Paul James

Paul James
Northumbria University · Hub for Biotechnology in the Built Environment

BSc Biological and Medicinal Chemistry

About

10
Publications
1,087
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112
Citations
Introduction
My lab is looking to use Synthetic Biology techniques to develop microbial technologies that can act as a buildings metabolism - processing waste and generating energy and other high value products. We will be looking to develop tool boxes that allow us to take non model bacteria and use them as cell factories. I am also interested in the enzymes we will need to use to break down waste and produce high value products.
Additional affiliations
January 2018 - May 2020
University of Exeter
Position
  • Fellow
Education
September 2006 - September 2010
University of Exeter
Field of study
  • Biological Sciences

Publications

Publications (10)
Article
Full-text available
A novel transketolase has been reconstituted from two separate polypeptide chains encoded by a ‘split-gene’ identified in the genome of the hyperthermophilic bacterium, Carboxydothermus hydrogenoformans. The reconstituted active α2β2 tetrameric enzyme has been biochemically characterized and its activity has been determined using a range of aldehyd...
Article
Full-text available
Science is key to developing sustainable products and solutions. But scientists also need to work more with governments, industry and society to help implement those solutions.
Article
Well-characterised promoter collections for synthetic biology applications are not always available in industrially relevant hosts. We developed a broadly applicable method for promoter identification in atypical microbial hosts that requires no a priori understanding of cis-regulatory element structure. This novel approach combines bioinformatic f...
Article
Full-text available
Two new thermophilic branched chain amino acid transaminases have been identified within the genomes of different hyper-thermophilic archaea, Geoglobus acetivorans, and Archaeoglobus fulgidus. These enzymes belong to the class IV of transaminases as defined by their structural fold. The enzymes have been cloned and over-expressed in Escherichia col...
Article
Full-text available
Thermoanaerobacter ethanolicus JW 200 has been identified as a potential sustainable biofuel producer due to its ability to readily ferment carbohydrates to ethanol. A hybrid sequencing approach, combining Oxford Nanopore and Illumina DNA sequence reads, was applied to produce a single contiguous genome sequence of 2,911,280 bp.
Article
Plant polyphenols are known to have varying antimicrobial potencies, including direct antibacterial activity, synergism with antibiotics and suppression of bacterial virulence. We performed the in vitro oligomerization of resveratrol catalyzed by soybean peroxidase, and the two isomers (resveratrol-trans-dihydrodimer and pallidol) produced were tes...
Article
Full-text available
Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide to bicarbonate. A thermophilic Thermovibrio ammonificans α-carbonic anhydrase (TaCA) has been expressed in Escherichia coli and structurally and biochemically characterized. The crystal structure of TaCA has been determined in its native form and in two complexes with bo...
Chapter
Considerable interest has been generated in the mechanism which nature utilises to increase the stability of enzymes found in thermophilic and hyperthermophilic species. This has been the subject of many reviews, and our understanding has been enhanced by the increasing number of high-resolution thermostable enzyme structures that have been determi...

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Projects

Project (1)
Project
Industrial Research Fellow in the BioEconomy Centre