Fs and PCBs in eggs. Eggs have been found to be sensitive indicators of POPs contamination in soils and are an important exposure pathway from soil pollution to humans. In this study, we sought to broaden the available data and eggs were sampled at sites suspected of being impacted by POPs in countries of Centra...
Article
Full-text available
Incineration of medical waste and open burning of waste including electronic waste are potentially large sources of toxic chemicals known as persistent organic pollutants (POPs). These substances are slated for global reduction and elimination under the Stockholm Convention. Medical waste incineration and open burning are common in developing count...
Article
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Decabromodiphenyl ether (DecaBDE) is the last of the polybrominated diphenyl ethers (PBDEs) added to the list of brominated flame retardants (BFRs) that are banned and/or regulated by the Stockholm Convention on Persistent Organic Pollutants (POPs). As such it should also have an international limit for its content in wastes. That limit is the Low...
Conference Paper
Full-text available
Food Contact Materials FCM) are essential in the food packaging. According to the European Food Safety Agency, some of the chemicals or particles that can migrate from Food Contact Materials are suspected to have Endocrine Disrupting properties. Overall migration of the finished packaging could be evaluated for biological effects using a wide rang...
Technical Report
Full-text available
This study investigated POPs contamination at a total of six sites: the world’s largest e-waste scrap yard in Agbogbloshie (Ghana); medical waste incinerators in Accra (Ghana), Kumasi (Ghana) and Yaoundé (Cameroon); and two open-burning waste dump sites in Yaoundé (Cameroon). The study measured POPs in eggs because free-range chickens are “active s...
Article
Averaged 7-day composite effluent wastewater samples from twelve wastewater treatment plants (WWTPs) in nine countries () in the Danube River Basin were collected. WWTPs' selection was based on countries' dominant technology and a number of served population with the aim to get a representative holistic view of the pollution status. Samples were an...
Conference Paper
Full-text available
Chemical and bioanalytical analyis of thyroid hormone disrupting properties of PFAS in food samples from an international interlaboratory study. PFOA in vitro toxic equivalents measured by PFAS CALUX and calculated from chemical analysis of a small number of standard PFAS congeners. Highly PFAS polluted fish shows a 300-times higher PFOA equivalent...
Article
Full-text available
Member States of the EU are required to monitor the use of pharmacologically active substances in food-producing animals. There is evidence, however, that the target-based approach currently applied in official monitoring plans might under-estimate the real incidence of growth promoter abuse in livestock. As demonstrated for sex hormones, the assoc...
Book
Full-text available
Thailand has undergone major industrial and social transformation amid rapid economic growth and development for over half a century. It has successfully shifted its economy from agriculture to export-oriented manufacturing, while integrating key production, particularly automobiles and electronics, into regional value chains. Toxic pollution, as t...
Book
Full-text available
This study found significant levels of toxic brominated dioxins (PBDD/Fs) accompanying brominated flame retardants in nine samples of consumer products made from recycled plastics. PBDD/Fs are as toxic as the better-known chlorinated dioxins and furans (PCDD/Fs). In addition, PBDEs and PBDD/Fs are endocrine disrupting chemicals, which may impact th...
Article
Equine Metabolic Syndrome (EMS) is characterized by abnormalities in insulin regulation, increased adiposity and laminitis, and has several similarities to human metabolic syndrome. A large amount of environmental variability in the EMS phenotype is not explained by commonly measured factors (diet, exercise, and season), suggesting that other envir...
Conference Paper
Full-text available
There are a range of studies on PCDD/Fs and PCBs in eggs. Eggs have been found to be sensitive indicators of PCDD/F and PCB contamination in soils and are an important exposure pathway from soil pollution to humans. Eggs from contaminated areas can readily lead to exposures which exceed thresholds for the protection of human health. IPEN, an intern...
Conference Paper
Full-text available
Introduction : Les cas d'utilisation des mélanges des cendres volantes et des cendres résiduelles provenant de l'incinérateur de déchets Byker pour le pavage des trottoirs entre les lotissements à Newcastle aux Royaumes unis entre 1994 et 1999 ont provoqué une contamination grave de la volaille élevée en liberté par les PCDD/PCDF 1,2. Ils ont aussi...
Conference Paper
Full-text available
Introducción: El caso del uso de cenizas volantes mixtas y cenizas de fondo del incinerador de residuos Byker (WI) para pavimentar senderos entre parcelas en Newcastle, Reino Unido, entre 1994 y 1999 condujo a una grave contaminación de aves de corral criadas en libertad por PCDD/Fs 1,2. También generó mayor concientización sobre el uso de residuos...
Article
Full-text available
There are a range of studies on PCDD/Fs and PCBs in eggs1-7. Eggs have been found to be sensitive indicators of PCDD/F and PCB contamination in soils and are an important exposure pathway from soil pollution to humans. Eggs from contaminated areas can readily lead to exposures which exceed thresholds for the protection of human health. Chickens and...
Article
Full-text available
The case of use of mixed fly ash and bottom ash from the Byker waste incinerator (WI) for paving footpaths between allotments in Newcastle, UK between 1994 and 1999 led to serious contamination of free range poultry by PCDD/Fs1,2. It also raised awareness about use of WI residues contaminated by PCDD/Fs. Similar cases of “Kieselrot” in Germany led...
Article
The European Decision EU 2015/495 included three steroidal estrogens, estrone, 17β-estradiol and 17α-48 ethinyl estradiol, in the “watch-list” of the Water Framework Directive (WFD). As consequence, these substances have to be chemically monitored at the level of their environmental quality standards, which can be challenging. This project aimed to...
Article
Full-text available
Background The European Commission Implementing Decision EU 2015/495 included three steroidal estrogens, namely 17α-ethinyl estradiol, 17β-estradiol, and estrone, in the so-called “watch list” of the EU Water Framework Directive (WFD). The monitoring of these compounds is difficult because the detection limits of the majority of the available anal...
Article
Effect-based methods including cell-based bioassays, reporter gene assays and whole-organism assays have been applied for decades in water quality monitoring and testing of enriched solid-phase extracts. There is no common EU-wide agreement on what level of bioassay response in water extracts is acceptable. At present, bioassay results are only ben...
Book
Full-text available
In this study, we present the results of the monitoring of the free-range chicken eggs from selected sites in Kazakhstan contamination by persistent organic pollutants (POPs). High levels of PCDD/Fs and DL PCBs were found in free range chicken egg samples. Four-fifths of the samples exceeded EU MAC for PCDD/Fs and DL PCBs in chicken eggs. More regu...
Conference Paper
Full-text available
Article
Wastewaters contain complex mixtures of chemicals, which can cause adverse toxic effects in the receiving environment. In the present study, the toxicity removal during wastewater treatment at seven municipal wastewater treatment plants (WWTPs) was investigated using an effect-based approach. A battery of eight bioassays was applied comprising of c...
Article
Full-text available
Heterocyclic aromatic amines (HCAs) are compounds formed when meat or fish are cooked at high temperatures for a long time or over an open fire. To determine which pathways of toxicity are activated by HCAs, nine out of the ten HCAs known to be carcinogenic in rodents (2-amino-9H-pyrido[2,3-b]indole (AαC), 2-aminodipyrido[1,2-a:3′,2-d]imidazole (Gl...
Book
Full-text available
In this study, we present the results of the monitoring of the free-range chicken eggs from selected sites in Kazakhstan contamination by persistent organic pollutants (POPs). High levels of PCDD/Fs and DL PCBs were found in free range chicken egg samples. Four-fifths of the samples exceeded EU MAC for PCDD/Fs and DL PCBs in chicken eggs. More reg...
Article
Endocrine Disruptors Chemicals (EDCs), are a wide and heterogeneous group of molecules, which includes persistent pollutants (dioxins), industrial chemicals (bisphenol-A and phthalates), natural substances (phytoestrogens) and pesticides, possibly linked to developmental and reproductive outcomes in children and adults. EDCs are hormonally active s...
Conference Paper
Full-text available
Tanzania experiences a rapid increase in industry, agriculture, as well asurbanization and population growth. The increasing use of chemicals and improper municipal waste treatment may result in discharge of potentially toxic chemicals, such as persistent organic pollutants (POPs). Human exposure to these chemicals are associated with adverse heal...
Conference Paper
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Article
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The role of the peregrine falcon (Falco peregrinus) as a bioindicator for the accumulation of organohalogen compounds and other persistent organic pollutants has been established in field studies. Biometric indices for damage caused by pollutants such as the shell thickness and the shell index were determined and the egg contents were analyzed for...
Book
Full-text available
POPs contamination in some free range chicken eggs from Bosnian and Serbian hot spots shows that there are significant sources of pollution by dioxins and dioxin like PCBs as well as significant levels of DDT in the environment at some places in Tuzla, Zenica, and the Obrenovac area in particular. High levels of PCDD/Fs and DL PCBs were found in fr...
Conference Paper
Full-text available
Contamination of eggs with polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/PCDF), polybrominated PBDD/PBDF or polychlorinated biphenyls (PCB) have been found around larger emission sources in different countries. The DRCALUX have been used as screening tool for monitoring dioxin-like toxicity in eggs. Contamination have been found around...
Article
Challenges to testing for the illicit use of anabolic substances in meat-producing animals stem from the production of new synthetic compounds and the administration of low-dose cocktails to circumvent detection by the surveillance schemes of European Union member states. This work evaluated for the first time GR-CALUX, a highly sensitive reporter...
Conference Paper
Full-text available
There have been an increasing number of reports on contamination of eggs with PCDD/Fs and particularly dl- PCBs in recent years3-7. Eggs have been found to be sensitive indicators of PCDD/F and PCB contamination in soils and are an important exposure pathway from soil pollution to humans and eggs from contaminated areas can readily lead to exposure...
Book
Full-text available
This series of studies is focused on the presentation and discussion of the data related to contamination of soils and sediments and pollution of free range chicken eggs, cow milk and fish by heavy metals and persistent organic pollutants. Environmental samples were obtained during two field visits conducted in Kazakhstan in July–August 2013 and Ju...
Article
Challenges to testing for the illicit use of anabolic substances in meat-producing animals stem from the production of new synthetic compounds and the administration of low-dose cocktails to circumvent detection by the surveillance schemes of European Union member states. This work evaluated for the first time GR-CALUX, a highly sensitive reporter...
Article
Full-text available
Fish is a major source for the intake of polybrominated diphenyl ethers (PBDEs). However, fish is scarcely consumed without being cooked, and previous studies showed that the heating of salmon fillet contaminated with BDE-209 for longer periods of time was accompanied with the partial transformation of this brominated flame retardant. In this study...
Article
A total of 318 local and imported meat, milk, eggs, fish, and animal feed samples collected in Kuwait were analyzed by cell-based reporter gene assay (Dioxin-Responsive Chemical Activated LUciferase gene eXpression DR-CALUX) for PCDD/Fs and dl-PCBs. The bioanalytical equivalents (BEQs) obtained by DR-CALUX bioassay were compared with the official m...
Article
Full-text available
Products of a thermal reaction of chlorpyrifos were analyzed by LC/MS and GC/MS. As a result, it was found that chlorpyrifos undergoes an elimination reaction of phosphate by a thermal reaction. Furthermore, it is indicated that the structural analogue of 2,3,7,8-TCDD arises by a thermal reaction of chlorpyrifos. In addition, the toxicity of the pr...