Paulo Mascarenhas

Egaz Moniz School of Health and Science · Instituto Superior de Ciências da Saúde Egas Moniz

This systematic review assessed the prevalence of transmitted and acquired HIV drug resistance (HIVDR) and the associated risk factors in Mozambique. A search of the PubMed, Cochrane, B-On, and Scopus databases up to December 2023 was conducted and included 11 studies with 1118 HIV-1 pol sequences. Drug resistance mutations (DRMs) to NNRTIs were fo...

Objectives This study aims to evaluate the cytotoxicity and genotoxicity of three different extracts obtained from Filtek™ One Bulk Fill, Tetric Evoceram® Bulk Fill and Coltene Fill-Up! resins. Materials and methods The cytotoxicity was determined on 3T3 fibroblast cells using the MTT and crystal violet assays. The genotoxicity was determined usin...

This study was conducted to assess the oral status of nursing home residents diagnosed with dementia in the Lisbon region, Portugal. In this cross-sectional observational pilot study, the oral and dental status were evaluated by determining the Decayed-Missing-Filled-Tooth (DMFT) index, frequency of oral hygiene, presence of erosion lesions or muco...

Aging can have a negative impact on the perioral region, affecting both appearance and self-esteem. Dentists offer various treatment options to address these changes, using surgical and non-surgical techniques. One such technique is facial filling with hyaluronic acid, a minimally invasive approach to improve the perioral region’s function and aest...

Dementia is a rising public health concern. Feeding and nutritional problems increase as the disease progresses, affecting the clinical course and caregiver burden. While some guidelines advise against percutaneous endoscopic gastrostomy (PEG) and tube feeding in advanced dementia, conflicting evidence exists. This study aims to evaluate the nutrit...

Dementia is a rising public health concern. Feeding and nutritional problems increase as the disease progresses, affecting clinical course and caregiver burden. While some guidelines advise against percutaneous endoscopic gastrostomy (PEG) and tube feeding in advanced dementia, conflicting evidence exists. This study aims evaluating nutritional sta...

For percutaneous endoscopic gastrostomy (PEG)-fed head and neck cancer (HNC) patients, risk markers of poor outcomes may identify those needing more intensive support. This retrospective study aimed to evaluate markers of poor outcomes using TNM-defined stages, initial anthropometry [body mass index (BMI), mid-upper arm circumference (MUAC), tricip...

Pediatric overweight, dyslipidemia and insulin resistance can result from unhealthy lifestyle habits and increase morbidity and mortality in adulthood. Herein, we evaluated the relationship between diet and physical activity patterns with the metabolic health of 9-year-old school children. Measurements included anthropometry, adiposity, lipid, and...

Oral diseases are highly prevalent worldwide. Recent studies have been supporting a potential bidirectional association of oral diseases with systemic noncommunicable diseases (NCDs). Available evidence supports that people with NCDs have a greater prevalence of oral diseases particularly those with limited ability of oral self-care. Regarding the...

Scientific evidence regarding the incidence of dental caries in Down syndrome (DS) patients is limited and sometimes presents divergent opinions among authors, making it difficult to reach definitive conclusions. We aimed to evaluate the caries incidence in the DS pediatric population and compare it against healthy controls. The search was performe...

When implants are required in prosthodontics treatment, one of the most important decisions is the choice of the final crown and the type of connection to the implant through the abutment. Hybrid abutments are becoming a primary choice. They are projected and produced with materials whose properties guarantee the required mechanical features (inclu...

Feature Application: The evaluation of genetic polymorphisms may have great clinical relevance since they can be measured before the onset of the disease and may be of great benefit for treatment planning and prognosis at an early stage. Abstract: Recent studies report that individuals with polymorphisms in the genes that encode for interleukin (IL...

Most hospitalized COVID-19 pneumonia patients are older adults and/or have nutrition-related issues. Many are bedridden in intensive care units (ICU), a well-documented cause of malnutrition, muscle wasting, and functional impairment. Objectives: To assess the effectiveness of an intensive rehabilitation program over the nutritional/functional stat...

Studies heretofore have shown inconsistent results on the link of ASD to malocclusion. Herein, we aimed to compare the prevalence of malocclusion among children and adolescents with ASD compared with non-ASD healthy counterparts through a systematic review. The electronic search focused on five databases, PubMed, Web of Science, EMBASE, LILACS, and...

Background: The aim of this systematic review and network meta-analysis was to assess the efficacy of a bilaminar root coverage technique consisting of the combination of an autogenous subepithelial connective tissue graft (SCTG) and a coronally advanced flap (CAF) compared to the five most indicated alternative approaches for the treatment of sing...

Oral conditions are highly prevalent worldwide. Recent studies have been supporting a potential bidirectional association of oral disorders with systemic noncommunicable diseases (NCDs). Robust evidence supports the greater prevalence of oral conditions in people suffering from NCDs limiting the ability of oral self-care. As for the relationship wi...

Background: Endoscopic Gastrostomy (PEG) is required to maintain a feeding route when neurological or cancer dysphagia impact oral intake. This study aimed to evaluate the oral health and oral changes of PEG-patients without oral feeding for three months. Methods: Prospective observational study, with a PEG-patients convenience sample. Data were...

Background: The timing of growth is a key factor for correct orthodontic treatment planning. Cervical vertebrae maturation (CVM) is no exception, although the reported chronological ages vary in the literature. Objective: We aimed to estimate the average chronological age for each Baccetti's CVM staging. Search methods: Search on MEDLINE-PubMe...

In postpubertal patients, maxillary transverse discrepancy is a common condition often requiring surgical approaches. To overcome the excess morbidity and discomfort, maxillary expansion through miniscrew-assisted rapid palatal expansion (MARPE) was proposed and studied in the last few years. This umbrella review aims to critically appraise the qua...

Nutrition is an important health issue for seniors. In nursing homes, simple, inexpensive, fast, and validated tools to assess nutritional risk/status are indispensable. A multisurvey cross-sectional study with a convenient sample was created, comparing five nutritional screening/assessment tools and the time required for each, in order to identify...

Molar-Incisor Hypomineralization (MIH) is a qualitative defect of enamel of unknown etiology, affecting one or more permanent molars and may include incisors. This condition is a clinical challenge and its prevalence is still uncertain given the recent increase in research. Thus, we aimed to comprehensively estimate the overall prevalence of MIH an...

Periodontitis has been associated with low-grade inflammation as assessed by C-reactive protein (CRP) levels and its treatment can decrease CRP serum levels. The aim of this systematic review was to critically appraise the evidence comparing CRP serum levels (standard and high-sensitivity [hs]) of otherwise healthy patients suffering from periodont...

This systematic review and meta-analysis aimed to assess the literature about the incidence of oral mucositis and its degrees (mild, moderate, and severe), in patients undergoing head and neck cancer treatment (radiotherapy, chemotherapy, and surgery). Addressing this issue is important since oral mucositis has a negative impact on oral health and...

The goal of cancer treatment is to fight and/or control cancer. The aim of this study was to review and meta-analyse the incidence of main oral complications in paediatric oncology during chemotherapy. The search results were obtained from B-on, Web of Science, Scopus, Cochrane Library and PubMed databases. Of 1032 articles potentially relevant, 13...

The interindividual variability of Proton Pump Inhibitor (PPI) therapy results from the phenotype variability associated with the cytochrome P450 2C19 (CYP2C19) gene, namely the CYP2C19*17 allele. Our aim was to characterize patients’ genetic variability undergoing PPI therapy. A sample of 33 oral mucosa cells from Portuguese pharmacy patients was...

In vitro studies evaluating the cytotoxic potential of substances released from dental adhesives are lacking. The purpose of this study was to compare the cytotoxicity of the extracts of dental adhesives Scotchbond Universal and Optibond Solo Plus, and an adhesive in the experimental phase: T1. 3T3 mouse fibroblast cells and MG-63 osteoblast-like c...

The clinical interaction between stroke and periodontitis has been consistently studied and confirmed. Hence, exploring potentially new protein interactions in this association using bioinformatic strategies presents potential interest. In this exploratory study, we conducted a protein– protein network interaction (PPI) search with documented encod...

The latest evidence revealed a possible association between periodontitis and Parkinson’s disease (PD). We explored the causal relationship of this bidirectional association through two-sample Mendelian randomization (MR) in European ancestry populations. To this end, we used openly accessible data of genome-wide association studies (GWAS) on perio...

Aim To evaluate the risk factors / predictors of tooth loss in patients with periodontitis who underwent periodontal therapy and long‐term periodontal maintenance (PM). Material & Methods PUBMED, CENTRAL, EMBASE, Web of Science, LILACS and Scholar were searched up to and including September 2020. Studies limited to periodontitis patients who under...

Introduction Thermoresponsive polymers have been widely investigated in modern drug delivery and gene delivery systems, due to their potential to load and release the payload by simply modifying local temperature [1]. Previous work by some of us has shown that the thermoresponsive copolymers poly(N-isopropylacrylamide)n-block-poly((3-acrylamidoprop...

Introduction Bleaching teeth to have a whiter and bright smile is a popular trend and currently one can do it at home, without technical support from dentists. However, whitening products are not innocuous and the European legislation is clear limiting the content in hydrogen peroxide (H2O2), present or released, to 6%, still, products containing u...

Citation: Moreira, V.M.; Mascarenhas, P.; Machado, V.; Botelho, J.; Mendes, J.J.; Taveira, N.; Almeida, M.G. Diagnosis of SARS-Cov-2 Infection by RT-PCR Using Specimens Other Than Naso-and Oropharyngeal Swabs: A Systematic Review and Meta-Analysis. Diagnostics 2021, 11, 363. https://doi.

The clinical interaction between stroke and periodontitis has been consistently studied and confirmed. Hence, forecasting potentially new protein interactions in this association using bioinformatic strategies presents potential interest. In this exploratory study, we conducted a protein-protein network interaction (PPI) search with documented enco...

Statement of problem Dental cements that release monomers that negatively impact adjacent oral soft tissues may adversely affect clinical outcomes. However, in vitro studies evaluating the cytotoxic and genotoxic potential of substances released from dental cements are lacking. Purpose The purpose of this in vitro study was to define and compare t...

Background The rapid and accurate testing of SARS-CoV-2 infection is still crucial to mitigate, and eventually halt, the spread of this disease. Currently, nasopharyngeal swab (NPS) and oropharyngeal swab (OPS) are the recommended standard sampling, yet, with some limitations. Several specimens that are easier to collect are being tested as alterna...

Recent studies supported a clinical association between Parkinson’s disease (PD) and periodontitis. Hence, investigating possible interactions between proteins associated to these two conditions is of interest. In this study, we conducted a protein–protein network interaction analysis with recognized genes encoding proteins with variants strongly a...

This systematic review investigates the failure rate and marginal bone loss (MBL) of dental implants placed in patients undergoing solid-organ transplant (SOT) compared to healthy controls. Three databases (PubMed, Web of Sciences, and the Cochrane Library) were searched up to June 2020 (PROSPERO CRD42019124896). Case-control and cohort studies rep...

Latest evidence revealed a possible association between Parkinson’s disease (PD) and periodontitis. We explored the causal relationship of this association through two-sample Mendelian randomization (MR) in European ancestry populations. To this end, we used openly accessible data of genome-wide association studies (GWAS) on PD and periodontitis. A...

Recent studies supported a clinical association between Parkinson’s Disease (PD) and periodontitis. Hence, investigating possible protein interactions between these two conditions is of interest. In this study, we conducted a protein-protein network interaction analysis with recognized genes encoding proteins for PD and periodontitis. Genes of inte...

This systematic review investigates the failure rate and marginal bone loss (MBL) of dental implants placed in Solid-organ transplant (SOT) patients compared to healthy controls. Three databases (PubMed, Web of Sciences and the Cochrane Library) were searched up to June 2020 (PROSPERO CRD42019124896). Case-control and cohort studies reporting data...

Objectives This study aims to evaluate the cytocompatibility of three provisional restoration materials and predict neurotoxic potential of their monomers. These materials are Tab 2000® (methyl methacrylate based), ProTemp 4™ (bis-acrylic based) and Structur 3® (urethane dimethacrylate based). Materials and methods Resin samples were incubated in...

Introduction The purpose of this study was to seek and summarise the Bolton overall index (OI) and anterior index (AI) regarding normal occlusion and Angle’s malocclusion according to gender, and to assess if these indices support Bolton’s standards as general references. Methods PubMed, LILACS, Embase, CENTRAL and Google Scholar databases were se...

Introduction: The purposes of this study were to seek and summarize overall index (OI) and anterior index (AI) regarding normal occlusion and Angle’s malocclusion and according to gender, to assess if such results support Bolton’s standards as general references. Methods: Pubmed, LILACS, Embase, CENTRAL and Google Scholar databases were search...

This retrospective study aimed to investigate the effect of known risk factors on nonsurgical periodontal treatment (NSPT) response using a pocket depth fine-tuning multilevel linear model (MLM). Thirty-seven patients (24 males and 13 females) with moderate to severe chronic periodontitis were treated with nonsurgical periodontal therapy. Follow-up...

Introduction: Periodontitis presents complex bacterial biofilms and multifactorial mechanisms, being its management a major challenge [1–3 Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol Nature Publishing Group. 2010;8(7):481–490. doi:10.1038/nrmicro2337 Arweiler NB, Auschill TM, Sculean A. Patient self-...

Introduction: Polymethylmethacrylate resins are essential materials in the Oral Rehabilitation field. However, their use is being called into question once they can cause adverse reactions in the adjacent tissues [1 Goiato MC, Freitas E, Dos Santos D, et al. Acrylic resin cytotoxicity for denture base – Literature review. Adv Clin Exp Med. 2015;24:...

Introduction: Periodontitis presents complex bacterial biofilms and multifactorial mechanisms, being its management a major challenge [1–3 Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol Nature Publishing Group. 2010;8(7):481–490. Arweiler NB, Auschill TM, Sculean A. Patient self-care of periodontal pock...

Introduction: The cardiovascular effects of the exposure to low frequency noise (LFN) (<500 Hz) are not fully understood. Previous experimental work confirmed an increase in collagens I and III and ultrastructural changes in the intercalated discs of cardiomyocytes from rats exposed to LFN. Our work aims at characterizing the alterations in gene ex...

Introduction: Dolutegravir is an HIV-1 integrase inhibitor (INI) recently approved for the treatment of HIV infection and is metabolized by UGT1A1 [1 Yagura H, Watanabe D, Kushida H, et al. Impact of UGT1A1 gene polymorphisms on plasma dolutegravir trough concentrations and neuropsychiatric adverse events in Japanese individuals infected with HIV-1...

Introduction: Besides the knowledge of life cycles in nature, the sustained use of aquatic biological resources also requires the development of know-how to reproduce and maintain them in captivity [1 Goldstein J, Steiner UK. Ecological and demographic drivers of jellyfish blooms. bioRxiv. 2017;102814. [Google Scholar]]. The scyphozoan Catostylus t...

Introduction: Different cancer cells, including those from breast cancer, show increased APOBEC3 activity [1 Alexandrov LB, Nik-Zainal S, Wedge DC, et al. Signatures of mutational processes in human cancer. Nature. 2013;500:415–421.[Crossref], [PubMed], [Web of Science ®] , [Google Scholar],2 Roberts SA, Lawrence MS, Klimczak LJ, et al. An APOBEC c...

Introduction: Virion Infectivity factor (Vif) is a protein required for lentiviruses successful infection of human target cells, through the degradation of host restriction factors, APOBEC3 [1 Goila-Gaur R, Strebel K. HIV-1 Vif, APOBEC, and intrinsic immunity. Retrovirology. 2008;5:1–16.[Crossref], [PubMed], [Web of Science ®] , [Google Scholar]]....

Introduction: The purposes of this study were to seek for overall ratio (OR) and anterior ratio (AR) patients data in normal occlusion and Angle’s malocclusion studies, and to assess if such results support Bolton’s standards as general references. Methods: Pubmed, Medline, CENTRAL and Scholar databases were searched up to February 2018 (CRD4201808...

Introduction: The purposes of this study were to seek for overall ratio (OR) and anterior ratio (AR) patients data in normal occlusion and Angle’s malocclusion studies, and to assess if such results support Bolton’s standards as general references. Methods: Pubmed, Medline, CENTRAL and Scholar databases were searched up to February 2018 (CRD4201808...

Objectives: This study aimed to assess bias magnitudes, sensibility, and specificity of particular partial-mouth recording protocols (PRPs) to estimate periodontal clinical measurements and periodontal status. Methods: Estimates of mean clinical attachment loss (MCAL) and mean probing depth (MPD) were calculated for 15 different PRPs and were co...

Objective: This meta-analysis aims to systematically assess whether periodontitis has a meaningful effect on salivary cortisol, reflecting changes on free blood cortisol levels. Design: The Cochrane Handbook and the PRISMA statement were used as reporting guidelines. The MEDLINE-PubMed, Google Scholar, EMBASE, and CENTRAL databases were searched un...

Purpose: Evaluate the relationship of leptin receptor (LEPR) rs1137101, fat mass obesity-associated (FTO) receptors 9939609, melanocortin-4 receptors (MC4R) rs2229616 and rs17782313, and proliferator-activated receptor-gamma (PPARG) rs1801282 with clinical and metabolic phenotypes in prepubertal children. Research question: What is the effect of...

Background Autopsy studies reveal that atherosclerosis lesions can be found as early as two years of age. To slow the development of this early pathology, obesity and dyslipidemia prevention should start from childhood making it urgent to explore new ways to evaluate dyslipidemia risk in children that can be applied widely, such as the non-invasive...

Descriptive clinical characteristics of the study population by gender and by IOTF category Δ. ΔAccording to World Obesity/Policy & Prevention and bioelectrical impedance analysis cut-offs. §Age in days were converted in years for a better comparison between groups. Sex means between each characteristic were compared within each IOTF category while...

Descriptive characteristics of the study population of children by ethnicity. §Age in days were converted in years for a better comparison between groups. 1 (Cauc-471/Afr-45/Oth-2). 2 (Cauc-458/Afr-50/Oth-8). 3 (Cauc-245/Afr-16/Oth-1). Ethnic group means are compared between each characteristic through Bonferroni-adjusted t-tests. Statistical diffe...

TRIPOD checklist—prediction model development and validation. *Items relevant only to the development of a prediction model are denoted by D, items relating solely to a validation of a prediction model are denoted by V, and items relating to both are denoted D;V. We recommend using the TRIPOD Checklist in conjunction with the TRIPOD Explanation and...

Adjusted correlations between biochemical parameters. APO A1 (apolipoprotein A1): APO B (apolipoprotein B): HDL-c (high-density lipoproteins cholesterol): LDL-c (low-density lipoproteins cholesterol): HOMA-IR (homeostatic model assessment-insulin resistance): N_HDL-c (non-HDL cholesterol): oxLDL (oxidized low-density lipoprotein): TC (total cholest...

Independent contributions of MUAC and CC to prediction of cardiovascular disease risk factors (adjusted for ethnicity, age, gender and waist circumference). * Not significant. APO A1 (apolipoprotein A1): APO B (apolipoprotein B): CC (calf circumference): HDL-c (high-density lipoproteins cholesterol): LDL-c (low-density lipoproteins cholesterol): MU...

Members of human APOBEC3 (A3) protein family are potent cellular restriction factors against HIV-1viral infection. In scientific community it is consensual that A3G is a potent inhibitor of Vif defective HIV-1 as well as A3D, A3F and A3HhapII [1]. There are few works using HIV-2 and the restriction ability of A3 members was not experimentally confi...