Pernilla Syrja

Pernilla Syrja
University of Helsinki | HY · Veterinary Pathology

DVM DECVP

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77
Publications
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Publications

Publications (77)
Article
Full-text available
Inherited neurodegenerative disorders are debilitating diseases that occur across different species. We have performed clinical, pathological and genetic studies to characterize a novel canine neurodegenerative disease present in the Lagotto Romagnolo dog breed. Affected dogs suffer from progressive cerebellar ataxia, sometimes accompanied by episo...
Article
Full-text available
Acute respiratory distress syndrome (ARDS) is the leading cause of death in critical care medicine. The syndrome is typified by an exaggerated inflammatory response within the lungs. ARDS has been reported in many species, including dogs. We have previously reported a fatal familial juvenile respiratory disease accompanied by occasional unilateral...
Article
Full-text available
Inherited ataxias are characterized by degeneration of the cerebellar structures, which results in progressive motor incoordination. Hereditary ataxias occur in many species, including humans and dogs. Several mutations have been found in humans, but the genetic background has remained elusive in dogs. The Finnish Hound suffers from an early-onset...
Article
Full-text available
A horse in Finland exhibited generalized granulomatous inflammation and severe proliferative dermatitis. After eu-thanization, we detected poxvirus DNA from a skin lesion sample. The virus sequence grouped with parapoxviruses, closely resembling a novel poxvirus detected in humans in the United States after horse contact. Our findings indicate hors...
Article
Idiopathic pulmonary fibrosis (IPF) in West Highland white terriers (WHWTs) is a breed-related, spontaneously arising disease that is a potential animal model for IPF in man. Histopathological similarity between IPF in WHWTs and usual interstitial pneumonia (UIP), the histopathological correlate for IPF in man, has not been confirmed and histologic...
Article
Full-text available
Background Conventional diagnostic methods have some limitations in diagnosing specific causes of canine hepatobiliary disorders. In the evaluation of the hepatobiliary system in dogs, ultrasonography (US) is the first imaging method of choice. Nonetheless, endoscopic retrograde cholangiopancreatography (ERCP) has also been proven to be a practicab...
Article
Full-text available
Background Gastric carcinoma (GC) is uncommon in dogs, except in predisposed breeds such as Belgian Shepherd dogs (BSD) of the Tervuren and Groenendael varieties. When GC is diagnosed in dogs it is often late in the disease, resulting in a poorer prognosis. The aim of this prospective clinical study was to investigate possible associations of gastr...
Article
Full-text available
We investigated the clinical, genetic, and pathological characteristics of a previously unknown severe juvenile brain disorder in several litters of Parson Russel Terriers. The disease started with epileptic seizures at 6–12 weeks of age and progressed rapidly to status epilepticus and death or euthanasia. Histopathological changes at autopsy were...
Article
Full-text available
Distinctive colour patterns in dogs are an integral component of canine diversity. Colour pattern differences are thought to have arisen from mutation and artificial selection during and after domestication from wolves but important gaps remain in understanding how these patterns evolved and are genetically controlled. In other mammals, variation a...
Preprint
Full-text available
We investigated the clinical, genetic, and pathological characteristics of a previously unknown severe juvenile brain disorder in several litters of Parson Russel Terriers. The disease started with epileptic seizures at 6 to 12 weeks of age and progressed rapidly to status epilepticus and death or euthanasia. Histopathological changes at autopsy we...
Preprint
Full-text available
Background: Conventional diagnostic methods have some limitations in diagnosing specific causes of canine hepatobiliary disorders. In the evaluation of the hepatobiliary system in dogs, ultrasonography (US) is the first imaging method of choice. Nonetheless, endoscopic retrograde cholangiopancreatography (ERCP) has also been proven to be a practica...
Article
The intestinal microbiota is believed to play a role in the pathogenesis of inflammatory bowel disease in humans and chronic inflammatory enteropathy (CIE) in dogs. While most previous studies have described the gut microbiota using sequencing methods, it is fundamental to assess the spatial distribution of the bacteria for a better understanding o...
Preprint
The intestinal microbiota is believed to play a role in the pathogenesis of inflammatory bowel disease in humans and chronic inflammatory enteropathy (CIE) in dogs. While most previous studies have described the gut microbiota using sequencing methods, it is fundamental to assess the spatial distribution of the bacteria for a better understanding o...
Conference Paper
Full-text available
Belgian Shepherd dogs (BSD) are considered at increased risk for gastric carcinoma (GC), mucous metaplasia and glandular dysplasia. In humans, gastric mucosal atrophy, metaplasia and dysplasia are all linked to GC. We performed a prospective clinical trial exploring early diagnosis of such conditions. Gastroscopy with histology of mucosal biopsies...
Article
Objective: To quantify the peripheral selectivity of vatinoxan (L-659,066, MK-467) in dogs by comparing the concentrations of vatinoxan, dexmedetomidine and levomedetomidine in plasma and central nervous system (CNS) tissue after intravenous (IV) coadministration of vatinoxan and medetomidine. Study design: Experimental, observational study. An...
Article
Full-text available
Inherited skeletal disorders affect both humans and animals. In the current study, we have performed series of clinical, pathological and genetic examinations to characterize a previously unreported skeletal disease in the Karelian Bear Dog (KBD) breed. The disease was recognized in seven KBD puppies with a variable presentation of skeletal hypomin...
Article
Full-text available
Background: Matrix metalloproteinases (MMPs) 2 and 9 are zinc- and calcium-dependent endopeptidases involved in the breakdown and reconstitution of extracellular matrix under both physiological and pathological conditions. Mucosal MMP-2 and -9 activities have been reported to be upregulated in the intestine of humans with inflammatory bowel diseas...
Article
Full-text available
Background: Gastric carcinoma (GC) is a rather rare pathological finding in dogs, with the exception of some breeds which seem predisposed. The etiopathogenesis is largely unknown in dogs, whereas in humans GC often develops from gastric mucosal metaplasia and dysplasia. This study investigates whether dogs of certain breeds are more often subject...
Article
Full-text available
Background: Intestinal absorption of bile acids is mediated by the apical sodium-dependent bile acid transporter (ASBT). Fecal bile acid dysmetabolism has been reported in dogs with chronic inflammatory enteropathy (CIE). Objective: Characterization of ASBT distribution along the intestinal tract of control dogs and comparison to dogs with CIE....
Article
Full-text available
Background: Intestinal mucosal S100A12 and myeloperoxidase (MPO) are inflammatory biomarkers in humans with inflammatory bowel disease (IBD). However, these biomarkers have not been studied in the intestinal mucosa of dogs with chronic enteropathies (CE), even though dogs with CE have increased S100A12 concentrations in feces and serum. This study...
Article
Mycobacteriosis caused by non-tuberculous mycobacteria (NTM) is a rising concern in human medicine both in immunocompromised and immunocompetent patients. In cats, mycobacteriosis caused by NTM is considered mostly to be a focal or dermal infection, with disseminated disease mostly caused by Mycobacterium avium. We describe three cases of dissemina...
Article
Full-text available
Objective To investigate the clinical, cytological, and histopathological adverse effects of intra-articularly injected botulinum toxin A in dogs and to study whether the toxin spreads from the joint after the injection. Methods A longitudinal, placebo-controlled, randomized clinical trial was conducted with six healthy laboratory Beagle dogs. Sti...
Data
Painless range of motion of stifle joints of healthy Beagle dogs after intra-articular botulinum toxin A or placebo. (DOCX)
Data
Dynamic weight-bearing of hind limbs in healthy Beagle dogs after intra-articular botulinum toxin A and placebo. (DOCX)
Data
Static weight-bearing of hind limbs of healthy Beagle dogs after intra-articular botulinum toxin A or placebo. (DOCX)
Data
Supporting Information Table S1 Correlation between ASBT expression, clinical, laboratory, and histopathological findings in dogs with CIE.
Conference Paper
Full-text available
S100A12 and myeloperoxidase (MPO) are considered inflammatory markers of chronic enteropathy (CE) and mainly originate from granulocytes. Increased concentrations of canine S100A12 (cA12) in feces and serum were found in dogs with CE. Intestinal mucosal MPO activities have been reported to be increased in humans with inflammatory bowel disease (IBD...
Article
Full-text available
A missense variant in the autophagy-related ATG4D-gene has been associated with a progressive degenerative neurological disease in Lagotto Romagnolo (LR) dogs. In addition to neural lesions, affected dogs show an extraneural histopathological phenotype characterized by severe cytoplasmic vacuolization, a finding not previously linked with disturbed...
Article
Neutrophilic and lymphocytic cholangitis are common forms of liver diseases in cats. Diagnosing these diseases can be difficult du to varying clinical picture. BIochemical analysis of serum may show changes typical to liver diseases. Diagnostic imaging is of little benefit. A prcise diagnosis is necessary so that these two specific diseases can be...
Article
Within a ten year period, cystoscopies were performed for 47 dogs at the Small Animal Veterinary Teaching Hospital. The dogs in the study represented 33 different breeds. Most of the procedures were performed for female dogs (91%). The majority of the patients presented multiple clinical signs (62%). The most common were pollakiuria (49%), strangur...
Article
Full-text available
A horse in Finland exhibited generalized granulomatous inflammation and severe proliferative dermatitis. After euthanization, we detected poxvirus DNA from a skin lesion sample. The virus sequence grouped with parapoxviruses, closely resembling a novel poxvirus detected in humans in the United States after horse contact. Our findings indicate horse...
Data
Technical Appendix. Summary of PCR results on a sample from a horse infected with possible novel parapoxvirus in Finland, 2013, and image of the horse.
Article
Full-text available
Background: Relatively few laboratory markers have been evaluated for the detection or monitoring of intestinal inflammation in canine chronic enteropathies, including inflammatory bowel disease (IBD). Previous research found that the intestinal mucosal levels of S100A12 and myeloperoxidase (MPO), as biomarkers of gut inflammation, were elevated i...
Article
Activins, cytokines belonging to the transforming growth factor-β superfamily, have an important role in inflammation and fibrosis. Activin A has been suggested to participate in the pathophysiology of human idiopathic pulmonary fibrosis (IPF), but studies on the role of activin B are sparse. Canine IPF (CIPF) is an incurable interstitial lung dise...
Conference Paper
Matrix metalloproteinases (MMPs) 2 and 9 are zinc-dependent endopeptidases that contribute to the control of breakdown and reconstitution of extracellular matrix under both normal and pathological conditions. Intestinal mucosal levels of MMP-2 and -9 have been shown to be increased in animal models and human IBD. To our knowledge, the presense of M...
Article
Full-text available
Matrix metalloproteinases (MMPs) 2 and 9 are zinc-dependent endopeptidases that contribute to the control of breakdown and reconstitution of extracellular matrix under both normal and pathological conditions. The main objective of this study was to identify the presence of MMP-2 and -9 in the mucosa of the small and large intestines of clinically h...
Article
Background: Idiopathic pulmonary fibrosis (IPF) is an incurable interstitial lung disease occurring mainly in West Highland White Terriers (WHWTs). The effects of IPF on survival and on exercise tolerance in WHWTs are unknown. Objectives: To evaluate survival, prognostic factors, and exercise tolerance in WHWTs with IPF. Animals: Privately own...
Article
Congenital hypothyroidism and dysmaturity syndrome (CHD) in neonatal foals was first described in western Canada in 1981 and remains an important problem for the western Canadian horse industry. This syndrome is characterised by hyperplasia of the thyroid gland, increased gestational length and multiple congenital musculoskeletal abnormalities. Thi...
Article
Activation of transforming growth factor (TGF)-β is a key event in the progression offibrosis in human lung tissue. Idiopathic pulmonary fibrosis (IPF) in West Highland white terriers (WHWTs) shares histopathological features of human usual interstitial pneumonia (UIP), the histopathological counterpart of IPF, and non-specific interstitial pneumon...
Data
Microsatellite marker primers. (XLSX)
Data
Gait. Video shows the normal gait of an unaffected three months old Finnish Hound puppy and the altered gait of two affected similarly aged puppies. Note the difficulty of the affected puppies to control limb movements (ataxia), particularly in the hind limbs. Affected puppies present with swaying of the whole body (truncal ataxia) and abnormal pos...
Data
Intention tremor. Video shows four affected puppies that suffer from intention tremor, which is a type of tremor never seen in healthy puppies. The tremor is predominantly seen when movement is initiated (first puppy) or when there is an intention to eat or smell (other three puppies). The amplitude of the intention tremor of the head varies from l...
Data
Full-text available
Variants identified in candidate gene sequencing. (PDF)
Data
Candidate gene sequencing primers. (XLSX)
Data
Full-text available
Ataxia candidate genes. (PDF)
Data
Control dogs from other breeds. (PDF)
Data
Primers for quantitative PCR. (XLSX)
Data
Postural reactions. Video shows postural reactions in an unaffected and affected puppy. Postural reactions were examined by testing the puppies' proprioception (overknuckling), hopping reaction and postural thrust reaction, and by performing the wheelbarrowing test (tests appear in this order in the video). Proprioceptive positioning is normal in b...
Article
Full-text available
A 3-year-old Cairn Terrier dog that had been in contact with sea water containing cyanobacteria (blue-green algae) was euthanized because of acute hepatic failure and anuria after a 5-day illness. Histologic findings included lytic and hemorrhagic centrilobular hepatocellular necrosis and renal tubular necrosis. The cyanotoxin nodularin was detecte...
Article
Full-text available
The macrolid antibiotic tylosin has been widely used to treat canine chronic diarrhea, although its efficacy is based on anecdotal reports and experimental studies in dogs and not on strong scientific evidence. The term tylosin-responsive diarrhea (TRD) refers to diarrheal disorders responding to tylosin therapy within a few days. In TRD, the stool...
Article
We use endoscopy for VIS-NIR spectral analysis of the porcine gastrointestinal wall. A flexible endoscope was implemented with a fiber optic probe for measuring spectra. The spectral data were measured by a spectrophotometer (Ocean Optics HR4000). The presented measurements are the spectra from three locations of the gastrointestinal wall of a heal...
Article
We use endoscopy for VIS-NIR spectral analysis of the porcine gastrointestinal wall. A flexible endoscope was implemented with a fiber optic probe for measuring spectra. The spectral data were measured by a spectrophotometer (Ocean Optics HR4000). The presented measurements are the spectra from three locations of the gastrointestinal wall of a heal...
Article
The histopathological changes in the lungs of 12 related Dalmatians with idiopathic acute respiratory distress syndrome (ARDS) are described. Affected dogs had multiple foci of marked atypical hyperplasia and squamous metaplasia of the bronchiolar epithelium, patchy ongoing fibrosis with myofibroblastic metaplasia, smooth muscle hyperplasia and occ...
Article
A neurological disorder characterised by pelvic limb metatarsophalangeal joint extensor paresis has been observed in numerous horses in Scandinavia for the last decade. Very little has been formally reported and there have been no detailed assessments of the neurological signs or neuropathological lesions. To describe the epidemiological and pathol...
Article
This case report documents two pathological variations of potentially inherited, cerebellar cortical abiotrophy in two unrelated Lagotto Romagnolo breed dogs. The first dog had an atypical lesion in the cerebellar cortex with depletion of cerebellar granular cell layer and sparing of the Purkinje cell layer. The second case had degenerative changes...