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Nowadays the use whole cell or enzymes in modern chemical manufacturing processes is gaining ground over chemical synthetic processes. Traditional chemical methods often require time-consuming multistep-processes, harsh operating conditions and the use of chemical reagents and organic solvents that are expensive and environmentally harmful. Conversely, enzymatic production by whole cells or enzymes improves regio-, stereo- and enantioselectivity and provides an eco-friendly framework. As a result, the replacement of chemical strategies by enzymatic or chemo-enzymatic approaches is a common practice in pharmaceutical, textile, food, cosmetic and biomedical industries. In this respect, this Special Issue aims to gather the recent advances in the field of biocatalysis and biotransformations. https://www.sciencedirect.com/journal/biochimica-et-biophysica-acta-bba-proteins-and-proteomics/special-issue/10XXWKCMZF5 Topics include but are not limited to the following - Directed evolution - Protein engineering - Bioprocesses catalyzed by proteins or whole cells - Enzyme immobilization - Bioprocess Engineering - Enzymatic and/or chemoenzymatic synthesis of high value-added compounds - Applied Biotechnology - Environmental Biocatalysis - Agro- and Food Biotechnology - Industrial Biotechnology