Pawel Boguszewski

Pawel Boguszewski
Nencki Institute of Experimental Biology | NENCKI · Neurobiology Center

PhD

About

64
Publications
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837
Citations

Publications

Publications (64)
Article
Animals display a rich repertoire of defensive responses adequate to the threat proximity. In social species, these reactions can be additionally influenced by the behavior of fearful conspecifics. However, the majority of neuroscientific studies on socially triggered defensive responses focuses on one type of behavior, freezing. To study a broader...
Article
Full-text available
Respiratory disturbances present in Parkinson's disease (PD) are not well understood. Thus, studies in animal models aimed to link brain dopamine (DA) deficits with respiratory impairment are needed. Adult Wistar rats were lesioned with injection of 6-hydroxydopamine (6-OHDA) into the third cerebral ventricle. Two weeks after hypoxic test was perfo...
Article
Full-text available
Our rudimentary knowledge about rat intraspecific vocal system of information exchange is limited by experimental models of communication. Rats emit 50-kHz ultrasonic vocalizations in appetitive states and 22-kHz ones in aversive states. Both affective states influence heart rate. We propose a behavioral model employing exposure to pre-recorded pla...
Article
Full-text available
Our rudimentary knowledge about rat intraspecific vocal system of information exchange is limited by experimental models of communication. Rats emit 50-kHz ultrasonic vocalizations in appetitive states and 22-kHz ones in aversive states. Both affective states influence heart rate. We propose a behavioral model employing exposure to pre-recorded pla...
Article
Full-text available
Intravitreal delivery of brain-derived neurotrophic factor (BDNF) by injection of recombinant protein or by gene therapy can alleviate retinal ganglion cell (RGC) loss after optic nerve injury (ONI) or laser-induced ocular hypertension (OHT). In models of glaucoma, BDNF therapy can delay or halt RGCs loss, but this protection is time-limited. The d...
Article
Full-text available
Maternal immune activation (MIA), induced by infection during pregnancy, is an important risk factor for neuro-developmental disorders, such as autism. Abnormal maternal cytokine signaling may affect fetal brain development and contribute to neurobiological and behavioral changes in the offspring. Here, we examined the effect of lipopolysaccharide-...
Preprint
Full-text available
To survive, an animal must adequately respond to challenges presented by the environment. Social animals can learn about danger from fear of conspecifics which allows them to avoid predation and other threats without costly, first-hand experience. However, it remains unclear as to whether animals can transmit specific information that helps an indi...
Article
Full-text available
The maintenance of proper cytosolic Ca2+ level is crucial for neuronal survival, and dysregulation of Ca2+ homeostasis is found in a variety of neurological disorders, including Alzheimer’s disease. According to the “Ca2+ hypothesis of aging”, Ca2+ disturbances precede the onset of AD symptoms and lead to neurodegeneration. STIM and ORAI proteins a...
Article
Full-text available
Orai proteins form highly selective Ca²⁺ release-activated channels (CRACs). They play a critical role in store-operated Ca²⁺ entry (SOCE; i.e., the influx of external Ca²⁺ that is induced by the depletion of endoplasmic reticulum Ca²⁺ stores). Of the three Orai homologs that are present in mammals (Orai1–3), the physiological function of Orai1 is...
Article
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The angiomotin (Amot)–Yes-associated protein 1 (Yap1) complex plays a major role in regulating the inhibition of cell contact, cellular polarity, and cell growth in many cell types. However, the function of Amot and the Hippo pathway transcription coactivator Yap1 in the central nervous system remains unclear. We found that Amot is a critical media...
Preprint
Full-text available
The Amot-Yap1 complex plays a major role in the regulation of cell contact inhibition, cellular polarity and growth. However, the function of Angiomotin (Amot) and Hippo pathway transcription co-activator Yap1 in the central nervous system remains unclear. In this study, we demonstrate that Amot is a critical mediator of dendritic morphogenesis in...
Chapter
The effects of exogenous opioids on 50-kHz ultrasonic vocalizations (USVs) depend on their affinity to specific opioid receptors and a schedule of administration. Acute administration of morphine inhibits the emission of 50-kHz USVs, but repeated morphine administration reduces this suppressive effect and might even increase the occurrence of this...
Article
Full-text available
Research shows that interpersonal rejection increases aggression and decreases helping toward the rejecter. Based on the assumptions of the evolutionary approach, it was hypothesized that aggression would be higher and helping would be lower after rejection by a same-sex rather than an opposite-sex other. Moreover, it was predicted that the effect...
Article
Our previous studies have shown that ACPT-I [(1S, 3R,4S)-1-aminocyclopentane-1,2,4-tricarboxylic acid], a blood-brain barrier permeable agonist of group III metabotropic glutamate (mGlu) receptors, was neuroprotective against middle cerebral artery occlusion/reperfusion (MCAO/R) in normotensive rats. Preclinical studies are typically performed on h...
Article
It was postulated that neuropeptide Y (NPY)-ergic system could be involved in the ischemic pathophysiology, however, the role of particular subtypes of NPY receptors (YRs) in neuroprotection against ischemia is still not well known. Therefore, we investigated the effect of NPY and YR ligands using in vitro and in vivo experimental ischemic stroke m...
Article
Full-text available
Traffic noise was tested as a stress factor in an open-field study of urban and non-urban populations of two species belonging to the genus Apodemus: the striped field mouse and the yellow-necked mouse. The striped field mouse has inhabited green areas in cities for years, whereas the yellow-necked mouse was first observed in such areas relatively...
Article
Full-text available
STIM1 is an endoplasmic reticulum calcium sensor that is involved in several processes in neurons, including store-operated calcium entry. STIM1 also inhibits voltage-gated calcium channels, such as Cav1.2 and Cav3.1, and is thus considered a multifunctional protein. The aim of this work was to investigate the ways in which transgenic neuronal over...
Data
Eco-HAB measured social approach and in-cohort sociability of valproate-treated and control C57BL/6 and BALB/c mice.The names of the Excel sheets refer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see 'Materials and methods').DOI: http://dx.doi.org/10.7...
Data
Eco-HAB measured social approach and in-cohort sociability of valproate-treated and control C57BL/6 and BALB/c mice.The names of the Excel sheets refer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.755...
Data
Eco-HAB measured social approach and in-cohort sociability of valproate-treated and control C57BL/6 and BALB/c mice.The names of the Excel sheets refer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.755...
Data
Eco-HAB measured social approach and in-cohort sociability of Fmr1 knockouts and wild-type controls.The names of the Excel sheets refer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see 'Materials and methods').DOI: http://dx.doi.org/10.7554/eLife.19532....
Data
Eco-HAB measured social approach and in-cohort sociability of Fmr1 knockouts and wild-type controls.The names of the Excel sheetsrefer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see 'Materials and methods').DOI: http://dx.doi.org/10.7554/eLife.19532.0...
Data
Eco-HAB measured social approach score for valproate-treated and control C57BL/6 and BALB/c mice and Fmr1 knockouts and wild-type controls.These data are identical to Figure 3A—source data 1, Figure 3E—source data 3, Figure 4A—source data 1 with respect to Figures 3A,E and 4A and are available as a separate file for the readers’ convenience.DOI: ht...
Data
Eco-HAB measured social approach and in-cohort sociability of valproate-treated and control C57BL/6 and BALB/c mice.The names of the Excel sheets refer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.755...
Data
Eco-HAB measured social approach score for valproate-treated and control C57BL/6 and BALB/c mice and Fmr1 knockouts and wild-type controls.These data are identical to Figure 3A—source data 1, Figure 3E—source data 3, Figure 4A—source data 1 with respect to Figures 3A,E and 4A and are available as a separate file for the readers’ convenience.DOI: ht...
Data
We include source data for Figures 6, 7 and 8 concerning reproducibility results of both Eco-HAB measures.The names of the Excel sheets refer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see 'Materials and methods').DOI: http://dx.doi.org/10.7554/eLife....
Data
We include source data for Figures 6, 7 and 8 concerning reproducibility results of both Eco-HAB measures.The names of the Excel sheetsrefer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.7554/eLife.195...
Data
Eco-HAB measured social approach score for valproate-treated and control C57BL/6 and BALB/c mice and Fmr1 knockouts and wild-type controls.These data are identical to Figure 3A—source data 1, Figure 3E—source data 3, Figure 4A—source data 1 with respect to Figures 3A,E and 4A and are available as a separate file for the readers’ convenience.DOI: ht...
Data
We include source data for Figures 6, 7 and 8 concerning reproducibility results of both Eco-HAB measures.The names of the Excel sheetsrefer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.7554/eLife.195...
Data
We include source data for Figures 6, 7 and 8 concerning reproducibility results of both Eco-HAB measures.The names of the Excel sheetsrefer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.7554/eLife.195...
Data
We include source data for Figures 6, 7 and 8 concerning reproducibility results of both Eco-HAB measures.The names of the Excel sheetsrefer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.7554/eLife.195...
Data
Raw data from U-tube dominance test and Eco-HAB measured activity (number of visits to all compartments of the apparatus during 1st 12-hr period of adaptation).Figure 9 depicts correlation between those two variables.DOI: http://dx.doi.org/10.7554/eLife.19532.035
Data
We include source data for Figures 6, 7 and 8 concerning reproducibility results of both Eco-HAB measures.The names of the Excel sheetsrefer to corresponding figures and contain data used for analysis of the behavioral measures obtained by the implementation of Eco-HAB.py software (see Materials and methods).DOI: http://dx.doi.org/10.7554/eLife.195...
Data
Eco-HAB.py scripts with sample data enabling their execution. DOI: http://dx.doi.org/10.7554/eLife.19532.038
Data
Raw data from U-tube dominance test and Eco-HAB measured activity (number of visits to all compartments of the apparatus during 1st 12 hr period of adaptation).Figure 9 depicts correlation between those two variables.DOI: http://dx.doi.org/10.7554/eLife.19532.036
Article
Full-text available
Eco-HAB is an open source, RFID-based system for automated measurement and analysis of social preference and in-cohort sociability in mice. The system closely follows murine ethology. It requires no contact between a human experimenter and tested animals, overcoming the confounding factors that lead to irreproducible assessment of murine social beh...
Article
A number of studies have identified the importance of dopaminergic, opioid, serotonergic, noradrenergic and glutamatergic neurotransmission in amphetamine-induced “50-kHz” ultrasonic vocalizations (USVs). Amphetamine became a topic of interest for many researchers interested in USVs due to its ability to induce 50-kHz USVs. To date, it has been dif...
Article
In the present study, we investigated the effect of ACPT-I [(1S, 3R,4S)-1-aminocyclopentane-1,2,4-tricarboxylic acid], a blood-brain-barrier permeable agonist of group III mGlu receptor, against oxygen-glucose deprivation (OGD)-evoked neuronal cell death in primary neuronal cell cultures and in the model of transient middle cerebral artery occlusio...
Article
Parkinsonism is underlain by dopamine (DA) deficiency in the mid-brain, a neurotransmitter innately involved with respiratory regulation. However, the state of respiration in parkinsonism is an unsettled issue. In this study we seek to determine ventilation and its responses to hypoxia in a reserpine - alpha-methyl-tyrosine model of parkinsonism in...
Article
Full-text available
Previous studies have demonstrated that repeated submission of rats to mild hypobaric hypoxia reduces the persistent behavioral and hormonal depressive symptoms induced by exposure to footshock in the learned helplessness paradigm. The aim of this study was to determine whether hypoxic preconditioning of mice can also induce antidepressant- and anx...
Conference Paper
Full-text available
Objectives: The objective of our study was to evaluate various features of olfactory functioning in old (1 or 1.5 year) mice in two time points after MPTP intoxication (1 week and 24 weeks). Methods: The olfactory capability in mice after acute injection of MPTP was evaluated using a set of behavioral tests: 1) The Buried Food Test (BFT) to evalu...
Article
Persistent-firing neurons in the entorhinal cortex (EC) and the lateral nucleus of the amygdala (LA) continue to discharge long after the termination of the original, spike-initiating current. An emerging theory proposes that endogenous persistent firing helps support a transient memory system. This study demonstrated that persistent-firing neurons...
Article
A growing body of data indicates that changes in emotional behavior occur with age. Young Lewis rats are known to display hypofunction of the HPA axis. With age the reactivity of this axis is thought to increase with a concomitant rise in anxiety. In the current study, we investigate how and if the pattern of neuronal activation (measured as c-Fos...
Article
Full-text available
Psychogenetically selected Roman high (RHA/Verh) and Roman low (RLA/Verh) avoidance rats constitute a well-recognized model of diverse emotional reactivity. The two Swiss lines display marked behavioral and endocrine differences in reaction to a novel environment. In our study we found that these differences are accompanied by a distinct, line-spec...
Article
The hippocampus is well-known to be critical for trace fear conditioning, but nothing is known about the importance of perirhinal cortex (PR), which has reciprocal connections with hippocampus. PR damage severely impairs delay fear conditioning to ultrasonic vocalizations (USVs) and discontinuous tones (pips), but has no effect on delay conditionin...
Article
Rodent ultrasonic vocalizations (USVs) are ethologically critical social signals. Rats emit 22kHz USVs and 50kHz USVs, respectively, in conjunction with negative and positive affective states. Little is known about what controls emotional reactivity to these social signals. Using male Sprague-Dawley rats, we examined unconditional and conditional f...
Article
Recent studies have reported an age-related increase of anxiety in rodents with a concomitant decrease in neuronal activity in some of the key structures of the fear/anxiety circuit. In the present study we present evidence that distinct parts of this circuit are differentially affected by age in Lewis rats. The effect of ageing is observed both at...
Article
Full-text available
Emotional states displayed by an animal or a human can seriously affect behavior of their conspecifics. The amygdala plays a crucial role in the processing of emotions. In this study, we describe an experimental rat model of between-subject transfer of emotional information and its effects on activation of the amygdala. The rats were kept in pairs,...
Article
Full-text available
During aging many of the brain functions became deteriorated or altered. One of the most important age related changes is an increase of anxiety level, reported both in humans and in animals. Our study was intended to compare c-fos gene expression in amygdala, the key structure in anxiety/fear regulation, in old (24 months) and young (4 months) rat...
Article
The present study investigated age-related differences in the emotional behavior of rats using factor analysis to identify motivational factors influencing spontaneous behavior in open field with illuminated center (OF), plus maze (EPM) and social interactions test. Animals of the same strain, bred under the same conditions, formed two experimental...
Article
Full-text available
The effects of bicuculline methiodide administration into ventromedial hypothalamus (15 ng per site, bilaterally) on fear behavior and monoamines (NA, DA, 5-HT) and GABA in structures of the brain defensive system (hypothalamus, midbrain gray matter, amygdala, hippocampus and frontal cortex) were studied. Fear behavior was examined in the modified...

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