Pelin Sağlam Metiner

• Doctor of Engineering
• PostDoc at Ege University

Propofol and midazolam are the current standard of care for prolonged sedation in Intensive Care Units (ICUs). However, the effects and mechanism of these sedatives in brain tissue are unclear. Herein, the development of an ICU patient-on-a-chip platform to elucidate those effects is reported. The humanized neural tissue compartment combines mast c...

Background and Objective The updated World Health Organization (WHO) air quality guideline recommends an annual mean concentration of fine particulate matter (PM2.5) not exceeding 5 or 15 μg/m³ in the short‐term (24 h) for no more than 3–4 days annually. However, more than 90% of the global population is currently exposed to daily concentrations su...

Background and objective Exposure to inorganic dust and particulate matter (PM) disrupts the integrity and function of the airway epithelial barrier (AEB). Herein, the immune response and epithelial barrier integrity disruption effect of respirable ex vivo airway tissue models. Methods Particles at an average size of 1 µm, referred to as ex vivo. C...

Breast cancer progression is a complex process involving the primary tumor and its microenvironment supported by a vascular network. Multicellular spheroids closely mimic in vivo-like tumor conditions, paving the way for effective treatments. Although spheroid formation by simulated microgravity emerges as a promising technique for cancer spheroids...

The complex structure and function of the human central nervous system that develops from the neural tube made in vitro modeling quite challenging until the discovery of brain organoids. Human-induced pluripotent stem cells–derived brain organoids offer recapitulation of the features of early human neurodevelopment in vitro, including the generatio...

Advances in stem cell (SC) technology allow the generation of cellular models that recapitulate the histological, molecular and physiological properties of humanized in vitro three dimensional (3D) models, as well as production of cell-derived therapeutics such as extracellular vesicles (EVs). Improvements in organ-on-chip platforms and human induc...

Rett syndrome (RTT) is a rare genetic neurodevelopmental disorder that has no cure apart from symptomatic treatments. While intense research efforts are required to fulfill this unmet need, the fundamental challenge is to obtain sufficient patient data. In this study, we used human transcriptomic data of four different sample types from RTT patient...

The bioengineerined and whole matured human brain organoids stand as highly valuable three-dimensional in vitro brain-mimetic models to recapitulate in vivo brain development, neurodevelopmental and neurodegenerative diseases. Various instructive signals affecting multiple biological processes including morphogenesis, developmental stages, cell fat...

Resistance to currently available antifungal agents raises the need to develop alternative remedies. Candida albicans is the most common opportunistic pathogenic fungus of humans, colonizing in the genital and intestinal mucosa, skin, and oral-nasal cavity and reducing quality of life. Herein, essential oil from grapefruit (Citrus paradise) peels w...

Green technology is the creation and usage of products, equipment, and systems that help to protect the natural environment and resources by reducing and/or minimizing the negative effects of human activity within the concept of sustainability. In recent years, supercritical fluids have been of interest in green biotechnological processes. Although...

Animal cell culture scale-up technology in today's scenario has become indispensable in the field of life sciences to profit from time, space and cost. In addition to its use as a tool for in vivo xenegroft models, it provides production of kinds of FDA approved enzymes, vaccines, anticancer agents, immunobiologicals and hormons [1]. Industrial-sca...

Astaxanthin is one of the most attractive carotenoid in the cosmetic, food, pharmaceutical, and aquaculture industries due to its strong bioactive properties. Among the various sources, several algae species are considered as rich sources of astaxanthin. Downstream processing of algae involves the majority of the total processing costs. Thus, elimi...

The temporomandibular joint (TMJ) is commonly affected during fundamental oral activities, reducing the quality of life. Herein, we synthesized a heparin‐conjugated poloxamer hydrogel (HEP) as a thermo‐responsive injectable hydrogel for the treatment of TMJ disorders. While the gelation temperature of synthesized HEP (25% [w/v]) was 29.8–30.0°C, th...

The immune response after implantation of a biomaterial may shorten the functional life of the implant, depending on the degree of the response. In this study, we used a polyacrylamide-alginate (PAAm-Alg) hydrogel, which has been previously characterized as a biocompatible material and shown to enhance regeneration of cartilage in vivo, along with...

Breast cancer was ranked first in global cancer incidence in 2020, and HER2 overexpression in breast cancer accounts for 20–30% of breast cancer patients. Current therapeutic strategies increase the survival rate, but resistance to them occurs frequently, and there is an urgent need to develop novel treatments such as DNA vaccines which can induce...

Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg)-polyacrylamide (PAAm) with and without the addition of TGF-β3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments. Exceeding the mechanical strength of...

Fabrication of immunocompatible tissue constructs for bone-cartilage defect regeneration is of prime importance. In this study, a double layer hydrogel was successfully synthesized, where alginate/polyacrylamide were formulated to represent cartilage layer (5-10% (w/w) total polymer ratio) and laponite XLS (2-5-8% (w/w))/alginate/polyacrylamide for...

Topical formulations containing 1–2% of Pinus brutia bark extract and Pycnogenol® have been prepared to investigate the effect of flavonoids on the stability of O/W emulsions, which were subjected to physicochemical and thermal stability tests. The formulations have been applied to cotton fabrics to evaluate antimicrobial properties against Staphyl...

The cell and matrix content in the central nervous system (CNS) regulates a number of fundamental neural processes and decellularized brain matrices have drawn attention for biomedical applications. However, the effect of sterilization methods on the structural/mechanical stability and cell/matrix contents have yet to be addressed. In this study, s...

Three-dimensional (3D) spheroid cell cultures are excellent models used in cancer biology research and drug screening. The objective of this study was to develop a lung carcinoma spheroid based microfluidic platform with perfusion function to mimic lung cancer pathology and investigate the effect of a potential drug molecule, panaxatriol. Spheroids...

Current implantable materials are limited in terms of function as native tissue, and there is still no effective clinical treatment to restore articular impairments. Hereby, a functionalized polyacrylamide (PAAm)-alginate (Alg) Double Network (DN) hydrogel acting as an articular-like tissue is developed. These hydrogels sustain their mechanical sta...

The central nervous system (CNS), consisting of the brain and spinal cord, regulates the mind and functions of the organs. CNS diseases, leading to changes in neurological functions in corresponding sites and causing long-term disability, represent one of the major public health issues with significant clinical and economic burdens worldwide. In pa...

In this study, the neoplastic drug frequently used in the treatment of lung cancer, carboplatin is loaded to microbubbles via a microfluidic platform. In order to increase the drug loading capacity of microbubbles, carboplatin is encapsulated into alginate polymer layer. The phospholipid microbubbles (MBs) are synthesized by MicroSphere Creator, wh...

BACKGROUND Propolis exhibits therapeutic properties due to the presence of phenolic acids, esters, and flavonoids. The scope of this study was to develop a nano‐vesicular formulation and establish a three‐dimensional (3D) spheroid model in which lung cancer is recapitulated. RESULTS Niosome vesicles doped with galangin‐rich propolis extract were s...

Background Serum have been traditionally used to support growth of animal cell cultures. However, the increasing growth of therapeutic biopharmaceuticals market, accelerated the high demand for the serum-free medium (SFM). Objective The main objective is to design a SFM for a stable rCHO cell line that produces a fully anti-human TNF-α monoclonal...

Breast cancer is one of the most common cancer types among women in which early tumor invasion leads to metastases and death. EpCAM (epithelial cellular adhesion molecule) and HER2 (human epidermal growth factor receptor 2) are two main circulating tumor cell (CTC) subsets in HER2+ breast cancer patients. In this regard, the main aim of this study...

The potential use of stem cells in the field of cell therapy and regenerative medicine has accelerated with the development of new culture technologies and large-scale production for surface-dependent cells. Microcarrier technology in spinner flask systems are very suitable for laboratory research. The microcarriers used allow the growth of surface...

Toxoplasma gondii can infect nearly all warm-blooded animals, including humans. In the laboratory diagnosis of toxoplasmosis, serological tests have importance in detecting antibody response. Traditionally T. gondii tachyzoites grown in vivo are being used as an antigen source in serological assays. Currently, tachyzoites produced in vitro are bein...

İki boyutlu çalışmaların karmaşık bitkisel formlar hakkında yetersiz kalması ve gelişen teknolojilerin daha ayrıntılı veriler toplanmasına izin vermesi, araştırmacıları yeni alternatifler arama yoluna itmiştir. Bitki biyoteknolojisi ile ilgili gelişmeler, son yirmi yılda mikroskobi, yapısal ve fonksiyonel bitki modellemeleri, fenotiplendirme ve gen...

Objective: KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid), a member of the sialic acid family, is a hapten-structured low-molecular-weight monosaccharide on the cell membrane, which cannot induce immune responses without a carrier protein. Since it is over-expressed on the cancerous cells’ membrane, it is thought to be a great target molecul...

In the scope of the study, Montanide ISA 61 VG, Montanide ISA 201 VG and Montanide IMS 1313 VG NPR adjuvants were used as KDN carriers compared to Freund's adjuvant. Immunization was performed at 8 different times in total with 4 Balb/c mice for each adjuvant groups and control group without any immunization. Indirect ELISA was applied to the serum...

Nowadays the genotoxic effects of commonly used cosmetic ingredients are unknown. In this study, we used two different raw cosmetic material; ıvy leaf extract and grape seed extract which supplied from BIONORM Natural Products Limited Company for the micronucleus test as a genotoxic test. Two different methods that is surface dependent cell and sus...

“Her aşı tipi için antijenin özelliğine, aşı hazırlama sürecinin türüne bağlı olarak, her üretim basamağı için belirlenmiş farklı temizoda standartları mevcuttur. Sonuçta hedef; ürünü, etkin kontrol aygıtlarının da katılımı ile olası kontaminasyonlardan korumaya yönelik tasarlanmış bir ortam oluşturulmasıdır.”

Recent developments in medical biotechnology have facilitated to enhance the production of monoclonal antibodies (mAbs) and recombinant proteins in mammalian cells. Human mAbs for clinical applications have focused on three areas, particularly cancer, immunological disorders, and infectious diseases. Tumor necrosis factor alpha (TNF-α), which has b...