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Introduction
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September 1990 - October 1992
January 1983 - present
Publications
Publications (368)
La microbiota varía con el tiempo, por ello durante el curso académico 2019-20, coincidiendo con la pandemia SARS-COV-2, se evaluó la evolución de la misma en un aula de la Facultad de Farmacia de la Universidad de Salamanca con la espectrometría de masas Matrix-assisted laser desorption ionization time-of-flight. Además, se comparó con otros estud...
Homologs of qseG gene (coding for the membrane protein QseG), along with the qseEF genes, are present in many Enterobacteriaceae; however, its role in non-pathogenic strains is still unknown. To fill this knowledge gap, we investigated the role of QseG protein of a plant-associated enterobacterium in the interactions with its legume host and in the...
Canola (Brassica napus L. var. oleracea) is the third most common oil-producing crop worldwide after palm and soybean. Canola cultivation requires the use of chemical fertilizers, but the amount required can be reduced by applying plant growth-promoting bacteria (PGPB). Among PGPB, endophytic bacteria have certain advantages as biofertilizers, but...
Microbial inoculants are gaining prominence in technologically advanced agri-systems due to the need for alternatives to the most pollutant agricultural inputs. The objective of this work was to improve the agronomic performance of the rhizobial inoculants for common bean, based on the superior native strain Rlp-LCS0306 of Rhizobium leguminosarum b...
Rapeseed (Brassica napus L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative effects on human health and the environment. Plant growth-promoting bacteria may be used to re...
El consumo indebido de antibióticos ha incrementado el número de personas que enferman y mueren por infecciones provocadas por microorganismos resistentes a estos antimicrobianos. El objetivo de este estudio fue aislar bacterias rizosféricas y evaluar su potencial para producir nuevos compuestos antimicrobianos. Se aislaron 418 colonias y se enfren...
The emergence of antimicrobial resistance in pathogenic agents has raised awareness among society and nowadays is a recognized threat to public health. This problem is aggravated due to the misuse of current antibiotics and the lack of novel antimicrobial compounds 1. Soil microorganisms are a potential source of new antibiotics and, thanks to the...
Primary infection of legumes by rhizobia involves the controlled localized enzymatic breakdown of cell walls at root hair tips. Previous studies determined the role of rhizobial CelC2 cellulase in different steps of the symbiotic interaction Rhizobium leguminosarum-Trifolium repens. Recent findings also showed that CelC2 influences early signalling...
Plants harbor a diversity of microorganisms constituting the plant microbiome. Many bioinoculants for agricultural crops have been isolated from plants. Nevertheless, plants are an underexplored niche for the isolation of microorganisms with other biotechnological applications. As a part of a collection of canola endophytes, we isolated strain CDVB...
Melinis minutiflora is an invasive species that threatens the biodiversity of the endemic vegetation of the campo rupestre biome in Brazil, displacing the native vegetation and favouring fire spread. As M. minutiflora invasion has been associated with a high nitrogen (N) demand, we assessed changes in N cycle under four treatments: two treatments w...
Aims
The aim of this study was to investigate whether the overexpression of NifA and NodD regulators contribute to the symbiotic improvement of chickpea mesorhizobia.
Methods
The native strains V-15b, ST-2, and PMI-6 were transformed with extra copies of nifA or nodD genes and several plants trial were performed.
Results
Plant growth assays showe...
Phaseolus vulgaris and Lens culinaris are two legumes with different distribution centers that were introduced in Spain at different times, but in some regions L. culinaris has been traditionally cultivated and P. vulgaris did not. Here we analysed the rhizobia isolated from nodules of these two legumes in one of these regions. MALDI-TOF MS analysi...
The infection of legume plants by rhizobia is tightly regulated to ensure accurate bacterial penetration, infection and development of functionally efficient nitrogen-fixing root nodules. Rhizobial Nod Factors (NF) have key roles in the elicitation of nodulation signalling. Infection of white clover roots also involves the tightly regulated specifi...
The growing interest in a healthy lifestyle and in environmental protection is changing habits regarding food consumption and agricultural practices. Good agricultural practice is indispensable, particularly for raw vegetables, and can include the use of plant probiotic bacteria for the purpose of biofertilization. In this work we analysed the prob...
Trifolium rubens L. is a leguminous plant “Preferential Attention”, according to the Catalog of Protected Flora of Castile and Leon (Spain). In this study we aimed to analyze the potential of three bacterial strains of the genus Rhizobium to improve the growth and development of this plant. All three strains produced 3-indoleacetic acid (IAA), but...
In this study, three strains belonging to the genus Mesorhizobium, CSLC115NT, CSLC19N and CSLC37N, isolated from Lotus corniculatus nodules in Spain, were characterized. Their 16S rRNA gene sequences were closely related to those of Mesorhizobium metallidurans STM 2683T, Mesorhizobium tianshanense A-1BST, Mesorhizobium tarimense CCBAU 83306T, Mesor...
The mutualistic interactions between nodule-forming rhizobia and specific legume host plants involve a series of signalling molecules leading to the establishment of a strong and functional symbiosis between the two partners. The competitive ability and legume host specificity of rhizobia together with the ability of both rhizobia and legumes to re...
Throughout history, the evolution and progress of all human civilizations have been closely linked to the evolution and development of agriculture, since this is the basis of food production to sustain population and ensure social stability.
At the beginning of the twentieth century, due to great advances in medicine, world population increased sig...
Small non-coding RNAs (sRNAs) are expected to have pivotal roles in the adaptive responses underlying symbiosis of nitrogen-fixing rhizobia with legumes. Here, we provide primary insights into the function and activity mechanism of the Sinorhizobium meliloti trans-sRNA NfeR1 (Nodule Formation Efficiency RNA). Northern blot probing and transcription...
A bacterial strain designated RA9T was isolated from a root of Cistus ladanifer in Spain. Phylogenetic analyses based on 16S rRNA gene sequence placed the isolate into the genus Bacillus with its closest relatives being B. fortis R-6514T and B. fordii R-7190T with 98.2% similarity in both cases. DNA-DNA hybridization studies showed averages of 29 a...
The Serra do Rola Moça State Park (PESRM) in Minas Gerais State, Brazil is a preserved site representative of the campo rupestre biome over an ironstone outcrop that has a high level of plant diversity. Almost 60% of this grassy field has been invaded by the exotic molasses grass (Melinis minutiflora), which constitutes a severe threat to the biodi...
Rhizobium leguminosarum bv. trifolii CelC2 cellulase is a 1,4-β-d-endoglucanase involved in the hydrolysis and biosynthesis of rhizobial cellulose. These special features make this enzyme essential in the plant infection process and important in root surface colonization and biofilm formation. A loss-of-function of the celC gene causes an increase...
Plant growth-promoting rhizobacteria (PGPR) are one of the most important groups of rhizospheric microorganisms, such as free-living bacteria, endophytes, and endosymbiotic bacteria. PGPRs colonize plant roots, conferring several benefits to the plants through different mechanisms. Despite its classical involvement in root nodule symbiosis with leg...
Plant growth-promoting rhizobacteria are a group of bacteria able to promote plant growth and increase crop productivity. For a successful and effective bacteria–plant association, microorganism colonization and its arrangement and abundance on plant root surfaces are very important. Here, we analysed the ability of the strain PEPV12 identified as...
Bacterial endophytes
live inside plants for at least part of their life cycle, because plants are very attractive as nutrient reservoirs for such bacteria. Plants need the presence of those associated bacteria for their growth and adaptation to different ecosystems. This is the case of maize, which is a well-known cereal crop for hosting a great di...
A bacterial strain designated PM10T was isolated from root nodules of Periandra mediterranea in Brazil. Phylogenetic analyses based on 16S rRNA gene sequence placed the isolate in the genus Paenibacillus being its closest relatives P. vulneris and P. yunnanensis with 95.6 and 95.9 similarity, respectively. The isolate was a Gram-variable, motile an...
The ClpB chaperone is known to be involved in bacterial stress response. Moreover, recent studies suggest that this protein has also a role in the chickpea-rhizobia symbiosis. In order to improve both stress tolerance and symbiotic performance of a chickpea microsymbiont, the Mesorhizobium mediterraneum UPM-Ca36T strain was genetically transformed...
Qualitative assays of cellulose (A) and cellulase (B) production. Cellulose production was detected using Congo Red staining, and CMCase activity by double-layer plate assays after inoculation with 10 μL of each strain. 1. R. rhizogenes ATCC11325T, 2. R. cellulosilyticum ALA10B2T, 3. R. leguminosarum bv phaseoli TPV08, 4. R. pisi DSM30132T, 5. R. j...
The diversity of rhizobia that establish symbiosis with Lotus corniculatus has scarcely been studied. Several species of Mesorhizobium are endosymbionts of this legume, including Mesorhizobium loti, the type species of this genus. We analysed the genetic diversity of strains nodulating Lotus corniculatus in Northwest Spain and ten different RAPD pa...
The Rhizobium-legume symbiosis is a complex partnership with many factors, with initial bacterial colonization of the plant root surface and primary infection as key early stages. Two molecules are strongly involved in these processes: the structural carbohydrate cellulose and the enzyme cellulase, which breaks down the former and allows rhizobia t...
There is increasing interest in the use of plant growth-promoting rhizobacteria (PGPR) as environmental-friendly and healthy biofertilizers. Strawberries (Fragraria x ananassa) are mainly consumed fresh and hence any PGPRs used for biofertilization must be safe for humans, which is the case for members of the genus Rhizobium. In this study, the eff...
Xy.la.ni.mo'nas. N.L. n. xylanum xylan a polysaccharide; Gr. fem. n. monas a monad, unit; N.L. fem. n. Xylanimonas a monad from xylan.
Actinobacteria / Actinobacteria / Micrococcales / Promicromonosporaceae / Xylanimonas
Gram‐stain‐positive, non‐spore forming coccoid cells . Aerial mycelium is not formed. Aerobic or facultatively anaerobic , chemo‐...
Rhizobia grow as free-living organisms but can also induce and colonize root nodules in legume plants thereby establishing a partnership that benefits both organisms. The establishment of rhizobia as nitrogen-fixing endosymbionts within legume root nodules requires plant root colonization and the disruption of the plant cell wall to breach the host...
We isolated a strain coded Esc2AmT during a study focused on the microbial diversity of adult specimens of the bark beetle Hylesinus fraxini. Its 16S rRNA gene sequence had 99.4% similarity with respect to its closest relative Pseudomonas rhizosphaerae IH5T. The analysis of partial housekeeping genes rpoB, rpoD and gyrB genes confirmed that strain...
Cicer canariense is a threatened endemic legume from the Canary Islands where it can be nodulated by mesorhizobial strains from the symbiovar ciceri, which is the common worldwide endosymbiont of Cicer arietinum linked to the genus Mesorhizobium. However, when C. canariense was cultivated in a soil from mainland Spain, where the symbiovar ciceri is...
The increasing interest in the preservation of the environment and the health of consumers is changing production methods and food consumption habits. Functional foods are increasingly demanded by consumers because they contain bioactive compounds involved in health protection. In this sense biofertilization using plant probiotics is a reliable alt...
The species Mesorhizobim loti was isolated from nodules of Lotus corniculatus and its type strain deposited in several collections. Some of these type strains are not coincident with the original strain deposited in NZP culture collection, NZP 2213T, such as those deposited in USDA and ATCC collections before 1990. The analysis of the 16S rRNA gene...
The species Rhizobium lupini was isolated from Lupinus nodules and included in the Approved Lists of Bacterial Names in 1980. Nevertheless, on the basis of the analysis of the type strain of this species available in DSMZ, DSM 30140T, whose 16S rRNA gene was identical to that of the type strain of Bradyrhizobium japonicum, R. lupini was considered...
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Microbial biofertilizers are becoming an effective tool for sustainable agriculture by means of the reduction of the use of chemical fertilizers. However, the knowledge of each specific plant–microorganism interaction is essential for a correct application. In this study, we analyzed the in vitro plant-growth-promotion mechanisms of a Rhizobium leg...
