Pedro L. Granja

Pedro L. Granja
University of Porto | UP · Instituo de Investigação e Inovação em Saúde (i3S)

PhD

About

176
Publications
61,117
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
6,716
Citations
Citations since 2016
81 Research Items
4743 Citations
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
Introduction
PL Granja graduated in Metallurgical Engineering in 1993 and obtained his PhD in 2001 both from U. Porto. He is Group Leader at i3S - Institute for Research and Innovation of U. Porto. His research interests lie on the development of biomaterials for tissue engineering and regenerative medicine. His main focus is on biofabrication using molecularly designed biofunctional hydrogels.
Additional affiliations
June 1994 - March 2016
University of Porto
Position
  • Scientific Coordinator
Description
  • Institute for Biomedical Engineering

Publications

Publications (176)
Article
Full-text available
Colorectal cancer (CRC) has been addressed in the framework of molecular, cellular biology, and biochemical traits. A new approach to studying CRC is focused on the relationship between biochemical pathways and biophysical cues, which may contribute to disease understanding and therapy development. Herein, we investigated the mechanical properties...
Article
Full-text available
Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation rate is not the most adequate for specific applicat...
Article
Full-text available
In this review, the mechanobiology of colorectal cancer (CRC) are discussed. Mechanotransduction of CRC is addressed considering the relationship of several biophysical cues and biochemical pathways. Mechanobiology is focused on considering how it may influence epithelial cells in terms of motility, morphometric changes, intravasation, circulation,...
Chapter
Wound management challenges everyday thousands of health professionals, mainly due to the constant monitoring and difficulties in deciding the correct treatment options. When considering chronic wounds, selecting the ideal dressing defies clinical knowledge, when facing the large amount of different materials, its distinctive properties and the uni...
Article
Full-text available
Organ-on-a-chip technology promises to revolutionize how pre-clinical human trials are conducted. Engineering an in vitro environment that mimics the functionality and architecture of human physiology is essential toward building better platforms for drug development and personalized medicine. However, the complex nature of these devices requires s...
Article
Full-text available
In situ cross-linked hydrogels have the advantage of effectively fulfilling the wound in its shape and depth. Amongst the new generation of natural-based biopolymers being proposed for wound care and skin regeneration, silk sericin is particularly interesting due to its exceptional properties such as biocompatibility, biodegradability, and antioxid...
Article
Full-text available
Objective Arthropathy is a major clinical problem in patients with hemochromatosis, the most common genetic disorder of iron overload. The pathological features of hemochromatosis arthropathy (HA) are heterogeneous and its specific nature remains unknown. One important drawback is the lack of proper in vitro models. The aim of the present study was...
Article
Full-text available
Gastric cancer (GC) remains a major cause of death worldwide mainly because of the late detection in advanced stage. Recently, we proposed CD44v6 as a relevant marker for early detection of GC, opening new avenues for GC-targeted theranostics. Here, we designed a modular nanoscale system that selectively targets CD44v6-expressing GC cells by the si...
Article
Despite its complexity, the human body is composed of only four basic tissue types, namely epithelial, connective, muscular and nervous tissues. Notably, each tissue is an assemblage of similarly functional cells united in performing a specific function. Instead of mimicking functionality mechanically, three-dimensional (3D) bioprinting based on hi...
Article
This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchyma...
Article
Full-text available
The properties of the surrounding cell environment are major determinants of cell response in 3D. However, the ability to unravel how these cues dictate the biological function in bioprinted constructs is limited by the lack of extracellular matrix (ECM)‐mimetic bioinks with fully controllable properties. In this study, a multifunctional bioink tha...
Article
Interactions between cell types, growth factors, and extracellular matrix components involved in angiogenesis are crucial for new vessel formation leading to tissue regeneration. This study investigated whether cocultures of fibroblasts and endothelial cells (ECs; from macro‐ or microvasculature) play a role in the formation of microvessel‐like str...
Article
Colorectal cancer (CRC) is the third most common and the second deadliest type of cancer worldwide, urging the development of more comprehensive models and of more efficient treatments. Although the combination of nanotechnology with chemo- and immuno-therapy has represented a promising treatment approach, its translation to the clinic has been ham...
Article
The success of diabetes treatment with bioartificial pancreas is still hindered by a high rate of graft failure caused by poorly functioning cells. The incretin effect of glucagon like peptide-1 (GLP-1) make this peptide an attractive therapeutic agent to improve the success of transplantation of encapsulated beta cells. Our objective was to develo...
Article
Full-text available
CD44v6-containing isoforms are frequently de novo expressed in gastric cancer (GC). Whether CD44v6 has a central role in GC transformation and/or progression, whether it conditions response to therapy or whether it is only a bystander marker is still not known. Therefore, we aimed to clarify the role of CD44v6 in GC. We generated GC isogenic cell l...
Article
Full-text available
Skin is the largest organ of the human body with several important functions that can be impaired by injury, genetic or chronic diseases. Among all skin diseases, melanoma is one of the most severe, which can lead to death, due to metastization. Mechanotransduction has a crucial role for motility, invasion, adhesion and metastization processes, sin...
Chapter
The bioartificial pancreas presents a promising methodology to treat diabetes. However, this approach is still associated with a high rate of graft failure caused by poorly functioning cells. The incretin effect of glucagon like peptide-1 (GLP-1) make this peptide an attractive therapeutic agent to improve the success of transplantation of encapsul...
Article
Implantable medical devices infection and consequent failure is a severe health issue, which can result from bacterial adhesion, growth, and subsequent biofilm formation at the implantation site. Graphene-based materials, namely graphene oxide (GO), have been described as potential antibacterial agents when immobilized and exposed in polymeric matr...
Article
Full-text available
Biopolymers derived from polysaccharides are a sustainable and environmentally friendly alternative to the synthetic counterparts available in the market. Due to their distinctive properties, the cyanobacterial extracellular polymeric substances (EPS), mainly composed of heteropolysaccharides, emerge as a valid alternative to address several biotec...
Article
Hydrogels can mimic several features of the cell native microenvironment and have been widely used as synthetic extracellular matrices (ECMs) in tissue engineering and regenerative medicine (TERM). However, some applications have specifications that hydrogels cannot efficiently fulfill on their own. Incorporating reinforcing structures like fibrous...
Article
Full-text available
Introduction The success of a bioartificial pancreas crucially depends on ameliorating encapsulated beta cells survival and function. By mimicking the cellular in vivo niche, the aim of this study was to develop a novel model for beta cells encapsulation capable of establishing an appropriate microenvironment that supports interactions between cell...
Article
Full-text available
Glutaraldehyde (GTA) is a dialdehyde used as biological fixative and its interaction with proteins like bovine serum albumin (BSA) has been well described. Additionally, GTA is known to induce fluorescence when interacting with BSA molecules. In this work, it is developed a new sensitive and reproducible method for BSA quantification using GTA cros...
Article
Full-text available
Vaccination represents a promising strategy for cancer therapy due to its ability to efficiently eliminate tumors from the host body and prevent their recurrence. Nevertheless, the current vaccines are still lacking efficacy. Combination therapies, such as those integrating chemotherapy with immunotherapy, represent a powerful tool to potentially c...
Article
Cyanobacterial extracellular carbohydrate polymers are particularly attractive for biotechnological applications. Previously, we determined the monosaccharidic composition of the polymer of a Synechocystis ΔsigF overproducing mutant. Here, we further characterized this polymer, demonstrated that it is possible to recover it in high yields, and succ...
Article
In this study, silk fibroin (SF)/poly(ethylene oxide) (PEO) membranes were designed and fabricated by combining ultrasound sonication prior to electrospinning (0 to 20 min) as a strategy to physically control the rheological properties of solutions (10 to 30% w/v PEO) and to improve the spinnability of the system. PEO has proved to be essential as...
Article
Wound dressings made by electrospun nanofibers have been demonstrating great potential to regenerate skin tissue as compared to the conventional membrane products available in the market. Until today most of the developed dressings have only demonstrated the capability to regenerate the dermis or epidermis. In this study we propose new hybrid elect...
Preprint
Full-text available
Late diagnosis, modest treatment options and lack of predictive markers of therapy response dictate the poor overall survival (OS) of ∼1 year in most gastric cancer (GC) patients. We hypothesized that the level of CD44v6 expression in tumor cells could predict therapy response and prognosis in GC patients. We analyzed a surgical tumor series of GC...
Article
The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined. Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to a...
Article
Full-text available
New mesoporous silk fibroin (SF)/silica hybrids were processed via a one-pot soft and energy-efficient sol–gel chemistry and self-assembly from a silica precursor, an acidic or basic catalyst, and the ionic liquid 1-butyl-3-methylimidazolium chloride, acting as both solvent and mesoporosity-inducer. The as-prepared materials were obtained as slight...
Article
Advances on the fabrication of sintering-free biphasic calcium phosphate (BCP)/natural polymer composite scaffolds using robocasting as additive manufacturing technique are presented in the present work. Inks with high amounts of BCP powders (45 vol%) containing different HA/β-TCP ratios, in presence of crosslinked polymer, were successfully fine-t...
Article
Targeting of CD44 isoforms containing exon v6 (CD44v6) represents a viable strategy for the therapy and/or early diagnosis of metastatic cancers of the epithelium (e.g. gastric and colorectal cancer). We developed and characterized for the first time poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) modified with polyethylene glycol (P...
Article
Full-text available
Bioprinting is attractive to create cellularized constructs for skin repair. However, the vast majority of bioinks present limitations in the printing of chemically defined 3D constructs with controllable biophysical and biochemical properties. To address this challenge, a single-component hydrogel bioink with controlled density of cell-adhesive li...
Conference Paper
Full-text available
Introduction Gastric cancer (GC) is the 3rd leading cause of cancer related deaths and the 5th commonest cancer worldwide, affecting ~1 million individuals per year. For early-stage disease, surgical resection is potentially curative, however >80% of GC patients present advanced, unresectable and not curable disease, and an average overall survival...
Article
Probiotics are increasingly gaining popularity in vast food applications due to their recognized health benefits to the host. However, their passage through the gastrointestinal (GI) tract is not smooth and a significant number of physiological barriers (e.g. low pH, bile salts, enzymes, peristaltic movements, etc.) may considerably affect their vi...
Article
Gravity influences physical and biological processes, especially during development and homeostasis of several tissues in the human body. Studies under altered gravity have been receiving great attention towards a better understanding of microgravity, hypogravity (<1g) or hypergravity (>1g) induced alterations. In the present work, the influence of...
Article
Targeted drug delivery with nanoparticles (NPs) requires proper surface ligand presentation and availability. Surfactants are often used as stabilizers in the production of targeted NPs. Here, we evaluated the impact of surfactants on ligand functionalization and downstream molecular recognition. Our model system consisted of fluorescent poly(lacti...
Article
Full-text available
CD44, an abundantly expressed adhesion molecule, and its alternative splice variants have been associated with tumorigenesis and metastasis. In the context of gastric cancer (GC), de novo expression of CD44 variant 6 (CD44v6) is found in more than 60% of GCs, but its role in the pathogenesis and progression of this type of cancer remains unclear. U...
Conference Paper
Gravity influences physical and biological processes, having a recognized impact in tissue development and homeostasis. The use of simulated hypergravity has been suggested as a promising tool for cell stimulation aiming at Tissue Engineering (TE) applications. Such mechanical forces can be particularly interesting for applications over mechanores...
Article
Full-text available
Spheroid culture has gained increasing popularity, arising as a promising tool for regenerative medicine applications. Importantly, spheroids may present advantages over single-cell suspensions in cellbased therapies (CT). Unfortunately, most growth media used for spheroid culture contain animal origin-components, such as fetal bovine serum (FBS)....
Article
The combination of chemo and immunotherapy represents one promising strategy to overcome the existent challenges in the present-day anti-cancer therapy. Here, spermine-modified acetalated dextran nanoparticles (Sp-AcDEX NPs), co-loaded with the non-genotoxic molecule Nutlin-3a (Nut3a), and the cytokine granulocyte-macrophage colony-stimulating fact...
Article
Full-text available
Significance: Photopolymerizable hydrogels are attractive for skin applications due to its unique spatiotemporal control over the hydrogel formation. This study reports the design of a promising photo-clickable pectin hydrogel which biophysical and biochemical properties can be independently tailored to control cell behavior. A fast method for the...
Article
The immune system can be intelligently programmed to fight cancer. In article number 1703303 by Mohammad-Ali Shahbazi, Bruno Sarmento, Hélder A. Santos, and co-workers, spermine-modified acetalated dextran nanoparticles are co-loaded with a MDM-2 inhibitor and a cytokine for chemo-immunotherapy. Immune cell recruitment to the cancer cells and its a...
Article
The influence of microgravity and hypergravity on living systems has attracted significant attention, but the use of these tools in tissue engineering (TE) remains relatively unexplored. This Forum article highlights an emerging field of research to uncover new potential applications at the interface between altered gravity and TE.
Conference Paper
Mechanical loading like gravity is known to play a critical role during development and on the homeostasis of several tissues in the human body, particularly of the musculoskeletal system. In the particular case of tendons, these tissues act as mechanically active bridges by transmitting forces generated by muscles to bones. Thus, tendons are subje...
Article
Full-text available
Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in pre-defined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, e...
Article
Antibodies are essential in modern life sciences biotechnology. Their architecture and diversity allow for high specificity and affinity to a wide array of biochemicals. Combining monoclonal antibody (mAb) technology with recombinant DNA and protein expression links antibody genotype with phenotype. Yet, the ability to select and screen for high af...
Article
Recent developments in materials science and specific surface functionalization of materials are providing new tools for the rational design of precisely engineered drug delivery systems. Particular interest has been paid to the exploitation of functionalized materials in the administration of biopharmaceuticals by the oral route, traditionally cha...
Article
Full-text available
The electrospinning technique is being widely explored in the biomedical field due to its simplicity to produce meshes and its capacity to mimic the micro-nanostructure of the natural extracellular matrix. For skin tissue engineering applications, wound dressings made from electrospun nanofibers present several advantages compared to conventional d...
Conference Paper
Full-text available
Photocrosslinkable natural based hydrogel matrices have received great interest for tissue engineering strategies, especially because they provide versatile systems that can be potentially used in diverse applications. Nevertheless, to engineer complex tissues and/or tissue interfaces, it is necessary to develop systems that can accommodate various...