Pavla Pecherkova

• Institute of Haematology and Blood Transfusion

In this study, we conducted a comprehensive survey involving a substantial sample size (n = 6,405) of urban daily commuters across four European nations (Germany, the United Kingdom, Poland, and the Czech Republic). Our investigation contributes to an enriched comprehension of the user dynamics associated with Mobility-as-a-Service alternatives and...

Background: Pediatric-inspired protocols with prospective monitoring of minimal residual disease (MRD) are considered the standard of intensive treatment for adults with acute lymphoblastic leukemia (ALL). They have been used in the Czech Republic since 2007. Patients and methods: Two hundred and ninety-seven patients aged 18-65 years were treat...

Tisagenlecleucel (tisa-cel) is a CD19-specific CAR-T cell product approved for the treatment of relapsed/refractory (r/r) DLBCL or B-ALL. We have followed a group of patients diagnosed with childhood B-ALL (n = 5), adult B-ALL (n = 2), and DLBCL (n = 25) who were treated with tisa-cel under non-clinical trial conditions. The goal was to determine h...

In this study we conduct a large survey (n=6,405) on urban daily commuters in four European countries (Germany, the United Kingdom, Poland, and the Czech Republic), we add richness to our understanding of who will use Mobility-as-a-Service (MaaS) options and what relationship MaaS has with public transit in terms of choosing how to travel. We explo...

In response to society’s volatile and changing mobility requirements, many new mobility concepts and business models are currently being developed and piloted. The Mobility-as-a-Service (MaaS) concept attempts to meet rising customer demands in a needs-based and situational manner. For the success of innovative mobility offers, user acceptance and...

With the constant and ever-growing pressure from governing bodies for increased sustainability of transportation systems, the need for changes in the travel behaviours of the inhabitants of suburban and urban areas seems inevitable. Thus a shift from cars to vehicles with higher occupancy rates or other more environmental friendly travel modes need...

The global Covid-19 pandemic has had a great impact on the air transport network (ATN) and on aviation in general. April 2020 saw the largest decline in connectivity, with an average drop of more than 80% compared to 2019. This paper investigates this decline in terms of centrality measures. To capture the first wave of the pandemic, i.e., the larg...

Urban mobility is currently facing a variety of challenges. Increasing emission levels in cities, growing numbers of private vehicles and constantly changing needs and requirements of the population are only some of them. New mobility concepts and business models are currently emerging in response to these volatile conditions, primarily through imp...

Background: Leucine-rich alpha-2-glycoprotein (LRG) has been repeatedly proposed as a potential plasma biomarker for myelodysplastic syndrome (MDS). Objective: The goal of our work was to establish the total LRG plasma level and LRG posttranslational modifications (PTMs) as a suitable MDS biomarker. Methods: The total plasma LRG concentration...

Background Extracellular vesicles are released into body fluids from the majority of, if not all, cell types. Because their secretion and specific cargo (e.g., proteins) varies according to pathology, extracellular vesicles may prove a rich source of biomarkers. However, their biological and pathophysiological functions are poorly understood in hem...

Functional diploid Acipenser ruthenus, functional tetraploid Acipenser gueldenstaedtii and functional hexaploid Acipenser brevirostrum juveniles were sampled monthly for one year, and the white blood cell indicators were determined. The total number of leukocytes (TL) was 40.93 ± 17.24 × 10⁹/l for the diploids, 20.63 ± 11.20 × 10<sup>9</...

The aim of the present study was to evaluate the significance of low‐level minimal/measurable residual disease (MRD) during early consolidation treatment in adult BCR‐ABL1–negative acute lymphoblastic leukaemia. The MRD load was monitored by immunoglobulin/T‐cell receptor rearrangements and assessed as negative [complete MRD response (CMR)], positi...

BK polyomavirus (BKPyV) persists lifelong in renal and urothelial cells with asymptomatic urinary shedding in healthy individuals. In some immunocompromised persons after transplantation of hematopoietic stem cells (HSCT), the BKPyV high-rate replication is associated with haemorrhagic cystitis (HC). We tested whether the status of BKPyV immunity p...

BK polyomavirus (BKV) persists lifelong in the urinary tract with asymptomatic urinary shedding in healthy individuals. In immunocompromised persons after transplantation of hematopoietic stem cells (HSCT) the BKV high-rate replication is associated with haemorrhagic cystitis (HC) with a reported incidence of 17 %. Numerous studies of reconstitutio...

Carsharing is a relatively new and growing mobility service in many cities. Although there are numerous carsharing initiatives in operation around the world, it is still not clear how they will affect overall travel behavior. Understanding the effects of carsharing when combined with other existing travel modes is an important prerequisite for deci...

Background: myelodysplastic syndrome (MDS) is a hematopoietic stem cell disorder with an incompletely known pathogenesis. Long noncoding RNAs (lncRNAs) play multiple roles in hematopoiesis and represent a new class of biomarkers and therapeutic targets, but information on their roles in MDS is limited. Aims: here, we aimed to characterize lncRNA...

This work investigated patient-specific genomic BCR-ABL1 fusions as markers of measurable residual disease (MRD) in chronic myeloid leukaemia, with a focus on relevance to treatment-free remission (TFR) after achievement of deep molecular response (DMR) on tyrosine kinase inhibitor (TKI) therapy. DNA and mRNA BCR-ABL1 measurements by qPCR were comp...

Myelodysplastic syndromes (MDS) are hematopoietic stem cell disorders with large heterogeneity at the clinical and molecular levels. As diagnostic procedures shift from bone marrow biopsies towards less invasive techniques, circulating small noncoding RNAs (sncRNAs) have become of particular interest as potential novel noninvasive biomarkers of the...

Background Treatment free remission (TFR) or molecular relapse free survival (MRFS) have become important therapeutic aims for patients with chronic myeloid leukemia (CML). This aim can be realised through achievement of a sustained deep molecular response (DMR). Aims In our previous work (Machova Polakova K, EHA 2018) we showed that a BCR‐ABL1 DN...

Background: Aberrant epigenetic patterns are a hallmark of acute myeloid leukemia (AML). Mutations in profound epigenetic regulators DNMT3A and IDH1/2 often occur concurrently in AML. Objectives: The aim was to analyze DNA methylation, hydroxymethylation and mRNA expression profiles in AML with mutations in DNMT3A and IDH1/2 (individually and in...

Acute myeloid leukemia with mutated nucleophosmin (NPMc+ AML) forms a distinct AML subgroup with better prognosis which can potentially be associated with immune response against the mutated nucleophosmin (NPM). As the T-cell-mediated immunity involves antigen presentation on HLA class I molecules, we hypothesized that individuals with suitable HLA...

Raw data for multivariate analysis. (XLSX)

Introduction: In chronic myeloid leukemia (CML) resistant to tyrosine kinase inhibitors (TKI), detection of mutations in the BCR-ABL1 kinase domain (KD) is routinely performed on transcript level. To determine the level of BCR-ABL1 KD mutation is important to follow kinetics of resistant CML cells and therapeutically prevent progression. However, t...

INTRODUCTION: Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematopoietic stem cell (HSC) disorders characterized by dysplastic and ineffective blood cell production and a tendency for transformation to acute myeloid leukemia (AML). Because of the heterogeneity of MDS, the development of additional molecular tools able to refi...

Acute myeloid leukemia with mutated nucleophosmin (NPMc+ AML) forms a distinct AML subgroup with better prognosis which can potentially be associated with immune response against the mutated nucleophosmin (NPM). As the T-cell-mediated immunity involves antigen presentation on HLA class I molecules, we hypothesized that individuals with suitable HLA...

The fusion oncoprotein BCR-ABL1 exhibits aberrant tyrosine kinase activity and has been proposed to deregulate signaling networks involving both transcription factors and non-coding microRNAs that result in chronic myeloid leukemia. Previously, microRNA expression profiling showed deregulated expression of miR-150 and miR-155 in chronic myeloid leu...

Background: We retrospectively analyzed data from 310 patients with acute myeloid leukemia with intermediate-risk cytogenetics in first complete remission (CR1) to evaluate the usage and efficacy of various types of postremission therapy. Patients and methods: Cox regression with time-dependent covariates, landmark analysis, and competing risk m...

Background: The expression of miRNAs is regulated at transcriptional and posttranscriptional levels. Dysregulation of miRNAs could directly induce or be a consequence of oncogenic pathways. Chronic myeloid leukemia (CML) is characterized by reduced miR-150 levels leading to an insufficient repression of its target, oncogene MYB. CML treatment with...

Background Through high-throughput next-generation sequencing of promoters of solute carrier and ATP-binding cassette genes, which encode drug transporters, we aimed to identify SNPs associated with the response to imatinib administered for first-line treatment of patients with chronic myeloid leukemia. Methods In silico analysis using publicly ava...

Our work examined the production of intracellular interferon (INF)-?, tumor necrosis factor (TNF)-?, interleukin (IL)-2, and IL-4 by in vitro stimulated CD3+ cells from 38 chronic myeloid leukemia (CML) patients. At the time of diagnosis the percentages of cells producing INF-?, TNF-?, and IL-2 were strongly suppressed compared to those in healthy...

The aim of our study was to analyze the distribution of HLA-DQB1 in Czech patients with central hypersomnias and differences between diagnostic groups of narcolepsy type 1 (NT1), type 2 (NT2), idiopathic hypersomnia (IH) and no central hypersomnia subjects (no-CH). Statistical analysis of DQB1 genotyping was performed on the cohort of 716 patients...

This paper deals with problem of analysis of traffic data. A traffic network has several types of roads: historical centre, peripherals, arterial roads, etc. They have specific properties. For a traffic analysis, large amounts of data are needed. Some traffic data are difficult to obtain due to their rare occurrence. Typical example is the investig...

The paper deals with detection of clusters in data measured on a driven vehicle. Such a clustering aims at distinguishing various driving styles for eco-driving and driver assistance systems. The task is solved with the help of the application of the recursive Bayesian mixture estimation theory. The main contribution of the paper is a demonstration...

This chapter deals with the model design of traffic systems. The main aims were to introduce the modelling technique and to present validation methods for the designed models. The proposed modelling technique is based on the traffic flow conservation principle. The use of the technique was illustrated on three simple common micro-regions that can c...

The paper deals with the application of the state and parameter estimation techniques in the area of traffic control. The most important properties of the traffic system are described and the model of the traffic system, based on the traffic flow conservation principle, is presented. Various estimation and identification techniques are briefly intr...

This paper deals with the problem of traffic flow modelling and state estimation for historical urban areas. The most important properties of the traffic system are described. Then the model of the traffic system is presented. The weakness of the model is pointed out and subsequently rectified. Various estimation and identification techniques, used...

Success/failure of adaptive control algorithmsespecially those designed using Linear Quadratic Gaussian criteriondepends on the quality of the process data used for model identication. One of the most harmful types of process data corruptions are outliers, i.ewrong data' lying far away from the range of real data. The presence of outliers in the da...

This paper deals with modelling and estimation tasks in the traffic control problem. A special case is considered where the intersection arms in the traffic systems are equipped with detectors and the traffic control is based on the minimisation of the total lengths of queues in the system. However, the detectors directly measure the intensity and...

The estimation of queue lengths via local lters is depend on initial settings of covariances and state parameters if they are unknown. In the trac, it is possible used the old measurements for initial setting. In the trac data, the uncertainties are and the noise is not correlated and so it is not suitable using the method of least squares. Using o...

In many big cities, trac congestion extends far beyond city boundaries. Prague, the capitol of the Czech Republic, is no exception to the rule. The people are willing to pay a high cost commuting by cars (time, travel cost, increased possibility of accidents...). The the building of new streets is not usually possible in cities with the historical...