Paweł Czerniewicz

Paweł Czerniewicz
Siedlce University of Natural Sciences and Humanities · Chair of Biochemistry and Molecular Biology

PhD

About

68
Publications
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525
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Publications

Publications (68)
Article
Full-text available
This study investigated the toxicity of essential oils (EOs) from Santolina chamaecyparissus (L.) and Tagetes patula (L.) towards the green peach aphid Myzus persicae (Sulzer) and the bird cherry-oat aphid Rhopalosiphum padi (L.). The effects of the EOs on aphid population parameters and levels of biochemical markers of oxidative stress within inse...
Article
Polyamines (PAs) are involved in plant response to abiotic and biotic stresses, however, their role in biochemical insect-plant interactions is not clear. Therefore, we compared the involvement of polyamines and key enzymes of their biosynthesis in gall formation process. The present study had used galls on oak leaves caused by asexual generation (...
Article
The major aim of the present study was to investigate the influence of juglone (JU; 5-hydroxy-1,4-naphthoquinone) treatments on the expression level of Cat1, Cat2 and Cat3 genes, encoding the respective catalase isozymes in maize (Zea mays L.) and wheat (Triticum aestivum L.) seeds. In parallel, germination efficiency, catalase (CAT) activity and h...
Article
Full-text available
Analysis of Lamiaceae essential oils (EOs) by GC-FID-MS revealed the presence as the major constituents of linalool (16.8%), linalyl acetate (15.7%) in Lavandula angustifolia, menthol (29.0%), menthone (22.7%), menthyl acetate (19.2%) in Mentha x piperita, terpinen-4-ol (27.1%), (E)-sabinene hydrate (12.1%), γ-terpinene (10.0%) in Origanum majorana...
Article
The essential oils derived from Asteraceae plants were evaluated for their insecticidal activity towards Myzus persicae (Sulzer) and for their effects on the activity of selected enzymes within aphid tissues. The chemical composition of the essential oils was determined by GC-MS analysis. In laboratory bioassays, the studied oils not only displayed...
Article
Full-text available
The study aimed at comparing the changes in the content of free amino acids, phenolic compounds and the activity of PAL and TAL caused by two piercing-sucking arthropods: the grape mealybug (Pseudococcus maritimus Ehrh.) and the two-spotted spider mite (Tetranychus urticae Koch) in the leaves of orchid and strawberry, respectively. The obtained res...
Conference Paper
Full-text available
This report describe the changes in Ihe content o f plant biogenic amines (putrescine, cadaverine, spermidyne, tryptamine, spermine and histaminę) and key enzymes o f their biosynthesis: lysine decarboxylase (LDC), tyrosine decarboxylase (TyDC) and ornithine decarboxylase (ODC) in galls and other parts o f Siberian elm (Ulmus pumila L.) leaves duri...
Article
One of the earliest responses of plants to insects’ attack is generation of reactive oxygen species. However, the elevated level of ROS can elicit oxidative burst within plant tissues, and plants employ antioxidant systems against these radicals. Due to their chemical structures, polyphenols are able to diminish the level of ROS. Thus, we investiga...
Conference Paper
Full-text available
The study was aimed at evaluating influence of the bird cherry-oat aphid (Rhopalosiphum padi L.) infestation on relative expression of two genes (galdh and gldh, encoding L-galactose dehydrogenase and L-galactono-1,4-lactone dehydrogenase, respectively) in maize (Zea mays L.) plants. The analysed genes are involved in biogenetic pathway of reduced...
Article
Tetraneura ulmi (L.), a member of Eriosomatinae subfamily, is one of the gall-forming aphids occurring on elms. Sap sucking behaviour of founding mothers results in the formation of new plant organs. This study documents the changes in the content of plant biogenic amines (putrescine, cadaverine, spermidyne, tryptamine, spermine and histamine) and...
Article
Full-text available
The naturally-occurring phenolic acid mixtures from selected herbal plants were tested for their deterrent and aphidicidal activity towards two aphid species, the peach-potato aphid, Myzus persicae (Sulz.) and the bird cherry-oat aphid, Rhopalosiphum padi (L.). The phenolic acids were extracted from Hypericum perforatum (L.), Juglans regia (L.), Me...
Conference Paper
Full-text available
Gall formation is a unique group of interactions between the insect and the host plant. This paper presented the qualitative and quantitative structure of gall forming aphids (Hemiptera, Eriosomatinae) on elm and poplar trees and their influence on plants’ decorative value. The research was carried out in 2012, on Ulmus glabra, U. pumila, U. laevis...
Conference Paper
Full-text available
Amino acids and phenolic compounds play different roles in plant metabolism and biochemical defence against herbivorous arthropods. Amino acids are primary metabolites limiting the nutritive value of plant tissues for phytophagous insects, therefore secondary phenolic metabolites are generally regarded as defensive molecules. The study was aimed a...
Article
The study aimed to quantify the influence of common plant polyamines and tyramine on probing behaviour in the bird cherry-oat aphid ( Rhopalosiphum padi L.). Electrical penetration graphs (DC) were used to monitor the probing and feeding behaviour of R. padi exposed to the amines agmatine, cadaverine, putrescine, spermidine, spermine and tyramine....
Article
Full-text available
The purpose of the study was to assess the prevalence and coinfection rates of Borrelia burgdorferi sensu lato genotypes in Ixodes ricinus (L.) ticks sampled from diverse localities in central and eastern regions of Poland. In years 2009-2011, questing nymphs and adults of I. ricinus were collected using a flagging method at 18 localities represent...
Conference Paper
Full-text available
Lysine decarboxylase (LDC) plays a double role in plant metabolism. The enzyme participates in biodegradation of an important essential amino acid, lysine. On the other hand, LDC takes part in biosynthesis of cadaverine as a biomolecule involved in plant defense against pathogens. However, there are a few data in literature on LDC participation in...
Conference Paper
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Dzięki niskiej zawartości wtórnych metabolitów ograniczających żerowanie, wzrost i rozwój oraz rozmnażanie roślinożerców, sok floemowy jest głównym źródłem pokarmu dla mszyc i innych owadów kłująco-ssących. Zawiera on jednak niewielkie ilości substancji odżywczych, a zwłaszcza aminokwasów egzogennych. Stąd przemiany metaboliczne zmierzające do doda...
Conference Paper
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Jedną z alternatywnych metod ograniczania populacji szkodników jest stosowanie ekstraktów zawierających aktywne biologicznie wtórne metabolity roślinne. Należą do nich związki fenolowe, które ze względu na toksyczne właściwości oddziaływują niekorzystnie na wzrost i rozwój owadów. Jednak roślinożerne owady posiadają również mechanizmy obronne, umoż...
Conference Paper
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Celem wykonanych badań było porównanie profili ekspresyjnych dwóch wybranych genów szlaku biogenezy glutationu, kodujących odpowiednio syntetazę γ-glutamylocysteiny (GSH1) i syntetazę glutationu (GS1) w siewkach kukurydzy (Zea mays L.) zaatakowanych przez mszycę czeremchowo-zbożową (Rhopalosiphum padi L.). Dodatkowo, określono zawartość glutationu...
Conference Paper
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Plant L-phenylalanine ammonialyase (PAL) and L-tyrosine ammonialyase (TAL) catalyse deamination of L-phenylalanine and L-tyrosine to trans-cinnamic acid and p-coumaric acid, respectively. The both phenylpropenoic acids are next include into shikimic pathway that is source of some defence secondary metabolites participating in biochemical plant defe...
Article
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The objective of performed study was aimed at evaluating the antifungal potential of four extract fractions (methanolic, ethyl acetate, alkaloid, and hydrolysed methanolic) derived from Juglans regia (L.) leaves against pathogenic Candida albicans strains. Furthermore, total phenolics and alkaloid content as well as the antioxidative potential of e...
Article
Full-text available
In this study, the toxicity and mechanism of action of concanavalin A (ConA) in the grain aphid (Sitobion avenae) were studied. Feeding assays with S. avenae on an artificial diet containing different concentrations of ConA demonstrated an inhibitory effect on fecundity as well as high mortality caused by this lectin. ConA also increased the pre-re...
Article
Full-text available
The toxicity effect of Concanavalin A (Canavalia ensiformis lectin, ConA) to bird cherry-oat aphid, Rhopalosiphum padi L. (Hemiptera: Aphididae), was investigated in the laboratory by using artificial diets containing ConA concentrations. Bird cherry-oat aphid performance was affected by the presence of Con A in artificial diets. The lectin added i...
Article
Full-text available
The purpose of this report was to evaluate the expression patterns of selected glutathione transferase genes (gst1, gst18, gst23 and gst24) in the tissues of two maize (Zea mays L.) varieties (relatively resistant Ambrozja and susceptible Tasty Sweet) that were colonized with oligophagous bird cherry-oat aphid (Rhopalosiphum padi L.) or monophagous...
Article
Full-text available
The mechanism of the toxicity of plant lectins is not clearly understood. The insecticidal activity of plant lectins results from effects on insect metabolism by interfering with gut enzymatic function. Thus, a greater understanding of the mechanisms of plant lectin toxicity in insects is required. This study reports the effects of dietary ingestio...
Article
Full-text available
We measured the total chlorophyll (Chl a+b) content in seedling leaves of fifteen maize cultivars infested by two studied aphid species (oligophagous Rhopalosiphum padi L., monophagous Sitobion avenae F.) 7 and 14 days after the beginning of infestation, using a SPAD-502 chlorophyll meter. Chlorophyll loss was more severe in R. padi-infested than i...
Article
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In the current work, the toxicity and mechanism of phytohaemagglutinin (PHA), lectin isolated from the kidney bean (Phaseolus vulgaris) to the grain aphid (Sitobion avenae F.) were studied. When S. avenae was fed an artificial diet containing the lectin PHA, toxicity assays indicated that fecundity decreased, the pre-reproductive period and generat...
Article
Full-text available
Despite senescence-induced chlorophyll depletion in plants has been widely studied, the enzymatic background of this physiologically regulated process still remains highly unclear. The purpose of this study was to determine selected biochemical properties of partially purified fractions of chlorophyllase (Chlase, chlorophyll chlorophyllido-hydrolas...
Article
Full-text available
The toxicity and deterrent effects of phytoheamagglutinin (Phaseolus vulgaris agglutinin, PHA) to the grain aphid (Sitobion avenae F.) was investigated in the laboratory by use of liquid diets or gels containing various PHA concentrations. The addition of PHA to the liquid diet increased the aphid pre-reproductive period, mortality, and generation...
Article
Full-text available
The presented study aimed at establishing the prevalence and co-infection rates of Bartonella henselae and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from the central and eastern parts of Poland. The common tick individuals were gathered in the years 2008-2009. Questing ticks were sampled by dragging a white woollen flag over...
Article
Full-text available
The insecticidal activity of plant lectins against a wide range of insect species have been intensively studied. Understanding the mechanism of the toxicity of lectins is one of the studied aspects. In the present research, the first step was determine the effect of phytohemagglutinin (PHA) on the development, fecundity and mortality of grain aphid...
Article
Full-text available
Amino acid level is well known indicator of plant resistance to aphids. Our earlier studies showed that grain aphid (Sitobion avenae F.) infestation caused changes in the activity of the enzymes connected with amino acid biosynthesis and the transformation to defensive secondary metabolites within triticale tissues. However, there are not data on t...
Article
The aim of the study was to elucidate the distribution of Anaplasma phagocytophilum and Babesia microti co-infection in Ixodes ricinus populations within the central-eastern region of Poland. The prevalence of analysed tick-borne human pathogens in single and polymicrobial infections in I. ricinus ticks were analysed using the conventional and nest...
Article
Full-text available
The aim of the study was to elucidate the distribution of Anaplasma phagocytophilum and Babesia microti co-infection in Ixodes ricinus populations within the central-eastern region of Poland. The prevalence of analysed tick-borne human pathogens in single and polymicrobial infections in I. ricinus ticks were analysed using the conventional and nest...
Article
Full-text available
The objectives of this study were to elucidate the impact of bird cherry-oat aphid (Rhopalosiphum padi L.) feeding on functioning of the proteolytic machinery in bird cherry leaves. Biochemical analyses proved that R. padi feeding in tissues of primary host stimulated activity of the two major fractions of proteinases (extracted at the optimal pH v...
Article
The effects of phytohemagglutinin (PHA) on development, fecundity, and mortality of the grain aphid, Sitobion avenae (Fabricius), were determined. Also determined were the effects of PHA on the activity of gut enzymes involved in sugar and phosphorus metabolism (α- and β-glucosidase and alkaline and acid phosphatase) and on the feeding behavior of...
Article
Full-text available
Differences in the quantity and quality of phenolic compounds between the primary and secondary hosts of bird cherry-oat aphid (Rhopalosiphum padi L.) during its spring migration were studied. The aphid population developed on the primary host, the highest concentration of total phenolics was found in the mature leaves of bird cherry (Prunus padus...
Article
Full-text available
The chromatographic separations showed the presence of eleven free phenolic acids (trans-cinnamic, o-coumaric, p-coumaric, chlorogenic, ferulic, gallic, caffeic, p-hydroxybenzoic, syringic, tannic and vanillic) in the walnut tissues. The p-hydroxybenzoic acid was a predominant compound in the green husks (618.8 µg·g-1), though, high content of it w...
Conference Paper
Full-text available
Chrzanowski G., Leszczyński B., Czerniewicz P., Sytykiewicz H., Matok H., Krzyżanowski R., 2010. Phenolic acids of walnut Juglans regia L. 7th International Symposium on Chromatography of Natural Products (ISCNP), 14-17 June, Lublin (Poland), pp. 85.
Article
Full-text available
Effect of position in the canopy of bird cherry on the development of colonies of Rhopalosiphum padi L. The influence of light intensity, temperature and humidity on the growth and development of colonies of the bird cherry-oat aphid was studied. The south and southwestern sectors of the canopy of a clump of bird cherry trees, which were less shade...
Article
Full-text available
Journal of Polish Agricultural Universities is the very first Polish scientific journal published exclusively on the Internet, founded on January 1, 1998 by the following agricultural universities and higher schools of agriculture: ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS AND DISCUSSION CONCLUSIONS REFERENCES ABSTRACT The aim of study wa...
Article
Full-text available
INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS REFERENCES ABSTRACT Polyphenoloxidase (PPO) plays an important role in plant resistance to insects, and in the detoxication of phenolic compounds taken in the nutrient components. Activity of PPO was examined in the ears of spring wheat and triticale. Extraction of enzymatic protein...

Projects

Projects (3)
Project
Major purpose of the research is to elucidate transcriptional reprogramming of genes involved in generation of oxidative stress in tissues of cereal plants.
Project
Molecular detection a wide array of microorganisms transmitted by Ixodes ricinus populations, with special emphasis on the co-infection status.