Pawan Kumar

Pawan Kumar
Lala Lajpat Rai University of Veterinary and Animal Sciences · Veterinary Anatomy

BVSc & AH, MVSc, Ph.D., PDF

About

98
Publications
4,026
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681
Citations
Additional affiliations
January 2003 - July 2004
University of Kentucky
Position
  • Post Doctorate Fellow
May 1999 - May 2000
University of Kentucky
Position
  • Post Doctorate Research Fellow
Education
May 1999 - April 2000
University of Kentucky
Field of study
  • Vetrerinary Anatomy
January 1991 - March 1994
CCS Haryana Agricultural University
Field of study
  • Vetrerinary Anatomy
July 1984 - January 1989
CCS Haryana Agricultural University
Field of study
  • Veterinary Sciences

Publications

Publications (98)
Article
Full-text available
The present work was undertaken on 10 pigs to study the gross anatomy of the nasal turbinates. The nasal cavity in pig was divided into twocompartments by median nasal septum. The dorsal nasal turbinate was the largest turbinate whichextended from the dorsal part of ethmoidal crest of ethmoid bone to the level of first transverse ruga of hard palat...
Article
Full-text available
The present study was conducted on 18 young pigs of 8-10 months age of local mixed breed to determine the anatomical features of nasal commissures and alae. The gross anatomical studies revealed that external nares were round to oval in shape and bounded by dorsal and ventral commissures and medial and lateral alae. The histological studies reveale...
Article
Full-text available
Surface characteristics of the nasal epithelium were studied in six young pigs by the scanning electron microscope. Samples were taken from various parts of nasal cavity and prepared for scanning electron microscopic study in a routine manner. The nasal cavity presented five regions under electron microscope. The vestibular region presented flat le...
Article
Full-text available
The vomeronasal organ present at the ventral aspect of median nasal septum opened rostrally at lateral margins of incisive papilla and caudally, it extended into a blind end at the level of sixth transverse ruga. A hyaline type of cartilage incompletely surrounded the vomeronasal organ. The vomeronasal organ was lined by stratified squamous non-ker...
Article
Full-text available
Six heads of young pigs of 8-10 months age, of local mixed breed pigs were used to study the histology and histochemistry of the larynx. Larynx was comprised of epiglottis, paired arytenoid, thyroid and cricoid cartilages. Histologically the epiglottis, inter-arytenoid, arytenoid cartilages and vocal cords were lined by stratified squamous non-kera...
Article
Full-text available
The present study was conducted on six local mixed breed pigs of 8-10 months age to study the histology and histochemistry of the nasal cavity. Histologically the nasal cavity was lined with three different types of epithelium i.e. stratified squamous, pseudostratified ciliated columnar and olfactory epithelium lining the vestibular, respiratory an...
Article
Full-text available
The present study was conducted on 12 young pigs of 8-10 months age of local mixed breed to determine the structural features of the ethmoturbinates. Major portion of the ethmoturbinates was lined by pseudostratified columnar ciliated epithelium with PAS positive goblet cells. The caudal portion of the ethmoturbinates adjacent to the cribriform pla...
Article
Full-text available
The ileum was collected from five adult sheep immediately after slaughter. The ileal tissues were fixed in 10% neutral buffered formalin and processed for light microscopy. The villi of the ileum varied from elongated to blunt with broad bases and were lined by a simple columnar epithelium having few goblet cells. The intestinal glands or crypts of...
Article
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The present study was planned to study the histology and histochemistry of the lacrimal gland of six healthy young goats of local mixed breed. The gland was located at the dorsolateral aspect of the eyeball. The lacrimal gland of goat characterized as compound tubulo-acinar type was surrounded by a thin layer of connective tissue which penetrated i...
Article
Full-text available
The present work was carried out on the gross anatomical and scanning electron microscopic studies in the larynx of 12 non-descript pigs of 8- 12 months of age. Gross anatomically, the larynx in pig was a short tubular structure supported by unpaired epiglottis, thyroid and cricoid cartilages and paired arytenoids cartilage. Epiglottis was charact...
Article
Full-text available
The present study was conducted on six healthy young sheep of local mixed breed of either sex to study the histology and histochemistry of the lacrimal gland. The compound tubulo-acinar gland was consisting of serous, mucous and sero-mucous secretory units. Myoepithelial cells were located at the basal part of secretory end pieces and formed the in...
Article
Full-text available
Mesenchymal stem cells (MSCs) are promising therapeutic tools for the treatment of tendon rupture and other musculoskeletal injuries in horses. Although MSCs from bone marrow and adipose tissues are commonly used for therapeutic purpose in equines, umbilical cord blood (UCB) and amniotic fluid (AF) are potential non-invasive sources of MSCs. We col...
Research
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SEM of cricoid cartilage. Interesting patterns. Is someone working onthe same aspect?
Research
Full-text available
SEM of thyroid cartilage. Any body having knowledge about this.
Research
Full-text available
SEM of arytenoid cartilage. is there anybody who is having similar type of pictures?
Article
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Mesenchymal stem cells (MSCs) offer promise as therapeutic aid in the repair of tendon and ligament injuries in race horses. Fetal adnexa is considered as an ideal source of MSCs due to many advantages, including non-invasive nature of isolation procedures and availability of large tissue mass for harvesting the cells. However, MSCs isolated from e...
Article
Full-text available
The tissues from the buccal and labial salivary glands of 25 healthy adult sheep of local mixed breed of either sex were collected and processed for paraffin and frozen sectioning techniques. Small intralobular ducts were lined by simple cuboidal type of epithelium whereas, large intralobular ducts were lined by simple cuboidal to simple columnar t...
Article
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The present study was undertaken on ten healthy adult sheep of local mixed breed of either sex to study the histo-morphological, histochemical and micrometrical studies on the labial salivary glands of sheep. The labial salivary glands were of branched tubuloacinar type having loose irregular connective tissue and surrounded by myoepithelial cells....
Data
Full-text available
Article
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The present study was conducted on jejunum of five adult sheep collected from an abattoir. The tissues were fixed in 10% neutral buffered formalin and processed. Histological studies revealed that the villi varied from elongated pointed to broad base and were lined by simple columnar epithelium with few goblet cells. The intestinal glands were simp...
Article
Full-text available
Tendon injuries are common in race horses, and mesenchymal stem cells (MSCs) isolated from adult and foetal tissue have been used for tendon regeneration. In the present study, we evaluated equine amniotic fluid (AF) as a source of MSCs and standardised methodology and markers for their in vitro tenogenic differentiation. Plastic-adherent colonies...
Article
Mesenchymal stem cells (MSCs) isolated from umbilical cord blood (UCB) in equines have not been well characterized with respect to the expression of pluripotency and mesenchymal markers and for tenogenic differentiation potential in vitro. The plastic adherent fibroblast-like cells isolated from 13 out of 20 UCB samples could proliferate till passa...
Article
The dorsal and ventral walls of rumen of goat studded with papillae of varying shapes and dimensions were lined by stratified squamous keratinized epithelium having varying number of rows in strata basale, spinosum, granulosum and corneum. Iron granules were observed in superficial layers of stratum corneum. The propria submucosa having loose irreg...
Article
Full-text available
The regeneration of lost tissue is known to mankind for several years, but it is only in the recent past that research on cell-therapies has gained momentum and eluded the dramatic yet scientific advancements in the field of regenerative medicine. Stem cells have gained increased attention in human as well as veterinary biomedical research due to t...
Article
The haematological and biochemical profile of equine umbilical cord blood (UCB) is not well known presently and very little information is available about peripheral blood of equine neonates immediately after foaling. This information has relevance as UCB is a rich source of progenitor stem cells and neonatal hematology may be useful in early disea...
Article
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The umbilical cords collected at the time of foaling of Thoroughbred mares presented tortuous course due to curving of umbilical arteries and veins and measured 55-65 cm from ligation site of foal to the attachment of placenta. Formalin-fixed tissues of umbilical cord having artery, vein and urachus were processed to make 5-6 μ paraffin sections. T...
Article
Full-text available
The histomorphology and histochemistry of the ventral buccal gland were studied in ten healthy adult sheep of local mixed breed of either sex. Histologically, it was a compound tubulo-alveolar gland having sero-mucous nature with predominance of serous alveoli. Histochemically, the mucous as well as serous alveoli of this gland exhibited negative r...
Article
Full-text available
The present histological study conducted on umbilical cords of early age buffalo foetii revealed two thick walled arteries, two thin walled veins and an allantoic duct being surrounded by mesenchymal connective tissue.The preipherally placed amnion presented small papillae containing glycogen. The advancement of foetal age led to an increase in con...
Article
Full-text available
The present study was conducted on the dorsal buccal gland of ten healthy adult sheep of local mixed breed. Histologically, the gland was compound tubulo-alveolar being surrounded by less distinct capsule. The gland was sero-mucous with predominance of mucous alveoli. The mucous alveoli showed positive reaction to mucosubstances, glycogen, mucopoly...
Article
Full-text available
The tissues from the middle buccal gland of ten healthy adult sheep of local mixed breed of either sex were collected from rostral, middle and caudal regions and processed for paraffin and frozen sectioning techniques. The middle buccal gland was extended infront of the masseteric groove from the point of intersection of transverse facial artery wi...
Article
Full-text available
Ten adult sheep of local mixed breed of either sex were selected to study the topographic anatomy of buccal and labial glands. The buccal glands of sheep were arranged in three rows viz. dorsal, middle and ventral. The dorsal buccal gland was distributed over the buccinator muscle, in front of the masseter muscle and masseteric groove below the dep...
Article
Full-text available
Infection by Leptospira interrogans has been causally associated with human and equine uveitis. Studies in our laboratories have demonstrated that leptospiral lipoprotein LruA and LruB are expressed in the eyes of uveitic horses, and that antibodies directed against LruA and LruB react with equine lenticular and retinal extracts, respectively. Thes...
Article
The light and electron microscopy was conducted on Reissner's fibre (RF) in 6 young horses of either sex. RF representing cilia, microvilli along with heterogeneous material consisting of RBC and cell debris lay in mid line towards the ventricular surface of the subcommissural organ (SCO) ependyma and cerebral aqueduct. The SEM revealed Reissner's...
Article
Little is known about entry and subsequent multiplication of Streptococcus equi following exposure of a susceptible horse. This information would have value in design of intranasal vaccines and understanding of shedding and protective immune responses. To determine entry points and sites of subsequent replication and dispersion of S. equi at differ...
Article
The present study conducted on 6 young (10-12 months of age) horses of either sex revealed that subcommissural organ was present on ventral aspect of posterior commissure and extended from pineal gland to recessus mesocoelicus forming roof of III ventricle of the brain and partly of cerebral aqueduct. The organ was fully developed and comprised an...
Article
Scanning electron-microscopy of the nasal cavity was performed on 5 male healthy buffalo calves. The nasal cavity was divided rostro-caudally into vestibular, transitional-I, respiratory, transitional-II and olfactory regions. The vestibular portion was lined by stratified squamous epithelium with polyangular cells forming island-like structures. T...
Article
The scanning electron microscopy revealed 5 zones in the nasal cavity. The rostral most vestibular zone was lined by stratified squamous non-keratinized epithelium having flat leaf like cells, which presented microplicae at higher magnification. Transitional zone-I represented a transition from stratified squamous to respiratory epithelium. The lar...
Article
Scanning electron microscopy of the mechanical and gustatory lingual papillae of 5 young horses was investigated. Numerous conical filiform papillae on the dorsum of the tongue had a single projection toward their free tips and a lateral serrated border. Similar papillae on the ventro-lateral surface were smaller and sparsely distributed. Round to...
Article
The tonsil of the soft palate and the lingual tonsils were the chief entry points for Streptococcus equi (Se), the cause of equine strangles, in intranasally inoculated horses. Microplicae on the stratified squamous epithelial cells were important in initial attachment. Within a few hours, small numbers of Se were present in the tonsillar crypts, l...
Article
The tonsil of the soft palate was an oval, flat structure located centro-rostrally on the oral surface of the soft palate. Its stratified squamous non-keratinized epithelium was perforated by holes or small crypts the deeper parts of which were loosely spongiform inter-digitated with lymphoid tissue. These unusual features have not previously been...
Article
Four Quarter Horses (9 months to 7 years of age) with submandibular lymphadenopathy and firm muscles (palpation of which elicited signs of pain) were evaluated; in general, the horses had a stiff gait, and 3 horses became recumbent. Streptococcus equi was cultured from aspirates of lymph nodes or samples of purulent material collected from the audi...
Article
The tubal tonsil of the horse surrounds the pharyngeal opening of the eustachian tube and is lined by pseudostratified columnar ciliated epithelium interspersed with areas of follicle-associated epithelium (FAE) heavily infiltrated by lymphocytes but devoid of goblet and ciliated cells. Scanning and transmission electron microscopy revealed microvi...
Article
The palatine tonsils of five young horses formed 10-12 cm elongated follicular structures extending from the root of the tongue on either side to the base of the epiglottis and lateral to the glossoepiglottic fold. The stratified squamous non-keratinized epithelium of the outer surface was modified into crypts as reticular epithelium by heavy infil...
Article
The stratified squamous epithelium of the lingual tonsil of five young horses was infiltrated with CD4 and CD8 positive cells, which were very numerous in the crypt reticular epithelium along with macrophages and IgGb and IgA positive cells. Lymphoid follicles of the lamina propria mucosae consisted of a parafollicular area, corona and germinal cen...
Article
The microstructural and ultrastructural features of the equine lingual tonsil were studied in five young horses. Located at the root of the tongue it presented an irregular surface with rounded elevations, numerous folds and crypts. Stratified squamous non-keratinized epithelium lining its outer surface was modified by heavy infiltration of lymphoi...
Article
The subcommissural organ of sheep structurally had ependyma, subependymal glial zone and hypendyma. Pseudostratified columnar ciliated ependyma showed 3 types of cells. Basal cytoplasmic processes of ependymal cells mainly constituted subependymal glia zone along with blood capillaries, nerve fibres and glia cells. Hypendymal cells were classified...
Article
The scanning electron microscopy of the subcommissural organ of sheep revealed apical spherical protrusions and ciliary network. Cilia emerged from these protrusions and intermixed with others. Smooth surfaced spherical protrusions marked the presence of non-ciliated cells. The secretions of the subcommissural organ along with RBCs and cell debris...
Article
The aim of this study was to characterise the morphological and histochemical features of equine nasopharyngeal tonsillar tissue. Nasal and oropharyngeal tonsillar tissue has been described as the gatekeeper to mucosal immunity because of its strategic location at the entrance to the respiratory and alimentary tracts. A combination of light, scanni...
Article
Light and electron microscope studies were conducted on the nasopharynx and the nasopharyngeal tonsil of 15 young horses. The nasopharynx and nasopharyngeal tonsil was lined with pseudostratified columnar ciliated epithelium and goblet cells. The lymphoepithelium of the nasopharyngeal tonsil was folded forming crypts, the mucosa of which was modifi...
Article
The hypendyma of subcommissural organ was present below the ependyma just adjacent to posterior commissure. The hypendymal cells were classified into 2 types on the basis of nuclear morphology. Irregular shaped type-I cells nuclei possessed small aggregates of chromatin and nucleolus in the form of chromoplast. Type-II cells nuclei had nuclear clef...
Article
Micrometric observations were made on the spleens of 8 camels at 8 different regions. Various parameters studied were mean, S.D., S.E. and Correlation coefficient of capsule thickness, trabecular thickness, mean diameter of follicle, number of follicles per cm2 area, diameter of smallest and largest follicles, % trabecular, white and red pulp areas...
Article
The cells of pseudostratified columnar ciliated ependyma of the subcommissural organ in the goat were classified into two types on the basis of the distribution of chromatin material and nuclear clefts. Amongst the cell organelles the endoplasmic reticulum was highly developed and formed three types of Nebenkerne systems. The type-I Nebenkerne had...
Article
The nasal turbinates of 5 young horses were studied by light and scanning electron-microscopy. Stratified cuboidal epithelium lined the rostral part of the dorsal and ventral nasal turbinates of the vestibular region. The polyangular microvillus cells of this region were separated by linear depressions. The mid and caudal parts of the dorsal and ve...
Article
The subcommissural organ studied in five adult camels was located on the ventral aspect of caudal commissure and extended from the pineal gland to recessus mesocoelicus forming mainly the roof of III ventricle. It was comprised of a pseudostratified columnar ciliated ependyma towards the ventricular surface which later changed abruptly to simple ep...
Article
Nasal cavity of goats presented 5 zones under scanning electron microscope: 1. the vestibular zone had stratified squamous non-keratinized epithelium; 2. the transitional zone-I was a transformation from stratified squamous to respiratory epithelium; 3. the respiratory zone presented ciliated, nonciliated and goblet cells; 4. the transitional zone-...
Article
The tongue papillae of 6-9-month-old Jamunapari goats were studied by scanning electron-microscopy. The conical-shaped filiform papillae had 3-6 pointed projections and 6-8 secondary papillae at the free tip and the base of the dorsal surfaced of the tongue, respectively. The convex surfaced fungiform papillae were raised above the lingual mucosa....
Article
The study was conducted on 10 Jamunapari young male goats (6-9 months age) to explore the structure and extent of Reissner's fibre. The Reissner's fibre was present on ependyma of subcommissural organ and cerebral aqueduct which was constituted of cilia, microvilli, glycocalyx along with heterogeneous material. Scanning electron-microscopy showed f...
Article
The dorsal surface of tongue in 6 adult camels (Camelus dromedarius) was studied by scanning electron microscopy to establish morphostructural characteristics of mechanical and gustatory papillae. The conical shaped filiform papilla with single projection presented crater like depression in its basal portion. A shallow groove separated the fungifor...
Article
The histomorphology of subcommissural organ was studied in male goats (6-9 months age). A pseudostratified columnar ciliated ependyma lining the organ showed 6-10 rows of type-I and II cells. The subependymal glial zone contained basal cytoplasmic processes of ependymal cells, blood capillaries and few glial cells. The hypendyma had type-I and II h...
Article
The III ventricle roof of goat brain in its mid portion presented synchronous wave like dense arrangement of cilia. However, its extension towards pineal recess had transitional zone with the predominance of micro-villi, supraependymal cells and neurons of different shapes. The cilia of lateral ventricle were arranged star like whereas, those of IV...
Article
Histologically different enzymes were studied in subcommissural organ of 10 young male goats of local mixed breed. Acid phosphatase as diffused homogeneous activity was localized throughout the ependyma and blood capillaries whereas, alkaline phosphatase, glucose-6-phosphatase, succinic dehydrogenase and cytochrome oxidase were mainly distributed a...
Article
Scanning electron-microscopy demonstrated irregular meshwork of fibrous strands on the capsular surface of goat pineal gland, whereas, the cut surface of the gland displayed cauliflower like arrangement of pinealocytes. At the ultrastructural level the capsule contained light and dark cells. Pinealocytes located in the parenchyma presented comparat...
Article
Male goats (10) of 6-9 months age group were used. The neutral lipids were found uniformly distributed whereas, the bound lipids presented more tendency towards the base of ependyma. The neutral mycopolysaccharides were more concentrated at inter-cellular junctions and vacuolated areas were devoid of PAS staining. The acidic mucopolysaccharides and...
Article
The olfactory and respiratory mucosal linings of ethmoturbinate surfaces were studied histologically at different levels in ten goats. The major area of ethmoturbinates was lined with respiratory mucosa and only a small strip measuring 2.5–3.0 mm adjacent to the cribriform plate of the ethmoid had lining of true olfactory mucosa. The pseudostratifi...
Article
Full-text available
The subcommissural organ of pig extended from pineal gland to recessus mesocoelicus on the ventral aspect of posterior commissure and formed partly the roof of third ventricle and cerebral aqueduct. The organ was comprised of two cellular layers i.e. a pseudostratified columnar ciliated ependyma towards the free ventricular surface and a hypendyma...

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Project (1)
Archived project
I Standardization of immunohistochemical techniques for diagnosis of important infectious diseases and tumour conditions prevalent in the state of Haryana. II. Standardization of immunohistochemical techniques on healthy organs for comparative purpose. III. Analysis of tissue samples employing special standard histochemical staining procedures. IV. Upgradation and modernization of the Immunotechnology laboratory. V. Maintenance of Phage Display Library and characterization of already isolated and selected single domain antibody clones from the Phage Display Library