Patrizia Casagrande Proietti

Patrizia Casagrande Proietti
Università degli Studi di Perugia | UNIPG · Department Veterinary Medicine

About

60
Publications
17,303
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721
Citations
Citations since 2016
29 Research Items
620 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140

Publications

Publications (60)
Article
Full-text available
The spread of resistant bacteria from livestock to the food industry promoted an increase of alternative poultry production systems, such as organic and antibiotic-free ones, based on the lack of antimicrobial use, except in cases in which welfare is compromised. We aimed to investigate the antibiotic susceptibility of commensal Escherichia coli is...
Raw Data
E. coli è sicuramente reputata tra le specie batteriche in cui più rapidamente si è verificata la selezione di geni di resistenza a seguito dell’uso di antimicrobici (Tadesse et al., 2012). Questo microrganismo, a causa della sua elevata diffusione, è considerato anche un indicatore dell’antibiotico-resistenza delle popolazioni di gram negativi e u...
Article
Full-text available
This work aimed to evaluate the antimicrobial susceptibility of 87 Salmonella Infantis strains isolated in Italy from 2016 to 2019 along the food chain of broiler meat production and in humans and to determine the genetic profiles of the strains in order to establish a possible correlation with the antimicrobial pattern. All isolates were tested by...
Article
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As a result of public health concerns regarding antimicrobial resistance in animal-based food products, conventional poultry companies have turned to ‘raised without antibiotics’ (ABF) and organic farming systems. In this work, we evaluated the influence of rearing systems on antimicrobial susceptibility in E. coli and extended-spectrum β-lactamase...
Article
Full-text available
This study investigated the effects of a short-term administration of bovine colostrum (BC) in a TNBS model of induced colitis. Colitis was induced by TNBS treatment after seven days of BC (BC group, n = 12) or saline (control group, n = 12) administration in mice. Clinical signs, histopathological characteristics, expression levels of Toll-like re...
Article
Full-text available
Among oregano properties, its antioxidant and antibacterial effects are particularly interesting. Oregano is also able to induce a higher glycoconjugate production in gut, creating a physical barrier against microorganisms. This study evaluated the effects of adding an aqueous extract of oregano (OAE) to the diet of two homogenous groups of pigs du...
Article
The objectives of this work were to evaluate β-lactamase-mediated β-lactam resistance in Campylobacter coli and Campylobacter jejuni isolates obtained from broiler chickens, expression of the blaOXA-61 gene in relation to β-lactamase production, and the possible association between blaOXA-61 gene expression and the action of inhibitors when combine...
Article
Full-text available
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder for which the current medical therapy is not completely effective. Bovine colostrum (BC) is a biological fluid rich in bioactive molecules that may have beneficial effects on several gastrointestinal disorders. The objectives of this study were to assess the preventive effects of B...
Data
Disease activity index. Parameters and criteria for scoring Disease activity index. (PDF)
Data
Estimated marginal means ± standard error (SE) and P-values for TNBS (2 levels: pre- and post- TNBS treatment) and Group (2 levels: Control and BC groups) effects of bacterial counts (log10 CFU per gram of colon samples). (PDF)
Data
Experimental Protocol. After an acclimatization period of 10 days, 24 CD-1 male mice were randomly divided into two groups (n = 12) and daily received by gavage a suspension containing bovine colostrum (BC group) or the same volume of saline solution (CN group) for 21 days. After 21 days, 6 mice of each group (BC pre-TNBS and CN pre-TNBS) were sacr...
Data
The concentrations of Ig and other major ingredients of BC. (PDF)
Article
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This study investigated the presence and the level of Campylobacter spp. contamination in 41 thigh samples (with skin) and 37 skinless breast samples collected at the end of slaughter (T1) and after 10 day period at refrigeration temperature (4°C) (T2), corresponding to their commercial shelf life. The isolates were phenotypically classified as Cam...
Article
Full-text available
Testudinid herpesviruses (TeHVs) have a worldwide distribution among tortoises. However, information such as risk factors promoting the occurrence or the recrudescence of the associated disease and the mid-term sequelae of an outbreak comprising the extent and dynamic of the viral shedding have been only minimally investigated. Critical management...
Data
PCR protocols used for herpesviruses detection in tortoises. (DOC)
Data
TeHV3 infected tortoise with neurological signs. This female of Testudo hermanni, over 30 years old, had neurological signs, consisting of hind limb paresis that lasted all year long. At the onset of the paresis, the tortoise was able to lay 3 eggs. It then partially recovered from the neurological syndrome, but died during post-hibernation of the...
Data
Alignment of the partial sequences of TeHV3 ribonucleotide reductase-large subunit, UL39 gene, obtained from tortoises nos. 6, 18 (including both the 2011 and 2013 samplings) and from the positive hatchling. The alignment obtained by MAFFT software shows full nucleotide identity among the compared sequences. (TIF)
Data
Map of the enclosures where chelonians were located. (TIF)
Data
Investigation on the possible effects of the temperature during the TeHV3 outbreak and proposal for a statistical model. (DOCX)
Article
The aim of the present study was to investigate the antibiotic resistance and bio lm formation among a collection of 51 clinical isolates of Staphylococcus pseudintermedius collected from canine pyoderma. All isolates were tested for the susceptibility to a panel of 14 antimicrobial agents by the disk di usion method in Müeller-Hinton agar. Oxacill...
Article
Full-text available
Helicobacter suis is the second most prevalent Helicobacter species in the stomach of humans suffering from gastric disease. This bacterium mainly inhabits the stomach of domesticated pigs, in which it causes gastric disease, but it appears to be absent in wild boars. Interestingly, it also colonizes the stomach of asymptomatic rhesus and cynomolgu...
Article
Aims: Genetic diversity and antimicrobial resistance of C. coli and C. jejuni were investigated along the broiler chicken production chain in central Italy. Methods and results: Campylobacter spp. isolated from cloacal swabs in farms (n = 116) and from neck skin of chilled and eviscerated carcasses at slaughter (n = 24) were identified as C. col...
Article
The mammalian commensal microbiota con- stitutes over a thousand bacterial phylotypes (Suchodolski, 2014) and confers important func- tions, including a mucosal barrier function, a metabolic function, and an immune regulatory function, which contribute to the development and regulation of the gut immune system (Backhed et al., 2005). The compositio...
Article
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Microbiological and parasitological investigation was carried out on a colony of feral pigeons, located in a green area near the main hospital of a Central Italy city. One hundred pigeons were submitted to clinical examination. Cloacal swabs, grouped in pool of 4 samples, were analyzed to detect the presence of Coxiella burnetii, Chlamydia psittaci...
Article
One hundred and seventy one-day-old female broiler chicks were randomly divided into three groups fed with different dietary treatments: basal control diet (C); C supplemented (2 g/kg) with an oregano aqueous extract (O); C supplemented (150 mg/kg) with vitamin E (E). Growth performance was evaluated at 21 (T1) and 42 days (T2). On the same days, m...
Article
Inflammatory bowel diseases (IBD) are characterized by inflammatory conditions of the intestine. Probiotic bacteria (PB) can have beneficial effects in several gastrointestinal disorders. The objectives of this study were: (i) to provide an acute experimental IBD model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in CD-1 mice, and (ii) to a...
Article
Full-text available
This paper reviews the composition of colostrum and the potential preventive and therapeutic use of this "first milk" for treating various gastrointestinal disorders in humans. Colostrum is a complex biological liquid that is richer in antimicrobial peptides, immune-regulating compounds and growth factors than the subsequent mature milk. The main f...
Article
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The current study investigated the effects of Lactobacillus acidophilus and Bacillus subtilis, used as probiotics, on the microflora, morphology, and morphometry of the gut in organic laying hens. The birds (180 Hy-Line laying hens) were divided into 3 homogenous groups and received a pre-deposition diet from 16 to 20 wk of age and a deposition die...
Article
Full-text available
The objective of this study was to evaluate the changes in some bacterial species of colonic microbiota, the clinical signs and the intestinal changes in mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. CD-1 male mice were randomly divided into three groups and inoculated intrarectally with saline, ethanol or TNBS solutions. Eth...
Article
Full-text available
A 57-day study was performed to determine the effects of two aqueous extracts (AEs) on broiler performance, immune function and intestinal microflora. Four groups of 75 one-day-old female broilers (Ross308) received one of the following treatments: (1) a standard commercial feed (C); (2) C supplemented with 2 g/kg rosemary AE (R); (3) C supplemente...
Article
Full-text available
Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence te...
Article
Early diagnosis and prevention of Rhodococcus equi pneumonia in foals represent important goals for equine clinicians. Recent protocols for preclinical detection and treatment of Rhodococcosis in foals typically rely on a multimodal diagnostic approach based on sonographic evidence suggestive of pyogranulomas, sonographic abscess scores and laborat...
Article
In recent years, there has been increasing evidence of the potential pathogenic significance of equine gammaherpesviruses in the horse. In humans, cattle and mice, gammaherpesviruses have already been associated with uterine infection. The aim of the present study was to investigate the presence of gammaherpesviruses in uterine flushings of mares w...
Article
Full-text available
Ascending infections of equine uterus frequently result in placentitis and abortions; most of these infections are bacterial and are less commonly due to fungi. This report describes an abortion case in an Arab mare due to Candida guilliermondii that was diagnosed via cytological, histological, cultural and biomolecular assays. The histological les...
Article
The role of the horse in Coxiella burnetii infection has not been defined. Accordingly, a twofold approach was taken to further our knowledge on this topic: (1) conduct a systematic review of the literature to establish available evidence of C. burnetii infection in the horse; (2) undertake a biomolecular investigation of 122 cases of equine aborti...
Article
Full-text available
Coxiella burnetii is an obligate intracellular bacterium, causing a zoonosis called Q fever. The detection of this etiological agent and its epidemiology in an area is important to estimate the risk of infection. Ruminants are the main source of the infection and sheep are considered very important for the spread of the agent, by birth products, bu...
Article
Full-text available
Herpesviral infections frequently occur in horses. The objective of this study was to investigate the possible association of equine herpesviruses (EHV-1, EHV-2, EHV-3, EHV-4, EHV-5) with other causes of abortion, neonatal mortality or placental disorder. Sixty-seven abortions, 22 stillbirths, 14 cases of neonatal foal mortality and 3 cases of plac...
Article
Staphylococcus pseudintermedius is the most frequent staphylococcal species isolated from canine pyoderma. The control of S. pseudintermedius infection is often difficult due to the expanded antimicrobial resistance phenotypes. Antibiotic resistance in staphylococcal pathogens is often associated to mobile genetic elements such as the insertion seq...
Article
Full-text available
The aim of this study was to determine serological values of lysozyme, hemolytic complement levels (alternative pathway), and bactericidal activity of serum in turkeys kept in different rearing systems (industrial, backyard, and experimental). Results showed that the values for serum bactericidal activity and hemolytic complement levels increased w...
Article
The objective of this manuscript was to validate published PCR-based methods for detection of β-haemolytic Streptococci by comparison with established bacteriological techniques using 85 clinical isolates recovered from uterine swabs of mares with clinical signs of endometritis and to determine the distribution of SeeL/SeeM and SzeL/SzeM superantig...
Article
Full-text available
Feline Rhodococcus equi infection is rare, despite the bacteria is widespread in the environment. R equi infection is typically observed in equine species but the infection has also been reported in dogs, cats and other domestic animals. There are a few reports regarding pulmonary R equi infection in cats and the disease appears to be limited to th...
Article
Full-text available
The aim of this study was to evaluate the genotypic characteristics of Staphylococcus aureus isolates (n=170) from bovine milk collected from seven dairy farms in Italy. On the basis of cultural and biochemical properties and by amplification of the 23S rRNA specific to S. aureus, all isolates were identified as S. aureus. To genotypically characte...
Article
Full-text available
The aim of this study was to evaluate the presence of Helicobacter (H.) spp. in swine affected by gastric ulceration. Stomachs from 400 regularly slaughtered swine were subjected to gross pathological examination to evaluate the presence of gastric ulcers. Sixty-five samples collected from ulcerated pars esophagea and 15 samples from non-ulcerated...
Article
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This is the first paper describing the cloning of pigeon IFN-γ gene (PiIFN-γ) and the analysis of the in vitro expressed recombinant protein. The PiIFN-γ gene was identified by RT-PCR as a 498bp, fragment coding for a precursor protein of 165 amino acids instead of 164 amino acids, as observed in the other avian species. The recombinant protein was...
Article
Full-text available
The present study was conducted to evaluate in field the efficacy of an additive (SOP® C POULTRY), as an agent for the control of micro-organisms in broiler litter. The Total aerobic Microbial Count (TMC), Staphylococcus species (spp.), Coliforms, and Salmonella spp. in broiler litter samples of both the Houses, 2 (H2) and 3 (H3), were determined,...
Article
Full-text available
Adrenocortical and thyroidal hormones are physiological indicators of various forms of stress in the fowl. In order to establish possible variations in corticosterone levels, blood samples were collected from ISA Brown hens reared in three different housing systems (cage, floor and organic way). Results showed that corticosterone concentrations wer...
Article
Full-text available
This paper describes a case of an unusual recovery of adult Ascaridia galli in hen’s egg. Several data are available on this occurrence but it appears to be the first case described in Italy. The worm was identified as an adult female, 6.8 cm in length, with three trilobed lips, cervical narrow alae, oesophagus club-shaped without posterior bulb, v...
Article
Full-text available
The aim of this study was to evaluate the possible differences in the intestinal microflora composition among the different rearing systems (conventional vs organic) and the Salmonella diffusion using bacteriological techniques. The results showed that the differences between the two groups at the same age, expressed by the bacterial count, are not...
Article
Full-text available
The major bacteria colonizing the intestinal tract (ileum and caecum) of organic (O) and conventional (C) chickens were counted, isolated and identified by conventional methods. Chickens were obtained from 7 conventional and 7 organic chicken farms (n=203). Intestinal sampling was performed at different ages, every 10 days, starting at 20 days unti...
Article
Full-text available
The progression of some parameters of natural immunity in meat turkey reared outdoors was investigated. The levels of the haemolytic complement were lower than those obtained in commercial turkeys and in turkeys reared in a controlled environment during one of our previous investigations. The weather conditions could have influenced the trend of th...
Article
Full-text available
The broiler chickens, especially if intensively reared, can be considered as an important reservoir of Salmonella infections in humans. Many consumers assume that broiler chickens, grown under conventional commercial conditions, have higher infection levels of Salmonella than free-range organic chickens. The subject of this study was firstly to ass...
Article
Salmonellosis in humans is still the leading food-borne infection in industrialised countries. Modification in poultry production systems by affecting animal density, feeding system, animal welfare as well as pharmacological treatments are expected to modify the germ colonization of intestine. In particular their influence on the occurrence of Salm...
Article
Full-text available
The aim of this study was to count, isolate and identify by traditional methods the major bacteria colonizing the intestinal tract of organic and conventional chickens. The study was performed in 2 conventional and 2 organic chicken farms. Intestinal samples (24 from ileum and 24 from caecum) were collected from organic reared chickens during the p...
Article
This paper describes a polymerase chain reaction (PCR)-based method performed on blood samples and intestinal content to detect subclinical pigeon circovirus (PiCV) infection in live pigeons. In addition, two sets of primers (primer set 1 and 2), designed in two different regions of the viral genome, were used to provide evidence of possible differ...
Article
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This paper describes an outbreak of disease caused by Hafnia alvei in pullets. Cloudy swelling and the fatty degeneration of liver associated with splenic lymphocytic depletion were the most preminent lesions. The organism was identified by biochemical tests and by a Hafnia-specific bacteriophage test. Laying hens and pullets were infected experime...
Article
Full-text available
SOMMARIO Le emissioni di sostanze odorigene da allevamenti zootecnici sono generate dalla degradazione macrobiotica delle deiezioni animali e quindi imputabili a processi naturali. Tuttavia, tali emissioni possono essere causa sia di disagio per l'ambiente circostante in quanto poco tollerabili dal punto di vista olfattivo, sia di problemi per salu...

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