Pedro Mendoza de Gives

• PhD
• Head of Department at National Centre for Disciplinary Research in Veterinary Parasitology (CENID-Parasitología Veterinaria) INIFAP-Mexico

Cyrtocarpa procera is a tree from the Anacardiaceae family that has various biological properties such as antioxidant, antibacterial and anthelmintic. In this research, the in vitro ovicidal and larvicidal effect of a hydroalcoholic extract (HA-E) and two of its fractions (organic: EtAcO-F and aqueous: Aq-F) from dehydrated bark of C. procera on Ha...

Arthrobotrys oligospora is a nematode-trapping fungus belonging to the family Orbiliaceae and one of the main antagonists of nematodes in soil, including plant and animal parasitic nematodes and free-living nematodes. This species develops three-dimensional adhesive nets in which nematodes are trapped, destroyed and consumed by the fungus. It also...

Nematophagous fungi (NF) form part of the soil microbiota and are natural enemies of nematodes, helping to regulate nematode populations. A verticillate NF isolated from soil from Tepalcingo, Mexico, was morphologically and molecularly characterised. This fungus was cultured in two different liquid media—Czapek-Dox broth (CzDoxB) and sweet potato d...

Abstract: Background: Arthrobotrys species are nematophagous fungi that secrete extracellular nema-tocidal products (ECP). The individual and combined effects of ECP from Arthrobotrys oligospora (Ao) and A. musiformis (Am) growth in liquid media against Haemonchus contortus L3 (HcL3) were assessed. Methods: The isolation, morphological (MI) and mol...

his study describes the in vitro anthelmintic effect of a hydroalcoholic extract (HA-E) and its fractions from Cyrtocarpa procera fruits against Haemonchus contortus eggs and infective larvae. The HA-E was subjected to bipartition using ethyl acetate, which resulted in an aqueous fraction (Aq-F) and an organic fraction (EtOAc-F). The HA-E and both...

The individual and combined effects of extracellular products (ECP) from Arthrobotrys oligospora (Ao) and A. musiformis (Am) growth in liquid media against Haemonchus contortus L3 (HcL3) were assessed. The following methodological steps were established: isolation, morphological (MI) and molecular identification (Mol-I), assessment of nematocidal a...

Populations of Pasteuria penetrans isolated from root-knot nematodes ( Meloidogyne spp.) and cyst nematodes ( Heterodera spp.) were tested for their ability to adhere to a limited selection of sheathed and exsheathed animal parasitic nematodes, free living nematodes, including Caenorhabditis elegans wild type and several srf mutants, and plant para...

The aim of the study was to compare the relative gene expression of Haemonchus contortus P-glycoprotein genes ( Hco-pgp ) between fourth (L 4 ), infective (L 3 ), and transitory infective (xL 3 ) larval stages as laboratory models to study ivermectin (IVM) resistance. The H. contortus resistant to IVM (IVMr) and susceptible to IVM (IVMs) strains we...

This study evaluated the ovicidal and larvicidal effects of a hydroalcoholic extract (HAE) and two fractions-one aqueous (Aq-F) and the other organic (EtOAc-F)-from dehydrated Cyrtocarpa procera leaves on Haemonchus contortus. In addition, the primary compounds in the HAE and the fractions were identified. The egg hatching inhibition (% EHI) and L3...

This is the first record of the fungus Flavocillium subprimulinum in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek–Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal activity (NA) of three FLCF concentrations was assessed against Haemon...

The objective was to evaluate the nematocidal effect of four hydroalcoholic extracts (HAE) of Brosimum alicastrum (HAE-Ba), Guazuma ulmifolia (HAE-Gu), Erythrina americana (HAE-Ea) and Leucaena leucocephala (HAE-Ll) against Haemonchus contortus. The tests of egg hatching inhibition (EHI) and larval (infective larvae) mortality were used. The treatm...

Consumer awareness of animal welfare and environmental health has led to a plateau level of global consumption putting serious pressure on the livestock industry [...]

https://libreria.uady.mx/products/diagnostico-y-control-sustentable-de-nematodos-gastrointestinales-en-ovinos-y-caprinos-en-la-era-de-la-resistencia-antihelmintica Los nematodos gastrointestinales (NGI) son parásitos que afectan la salud y producción de los ovinos y caprinos en pastoreo. Su control se basa en el uso de medicamentos desparasitantes...

Acacia bilimekii is a plant with a high content of protein, fibre, and condensed tannins, making it an excellent feed for small ruminants with anthelmintic potential. This study aimed to evaluate the ovicidal activity of a hydroalcoholic extract (Ab-HA) and fractions from A. bilimekii aerial parts on Haemonchus contortus. The ovicidal activity of t...

Cooperia punctata is one of the most prevalent gastrointestinal nematodes affecting cattle under grazing conditions, and the increasing reports of anthelmintic resistance forces researchers to look for novel control measures. Previous reports have proposed the use of polyphenolic compound (PC) combinations (Coumarin:Quercetin (CuQ) and Caffeic-acid...

Artemisia cina is a plant used in traditional Chinese medicine as a remedy for parasitic diseases. This study describes the isolation and chemical characterization of anthelmintic compounds of A. cina against Haemonchus contortus infective larvae (L3) through lethal testing. Previously , three extracts-n-hexane (HexAc), ethyl acetate (EtOAc) and me...

The purpose of the present study was to assess the ovicidal and larvicidal activity of a hydroalcoholic extract (HAE) and their fractions (aqueous, Aq-F and organic, EtOAc-F) from Guazuma ulmifolia leaves using Haemonchus contortus as a biological model. The egg hatching inhibition (EHI) and larval mortality against infective larvae (L3) tests were...

The purpose of the present study was to assay the in vitro and in vivo anthelmintic activity (AA) of Caesalpinia coriaria (Cc) mature fruits against the nematode Haemonchus contortus (Hc). The Hc infective larvae were used to assess the in vitro AA through larval mortality assay. The exposure of larvae to the different treatments was performed in 9...

Pithecellobium dulce (Robx.) Benth is an arboreal legume used in traditional medicine for the treatment of several ailments, including a number of intestinal disorders, and as a natural deworm-ing. The objective of this study was to evaluate the ovicidal activity of a hydroalcoholic extract (HA-E) and its fractions (aqueous, Aq-F and organic, EtOAc...

Haemonchus contortus (Hc) is a parasite affecting small ruminants worldwide. Arthrobotrys musiformis (Am) is a nematode-trapping fungi that captures, destroys and feeds on nematodes. This study assessed the predatory activity (PA) and nematocidal activity (NA) of liquid culture filtrates (LCF) of Am against Hc infective larvae (L3), and additionall...

The nematocidal activity of an Oxalis tetraphylla hydroalcoholic extract against the nematode Haemonchus contortus (Hc) was assessed in vitro and the major compounds associated with nematocidal activity were identified. One hydroalcoholic extract was obtained from O. tetraphylla stems and leaves (Ot HE-SLE). The in vitro lethal concentrations (LC50...

Soil-borne parasitic nematodes cause severe deterioration in the health of crops and supply animals, leading to enormous economic losses in the agriculture and livestock industry worldwide. The traditional strategy to control these parasites has been based on chemically synthesised compounds with parasiticidal activity, e.g., pesticides and anthelm...

The ovicidal and larvicidal effect of a full aqueous extract (FE) and two phases: an aqueous (Aq-Ph) and an ethyl acetate (EtOAc-Ph) from Ruta chalepensis (Rc) stems and leaves against Haemonchus contortus (Hc) were assessed. The egg hatching inhibition (EHI) assay and larval mortality (LM) test were performed by triplicate in 96-well micro-titrati...

The upregulation of P-glycoprotein genes in Haemonchus contortus suspected of ivermectin resistance (IVM-Res) was studied in sheep farms. A faecal egg count reduction test (FECRT) was used to evaluate IVM-Res on four farms (from A to D). Faecal samples positive to gastrointestinal nematodes (GINs) were used to identify infective larval stages (L3)...

The objective of this study was to evaluate an n-hexane extract of Artemisia cina (Acn-h) as a natural anthelmintic treatment for periparturient goats naturally infected with the nematodes Haemonchus contortus and Teladorsagia circumcincta. A total of 200 periparturient Alpine and Nubian goats were used. Deworming criteria were based on the followi...

The objective of this study was to assay the ovicidal and larvicidal effects of two hydroalcoholic extracts (HA-E) and their aqueous fractions (Aq-F) and organic fractions (EtOAc-F) from Lippia graveolens (leaves) and Delonix regia (flowers) against the parasitic nematode Haemonchus contortus. The egg hatching inhibition test (% EHI) and mortality...

Mushrooms have health benefits, including anti-tumoral properties. We evaluated the cytotoxic and cell death induction effects of water-soluble extracts of Pleurotus ostreatus and Pleurotus eryngii mycelium in the cervical cancer cell lines HeLa (HVP18+) and SiHa (HVP16+) as well as the non-tumoral cell line HaCaT. Both Pleurotus extracts presented...

Ruminant parasitic nematodes (RPN) severely damage both the health and productivity of animals, followed by important economic losses in livestock production systems. The use of chemical anthelmintic drugs (AD) is a common practice of control; however, the development of anthelmintic resistance (AR) in the parasites has become a growing problem. Ad...

Aim This study was designed to investigate if culturing nematophagous fungi (NF) in the presence of a Haemonchus contortus larva crude extract (HcCE) enhances the nematocidal activity of nematophagous fungi liquid culture filtrates (NFCF). Materials and Methods Four NF Arthrobotrys oligospora, A. musiformis, Duddingtonia flagrans and Clonostachys...

The consumption of Caesalpinia coriaria dry ground fruit (Ccgf) and a Duddingtonia flagrans chlamydospore suspension (DfchS) either individually or in combination for the control of gastrointestinal nematodes (GIN) was assessed in sheep. Forty-five lambs infected with GIN were distributed into five groups (n = 9): Group 1: DfChS at 5 × 10⁵ chlamydo...

Previous research has shown an increased action on helminth biological control by fungal combinations. This study characterized the temperature and pH conditions necessary for better mycelial growth of \textit{Monacrosporium sinense} (SF53) and \textit{Pochonia chlamydosporia} (VC4). In addition, electron and optical microscopy showed the fungal st...

A batch of nematophagous fungi (NF), isolated from soil and animal faeces at Tapachula, Chiapas, Mexico, was identified through morphological methods and molecular tools. Additionally, the fungi were biologically characterised through their in vitro predatory capability against the free-living nematode Panagrellus redivivus. Molecular identificatio...

This review describes the advances acquired and proven in the use of helminthophagous fungi in the control of gastrointestinal helminth parasites in domestic animals. Old and well-known premises about parasitic epidemiology and the factors that can interfere with the best performance of biological control are mentioned. Some of the most promising f...

This study aimed to evaluate the effect of supplementing the maintenance diet of Boer goat kids with Acacia cochliacantha leaves. The endpoints evaluated were Haemonchus contortus fecal egg count (FEC) and water and dry matter intake. Two experimental treatments were evaluated on ten recently weaned goat kids (16.850 ± 1.630 kg of initial live weig...

The aim of the study was to evaluate the in vitro nematicide activity of the allspice (Pimienta dioica) and oregano (Origanum vulgare) essential oils (EOs) on eggs and larvae of gastrointestinal nematodes (GIN) from ruminant. The EOs were obtained by hydro-distillation of previously dehydrated plant material. In the in vitro evaluation, the egg hat...

This chapter provides a general overview of the main aspects related to the Fabaceae (Leguminosae) family, focusing on species of the genus Prosopis and covering their main aspects, such as nutritional characteristics, beneficial aspects in different social strata as well as their usage as food, traditional medicine and other uses. General informat...

Background. The edible mushroom Pleurotus ostreatus is a potential alternative for the control of gastrointestinal nematodes due to their nematoxins and secondary metabolites. Objective. To evaluate the in vitro anthelmintic activity of hydroalcoholic extracts of mycelium, basidiomata and spent substrate of the edible mushroom P. ostreatus against...

The objective of the study was to determine the parasitological and immune response in horses naturally re-infected with gastrointestinal nematodes (GIN) after anthelmintic treatment in the humid tropic of Mexico. The study was conducted in Tenosique, Tabasco, Mexico. A total of 30 horses were sampled monthly for nine months. Fecal samples were obt...

This study assessed the anthelmintic activity of the oral administration of a free-spore culture filtrate of the nematophagous fungus (NF) Arthrobotrys musiformis (M-10) on gastrointestinal parasitic nematodes (GIN) in naturally infected lambs. The fungus was grown on potato-dextrose agar plates (PDA) and transferred to a fermented rice medium (FRM...

Purpose(1) To assess the in vitro activity of Artemisia cina against Haemonchus contortus L3 (HcL3) and in transitional (L3–L4) larvae (HcTrL3–L4); (2) to quantify the relative expression of the Hc29 gene in HcTrL3–L4 exposed to the A. cina n-hexane extract; and (3) to assess the anthelmintic activity (AA) of the A. cina organic extracts in gerbils...

Four nematophagous fungi (NF) were identified by traditional and molecular techniques, and their in vitro predatory activity (PA) against Haemonchus contortus (Hc), and Panagrellus redivivus (Pr) was assessed. Additionally, the nematocidal activity (NA) of fungal organic extracts (OEs) obtained from a methanol/dichloromethane system was assessed. F...

This study evaluated in vitro the morphology of ten strains of edible mushroom: Pleurotus ostreatus (strains 1123 and 0152), P. eryngii (strains 1290 and 1291), P. cornucopiae (strains 1328 and 1330), Coprinus comatus (strain 1103), Panus sp. (strain 801), Lentinula edodes (strain 401) and L. boryanus (strain 402) on different growing media. In add...

This study was aimed at evaluating the in vitro effect of the edible mushroom (EM) Pleurotus eryngii against the eggs and larvae (L3) of Haemonchus contortus. The evaluation included acetone (AE) and hydroalcoholic (HA) extracts of the following strains: ECS-1138, ECS-1156, ECS-1255, ECS-1258, ECS-1261, ECS-1282, and ECS-1292. The HA extract of the...

Acacia farnesiana pods are rich in secondary metabolites and their biological activities have been recorded as antibacterial, antioxidant and anthelmintic. Previously, an in vitro bioguided study showed the important ovicidal and larvicidal effects of an organic fraction (EtOAc-F) from a hydroalcoholic extract of A. farnesiana pods against Haemonch...

Sheep haemonchosis is a disease that causes anemia, diarrhea, weight loss, low production, and even death of young animals. This represents a negative economic impact on the livestock sector. For decades, chemicals have been used to control this parasitic disease; however, cases of anthelmintic resistance have increased around the world. For this r...

The present paper reviewed publications on the nematocidal activity of edible mushrooms (EM) and their potential use as sustainable tools for the control of parasitic nematodes affecting agriculture and livestock industry. Nematodes are organisms living in the soil and animals’ guts where they may live as parasites severely affecting economically i...

AimThis study assessed the feeding activity of the predatory nematode Butlerius butleri on the infective larvae of the sheep parasitic nematode Haemonchus contortus (Hc-L3) and on two free-living nematodes Panagrellus redivivus (Pr) and Rhabditis sp. (R) in sterile sheep faecal cultures.Materials and MethodsA bioassay was carried out in sheep faeca...

Haemonchus contortus affect small ruminants all over the world. Anthelmintics cause resistance, contamination, and a risk of public health. Prosopis laevigata is a plant used as a home remedy against many diseases in Mexico. This study arose from a preliminary study where a P. laevigata hydroalcoholic extract (Pl-hae) showed anthelmintic activity (...

The objective of this study was to analyze the mRNA transcription levels of ten functional genes of P-glycoproteins (P-gp) in free life stages, eggs and infective larvae (L3) and in endoparasitic stages, fourth larval stage (L4) and adult males of two native isolates of Haemonchus contortus: resistant and susceptible to IVM. The IVM resistant isola...

The in vitro effect of silver nanoparticles of the Duddingtonia flagrans filtrate enriched with chitin was evaluated on infective larvae of cyathostomins (L3). After biosynthesis, an assay was carried out with two experimental groups in microtubes, for a period of 24 hours: G1 (AgNP's-D. flagrans (43.4 μg/mL) + 120 L3) and G2 (distilled water + 120...

Haemonchus contortus is a haematophagous gastrointestinal nematode (GIN) that causes severe anaemia and even death in small ruminants, negatively impacting the economic viability of farms. Traditionally, this parasite has been controlled with chemical compounds; however, inadequate use of these types of products has favoured the emergence of anthel...

Ethnopharmacology relevance Acacia farnesiana (L.) Willd is a shrub legume used as condiment, medicinal plant and bioactive herbage. This species is used in traditional medicine of several countries to relieve the symptoms of gastrointestinal diseases, diarrhoea, stomach pain and typhoid as well as astringent, antidysenteric and anthelmintic. Some...

In this chapter we present an overview of the use of molecular techniques applied to beneficial microorganisms as biotechnological tools for the control of pathogens and pests of agricultural plants. The content of the chapter addresses issues such as: agricultural pests, diversity of pests that affect economically important media: viruses, bacteri...

The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriaria tree, against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were...

The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriaria tree, against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were...

Gastrointestinal parasite infections represent a major welfare problem in small ruminants reared in extensive systems, which may be exacerbated by anthelmintic resistance. Therefore, we aimed to study the efficacy of albendazole and ivermectin in sheep. Eighty-six animals were selected from commercial farms in the temperate area of the State of Mex...

Polyphenolic compounds (PCs) have been proposed as one of the most bioactive group of secondary metabolites occurring in nature and have been associated to anthelmintic (AH)-like activity of plants against cattle nematodes. However, little is known regarding their synergetic / antagonistic interactions. This study assessed the in vitro AH-like acti...

Resumen El objetivo del presente estudio fue evaluar la toxicidad de Bencimidazol (BZ) y Lactona Macrocíclica (LM) en nematodos (NGI) de ovinos y confirmar la resistencia a BZ por la técnica de alelo específico-PCR (AS-PCR). El estudio se realizó en cinco municipios del Estado de Puebla, México con más de 700 ovinos. En total se evaluaron 10 y 7 gr...

This study assessed the in vitro anthelmintic (AH) activity of methanol and acetone:water leaf extracts from Annona squamosa , A. muricata and A. reticulata against Haemonchus contortus eggs. The egg hatch test was used to determine the effective concentrations required to inhibit 50% of eggs hatching (EC 50 ). The role of polyphenols on AH activit...

Antimicrobial resistance has been increasing in recent years and is most frequently found in pathogenic microorganisms resistant or multiresistant to drugs. The secondary metabolites of plants have been evaluated as alternatives for control and treatment of these microorganisms. The aim of this study was to isolate and identify the secondary metabo...

Nematophagous fungi from the feces of water buffalo and soil from southeastern Mexico were isolated, and their in vitro predatory activity against Haemonchus contortus infective larvae (L3) (HcL3) was assessed. The fungi were isolated by sprinkling soil or feces on water agar plates. Six series of 10 Petri dishes containing a 7-day-old culture of e...

Gastrointestinal nematodes (GIN) are responsible for enormous economic losses worldwide. The use of anthelmintic drugs reduces the parasitic burden in ruminants. However, the excessive use of these drugs triggers anthelmintic resistance in these parasites, which leads to a worrisome inefficacy of most of the commercially available antiparasitic dru...

The aim of this study was to evaluate the in vitro lethal effect of a methanolic extract (ME) from Caesalpinia coriaria fruits against Haemonchus contortus eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME was assessed using five concentrations as follows:...

This research assessed the dose/response to Duddingtonia flagrans chlamydospores (Df-C) administered to calves naturally infected with gastrointestinal nematodes (GINs), and its effect in reducing the infective larvae (L3) population in faeces in a farm in the Mexican tropics. Forty zebu calves, between 6 and 12 months of age, were randomly distrib...

The main objective was to determine the immune response of Blackbelly lambs infected with Haemonchus contortus, Trichostrongylus colubriformis, or both species. In the first stage, an experimental challenge was performed with 200 infective larvae (L3)/kg body weight (BW) of H. contortus (Hct, n = 8). Additionally, eight animals were infected with 5...

The influence of the physiological stage of a Blackbelly flock against natural infection by gastrointestinal parasitic nematodes (GIN) was investigated. Data on serum and saliva IgA levels and peripheral cellular immune response were recorded in 51 sheep for 6 months. The flock was divided based on their physiological stage as follows: a) pregnant...

An in vitro study was conducted to determine the anthelminthic activity of hydro-methanolic extracts of Larrea tridentata on sheathed and exsheathed larvae of Haemonchus contortus. Larvae of the parasite were incubated at 20–25 °C in hydro-methanolic extracts at concentrations of 12.5, 25, 50, 100, and 200 mg/mL for 24, 48, or 72 h. Ivermectin and...

The in vitro nematicidal effect of Chenopodium ambrosioides and Castela tortuosa n -hexane extracts (E-Cham and E-Cato, respectively) on Haemonchus contortus infective larvae (L3) and the anthelmintic effect of these extracts against the pre-adult stage of the parasite in gerbils were evaluated using both individual and combined extracts. The in vi...

This study was aimed to evaluate the in vitro lethal activity of the nematophagous fungi Clonostachys rosea against 5 nematodes species belonging to different taxa. Two groups of 35 Petri dishes (PD) each were divided into 5 series of 7 (PD). Group 1 (series 1, 2, 3, 4, and 5) contained only water agar; meanwhile group 2 plates (series 6, 7, 8, 9,...

The aims of this study were: 1) to assess the anthelmintic effect of Gliricidia sepium on the establishment of C. punctata third-stage larvae (L3) in calves, and 2) to isolate and to elucidate an anti-exsheathment phytochemical from the plant offered during the trial. Twelve ¾ Holstein × Zebu calves were divided in two experimental groups: control...

This research was performed to assess the Duddingtonia flagrans chlamydospore dose/response administered in gastrointestinal parasitic nematode (GIN) naturally infected calves and its effect in reducing the GIN infective larvae (L3) population in faeces in a cattle farm in the Mexican tropics. This study was carried out at the experimental research...

One metabolite against lamb gastrointestinal nematodes (GIN) was identified from extracts of Lysiloma acapulcensis leaves. Firstly, an aqueous extract of L. acapulcensis leaves and two fractions, organic and aqueous, were evaluated at different concentrations against Haemonchus contortus eggs and histotrophic larvae. In other experiment, 35 Pelibue...

A number of parasitic diseases seriously affect animal/crop production worldwide. Traditionally, the use of chemical anthelmintic drugs or pesticides is considered the commonest method of controlling such parasites. However, this has triggered an imminent risk to public health due to the continuous exposure of people to those chemical products. In...

Pleurotus djamor is an edible mushroom that has medicinal properties. This study aimed to assess the in vitro activity of P. djamor extracts and fractions against Haemonchus contortus eggs and exsheathed infective larvae (L3). Crude hydroalcoholic extracts were obtained by maceration and fractions were obtained through chromatography. Metabolite id...

The effect of hydroalcoholic extracts of fruits and leaves from Caesalpinia coriaria on the egg hatching inhibition of Haemonchus contortus and Haemonchus placei was evaluated. The tested concentrations were 25.0, 12.5, 6.2, 3.1, and 1.5 mg/mL; likewise, a 4% methanol solution and albendazole were used as negative and positive controls, respectivel...

Background: It is necessary to identify phenotypic traits related to natural resistance against gastrointestinal nematodes (GIN) in order to know the host immunity status in productive ewes. Objetive: To determine haematological and immunological parameters (IgA and IgG) during pregnancy and lactation in Blackbelly ewes naturally infected with GIN...

The in vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla hydroalcoholic extract (HE), against Haemonchus contortus in experimentally infected lambs, were assessed. We used a microtitration plate method, comprising the following two stages. Stage 1: 20 μl of water containing 200 sheathed H. contortus infective larvae (ShHcl) we...

The larval exsheathment inhibition assay (LEIA) of infective larvae (L3) is an in vitro method used to evaluate the anthelmintic (AH) activity of tannin-containing plant extracts against different species of gastrointestinal nematodes, including Haemonchus contortus. Some conditions remain to be defined in order to standardize the LEIA, i.e. the op...

Gliricidia sepium is a tropical legume with known anthelmintic-like properties. The aim of this study was to: (1) perform a bio-guided fractionation of an acetonic extract of G. sepium leaves using the egg hatch assay (EHA); (2) elucidate the anthelmintic (AH)-like phytochemical using nuclear magnetic resonance (NMR); and (3) assess the ultrastruct...

Argemone mexicana, Taraxacum officinale, Ruta chalepensis and Tagetes filifolia are plants with deworming potential. The purpose of this study was to evaluate methanolic extracts of aerial parts of these plants against Haemonchus contortus eggs and infective larvae (L3) and identify compounds responsible for the anthelmintic activity. In vitro prob...

The aim of this study was to evaluate the in vitro lethal effect of a hydroalcoholic extract (HAE) from Acacia cochliacantha leaf against three gastrointestinal nematodes species (Haemonchus contortus, H. placei and Cooperia punctata) of domestic ruminants. The HAE was assessed using five concentrations: 100, 125, 175, 150 and 200 mg/ml; 0.5% Iverm...

The expression profiles of cytokines and anti-oxidant genes were determined from an experimental infection with H. contortus in Pelibuey lambs. The infection was followed for 34 days (d) to determine the number of eggs per gram (epg) and the packed cell volume (PCV). Differential white cell counts and expression profile estimations of IL2, IL4, IL5...

The purpose of this study was to evaluate the predatory activity of the nematode Butlerius spp. and fungal isolates of Duddingtonia flagrans, Clonostachys rosea, Arthrobotrys musiformis and Trichoderma esau against H. contortus infective larvae (L3 ) in grass pots. Forty-eight plastic gardening pots containing 140 g of sterile soil were used. Panic...

This study determined the immunoglobulin (A and G) activity against gastrointestinal nematodes (GIN) and also the hematological parameters in four beef-calves breeds naturally infected in a tropical region of Mexico. Thirty-six infected calves were used to determine the fecal nematode egg counts (FEC), the IgA and IgG activity in serum and saliva,...

This study determined the efficacies of four classes of anthelmintics (AH) in a goat flock where anthelmintic resistance (AR) to ivermectin was suspected. We selected and randomly distributed 105 animals with >500 eggs per gram of feces into seven groups of 15 animals: an untreated control group and groups treated with ivermectin, eprinomectin, alb...

Two groups of six Haemonchus contortus infected Saint Croix lambs each received different diets for 11 weeks: control group, commercial food, molasses and lucerne hay; and treated group, nutritional pellets (NPs) containing Duddingtonia flagrans at 2 × 10 ⁶ chlamydospores/kg body weight (BW), sorghum and lucerne hay. Mean BW gain (BWG), body condit...

Background: Trichostrongylus colubriformis is one of the most prevalent gastrointestinal nematodes of sheep in warm climates. In this nematode species, anthelmintic resistance (AR) has been reported, which has motivated the search for alternative methods to control it. One practice is to increase the immune response level through the selection of a...

The usefulness of pellets industrially manufactured with spores of parasiticide fungi as a contribution to integrated nematode control was assessed in grazing horses throughout sixteen months. Two groups of 7 Pura Raza Galega autochthonous horses (G-T and G-P) were dewormed pour-on (1 mg Ivermectin/kg bw) at the beginning of the trial, and other gr...

Bioactive plants might represent an alternative for Cooperia punctata control in grazing cattle. The objectives of this study were (1) to assess the ovicidal activity of extracts from 4 plant species against C. punctata, (2) to determine the role of the polyphenols in the plants’ anthelmintic (AH) activity, and (3) to evaluate the best plant extrac...

Antihelmintic activity against Haemonchus contortus was searched as a new alternative for animal health improvement using three plants (Cydista aequinoctialis L., Heliotropium indicum L. and Momordica charantia L.) of traditional usage in the rural area of Tabasco State, Mexico. Aqueous extracts were obtained of dried material. Each extract was dil...