Pavel Němec

Charles University in Prague | CUNI · Department of Zoology, Faculty of Science

Significance Birds are remarkably intelligent, although their brains are small. Corvids and some parrots are capable of cognitive feats comparable to those of great apes. How do birds achieve impressive cognitive prowess with walnut-sized brains? We investigated the cellular composition of the brains of 28 avian species, uncovering a straightforwar...

The social brain hypothesis (SBH) posits that the demands imposed on individuals by living in cohesive social groups exert a selection pressure favouring the evolution of large brains and complex cognitive abilities. Using volumetry and the isotropic fractionator to determine the size of and numbers of neurons in specific brain regions, here we tes...

Neurons are the basic computational units of the brain, but brain size is the predominant surrogate measure of brain functional capacity in comparative and cognitive neuroscience. This approach is based on the assumption that larger brains harbour higher numbers of neurons and their connections and therefore have a higher information‐processing cap...

Significance The evolution of brain processing capacity has traditionally been inferred from data on brain size. However, similarly sized brains of distantly related species can differ in the number and distribution of neurons, their basic computational units. Therefore, a finer-grained approach is needed to reveal the evolutionary paths to increas...

A longstanding issue in biology is whether the intelligence of animals can be predicted by absolute or relative brain size. However, progress has been hampered by an insufficient understanding of how neuron numbers shape internal brain organization and cognitive performance. On the basis of estimations of neuron numbers for 111 bird species, we sho...

Smell abilities differ greatly among vertebrate species, as reflected by the exceptional size variability of gene families and brain regions involved in odour detection. However, key environmental factors shaping the molecular and phenotypic evolution of the olfactory sensory system remain mostly unknown. Here, we investigate the association betwee...

Visual (and probably also magnetic) signal processing starts at the first synapse, at which photoreceptors contact different types of bipolar cells, thereby feeding information into different processing channels. In the chicken retina, 15 and 22 different bipolar cell types have been identified based on serial electron microscopy and single-cell tr...

The physiological dependence of animals on dietary intake of vitamins, amino acids, and fatty acids is ubiquitous. Sharp differences in the availability of these vital dietary biomolecules among different resources mean that consumers must adopt a range of strategies to meet their physiological needs.We review the emerging work on omega-3 long-chai...

Recent studies indicate that yawning evolved as a brain cooling mechanism. Given that larger brains have greater thermolytic needs and brain temperature is determined in part by heat production from neuronal activity, it was hypothesized that animals with larger brains and more neurons would yawn longer to produce comparable cooling effects. To tes...

A Correction to this paper has been published: 10.1038/s41593-020-00768-3.

A Correction to this paper has been published: https://doi.org/10.1038/s41593-020-00779-0.

In the vertebrate retina, amacrine and ganglion cells represent the most diverse cell classes. They can be classified into different cell types by several features, such as morphology, light responses, and gene expression profile. Although birds possess high visual acuity (similar to primates which we used here for comparison) and tetrachromatic co...

Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nL...

Embryos, juveniles, and even adults of many bird species lack pronounce external sexually dimorphic characteristics. Accurate identification of sex is crucial for research (e.g. developmental, population, and evolutionary studies), management of wildlife species, and captive breeding programs for both conservation and poultry. An accurate molecular...

Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nL...

Body growth is typically thought to be indeterminate in ectothermic vertebrates. Indeed, until recently, this growth pattern was considered to be ubiquitous in ectotherms. Our recent observations of a complete growth plate cartilage (GPC) resorption, a reliable indicator of arrested skeletal growth, in many species of lizards clearly reject the ubi...

Several groups of mammals use the Earth's magnetic field for orientation, but their magnetosensory organ remains unknown. The Ansell's mole-rat (Fukomys anselli, Bathyergidae, Rodentia) is a microphthalmic subterranean rodent with innate magnetic orientation behaviour. Previous studies on this species proposed that its magnetoreceptors are located...

Within-species variation in the number of neurons, other brain cells and their allocation to different brain parts is poorly studied. Here, we assess these numbers in a squamate reptile, the Madagascar ground gecko (Paroedura picta). We examined adults from two captive populations and three age groups within one population. Even though reptiles exh...

A community-based transcriptomics classification and nomenclature of neocortical cell types To understand the function of cortical circuits, it is necessary to catalog their cellular diversity. Past attempts to do so using anatomical, physiological or molecular features of cortical cells have not resulted in a unified taxonomy of neuronal or glial...

The stereotyped features of brain structure, such as the distribution, morphology and connectivity of neuronal cell types across brain areas, are those most likely to explain the remarkable capacity of the brain to process information and govern behaviors. Recent advances in anatomical methods, including the simple but versatile isotropic fractiona...

Squamate reptiles are considered to exhibit indeterminate growth. Nevertheless, current literature disputes the available definitions of this growth type, presents new theoretical models, and questions its universality in cold-blooded vertebrates. We have followed up on our previous research employing micro-CT to explore growth plate cartilage (GPC...

To understand the function of cortical circuits it is necessary to classify their underlying cellular diversity. Traditional attempts based on comparing anatomical or physiological features of neurons and glia, while productive, have not resulted in a unified taxonomy of neural cell types. The recent development of single-cell transcriptomics has e...

We used immunocytochemistry to determine the presence and topographical density distributions of rods, cones, and intrinsically photosensitive retinal ganglion cells (ipRGCs) in the four-striped field mouse (Rhabdomys pumilio) and the Namaqua rock mouse (Micaelamys namaquensis). Both species possessed duplex retinas that were rod dominated. In R. p...

The social brain hypothesis (SBH) posits that the demands imposed on individuals by living in cohesive social groups exert a selection pressure favouring the evolution of large brains and complex cognitive abilities. Using volumetry and the isotropic fractionator to determine the size of and numbers of neurons in specific brain regions, here we tes...

Growth plate cartilage (GPC) is responsible for the growth of long bones due to endochondral ossification, which is the main mechanism of longitudinal skeletal growth in tetrapods. Degradation of GPC is a sign of determinate growth as it arrests the growth irreversibly. By contrast, indeterminate growth requires the persistence of GPC throughout th...

To what extent can the mammalian visual system be shaped by visual behavior? Here we analyze the shape of the visual fields, the densities and distribution of cells in the retinal ganglion-cell layer and the organization of the visual projections in two species of facultative non-strictly subterranean rodents, Spalacopus cyanus and Ctenomys talarum...

Vision has long been considered purposeless in the dark underground ecotope. However, recent anatomical studies revealed an unexpected diversity of ocular and retinal features and various degrees of development of the visual system in mammals with predominantly subterranean activity, and have suggested retention of basic visual capabilities even in...

The social brain hypothesis (SBH) contends that cognitive demands associated with living in cohesive social groups favour the evolution of large brains. Although the correlation between relative brain size and sociality reported in various groups of birds and mammals provides broad empirical support for this hypothesis, it has never been tested in...

Magnetoreception has been convincingly demonstrated in only a few mammalian species. Among rodents, magnetic compass orientation has been documented in four species of subterranean mole rats and two epigeic (i.e. active above ground) species-the Siberian hamster and the C57BL/6J mouse. The mole rats use the magnetic field azimuth to determine compa...

About 300 species of mammals have adapted to the dark underground ecotope. Despite a long history of underground existence, many strictly subterranean species have retained structurally normal eyes possessing the capability of image-forming vision. Moreover, their retinae often feature high cone proportions, an indication of conserved photopic (day...

Comparative developmental studies of the mammalian brain can identify key changes that can generate the diverse structures and functions of the brain. We have studied how the neocortex of early mammals became organized into functionally distinct areas, and how the current level of cortical cellular and laminar specialization arose from the simpler...

The dorsolateral area of the hippocampal formation of birds is commonly assumed to play a central role in processing information needed for geographical positioning and homing. Previous work has interpreted odour-induced activity in this region as evidence for an 'olfactory map'. Here, we show, using c-Fos expression as a marker, that neuronal acti...

Magnetic alignment (MA) constitutes the simplest directional response to the geomagnetic field. In con- trast to magnetic compass orientation, MA is not goal directed and represents a spontaneous, fixed directional response. Because animals tend to align their bodies along or perpendicular to the magnetic field lines, MA typically leads to bimodal...

While magnetoreception in birds has been studied intensively, the literature on magnetoreception in bony fish, and particularly in non-migratory fish, is quite scarce. We examined alignment of common carps (Cyprinus carpio) at traditional Christmas sale in the Czech Republic. The sample comprised measurements of the directional bearings in 14,537 i...

Evidence for magnetoreception in mammals remains limited. Magnetic compass orientation or magnetic alignment has been conclusively demonstrated in only a handful of mammalian species. The functional properties and underlying mechanisms have been most thoroughly characterized in Ansell's mole-rat, Fukomys anselli, which is the species of choice due...

List of abbreviations. (DOCX)

The highly prevalent parasite Toxoplasma gondii reportedly manipulates rodent behavior to enhance the likelihood of transmission to its definitive cat host. The proximate mechanisms underlying this adaptive manipulation remain largely unclear, though a growing body of evidence suggests that the parasite-entrained dysregulation of dopamine metabolis...

Hert et al. (J Comp Physiol A, 2011) challenged one part of the study by Begall et al. (PNAS 105:13451–13455, 2008) claiming that they could not replicate the finding of preferential magnetic alignment of cattle recorded in aerial images of Google Earth. However, Hert and co-authors used a different statistical approach and applied the statistics o...

The African mole-rats (Bathyergidae, Rodentia) are strictly subterranean, congenitally microphthalmic rodents that are hardly ever exposed to environmental light. Because of the lack of an overt behavioural reaction to light, they have long been considered to be blind. However, recent anatomical studies have suggested retention of basic visual capa...

The neural substrate subserving magnetoreception and magnetic orientation in mammals is largely unknown. Previous experiments have demonstrated that the processing of magnetic sensory information takes place in the superior colliculus. Here, the effects of magnetic field conditions on neuronal activity in the rodent navigation circuit were assessed...

Resting and grazing cattle and deer tend to align their body axes in the geomagnetic North-South direction. The mechanism(s) that underlie this behavior remain unknown. Here, we show that extremely low-frequency magnetic fields (ELFMFs) generated by high-voltage power lines disrupt alignment of the bodies of these animals with the geomagnetic field...

We have studied the visual system of subterranean mole-rats of the rodent family Bathyergidae, for which light and vision seem of little importance. The eye diameter varies between 3.5mm in Bathyergus suillus and 1.3mm in Heterocephalus glaber. The small superficial eyes have features typical of sighted animals (clear optics, well-developed pupil a...

Within the family Bathyergidae (subterranean African mole-rats), Cryptomys and Coetomys are the most speciose genera. Whereas each species can be characterized karyologically and from mitochondrial gene sequence data genetically, most of them exhibit marked intraspecific polymorphism and an overlap in morphological and biometric traits making them...

The visual system of subterranean mammals is assumed to be regressed and rudimentary in response to their lightless ecotope. Among rodents this has been exemplified by detailed studies of the blind mole-rat Spalax ehrenbergi (see Nevo 1999, this volume). However, recent studies involving a larger spectrum of subterranean mammalian species have 'une...

Living aboveground offers animals diverse cues for spatial orientation, but the underground picture is less colourful: besides darkness, subterranean rodents face restriction in useful orientation cues such as odors or sounds (Burda et al. 1990a). Target and landmarks are not directly perceivable and, as a consequence, distant orientation is heavil...

After more than two decades of intensive research, the physiological mechanisms of animal magnetoreception remain enigmatic. The primary magnetoreceptors are still unknown and our knowledge of the neural substrate subserving magnetic orientation is meagre. Here we argue that this dismal outcome can be largely attributed to the fact that the potenti...

Abstract We studied the retinal projections, the distribution of cytochrome oxidase activity and the cyto- and myeloarchitecture of the subcortical visual system in the subterranean Ansell's mole-rat Cryptomys anselli. The optic nerve contained 1500 myelinated and a similar number of unmyelinated fibres. The retina projected to all the visual struc...

We have determined the presence of spectral cone types, and the population densities of cones and rods, in subterranean mole-rats of the rodent family Bathyergidae, for which light and vision seems of little importance. Most mammals have two spectral cone types, a majority of middle- to long-wave-sensitive (L-) cones, and a minority of short-wave-s...

The neural substrate subserving magnetic orientation is largely unknown in vertebrates and unstudied in mammals. We combined a behavioral test for magnetic compass orientation in mole rats and immunocytochemical visualization of the transcription factor c-Fos as a marker of neuronal activity. We found that the superior colliculus of the Zambian mol...