Patrizia Bogani

Patrizia Bogani
University of Florence | UNIFI · Dipartimento di Biologia

About

116
Publications
9,236
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1,320
Citations
Citations since 2016
35 Research Items
547 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140

Publications

Publications (116)
Article
Full-text available
Acmella oleracea L. is an edible and medicinal plant commonly known for its local anaesthetic effect induced by the alkylamide spilanthol. It is also rich of secondary metabolites of biological interest, mainly phenolic acids and glycosylated flavonoids. This study evaluated for the first time alkylamides and phenolic compounds in aerial parts and...
Article
Full-text available
Paper-based biosensors featuring immunoconjugated gold nanoparticles have gained extraordinary momentum in recent times as the platform of choice in key cases of field applications, including the so-called rapid antigen tests for SARS-CoV-2. Here, we propose a revision of this format, one that may leverage on the most recent advances in materials s...
Preprint
Full-text available
Background Paper-based biosensors featuring immunoconjugated gold nanoparticles have gained extraordinary momentum in recent times as the platform of choice in key cases of field applications, including the so-called rapid antigen tests for SARS-CoV-2. Here, we propose a revision of this format, one that may leverage on the most recent advances in...
Article
Full-text available
Echinacea purpurea is a plant cultivated worldwide for its pharmaceutical properties, mainly related to the stimulation of the immune system in the treatment of respiratory infections. The cypselas (fruits) of E. purpurea were examined in order to investigate the presence, localization and potential function(s) of endophytic microorganisms. Electro...
Article
Full-text available
Medicinal plants (MPs) have been used since antiquity in traditional and popular medicine, and they represent a very important source of bioactive molecules, including antibiotic, antiviral, and antifungal molecules. Such compounds are often of plant origin, but in some cases, an origin or a modification from plant microbiota has been shown. Actual...
Article
Full-text available
Acmella oleracea L. is an important medicinal plant, commonly known as the toothache plant. It is a rich source of secondary metabolites used for the treatment of different human disorders. The demand for Acmella oleracea L. has increased due to its putative health benefits (in terms of both biomass quantity and bioactive compound purification). In...
Article
The differences in volatile profile of Echinacea purpurea plants not-inoculated (EpC) and inoculated with their endophytes from roots (EpR) and stem/leaves (EpS/L) were analysed and compared by GC-FID/GC-MS in an in vitro model. Non-terpenes and sesquiterpene hydrocarbons were the most abundant classes with an opposite behaviour of EpS/L showing a...
Article
Histidine biosynthesis is one of the most characterized metabolic routes for its antiquity and its central role in cellular metabolism; indeed, it represents a cross-road between nitrogen metabolism and de novo synthesis of purines. This interconnection is due to the activity of imidazole glycerol phosphate synthase, a heterodimeric enzyme constitu...
Article
Full-text available
The insurgence of antibiotic resistance and emergence of multidrug-resistant (MDR) pathogens prioritize research to discover new antimicrobials. In this context, medicinal plants produce bioactive compounds of pharmacological interest: some extracts have antimicrobial properties that can contrast different pathogens. For such a purpose, Origanum vu...
Article
The stabilization of the optical properties of anisotropic plasmonic particles upon thermal annealing and laser irradiation is an important issue in many biomedical applications. Here, we address the effect of small thiols on the thermal and photo-stability of gold nanorods. As-synthesized colloids were treated with mixtures of thiolated polyethyle...
Article
The role of the interaction(s) between medicinal plants (MPs) and their endophytes (bacterial microbiome) in the production of bioactive compounds (BCs) with therapeutic properties is emerging. Here, we propose Echinacea purpurea (L.) Moench as a new model to reveal the intimate crosstalk between MPs and bacterial endophytes, aiming to discover (ne...
Article
Full-text available
The controversial anti-proliferative effects of Echinacea purpurea (L.) Moench (Asteraceae) might be related to different plant metabolites contained in plant samples, extracts and products. The influence of bacterial endophytes on the synthesis of bioactive compounds in the medicinal plants has been previously demonstrated but there are only few s...
Article
Full-text available
Background Echinacea-endophyte interaction might affect plant secondary metabolites content and influence bacterial colonization specificity and plant growth, but the underlying mechanisms need deepening. An in vitro model, in which E. purpurea axenic plants as host species and E. angustifolia and Nicotiana tabacum as non-host species inoculated wi...
Article
A key factor in the study of plant-microbes interaction is the composition of plant microbiota, but little is known about the factors determining its functional and taxonomic organization. Here we investigated the possible forces driving the assemblage of bacterial endophytic and rhizospheric communities, isolated from two congeneric medicinal plan...
Article
Full-text available
The influence of the interaction(s) between the medicinal plant Echinacea purpurea (L.) Moench and its endophytic communities on the production of alkamides is investigated. To mimic the in vivo conditions, we have set up an infection model of axenic in vitro E. purpurea plants inoculated with a pool of bacterial strains isolated from the E. purpur...
Article
Oregano essential oils (EOs) could represent interesting therapeutic strategies to treat multidrug-resistant (MDR) pathogens as Pseudomonas aeruginosa, responsible for respiratory infections in cystic fibrosis (CF) patients. There could be a great variability in EOs composition when extracted from different plant species. The aim of this study was...
Article
Full-text available
We announce here the draft genome sequence of Arthrobacter sp. strain EpSL27, isolated from the stem and leaves of the medicinal plant Echinacea purpurea and able to inhibit human-pathogenic bacterial strains. The genome sequencing of this strain may lead to the identification of genes involved in the production of antimicrobial molecules.
Article
Full-text available
In this announcement, we detail the draft genome sequence of the Pseudomonas sp. strain Ep R1, isolated from the roots of the medicinal plant Echinacea purpurea . The elucidation of this genome sequence may allow the identification of genes associated with the production of antimicrobial compounds.
Article
Nicotiana is a well characterized genus of Solanaceae. It is widely studied in literature in different research fields, such as biology, environmental chemistry, etc. In this study Nicotiana langsdorffii wilde type plants and transgenic for the rat glucocorticoid receptor gene (GR) and Agrobacterium rhizogenes rolC gene were used to value the effec...
Article
This study reports the impact of an integral glucocorticoid expression system (GR-system) on plant and plant-nematode reproduction. Seedlings expressing a GR-system (transGR+) show affections of growth and development. It is well-known that plant-hormones such as gibberellins, auxins and steroids are essential for developmental and physiological pr...
Article
In recent years, there has been increasing interest in plant microbiota; however, despite medicinal plant relevance, very little is known about their highly complex endophytic communities. In this work, we report on the genomic and phenotypic characterization of the antimicrobial compound producer Rheinheimera sp. EpRS3, a bacterial strain isolated...
Article
Full-text available
The last version of Arthrobacter sp. EpRS66 genome has a total length of 3,707,708 bp and embeds only 2 scaffolds (L50 equal to 1), with a mean G+C content of 59.27%. The annotation analysis identified a total of 3485 genes, of which 3383 have been annotated as coding DNA sequences (CDS), 29 as pseudogenes, 4 as rRNAs, 68 as tRNAs, and 1 as ncRNA....
Article
Full-text available
One of the major unanswered questions with respect to the commercial use of genetic transformation in woody plants is the stability of the transgene expression over several decades within the same individual. Gene expression is strongly affected by the copy number which has been integrated into the plant genome and by the local DNA features close t...
Article
Full-text available
Essential oils (EOs) are known to inhibit the growth of a wide range of microorganisms. Particularly interesting is the possible use of EOs to treat multidrug-resistant cystic fibrosis (CF) pathogens. We tested the essential oil (EO) from Origanum vulgare for in vitro antimicrobial activity, against three of the major human opportunistic pathogens...
Article
Full-text available
We announce here the draft genome sequence of Pseudomonas sp. strain EpS/L25, isolated from the stem/leaves of the medicinal plant Echinacea purpurea . This genome will allow for comparative genomics in order to identify genes associated with the production of bioactive compounds and antibiotic resistance.
Article
In this study wild type Nicotiana langsdorffii plants were genetically transformed by the insertion of the rat gene (gr) encoding the glucocorticoid receptor or the rolC gene and exposed to water and heat stress. Water stress was induced for 15 days by adding 20% PEG 6000 in the growth medium, whereas the heat treatment was performed at 50 °C for 2...
Article
Full-text available
The present study reports evidence of the pleiotropic effects caused by the insertion of the rat glucocorticoid receptor (GR) into the genome of Nicotiana langsdorffii. Transgenic N. langsdorffii-GR plants and the wild-type genotypes were analysed for their phenotypic and physiological characteristics. The integration of the GR gene affected flower...
Article
Full-text available
Nicotiana Langsdorffii plants, wild and transgenic for the Agrobacterium rhizogenes rol C gene and the rat glucocorticoid receptor (GR) gene, were exposed to different abiotic stresses (high temperature, water deficit and high chromium concentrations). An untargeted metabolomic analysis was carried out in order to investigate the metabolic effects...
Article
Full-text available
The genus Gypsophila contains about 150 annual and perennial flowering plant species native to the temperate regions of Europe and Asia. Nowadays Gypsophila species are present worldwide as garden ornamental plants. Although Gypsophila is one of the most economically important ornamental crops, little is known about its genetic variability and the...
Article
Full-text available
Recently our findings have shown that the integration of the gene coding for the rat gluco-corticoid receptor (GR receptor) in Nicotiana langsdorffii plants induced morphophysiological effects in transgenic plants through the modification of their hormonal pattern. Phytohormones play a key role in plant responses to many different biotic and abioti...
Article
Full-text available
The World Anti-Doping Agency fears the use of gene doping to enhance athletic performances. Thus, a bioanalytical approach based on end point PCR for detecting markers' of transgenesis traceability was developed. A few sequences from two different vectors using an animal model were selected and traced in different tissues and at different times. In...
Article
Full-text available
In the present study, we analysed the effect of integration of single Agrobacterium rhizogenes rol genes or their combinations in the rol-containing Nicotiana glauca, in the non-rol Nicotiana langsdorffii, in their tumorous hybrid N. glauca×N. langsdorffii and in a non-tumorous mutant of it. We investigated whether the integration of transgenes cou...
Article
Surface plasmon resonance imaging (SPRi) was used as the transduction principle for the development of optical-based sensing for transgenes detection in human cell lines. The objective was to develop a multianalyte, label-free, and real-time approach for DNA sequences that are identified as markers of transgenosis events. The strategy exploits SPRi...
Conference Paper
Introduction: The growth and development of plants and animals are coordinated at the tissue and organ level by signalling molecules involved in intercellular communication. Many steroid molecules have been identified as essential growth regulators in plants as well as in animals. Two main modes of steroid action are known: (1) the rapid non-genomi...
Article
Full-text available
Characterization of plant germplasm using molecular techniques is playing an increasingly important role in the management of plant genetic resources. Genetic diversity and relationships between local cultivars from two important Italian olive growing regions, Sicily and Calabria, were studied by means of ISSR markers. As ISSR technique allows the...
Article
Full-text available
The analytical performances of two optimized analytical methodologies used for the determination of auxins, cytokinins, and abscisic acid in plant samples were critically compared. Phytohormones were extracted from Nicotiana glauca samples using a modified Bieleski solvent and determined both by gas chromatography-mass spectrometry (GC-MS), after d...
Article
Sports authorities fear that a new form of doping called gene doping, based on the misuse of gene therapy, represents an emerging important problem and so far no methods are available for detecting it. The World Anti-Doping Agency (WADA) has included since 2003 for the first time gene doping methods in the "Prohibited List of Substances and Methods...
Article
The development of analytical methods for genetically modified organisms (GMO) screening is of great interest. In particular, since even highly processed GMO-derived food products are covered by new European legislations, a great effort has been devoted to the application of the analytical tests to these products.This work describes a polymerase ch...
Article
Full-text available
The construct inserted in YieldGard MON810 maize, produced by Monsanto, contains the CaMV 35S promoter, the hsp70 intron of maize, the cryI(A)b gene for resistance to lepidopterans and the NOS terminator. In a previous work a truncation event at the 3' end of the cryI(A)b gene leading to the complete loss of the NOS terminator was demonstrated. The...
Article
In a preliminary study on the interaction between an Alternaria alternata pathotype and Solanum tuberosum a series of parameters, indicative of active and passive defence processes, was investigated in vitro using tolerant (Chiquita) and susceptible (Superior) cultivars. Ion leakage experiments along with growth of suspension cultures and plating e...
Article
Label-free and real-time DNA sequence detection in PCR-amplified DNA samples can now be achieved by different approaches. On the contrary, only few works have been reported dealing with direct sequence detection in nonamplified genomic DNA. Here, a piezoelectric biosensor for direct detection of sequences in nonamplified genomic DNA is described. T...
Chapter
The living state of matter, while obviously obeying physical and chemical laws, does exhibit some peculiar features which, taken altogether, distinguish it from the rest of the natural world. As discussed at length elsewhere [16, 18], these include, among others, hierarchical organization, individuality, invention, and homeostasis. One key category...
Article
We investigate on a possible way to connect the presence of low-complexity sequences (LCS) in DNA genomes and the non-stationary properties of base correlations. Under the hypothesis that these variations signal a change in the DNA function, we use a new technique, called non-stationarity entropic index (NSEI) method, and we prove that this techniq...
Article
The work evaluated a series of approaches to optimise detection of polymerase chain reaction (PCR) amplified DNA samples by an optical sensor based on surface plasmon resonance (SPR) (BiacoreX). The optimised procedure was based on an asymmetric PCR amplification system to amplify predominantly one DNA strand, containing the sequence complementary...
Article
A considerable amount of evidence has been obtained in recent years on the statistics of nucleotide distributions in DNA sequences. The present paper is ventured on the problem of the biological meaning of the fractal nature of DNA. The relative weight of a class of non random sequences (purine, pyrimidine, A-T, G-C. 1-3 nucleotide repeats) was ana...
Article
Full-text available
Previous work has shown the fixation of context-specific random amplified polymorphic DNA (RAPD) patterns in tomato cell cultures grown for 2 years in different hormonal contexts. In this work, RAPD sequences were characterised and RAPD-derived molecular markers used for a further study of variation between and within auto- and auxo-trophic tomato...
Article
A piezoelectric affinity sensor, based on DNA hybridisation has been studied for applications to Genetically Modified Organisms (GMOs) detection. The thiol/dextran modified surfaces were coupled to streptavidin for immobilising 5'-biotinyltead probes (25-mer). The probes sequences were respectively internal to the amplified product of P35S and T-NO...
Article
Full-text available
A new binary vector encoding for Candida albicans dihydrofolate reductase (DFR1) has been constructed and used as a dominant selectable marker for plant transformation. Transgenic tobacco plants with an increased resistance to methotrexate (Mtx) were obtained by co-transformation of tobacco leaf discs with Agrobacterium tumefaciens strains carrying...