Paula S. Coelho

Instituto Nacional de Investigação Agrária e Veterinária (INIAV) | INRB · SAFSV

Wild rocket downy mildew (DM), caused by oomycete Hyaloperonospora sp., is a widely spread disease reducing crop production and quality. New productive wild rocket varieties resistant to DM are crucial to control disease and ensure high quality leaves. A histological characterization of Hyaloperonospora sp. infection was performed in 11 wild rocket...

4 8 HORTOFRUTICULTURA COLEÇÃO DE COUVES PORTUGUESAS, HERANÇA E FUTURO "Abrir a arca do tesouro da coleção 'Couves Portuguesas' é promover esses valiosos recursos endógenos, uma herança compartilhada pela Ibéria e outros continentes." [The promotion of the 'Portuguese Cabbages' collection represents a significant opportunity to advance the visibilit...

Introdução O míldio é uma doença causada pelo oomiceta Hyaloperonospora sp., que afeta a produção e a qualidade das folhas da rúcula selvagem (Diplotaxis tenuifolia (L.) DC). Neste estudo caracterizou-se a infeção de míldio ao nível histológico em plantas de rúcula com diferentes respostas ao míldio. É crucial a obtenção de novas variedades resiste...

Rocket is the common designation for two baby-leaf salad crops of the Brassicaceae family: Eruca sativa (L.) Cav., usually referred to as annual garden rocket, and Diplotaxis tenuifolia (L.) DC. commonly named to as perennial wild rocket. E. sativa is used for human consumption since antiquity. However, the growing consumer preference for D. tenuif...

Five-hundred ~ 500 nucleotides long SNP loci of Diplotaxis tenuifolia (L.) DC.

One hundred accessions of a “core collection” of Diplotaxis tenuifolia (L.) DC. and Eruca spp. were screened at seedling stage for resistance to downy mildew. Accessions tested at the seedling stage were assigned to 0–6 interaction phenotypes. All cultivated rocket (Eruca spp.) accessions exhibited a resistant (R) response both in cotyledons and in...

O Mediterrâneo é considerado um "hotspot" global de biodiversidade, daí a manutenção dos serviços ecossistémicos dessa paisagem ser essencial. A biodiversidade vegetal está a ser ameaçada pelas alterações climáticas e pelas atividades humanas com efeitos: (1) dinâmica e área de distribuição da população; (2) estrutura dos ecossistemas; (3) extinção...

Wild rocket is a popular baby leaf-type crop in the Brassicaceae family, associated with an healthy Mediterranean diet style, grown in Southern Europe, the Middle East and in South-East Asia. However, the high susceptibility of wild rocket to the severe foliar downy mildew (DM) disease caused by the oomycete Hyaloperonospora sp. represents an incre...

INTRODUÇÃO Em 2016, o setor hortícola na UE-27 representou 13,7% da produção agrícola total e ocupou uma pequena percen-tagem da área agrícola de cerca de 3% (EUROSTAT). Este setor caracteriza-se por uma elevada empregabilidade e acessibilidade (empregos de fácil acesso), o que é reconhecido no Relatório de Horticultura do Parlamento Europeu (UE, 2...

Radish downy mildew (DM) caused by the oomycete Hyaloperonospora brassicae f. sp. raphani is a serious problem in radish crop, an edible root vegetable of the Brassicaceae family. The objective of this research was to assess radish germplasm for DM resistance and to evaluate the response of different radish organs to the disease under controlled co...

Cultivated rocket salad (Eruca vesicaria Mill.) is a fast-growing annual herbaceous plant characterized by leaves with a peculiar pungent taste and strong flavor. The crop, native to the Mediterranean Basin and western Asia, is widespread in all habitable continents as a leafy vegetable. Its consumption can be fresh as a topping on many dishes or m...

IMPSG 2021 1 st 2 nd july 2021

IMPSG 2021 1 st 2 nd july 2021

A rúcula (Diplotaxis spp. e Eruca spp.) é uma hortícola de folhas “baby-leaf” muito apreciada pelas suas características organoléticas e benefícios para a saúde. Pertence à família das Brassicáceas e a rúcula selvagem (Diplotaxis tenuifolia (L.) DC.) é a mais apreciada pelos consumidores. Nas últimas décadas, tem tido uma grande expansão e, atualme...

A rúcula (Diplotaxis spp. e Eruca spp.) é uma hortícola de folhas “baby-leaf” muito apreciada pelas suas características organoléticas e benefícios para a saúde. Pertence à família das Brassicáceas e a rúcula selvagem (Diplotaxis tenuifolia (L.) DC.) é a mais apreciada pelosconsumidores. Nas últimas décadas, tem tido uma grande expansão e, atualmen...

Five hundred mkicrosatellite loci were identified as partial result of a Diplotaxis tenuifolia (wild rocket) genome sequencing project and uploaded to the ncbi data base for use by the plant research comunity

105.7M spots - 15.3G bases. Genomic DNA sequenced using LLUMINA (Illumina HiSeq 1000). Leaf DNA was isolated after leaf tissue grinding under liquid nitrogen.

UALgDiploT.01 Organism name: Diplotaxis tenuifolia (eudicots)Isolate: DT550Sex: hermaphroditeBioSample: SAMN14589541BioProject: PRJNA624903Submitter: RemiruculaDate: 2020/09/30Assembly level: ScaffoldGenome representation: fullRefSeq category: representative genomeGenBank assembly accession: GCA_014822095.1 (latest)RefSeq assembly accession: n/aRef...

Rocket is a popular baby leaf vegetable from Brassicaceae family native from Mediterranean region appreciated for its organoleptic characteristics. Wild rocket (Diplotaxis tenuifolia L.) is characterized by distinct lobate leaves with a pungent aroma and typical bitter and spicy taste due to the presence of flavonoids and glucosinolates. In the las...

Downy mildew (DM; Hyaloperonospora brassicae) is one of the most serious diseases in radish and responsible for high production losses. The objectives of this study were (1) to identify new sources of DM resistance at the cotyledon stage; (2) to determine the inheritance of the resistance; and (3) to clarify the histological and cellular response i...

P19. New sources of resistance to downy mildew in a collection of wild and cultivated Brassicas Paula S. Coelho1, António A. Monteiro2, Violeta R. Lopes3, Ferdinando Branca4 1INIAV, Av. República, 2784-505 Oeiras, Portugal 2LEAF-ISA, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal 3INIAV-BPGV, Quinta de S. José, S. Pedro de Merel...

Brassica downy mildew (DM) is a foliar disease caused by the oomycete Hyaloperonospora brassicae (syn. Hyaloperonospora parasitica subs. brassica) that can affect plants at all stages including the nurseries and can limit the production of vegetable Brassica especially in temperate climates. Twenty-eight landraces and wild genotypes from different...

Wild (Diplotaxis tenuifolia) and cultivated (Eruca sativa) rocket are used in Mediterranean cuisine in fresh cut salads, being part of packaged ready-to-eat products and garnish for food. Baby-leaves are associated with a healthy diet style, showing twice more beneficial compounds when compared with other leaf types, and ensure the prevention of se...

The Brassica collection characterized by the COCHEVA BRAS project showed the main differences between Portuguese and Italian accessions in terms of biomorphological, biochemical and genetic traits, and allowed identifying the Most Appropriate Accessions (MAAs) to be proposed for inclusion into AEGIS. A great variability was observed for biochemical...

Downy mildew disease in vegetable brassicas (Brassica oleracea L.) caused by the obligate biotrophic Hyaloperonospora brassicae (formerly H. parasitica) is a limiting factor in European main production of temperate regions. Our group has identified and characterized various sources of resistance downy mildew with breeding value. Cotyledon inoculati...

Downy mildew disease in vegetable brassicas (Brassica oleracea L.) caused by the obligate biotrophic Hyaloperonospora brassicae (formerly H. parasitica) is a limiting factor in European main production of temperate regions. Our group has identified and characterized various sources of resistance downy mildew with breeding value. Cotyledon inoculati...

A doença do míldio no rabanete (Raphanus sativus L.) causada pelo oomiceta Hyaloperonospora brassicae é um dos principais fatores limitantes na produção de rabanete em regiões de clima temperado. Podem observar-se infeções de míldio em todos os estádios da cultura, dos cotilédones às raízes, com a formação de lesões escuras e esporulação do agente...

Downy mildew is an economically destructive disease of brassica crops in many growing regions throughout the world. Specialised pathogenicity of Hyaloperonospora species from different Brassica species and closely related ornamental or wild relatives has been described from host range studies. Pathotypic variation within amongst Hyaloperonospora br...

Downy mildew (DM) caused by the oomycete Hyaloperonospora brassicae (Gäum.) (Göker et al. 2003) affect Brassica oleracea L. plants, from seedlings in nurseries to field adult plants, reducing yield and severely compromising the quality of the marketed product. A dominant and monogenetically inherited DM resistance locus expressed at the adult plant...

We describe the construction of a BAC contig and identification of a minimal tiling path that encompass the dominant and monogenically inherited downy mildew resistance locus Pp523 of Brassica oleracea L. The selection of BAC clones for construction of the physical map was carried out by screening gridded BAC libraries with DNA overgo probes derive...

O míldio das brássicas é uma das principais doenças em regiões de clima temperado, causada pelo oomiceta Hyaloperonospora parasitica (Pers.:Fr.) Constant. As lesões de míldio danificam as folhas, parte comestível de diversos tipos de couve, e também as inflorescências da couveflor e da couve‑brócolo, desvalorizando‑as comercialmente. Em Portugal, p...

With 2 figures and 1 table Abstract We have previously constructed a genetic map of Brassica oleracea L. containing the Pp523 locus that confers downy mildew resistance to adult plants. In this work, 44 SSR markers of reference for the Brassica C genome chromosomes were added to the map, allowing the nine major linkage groups to be assigned to the...

Resumo O míldio das brássicas, causado pelo oomiceta Hyaloperonospora parasitica (Pers.: Fr.) Constant., é uma doença foliar frequente para a qual não existem formas de resistência eficazes que possam ser utilizadas em variedades comerciais. O trabalho consistiu no desenvolvimento de métodos de testagem da resistência das plantas, o que levou à ide...

1 O míldio do rabanete, provocado pelo oomiceta Hyaloperonospora parasitica, é a principal doença que afecta esta cultura. A regulamentação europeia relativa ao uso de produtos fitossanitários é cada vez mais restritiva, o que leva a que haja um interesse crescente no desenvolvimento de plantas que apresentem resistência durável ao míldio. Neste pr...

This work was a preliminary study focusing on membrane lipid composition of leaves of Brassica oleracea var. italica (broccoli) and var. tronchuda (tronchuda cabbage) populations differing in adult plant downy mildew (DM) resistance. Plants with different DM response were selected in a field experiment with F3 families from a cross between a suscep...

Exploiting the Brassica/Arabidopsis genomic synteny for BAC contig construction.

Downy mildew (DM), is a disease incited by the oomycete Hyaloperonospora parasitica (Pers:Fr) that causes stunting and death of plants at the seedling stage and reduces yield and lowers the quality of adult plants in Brassica oleracea L. An accession of the portuguese cabbage “couve Algarvia” (Brassica oleracea var tronchuda) was found to be resist...

A dense genetic map of molecular markers, covering 89.5% of the Brassica oleracea L genome and containing the dominant downy mildew resistance locus Pp523 was previously constructed in our laboratory (Farinhó et al, 2004). The aim of the present work was to assign the linkage groups of our map to the 9 real physical chromosomes of B. oleracea, usin...

Downy mildew caused by the oomycete Hyaloperonospora parasitica (formerly Peronospora parasitica) is a worldwide foliar disease of Brassica vegetables, which may cause seedling loss in the nurseries and damage to adult plants in the field. Disease symptoms start from the lower leaves and progress upwards. Three experiments were conducted under cont...

Desenvolveu-se um método de avaliação da resistência em laboratório em que se avaliaram discos foliares colhidos em plantas adultas, em diversas posições no caule, que foram depois inoculados adaptando-se os procedimentos seguidos nos testes de resistência cotiledonar. Avaliou-se igualmente o interesse da aplicação de Benziladenina (6-BA) a 0,5 ppm...

Based on the sequences of markers OPK17 980 , ScJ19/Pag1, ScR15 and AGC.CAT 300 we have designed overgo probes used for screening two B. oleracea BAC genomic libraries (work performed at Dr. Andrew Paterson's Plant Genome Mapping Laboratory (PGML), University of Georgia, USA) The PGML data regarding the screening of BAC libraries with B. oleracea o...

We recently mapped the Pp523 locus that includes a single, dominant gene conferring resistance to downy mildew expressed in adult plants to a 75.1cm long linkage group on a genetic linkage map of Brassica oleracea L. More recently, we identified a new AFLP marker 2.8cm downstream from the resistance gene. The five DNA markers within an 8.5cm region...

Downy mildew (DM) caused by the oomycete Peronospora parasitica (Pers. ex Fr.) Fr. is an important foliar disease of Brassica vegetables worldwide especially in temperate climates, which may cause the lost of seedlings in the nurseries and the damage of adult plants in the field. A gradient of disease symptoms can be observed in the plants when an...

Two novel sources of genetic resistance to downy mildew in adult plants of Brassica oleracea L., Pp523 and Pp1A, were recently identified. Both resistances proved to be monogenic, dominant, and independently inherited. Starting from a recently published genetic map (Farinhó et al., 2004) where the Pp523 locus was already mapped, some additional DNA...

No âmbito do projecto PAMAF-IED ‘Valorização da qualidade dos produtos tradicionais do Norte Alentejano’ surgiu o interesse de recuperar um melão tardio, do tipo ‘Tendral’, com elevado poder de conservação e que tinha sido ensaiado e produzido na região de Elvas nos anos 70 do século passado. Aquela variedade de melão foi melhorada na Estação Agron...

The progeny of several crosses between the resistant Couve Algarvia of Brassica oleracea var. tronchuda Bailey and susceptible hosts was evaluated with the objective of determining the mode of inheritance of resistance to downy mildew (DM) (Peronospora parasitica (Pers. ex Fr.) Fr.) at cotyledon and adult-plant stages and to find out whether the re...

O míldio causado pelo oomiceta Peronospora parasitica (Pers. ex Fr.) Fr. infecta plantas de brássica em todo o mundo, causando elevados prejuízos, tanto nas plantas jovens nos viveiros em estufa, como nas plantas adultas no campo. Estudos anteriores demonstraram a existência de variedades de brássica de origem portuguesa resistentes ao míldio, como...

The identification of the gene Pp523, conferring downy mildew resistance to adult plants of broccoli (Brassica oleracea convar. italica), led to the construction of a genetic map that included this resistance locus, 301 amplified fragment length polymorphisms, 55 random amplified polymorphic DNAs, 46 inter-simple sequence repeats, three simple sequ...

Os estudos de resistência ao míldio das crucíferas (Peronospora parasitica (Pers. ex Fr.) Fr.) em plântulas no estado cotiledonar, em plantas jovens e em plantas adultas de Brassica oleracea L. apresentam por vezes resultados contraditórios, não tendo sido ainda estabelecida uma relação entre a resistência nos diversos estados de desenvolvimento da...

The objective of this study was to determine the mode of inheritance of field resistance to downy mildew (Peronospora parasitica (Pers. ex Fr.) Fr.) in broccoli (Brassica oleracea var.italica) at the adult plant stage. The F1, F2 and F3 progeny of resistant and susceptible plants of broccoli were tested in the field under natural infection, in cent...

This research compared the expression of resistance to downy mildew at cotyledon and adult plant stages in seven Brassica oleracea genotypes against two P. parasitica isolates of different virulence. Seven day old seedlings were dual inoculated under controlled environment by depositing two 10 μl droplets of a spore suspension of a different isolat...

The objective of this study was to determine the mode of inheritance of field resistance to downy mildew (Peronospora parasitica (Pers. ex Fr.) Fr.) in broccoli (Brassica oleracea var. italica) at adult-plant stage. The F1, F2 and BC progeny of resistant (line A) and susceptible (line B) plants of broccoli were tested in the field under natural inf...

No campo, os ataques de míldio das crucíferas em plantas adultas de Brassica oleracea, causado pelo fungo Peronospora parasitica (Pers. ex Fr.) Fr., iniciam-se pelas folhas da base, progredindo para as folhas superiores. Este padrão de ataque dificulta a avaliação da resistência genética dos indivíduos em programas de melhoramento, pois não está de...

410 accessions of a "core collection" of Brassica oleracea were screened at the cotyledon stage for resistance to downy mildew with isolates P501 (Portugal), P005 and P006 (both UK). Seedlings were assigned to one of six previously described interaction phenotypes. Seed of 20 accessions that showed a high proportion of seedlings in non-sporulating...

The nitrogen fertilization is a strong instrument in controlling vegetable production as it directly influences canopy growth and development, light interception, distribution of assimilates and cultural cycle duration. The main goal of this work is the study of the potato plant response to three levels of nitrogen fertilization.