Pavel Cejnar

• Doctor of Engineering
• Research Assistant at University of Chemistry and Technology, Prague

Accumulation of extracellular matrix (ECM) in liver fibrosis is associated with changes in protein abundance and composition depending upon etiology of the underlying liver disease. Current efforts to unravel etiology-specific mechanisms and pharmacological targets rely on several models of experimental fibrosis. Here, we characterize and compare d...

Accumulation of extracellular matrix (ECM) in liver fibrosis is associated with changes in protein abundance and composition depending upon etiology of the underlying liver disease. Current efforts to unravel etiology- specific mechanisms and pharmacological targets rely on several models of experimental fibrosis. Here, we characterize and compare...

Accumulation of extracellular matrix (ECM) in liver fibrosis is associated with changes in protein abundance and composition depending upon etiology of the underlying liver disease. Current efforts to unravel etiology- specific mechanisms and pharmacological targets rely on several models of experimental fibrosis. Here, we characterize and compare...

Accumulation of extracellular matrix (ECM) in liver fibrosis is associated with changes in protein abundance and composition depending upon etiology of the underlying liver disease. Current efforts to unravel etiology-specific mechanisms and pharmacological targets rely on several models of experimental fibrosis. Here, we characterize and compare d...

Vestibular schwannoma is the most common benign neoplasm of the cerebellopontine angle. Its first symptoms include hearing loss, tinnitus, and vestibular symptoms, followed by cerebellar and brainstem symptoms, along with palsy of the adjacent cranial nerves. However, the clinical picture has unpredictable dynamics and currently, there are no relia...

Cannabidiol (CBD) is the non-psychoactive component of the plant Cannabis sativa (L.) that has great anti-inflammatory benefits and wound healing effects. However, its high lipophilicity, chemical instability, and extensive metabolism impair its bioavailability and clinical use. Here, we report on the preparation of a human cornea substitute in vit...

Bean common mosaic virus from the genus Potyvirus has a wide range of hosts and a very negative impact on cultivated crops from the genus Phaseolus. The risk of viral infection of economically important crops increases even if the carriers of the virus are related plant species growing on agroecological interfaces. Such plant species have emerged a...

Bean common mosaic virus from the genus Potyvirus has a wide range of hosts and a very negative impact on cultivated crops from the genus Phaseolus. The risk of viral infection of economically important crops increases even if the carriers of the virus are related plant species growing on agroecological interfaces. Such plant species have emerged a...

The aim of this work is to offer an alternative or complementary analytical tool to the time‐consuming and expensive methods commonly used for the recognition of animal species according to their hair. The paper introduces a simple and fast way for species differentiation of animal hairs called in‐sample digestion. A total of 10 European animal spe...

Twenty-four blood serum samples from patients with acute methanol poisoning (M) from the mass methanol poisoning outbreak in the Czech Republic in 2012 were compared with 46 patient samples taken four years after poisoning (S) (overlap of 10 people with group M) and with a control group (C) of 24 samples of patients with a similar proportion of chr...

For the understanding of pathological states of bone tissues in oral surgery, it would be desirable to have a possibility to simulate these processes on bone cell models in vitro. These cultures, similarly like bone tissues, contain numerous proteins entrapped in the insoluble matrix. The major goal of this study was to verify whether method based...

Detection of adulteration of small ruminant milk is very important for health and commercial reasons. New analytical and cost-effective methods need to be developed to detect new adulteration practices. In this work, we aimed to explore the ability of the MALDI-TOF mass spectrometry to detect bovine milk in caprine and ovine milk using samples from...

Anthropometric studies focusing on facial metrics and their proportions form an important research area devoted to observations of the appearance of the human skull. Many different applications include the use of craniometry for maxillofacial reconstruction and surgery. This paper explores the possibility of using selected craniometric points and a...

A tomato plant (Solanum lycopersicum Linnaeus, labelled KVE) displaying virus-like symptoms, tested negative for common tomato viruses, was subjected to high-throughput sequencing (HTS) on the Illumina MiSeq platform using ribosomal RNA-depleted total RNA as a template. The analysis has revealed the contigs mapping to Lettuce big-vein associated vi...

At present, Alzheimer´s disease is detected mainly using psychological tests, which, can only confirm the disease in its more advanced phases. Therefore, bioanalytical possibilities for detecting this disease earlier are being investigated. To date, the results of analyses, which focus mainly on the study of lipids and proteins either in cerebrospi...

Proteomic characterization of alveolar bones in oral surgery represents an analytical challenge due to their insoluble character. The implementation of a straightforward technique could lead to the routine use of proteomics in this field. We have thus developed a simple technique for the characterization of bone tissue for human maxillary and mandi...

Gait disorders accompany a number of neurological and musculoskeletal disorders that significantly reduce the quality of life. Motion sensors enable high-quality modelling of gait stereotypes. However, they produce large volumes of data, the evaluation of which is a challenge. In this publication, we compare different data reduction methods and cla...

Enrichment of mortar mixtures by the addition of natural organic materials has a long history. Already in ancient times it has been empirically proved that proteinaceous additives significantly increase mortar durability, strength or plasticity. The knowledge of the protein occurrence in historical mortars would be very useful for the restoration w...

The recognition of motion patterns belongs to very important research areas related to neurology, rehabilitation, and robotics. It is based on modern sensor technologies and general mathematical methods, multidimensional signal processing, and machine learning. The present paper is devoted to the detection of features associated with accelerometric...

Ataxic gait monitoring and assessment of neurological disorders belong to important multidisciplinary areas that are supported by digital signal processing methods and machine learning tools. This paper presents the possibility of using accelerometric data to optimise deep learning convolutional neural network systems to distinguish between ataxic...

Texture analysis and classification of image components belong to common problems of the interdisciplinary area of digital signal and image processing. The paper is devoted to the pattern matrix construction using features evaluated by the discrete Fourier transform (DFT) or the discrete wavelet transform (DWT) using the relative power in selected...

Plant viruses are important pathogens that cause significant crop losses. A plant protein extraction protocol that combines crushing the tissue by a pestle in liquid nitrogen with subsequent crushing by a roller-ball crusher in urea solution, followed by RuBisCO depletion, reduction, alkylation, protein digestion, and ZipTip purification allowed us...

The commonly used histological assessment of pathological states of alveolar bone tissues in oral surgery needs laborious and time‐consuming processing by an experienced histologist. Therefore, a simpler and faster methodology is required in this field. Following this demand, this paper reports a straightforward approach using the tryptic cleavage...

While methods like radiology or histology are routinely used in oral surgery, a simple and effective method for characterization of alveolar bones at the molecular level is missing. This is primarily due to the fact that proteomic analysis of hard tissue represents a serious analytical challenge. In this study, we developed a simple technique for t...

Due to known information processing capabilities of the brain, neurons are modeled at many different levels. Circuit theory is also often used to describe the function of neurons, especially in complex multi-compartment models, but when used for simple models, there is no subsequent biological justification of used parts. We propose a new single-co...

Identification of proteinaceous materials in artworks is of high interest to restorers-conservators, art historians and archaeologists, because it helps to shed light on the used painting techniques, to attribute unknown artworks, to make conclusions on prehistoric diets, etc. The chapter is devoted to the mass spectrometry instrumentation, evaluat...

Background Although many genetic manipulations of crops providing biofortified or safer food have been done, the acceptance of biotechnology crops still remains limited. We report on a transgenic barley expressing the multi-functional protein osmotin that improves plant defense under stress conditions. Methods An Agrobacterium–mediated technique w...

Scheme of the expression vector pBRACT214 containing the osmotin gene. (TIF)

Regeneration of transgenic barley var. Golden Promise after transformation with osmotin gene. a) Regenerating plantlets from calluses after 6 wk on selection medium. b) Putative transgenic plants on regenerating medium. c) Transgenic plants T0 generation in greenhouse. (TIF)

Detection of osmotin transgene in the genomic DNA of T0 regenerants. Lane 1–11: samples; lane 12: negative control (DNA/RNA free water); lane 13: positive control (plasmid pBRACT214::osm); lane 14: negative control (genomic DNA of WT plants); lane 15: DNA standard (50 bp DNA ladder, Bioline). Size of PCR product is 222 bp. (TIF)

Detection of transgene encoding the marker for selection (hpt gene) in the genomic DNA of T0 regenerants. Lane 1–3: samples; lane 4: negative control (DNA/RNA free water); lane 5: negative control (genomic DNA of WT plants); lane 6: positive control (hpt positive plant), lane 7: DNA standard (2-kb DNA ladder, Bioline). Size of PCR product is 275 bp...

Exposition of transgenic and control barley to fungi infection. left: Non-transgenic barley (left) versus transgenic barley bearing tobacco osmotin gene (right) after biotic stress (15 days after first spraying of Fusarium oxysporum spores). Right: symptoms recognized on non-transgenic barley leaves after stress (chlorosis, necrosis, premature leaf...

Although many genetic manipulations of crops providing biofortified or safer food have been prepared, the acceptance of biotechnology crops still remains limited. We report on a transgenic barley expressing the multi-functional protein osmotin that improves plant defense under stress conditions. An Agrobacterium–mediated technique was used to trans...

Identification of proteinaceous materials in artworks is of high interest to restorers-conservators, art historians and archaeologists, because it helps to shed light on the used painting techniques, to attribute unknown artworks, to make conclusions on prehistoric diets, etc. The chapter is devoted to the mass spectrometry instrumentation, evaluat...

We constructed Wheat dwarf virus (WDV) infectious clones in the bacterial plasmids pUC18 and pIPKb002 and tested their ability to inoculate plants using Bio-Rad Helios Gene Gun biolistic inoculation method and Agrobacterium tumefaciens agroinoculation method, and we then compared them with the natural inoculation method via viruliferous P. alienus....

The brain is a complex organ responsible for memory storage and reasoning; however, the mechanisms underlying these processes remain unknown. The paper forms a contribution to a lot of theoretical studies devoted to regular or chaotic oscillations of interconnected neurons assuming that the smallest information unit in the brain is not a neuron but...

Wheat dwarf virus (WDV), an important cereal pathogen, is closely related to Maize streak virus (MSV), a model virus of the Mastrevirus genus. Based on its similarity to known MSV resistance strategies, a truncated part of the WDV replication-associated (RepA) gene (WDVRepA215) and the WDV RepA gene with a mutated retinoblastoma-related protein (RB...

In this dataset we provide MALDI-TOF MS spectra of Escherichia coli. The data presented in this article are related to the article entitled “Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from MALDI-TOF” (Božik et al., 2018) [1]. Essential oils and their components are known for their ant...

The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on lo...

Table S1: Minimum inhibitory concentrations of tested compounds against E. coli by the microdilution method ---- Figure S2: Principal component analysis of the average spectra for each time and treatment – samples scores plot for PC1×PC2 projection (top) and PC1×PC3 projection (bottom). Samples from 0, 90 and 120 minutes were included.

Normalized relative peak intensity measured by MALDI-TOF-MS in Escherichia coli treated by essential oils components, peroxide, chlorine and tetracycline, 90 after treatment.

Identified proteins and their functions

Multimodal signal analysis based on sophisticated sensors, efficient communication systems and fast parallel processing methods has a rapidly increasing range of multidisciplinary applications. The present paper is devoted to pattern recognition, machine learning, and the analysis of sleep stages in the detection of sleep disorders using polysomnog...

Rationale: Explorative statistical analysis of mass spectrometry data is still a time-consuming step. We analyzed critical factors for application of principal component analysis in mass spectrometry and focused on two whole spectrum based normalization techniques and their application in analysis of registered peak data and, in comparison, in ful...

Crustaceans are vital components of the freshwater zooplankton that link various trophic levels. Because crustaceans, particularly cladocerans of the Daphnia genus, are sensitive to environmental changes, they can serve as indicators of environmental fluctuations. Therefore, it is highly desirable to have a fast and reliable method for the identifi...

The paper presents specific methods of processing of multimodal data recorded during physical activities by depth MS Kinect cameras, thermal imaging cameras and heart rate sensors. All video data and heart rate signals used in the present study were recorded in the home environment. The proposed methodology includes the detection of the chest breat...

Matrix‐assisted laser desorption/ionisation–time of flight (MALDI‐TOF) mass spectrometry is commonly used for the identification of proteinaceous binders and their mixtures in artworks. The determination of protein binders is based on a comparison between the m/z values of tryptic peptides in the unknown sample and a reference one (egg, casein, ani...

Non-contact methods for the tracking of breathing have found noticeable interest in research in recent years motivated by the obtrusiveness of the traditional approach to sleep disordered breathing diagnosis. The low-priced Kinect device released by Microsoft has emerged as a possible alternative hardware in the field of subject's monitoring aimed...

The paper presents in its initial part fundamental mathematical tools for analysis of retinal images using methods of digital signal processing and enhancement and methods for their registration to monitor changes during the treatment. Blood vessel trees are then extracted to analyse their specific structures to find retina disorders. The proposed...

Artificial Intelligence is a highly creative field. Numerous research areas in Computer Science that originated over the past fifty years within AI laboratories and were discussed in AI conferences are now completely independent and mature research ...

The computer constructed local search heuristic function for a SAT problem can run and solve SAT faster than human designed heuristics. The idea behind this was to start with some predefined primitives chosen in accordance with a human observation and to combine them using genetic programming. This leads to a question whether it is possible to cons...