Periandavan Kalaiselvi

Periandavan Kalaiselvi
University of Madras | UMadras · Department of Medical Biochemistry

M.Sc., Ph.D.

About

82
Publications
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1,399
Citations
Additional affiliations
September 1999 - present
University of Madras
Position
  • Professor (Assistant)

Publications

Publications (82)
Article
Cardiac function demands a combination of iron availability for its high energy requirement and tight regulation of redox signaling. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a master transcriptional regulator of antioxidant/cytoprotective genes and recent reports has emphasized its role in regulating iron metabolism and ferroptosis in...
Article
Type 1 diabetes is a chronic immune‐mediated disease caused by pancreatic β‐cell dysfunction with consequent severe insulin deficiency. Exacerbated blood glucose levels can cause oxidative stress in the pancreatic β‐cells, which leads to inflammation, and apoptosis resulting in islet dysfunction. Although massive studies have been carried out to el...
Article
Background: Endogenous pancreatic β-cell regeneration is a promising therapeutic approach for enhancing β-cell function and neogenesis in diabetes. Various findings have reported that regeneration might occur via stimulating β-cell proliferation, neogenesis, or conversion from other pancreatic cells to β-like cells. Although the current scenario i...
Article
Iron is an essential trace element required for several vital physiological and developmental processes, including erythropoiesis, bone, and neuronal development. Iron metabolism and oxygen homeostasis are interlinked to perform a vital role in the functionality of the heart. The metabolic machinery of the heart utilizes almost 90 % of oxygen throu...
Article
Diabetes Mellitus is a metabolic disorder characterized by elevated blood glucose level caused due to deficiency of insulin secretion or insulin action. The management of the blood glucose level is an important approach in the control of diabetes mellitus complications. There are many divergent therapeutic strategies in the management of Type II di...
Article
Full-text available
Cardiac hypertrophy is the underlying cause of heart failure and is characterized by excessive oxidative stress leading to collagen deposition. Therefore, understanding the signalling mechanisms involved in excessive extracellular matrix deposition is necessary to prevent cardiac remodelling and heart failure. In this study, we hypothesized that he...
Article
Type 2 diabetes is delineated by impaired metabolic flexibility, and intramyocellular lipid accumulation, causing insulin resistance, particularly in skeletal muscle by reducing insulin-stimulated glucose uptake. High-fat diet and high fructose (HFD and HF) administration in rodents bestows a model for hyperlipidemia, insulin resistance, and Type 2...
Article
The aim is to test the hypothesis whether the cholesterol loaded lysosomes are capable of mediating lysosomal membrane permeabilization (LMP) during aging and to study the efficacy of epigallocatechin‐3‐gallate (EGCG) in preserving the lysosomal membrane stability. Aged rats were fed with high cholesterol diet (HCD) and treated with EGCG orally. Se...
Article
Background: Hyperoxaluria and calcium oxalate crystal deposition in the kidneys lead to overproduction of reactive oxygen species, resulting in the development of oxidative stress and renal injury. At the cellular levels, mitochondria and NADPH oxidase involved in reactive oxygen species production play a crucial role in the pathogenesis of hyperox...
Article
Full-text available
Background: Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is the major receptor for oxidized low-density lipoprotein (Ox-LDL) in the aorta of aged rats. Ox-LDL initiates LOX-1 activation in the endothelium of lipid-accumulating sites of both animal and human subjects of hypercholesterolemia. Targeting LOX-1 may provide a novel di...
Article
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Given the role of oxidative stress in PD pathogenesis and off-target side effects of currently available drugs, several natural phytochemicals seem to be promising in the management of PD. Here, we tested the hypothesis that scopoletin, an active principle obtained from Morinda citrifolia (MC), efficiently quenches oxidative stress through DJ-1/Nrf...
Preprint
Full-text available
Objective To arrive at apposite risk prediction strategies in identifying markers that could delineate patients susceptible for atherosclerotic progression from other diabetic patient population thus circumventing undesirable drug exposure or conversely inadequate treatment. Research Design and Methods The study was designed to assess and compare...
Article
Background Cardiac hypertrophy characterized by excessive oxidative stress leading to collagen deposition, predisposes the heart to failure (HF) if left unresolved. Thus, understanding the signaling mechanisms involved in excessive extracellular matrix deposition is necessary to prevent cardiac remodeling and HF. Here, we tested a hypothesis that h...
Preprint
Given the role of oxidative stress in PD pathogenesis and off-target side effects of currently available drugs, several natural phytochemicals seems to be promising in the management of PD. Here, we tested the hypothesis that scopoletin, an active principle obtained from Morinda citrifolia (MC), efficiently quenches oxidative stress through DJ-1/Nr...
Chapter
Phytochemicals are diverse secondary metabolites derived from plants, and it has been proven that phytochemicals can extend longevity by evolutionarily conserved mechanisms. The positive impact of dietary phytochemicals on overall health and longevity has been studied extensively over the past decade. The emerging role of phytochemicals as an effec...
Article
Full-text available
Apoptosis is an active response of cells to altered microenvironments which is characterized by cell shrinkage, chromatin condensation and DNA fragmentation, in variety of cell types such as renal epithelial cells, endothelial cells, mesangial cells and podocytes. Hyperglycemia is among the micro environmental factors that may facilitate apoptosis...
Article
Objectives: Parkinson disease (PD) is a neurodegenerative disorder affecting mainly the motor system, as a result of death of dopaminergic neurons in the substantia nigra pars compacta. The present scenario of research in PD is directed to identify novel molecules that can be administered individually or co-administered with L-Dopa to prevent the...
Article
Full-text available
Parkinson’s disease is a progressive neurodegenerative movement disorder with the cardinal symptoms of bradykinesia, resting tremor, rigidity, and postural instability, which lead to abnormal movements and lack of activity which in turn cause muscular damage. Even though studies have been carried out to elucidate the causative factors that lead to...
Article
Full-text available
Background: Berberis vulgaris is the most widely used drug in Homoeopathy for treating urolithiasis. However, its mechanism of action in alleviating its consequences remains uncertain. Objective: To explicate the potential role of Homoeopathic preparation of B. vulgaris on in vitro Calcium oxalate (CaOx) crystallization. Materials and Methods: Spec...
Article
A simple, accurate and rapid high performance thin layer chromatography (HPTLC)-densitometry method was developed for separation and determination of chlorthalidone (CHL) and irbesartan (IBS) in pharmaceutical dosage forms. The compounds were separated on silica gel 60 GF254, HPTLC plates using mobile phase of toluene: ethyl acetate: acetonitrile:...
Article
A reliable simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for estimation of lafutidine (LF) and domperidone (DP) in combined tablet dosage forms. The analysis was performed on 10×20 cm aluminium-precoated plates coated with 0.2 mm layers of silica gel 60 F 254 (E-Merck, German...
Article
Scope: Increased fat consumption in industrialized countries has resulted in hepatic steatosis that upregulates atherogenic aspirant genes, leading to atherosclerosis and mortality. Although extensive studies have been carried out to elucidate the atheroprotective efficacy of epigallocatechin-3-gallate (EGCG), the effect of EGCG on hepatic steatosi...
Article
Hesperidin (HDN), a polyphenolic flavonoid, had drawn attention due to its free radical scavenging and antioxidant activity. Valproate (VPA) is one of the routinely used antiepileptic drugs that has also been associated with hepatotoxicity and its metabolites, like 4-ene VPA and 2,4-diene. VPA are the main contributory compounds in inducing hepatic...
Article
Full-text available
Advanced age significantly increases cholesterol levels, however, when combined with a high cholesterol diet it not only leads to life-threatening conditions like atherosclerosis, but also plays a central role in the pathogenesis of hepatic damage and its complications. Even though extensive studies have been carried out to elucidate the causative...
Article
Full-text available
Background: Oxidative stress is a major mediator in the pathophysiology of several kidney diseases. The cellular damage is mediated by an alteration in the antioxidant status, which increases the concentration of reactive oxygen species (ROS) in the stationary state (oxidative stress). Therefore, interventions favoring the scavenging and/or depura...
Article
The research work intended to develop an accurate, simple, precise, sensitive and economical method for the estimation of Lafutidine (LF) and Domperidone (DP) in tablet dosage form by UV spectrophotometric method. UV spectrophotometric method includes simultaneous equations method (Method I) and Absorbance ratio method (Method II). For development...
Article
The study reveals a growing desire of lower level employees in the Arvind Remedies Ltd, to participate in the process of decision making. The first section of the report is concerned with workers' participation in the improvement process and in the decision making in the lower level in general. This section also gives a detailed company profile. It...
Article
The study focuses on the anti-urolithiasis potential of ultra-diluted homeopathic potency of Berberis vulgaris (B. vulgaris) root bark, commonly used in homeopathic system to treat renal calculi. B. vulgaris root bark (200c, 20 μl/100 g body weight/day, p.o, for 28 days) was tested in an animal model of urolithiasis. Urolithiasis was induced in mal...
Article
Epigallocatechin-3-gallate (EGCG), a principal polyphenol, which is most abundant and active component in green tea which has wide therapeutic effects against various diseases. However, EGCG used in clinical application has some downsides due to its low bioavailability and half-life. Hence increasing the bioavailability of EGCG without losing its p...
Article
Full-text available
Oxidative stress is a major player in aging and neurodegenerative disorders. Macromolecular damage occurs as a result of oxidative stress that affects the mitochondria. Mitochondrial damage leads to cell death by apoptosis or necrosis. EGCG is a tea polyphenol that protects the cells against oxidative stress. Neuroprotective potential of EGCG was t...
Article
Oxidative stress is recognized as a key element responsible for the development of age-related pathologies. A declining endogenous defence system during senescence dictates the need for supplementation with exogenous antioxidants through diet. Hesperidin is a naturally occurring flavonone present in citrus fruits and has been shown to have many bio...
Article
A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed as per ICH nomination for the simultaneous estimation of Hydrochlorothiazide, Amlodipine besylate and Telmisartan in tablet dosage form. The separation method was carried out by using a mobile phase consisting of 0.02M sod...
Article
Neurotransmission plays an important role in communication of messages in brain. Cholinergic alterations during aging are associated with learning and memory. Neurotransmitters and enzymes that influence these neurotransmitters are significant in age-associated memory. Neurotransmitters like acetylcholine, serotonin and dopamine levels were studied...
Article
The research work deals with the ethanolic extract of Clerodendrum philippinum schauer flowers for anti anxiety and central nervous system depressant activity. The C.philippinum flowers are known to contain sterols, flavonoid, glycosides and phenyl propanoid derivatives as chemical components and are reported to have antifungal activity. It is used...
Article
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Ethylacetate extract of the bark of evodia lunu ankenda were evaluated for analgesic activity using Eddys Hot plate method and heat conduction method on swiss albino mices. The findings support the use of this drug. evodia.lunuankenda in the treatment of painboth the extracts were not toxic up to 3000mg/kg body weight.
Article
Epidemiological studies suggest that even in the absence of other risk factors advanced age itself significantly increases cardiovascular morbidity. Age aggravated inflammatory activity further plays a central role in the pathogenesis of atherosclerosis and its complications. EGCG, a major flavonoid present in green tea extract has been proved to b...
Article
Aging is a multi-factorial process which involves deprivation in body's metabolism. Brain mitochondria are prone to oxidative damage owing to their high metabolic rate. The decline in antioxidant system during aging augments the neuronal damage to mitochondrial components like antioxidant system, Kreb's cycle enzymes and electron transport chain co...
Article
A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed as per ICH nomination for the simultaneous estimation of ofloxacin and nitazoxanide from pharmaceutical formulation. The method was carried out on a column - INERTSIL ODS (250mm x 4.6mm i.d., 5mc) with a mobile phase consi...
Article
The activities and capacities of antioxidant systems of tissue cells are declined during aging, leading to the gradual loss of pro-oxidant/antioxidant balance and accumulation of oxidative damage. Hence, the present study evaluated the role of green tea extract (GTE), rich in polyphenols, in combating age-associated macromolecular damage in rat car...
Article
Full-text available
Aging is a complex biological phenomenon which involves free radicals and oxidative stress. Brain is more susceptible and vulnerable to oxidative damage due to its high-polyunsaturated fatty acid content and high rate of aerobic metabolism. Since the antioxidant defense system is diminished during aging, antioxidant supplementation might be a prote...
Article
Ageing is defined as the loss of ability to maintain cellular homeostasis of an organism associated with the free radical-elicited oxidative damage to cellular macromolecules. The aim of this study was to evaluate the efficacy of epigallocatechin-3-gallate (EGCG), the key component of green tea catechins on attenuating the age associated oxidative...
Article
This study was done to investigate the DNA binding ability of a diagnostic biomarker, 45-kDa calcium oxalate monohydrate (COM) binding protein, isolated from human kidney and its effect on transcription. The 45-kDa COM binding protein was isolated and purified from human kidney. The subcellular localization of the protein and the amino acid composi...
Article
The pathophysiological link between increased blood concentrations of factors responsible for the derangement and erythrocyte membrane functions in chronic renal failure (CRF) patients are not thoroughly elucidated. We studied the erythrocyte characteristics and phospholipid asymmetry loss in CRF patients with different grades of uremia and also ex...
Article
The rat kidney H1 oxalate binding protein was isolated and purified. Oxalate binds exclusively with H1B fraction of H1 histone. Oxalate binding activity is inhibited by lysine group modifiers such as 4',4'-diisothiostilbene-2,2-disulfonic acid (DIDS) and pyridoxal phosphate and reduced in presence of ATP and ADP. RNA has no effect on oxalate bindin...
Article
The generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in hyperoxaluric condition has been proved experimentally. This may result in the formation of the cytotoxic metabolite peroxynitrite, which is capable of causing lipid peroxidation and protein modification. The presence of nitrotyrosine in proteins has been associa...
Article
Association of macromolecules particularly the role of proteins in urolithiasis has been studied for last few centuries, but still a complete profile of stone matrix proteins that mediate co-precipitation of uric acid and calcium oxalate has not been characterized. We isolated and characterize proteins from uric acid rich stone matrix, which have o...
Article
Full-text available
Oral supplementation of l-arginine (l-arg) is found to be beneficial in many kidney disorders. We determined whether l-arg supplementation safeguards the renal epithelial cell damage induced by hyperoxaluria with excretion of urinary marker enzymes and lithogenic salts with special reference to Tamm-Horsfall glycoprotein (THP). Hyperoxaluria was in...
Article
Full-text available
Nitrosative stress plays a role in calcium oxalate stone formation, as nitrosated proteins have been identified in stone formers. Nitric oxide (NO(*)), the common precursor for reactive nitrogen species, is synthesized in the juxtaglomerular apparatus of the kidneys. The present study is aimed to determine the role of nitric oxide synthase (NOS) in...
Article
Full-text available
This study aimed to assess the therapeutic efficacy of oral vitamin E supplementation on the biochemical and kinetic properties of Tamm-Horsfall glycoprotein (THP) in hypertensive and hyperoxaluric patients. Newly detected hypertensives (n = 200) and stone formers (n = 200) were each subdivided into two groups. One group (n = 100) was administered...
Article
Full-text available
Our understanding of nitrosative stress in the process of urolithiasis is far from complete. Earlier studies carried out in our laboratory demonstrate the presence of nitrated THP in stone formers, l-arginine (l-arg) a precursor of nitric oxide (NO), attenuates the endothelial dysfunction caused by reactive nitrogen species. We investigated the rol...
Article
Full-text available
Free radical induced renal damage leads to crystal retention and formation of large stones. However, the scenario behind uric acid (UA) stone formation is still a mystery, as uric acid, a risk factor of stone formation, seems to be a potent antioxidant that can protect cells from damage by reactive oxygen species. This study was intended to evaluat...
Article
Full-text available
Adriamycin (ADR), a cytotoxic antineoplastic drug, is used in the treatment of various solid tumors. However, its efficacy continues to be challenged by significant toxicities including nephrotoxicity. In the present study, the effects of N-acetyl cysteine (NAC) and vitamin E, known antioxidants, were investigated on ADR-induced peroxidative damage...
Article
Full-text available
The crucial steps involved in the lithogenic process are governed by the macromolecular components of urine, of which proteins play a major role. Structurally abnormal proteins have been reported to be present in the urine of stone formers. Free radical injury has come a long way in explaining some of the pathophysiological events of renal lithiasi...
Article
Full-text available
Previous studies have shown that urogenital tuberculosis (GuTb) patients treated or untreated with regular anti-Tb regimen excrete comparatively high levels of urinary stone forming constituents than normal subjects. Enhanced oxidative stress is also considered as a prime factor that accelerates urolithiasis. The present study was aimed to determin...
Article
Urinary oxalate is a biomarker for calcium oxalate kidney stone disease; however, its assay is insensitive and nonspecific. Calcium oxalate monohydrate (COM) binding protein (45 kDa) is a promoter of calcium oxalate kidney disease, which is markedly upregulated by oxalate induced oxidative stress. The current study was carried out to evaluate wheth...
Article
C-phycocyanin, a biliprotein pigment found in some blue green algae (Spirulina platensis) with nutritional and medicinal properties, was investigated for its efficacy on sodium oxalate-induced nephrotoxicity in experimentally induced urolithic rats. Male Wistar rats were divided into four groups. Hyperoxaluria was induced in two of these groups by...
Article
Urolithiasis is a multifactorial process that starts with the formation of microcrystals in the urine and terminates as mature renal calculi. The oxalate binding protein plays a vital role in the transport of oxalate. The physiological significance of the presence of oxalate binding protein in the nuclear pore complex is not well understood. The nu...
Article
Oxalate induced renal calculi formation and the associated renal injury is thought to be caused by free radical mediated mechanisms. An in vivo model was used to investigate the effect of phycocyanin (from Spirulina platensis), a known antioxidant, against calcium oxalate urolithiasis. Male Wistar rats were divided into four groups. Hyperoxaluria w...