Pedro M. Aparicio-Tejo

Pedro M. Aparicio-Tejo
Universidad Pública de Navarra | UPNA · Natural Environment Sciences

Professor
Next August 31, I will be retired

About

145
Publications
18,989
Reads
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4,486
Citations
Citations since 2017
27 Research Items
1854 Citations
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20172018201920202021202220230100200300
20172018201920202021202220230100200300

Publications

Publications (145)
Article
Full-text available
Background Agriculture relies on the intensive use of synthetic nitrogen (N) fertilizers to maximize crop yields, which has led to the transformation of agricultural soils into high-nitrifying environments. Nevertheless, nitrification inhibitors (NIs) have been developed to suppress soil-nitrifier activity and decrease N losses. The NIs 3,4-dimethy...
Article
Full-text available
Agriculture has increased the release of reactive nitrogen to the environment due to crops' low nitrogen-use efficiency (NUE) after the application of nitrogen-fertilisers. Practices like the use of stabilized-fertilisers with nitrification inhibitors such as DMPP (3,4-dimethylpyrazole phosphate) have been adopted to reduce nitrogen losses. Otherwi...
Article
Atmospheric carbon dioxide (CO2) is increasing, and this affects plant photosynthesis and biomass production. Under elevated CO2 conditions (eCO2), plants need to cope with an unbalanced carbon-to-nitrogen ratio (C/N) due to a limited C sink strength and/or the reported constrains in leaf N. Here, we present a physiological and metabolic analysis o...
Article
Full-text available
Background The increasing demand for food production has led to a tenfold increase in nitrogen (N) fertilizer use since the Green Revolution. Nowadays, agricultural soils have been turned into high-nitrifying environments that increase N pollution. To decrease N losses, synthetic nitrification inhibitors (SNIs) such as 3,4-dimethylpyrazole phosphat...
Article
Nitrification inhibitors (NIs) are useful tools to reduce nitrogen (N) losses derived from fertilization in agriculture. However, it remains unclear whether a future climate scenario with elevated CO2 could affect NIs efficiency. Thus, the objective of this work was to study whether the increase of atmospheric CO2 concentration would affect the eff...
Article
Full-text available
Nitrate and ammonium are the main nitrogen sources in agricultural soils. In the last decade, ammonium (NH4+), a double-sided metabolite, has attracted considerable attention by researchers. Its ubiquitous presence in plant metabolism and its metabolic energy economy for being assimilated contrast with its toxicity when present in high amounts in t...
Article
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Low atmospheric relative humidity (RH) accompanied by elevated air temperature and decreased precipitation are environmental challenges that wheat production will face in future decades. These changes to the atmosphere are causing increases in air vapor pressure deficit (VPD) and low soil water availability during certain periods of the wheat-growi...
Chapter
This chapter presents a study of nitrous oxide (N2O) emissions from (a) forest soils (Pinus radiata and Fagus sylvatica) at different growth stages and from (b) grassland and agricultural soils in the edapho-climatic conditions of northern Spain. A mixed clover–ryegrass sward, wheat and oats crops were fertilized with ammonium sulphate nitrate, cat...
Article
The low-auxin-sensitivity tomato mutant, dgt, despite displaying reduced plant growth, has been linked to greater resistance to N deficiency. This led us to test the role of auxin resistance of dgt in NH4⁺ toxicity and N deficiency, compared to wild type tomato (cv. Micro-Tom, MT), grown in hydroponic media. A completely randomized design with thre...
Article
Nitrogen fertilization is a major force in global greenhouse gases emissions and causes environmental contamination through nitrate leaching. The use of nitrification inhibitors has been proven successful to mitigate these effects. However, there is an increasing concern about the undesired effects that their potential persistence in the soil or ac...
Article
Full-text available
Ammonium is an important nitrogen (N) source for living organisms, a key metabolite for pH control, and a potent cytotoxic compound. Ammonium is transported by the widespread AMT-Mep-Rh membrane proteins, and despite their significance in physiological processes, the nature of substrate translocation (NH 3 /NH 4⁺ ) by the distinct members of this f...
Article
Previous studies on Arabidopsis under long-term exposure to elevated CO2 have been conducted using starch synthesis and breakdown mutants cultured under short day conditions. These studies showed that starch synthesis can ameliorate the photosynthetic reduction caused by soluble sugar-mediated feedback regulation. In this work we characterized the...
Article
Nitrification inhibitors are used to maintain ammonium available in the soil for longer periods while reducing nitrate leaching and N2O emission. In this work we evaluated the potential toxicity effects of 3,4-dimethylpyrazole phosphate (DMPP) and 2-(N-3,4-dimethyl-1H-pyrazol-1-yl) succinic acid isomeric mixture (DMPSA) nitrification inhibitors. In...
Article
Full-text available
This paper presents a three-year pilot experience of a new municipal waste management system developed in Navarre, Spain that integrates composting and hens. The aim of this new system is to motivate the general public to participate more in waste prevention programs. The Composter-Henhouse (CH) is a compact facility comprised of a henhouse and thr...
Article
Full-text available
Background: The coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioni...
Article
Impairments in leaf nitrogen (N) assimilation in C3 plants have been identified as processes conditioning photosynthesis under elevated [CO2], especially when N is supplied as nitrate. Leaf N status is usually improved under ammonium nutrition and elevated [CO2]. However, ammonium fertilization is usually accompanied by the appearance of oxidative...
Article
Full-text available
In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during tw...
Data
Southern blot analysis of independent transgenic PpDof5 lines. 10 μg of genomic DNA from control plant (C) and each line (L1-L10, L12 and L13) were digested with BamHI and separated on an agarose gel. Blots were hybridized with 32-P labelled PpDof5 cDNA. (TIF)
Data
Expression analysis of PtrDof4 and PtrDof19. (A) Transcript levels were determined in stem (S) and leaves (L) of 10-weeks-old control hybrid poplar and L2 and L6 transgenics plants grown in a growth-chamber by qPCR using specific primers (S1 Table). (B) Transcript levels in side branches (B) and leaves (L) of poplar trees grown in the field. The ex...
Data
Histochemical analysis of lignin in basal sections of the stem. Cross-sections (10 μm thick) of control and transgenic lines were processed as described in Materials and Methods. The sections of stem were stained with phloroglucinol-HCl solution for detecting lignin. (TIF)
Data
Phylogenetic analysis. Phylogenetic tree of the deduced protein sequences of genes encoding Dof transcription factors from: Arabidopsis thaliana (36 sequences), Oryza sativa (31 sequences), Populus trichocarpa (41 sequences) and Pinus pinaster (10 sequences). As out of group were used two Dof sequences from Physcomitrella patens. The proteins are c...
Article
Two open-top chamber experiments were carried out at a continental and a coastal location in Spain to test the ozone sensitivity of spinach (Spinacia oleracea L.) and Swiss chard (Beta vulgaris L. var. cycla) under Mediterranean conditions. Three cultivars of spinach and two cultivars of Swiss chard currently grown in commercial fields were screene...
Article
Mediterranean soils, which are carbonate-rich and organic matter-poor, are prone to erosion and important carbon losses due to seasonal changes associated with dry summers and wet winters. The use of thermophilic digested sewage sludge (TSS) in these agricultural systems, as a soil amendment, has been acknowledged as an interesting way to supply or...
Article
Home composting is a powerful tool, which is spreading in different parts of the world, to reduce the generation of municipal waste. However, there is debate concerning the appropriateness, in terms of domestic hygiene and safety, of keeping a composter bin in the household deputed to kitchen waste of animal origin, such as meat or fish scraps and...
Article
Composting in bins is one of the most practical home composting methods. There is currently a need for greater information to improve the management of the composting process and to create home composting programs, which ensure sustainable production of high quality compost. This study investigates how two aspects of the bin feeding regime—the feed...
Article
Full-text available
Accessing different nitrogen (N) sources involves a profound adaptation of plant metabolism. In this study, a quantitative proteomic approach was used to further understand how the model plant Arabidopsis thaliana adjusts to different N sources when grown exclusively under nitrate or ammonium nutrition. Proteome data evidenced that glucosinolate me...
Article
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Background The use of reactive rock phosphate (RP) in acidic soils as a phosphate (P) source for pastures and crops presents attractive economic advantages with respect to soluble phosphate. However, some studies have demonstrated that the short-term (1-year) efficiency of RP, compared with that of water-soluble P, is relatively poor. This fact pen...
Article
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Although shoot N depletion in plants exposed to elevated [CO2 ] has already been reported on several occasions, some uncertainty remains about the mechanisms involved. The present study illustrates i) the importance of characterizing root-shoot interactions and ii) the physiological, biochemical, and gene expression mechanisms adopted by nitrate-fe...
Article
Poplar cuttings (Populus x euroamericana) were planted in pots and grown in a greenhouse for four months with drip irrigation. Two nitrogen (N) doses of 60 and 120 kg nitrate (N-NO3−) ha−1 (labelling with 15N stable isotopes) were applied at one (time I), two (time II) and three months after of the plantation (time III). The aim of this study was t...
Article
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The natural 15N/14N isotope composition (δ15N) of a tissue is a consequence of its N source and N physiological mechanisms in response to the environment. It could potentially be used as a tracer of N metabolism in plants under changing environmental conditions, where primary N metabolism may be complex, and losses and gains of N fluctuate over tim...
Article
A solution for environmentally wiser agriculture is the use of composted organic wastes as soil amendments. Just as this alleviates the problem of recycling organic residues, it provides necessary nutrient input for food production. The objective of this work was to study the effect that 13 years of applying three different composted organic wastes...
Article
An interesting alternative to landfills for disposing of organic residues is their addition to soil as composted organic residues. There is little information available about the long-term benefits following prolonged periods of application. After 12 years of annual incorporation of organic amendments to the soil of a vineyard, three soil character...
Conference Paper
Full-text available
Resumen: El trabajo realizado tiene por objetivo estudiar de qué forma influye la escala de compostaje en el proceso y el producto final, comparando compostaje a nivel industrial y doméstico. Para este fin se compostó el mismo tipo de residuos agroalimentarios en pilas volteadas (>1300 t) y paralelamente en contenedores de plástico de 320 L habitua...
Conference Paper
La utilización de compost de FORM como fertilizante es una práctica inocua y segura ampliamente implantada en la unión europea y que presenta gran cantidad de ventajas desde un punto de vista agronómico y medioambiental. La utilización de este tipo de compost como materia prima para la elaboración de sustratos de cultivo genera ciertas dudas por su...
Conference Paper
Full-text available
In order to asses the real ability of Fe-foliar applications to remedy Fe-deficiency in plants, we investigate usefulness of evaluating the main physiological plant root responses to Fe-deficiency at transcriptional and post-transcriptional levels.
Article
Background and aims Urea is the predominant form of N applied as fertilizer to crops, but it is also a significant N metabolite of plants themselves. As such, an understanding of urea metabolism in plants may contribute significantly to subsequent N fertilizer management. It currently appears that arginase is the only plant enzyme that can generate...
Data
Schematic showing effect of NBPT application on arginine catabolism and C-N metabolism in ammonium-fed plants. Metabolites, metabolic process and arrows depicted in red indicate fluxes hypothesized to be particularly strongly increased by reduction of endogenous urease (activity and expression). Blue arrows and process indicate fluxes hypothesized...
Data
Schematic showing effect of NBPT application on arginine catabolism and C-N metabolism in nitrate-fed plants. Metabolites, metabolic process and arrows depicted in red indicate fluxes hypothesized to be particularly strongly increased by reduction of endogenous urease (activity and expression). Blue arrows and process indicate fluxes hypothesized t...
Article
The main goal of this study was to test the effect of [CO2] on C and N management in different plant organs (shoots, roots and nodules) and its implication in the responsiveness of exclusively N2-fixing and NO3−-fed plants. For this purpose, exclusively N2-fixing and NO3−-fed (10 mM) pea (Pisum sativum L.) plants were exposed to elevated [CO2] (100...
Article
Full-text available
Although the predicted enhanced photosynthetic rates of plants exposed to elevated [CO2] are expected to increase carbohydrate and plant growth, recent findings have shown a complex regulation of these processes. The aim of this study was to determine the effect of elevated [CO2] on pathways leading to the main forms of leaf C storage (starch) and...
Conference Paper
Full-text available
The application of urban residues to soil, such as sewage sludge that have gone through a thermophilic anaerobic digestion, have the potential to act as mineral fertilizer replacements. The scope of this study was to determine some Nitrogen losses to the atmosphere, specifically nitrous oxide (N 2 O) emissions after 20 years of continued applicatio...
Article
Full-text available
Agencies for organic farming certification require the development of control techniques to control fertilisation management. Natural 15N abundance, expressed as δ15N, is considered as an integrated descriptor of the N historical cycle of an agrosystem, thus isotopic techniques has been proposed to verify the organic or inorganic nature of N fertil...
Article
An understanding of the mechanisms underlying ammonium toxicity in plants requires prior knowledge of the metabolic uses for nitrogen (N) and carbon (C). We have recently shown that pea plants grown at high ammonium concentrations suffer an energy deficiency associated with a disruption of ionic homeostasis. Furthermore, these plants are unable to...
Article
Full-text available
Sugar snap pea (Pisum sativum L. var. macrocarpon Ser.) is an edible-podded sweet pea that is being considered as a new totally mechanized crop to supply raw material to the agri-food industry of the Ebro Valley (Northern Spain). It is of great interest from an agronomic and commercial standpoint but neither its agronomic behaviour nor its adaptati...
Article
Three main families of SODs in plants may be distinguished according to the metal in the active center: CuZnSODs, MnSOD, and FeSOD. CuZnSODs have two sub-families localized either in plant cell cytosol or in plastids, the MnSOD family is essentially restricted to mitochondria, and the FeSOD enzyme family has been typically localized into the plasti...
Conference Paper
Full-text available
The aim of this work was to compare the effect of different organic and mineral fertilizers in terms of yield, bioavailability of nutrients, and heavy metals accumulation. Two trials were established in a greenhouse with lettuce and spinach cultivated in pots, to assess the suitability as fertilizers of 7 compost (4 homemade composts obtained from...
Article
A careful control of the N nutritional status of grapevines can have a determining effect on wine characteristics; therefore a suitable management of N fertilization might allow some wine parameters to be modified, thereby improving product quality. The aim of this study was to determine the effect of foliar application of urea at different doses a...
Article
Full-text available
The effect of the nitrogen source (ammonium and nitrate) and its interaction with magnesium on various physiological processes was studied in sunflower plants (Helianthus annuusL.). Plants were grown in hydroponic culture with nitrate (5 mM) or ammonium (5 mM) and four concentrations of magnesium (0.1, 0.8, 5 and 10 mM). After 2 weeks, growth, gas...
Article
The development of new nitrogen fertilizers is necessary to optimize crop production whilst improving the environmental aspects arising from the use of nitrogenous fertilization as a cultural practice. The use of urease inhibitors aims to improve the efficiency of urea as a nitrogen fertilizer by preventing its loss from the soil as ammonia. Howeve...
Article
Full-text available
A new type of superphosphate (organic complexed superphosphate (CSP)) has been developed by the introduction of organic chelating agents, preferably a humic acid (HA), into the chemical reaction of single superphosphate (SSP) production. This modification yielded a product containing monocalcium phosphate complexed by the chelating organic agent th...
Conference Paper
Home composting is an eco‐friendly strategy in order to prevent organic municipal waste generation. More information is necessary to improve the process and to ensure the production of high quality compost susceptible to be employed without restrictions. One of the factors to be clarified is the effect of the feeding frequency of the compost bins w...
Article
The use of urea as an N fertilizer has increased to such an extent that it is now the most widely used fertilizer in the world. However, N losses as a result of ammonia volatilization lead to a decrease in its efficiency, therefore different methods have been developed over the years to reduce these losses. One of the most recent involves the use o...
Article
Full-text available
Two phylogenetically unrelated superoxide dismutase (SOD) families, i.e., CuZnSOD (copper and zinc SOD) and FeMn-CamSOD (iron, manganese, or cambialistic SOD), eliminate superoxide radicals in different locations within the plant cell. CuZnSOD are located within the cytosol and plastids, while the second family of SOD, which are considered to be of...
Data
Full-text available
Calculations appendix. The calculations used to achieve these results have been added to the manuscript to clarify the discussion and conclusions of this work. A) Calculations for obtaining the 15N content as μmol 15N·100 g-1 DW from the δ15N (‰) and total N content (% N). B) The 15N contents from the external NH4+ and NH3 were calculated using the...
Data
Full-text available
Natural isotopic signature data. Tables with plant biomass ratios of plants fed with NH4+/NO3- as the sole N source and δ15N values in shoots and roots of plants fed with NH4+ or NO3- as the sole N source.
Data
Control measures of external pH in all short-term experiments. Initial and final pH values of the external solutions at pH 6 (panels A, C and E) and 9 (panels B, D and F).
Article
Full-text available
In plants, nitrate (NO3-) nutrition gives rise to a natural N isotopic signature (δ15N), which correlates with the δ15N of the N source. However, little is known about the relationship between the δ15N of the N source and the 14N/15N fractionation in plants under ammonium (NH4+) nutrition. When NH4+ is the major N source, the two forms, NH4+ and NH...
Article
Full-text available
Nitrate leaching as a result of excessive application of N-fertilizers and water use is a major problem of vulnerable regions. The farming of maize requires high N fertilization and water inputs in Spain. Isotopic techniques may provide information on the processes involved in the N and C cycles in farmed areas. The aim of this work was studying th...
Article
The widespread use of NO(3)(-) fertilization has had a major ecological impact. NH(4)(+) nutrition may help to reduce this impact, although high NH(4)(+) concentrations are toxic for most plants. The underlying tolerance mechanisms are not yet fully understood, although they are thought to include the limitation of C, the disruption of ion homeosta...
Article
The form of nitrogen nutrition affects many biochemical and physiological processes in plants, leading to markedly different growth responses. Many plant species have been characterized as ammonium tolerant or sensitive. The objective of this work was to assess the range of physiological adaptative responses involved in tolerance of ammonium nutrit...