Patrik Krieger

Patrik Krieger
Ruhr-Universität Bochum | RUB · Abteilung für Systemische Neurowissenschaften

PhD

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48
Publications
6,741
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1,205
Citations

Publications

Publications (48)
Article
Full-text available
Multisensory integration of auditory and tactile information occurs already at the level of the cochlear nucleus. Rodents use their whiskers for tactile perception to guide them in their exploration of the world. As nocturnal animals with relatively poor vision, audiotactile interactions are of great importance for this species. Here, the influence...
Article
Full-text available
Key points: Thalamic activity is regulated by corticothalamic feedback from layers 5B and 6. To selectively study the importance of the layer 6 corticothalamic (L6 CT) projection a transgenic mouse line was used in which layer 6 cells projecting to posterior medial thalamus (POm) were targeted for expression of channelrhodopsin-2. Repetitive optog...
Article
Full-text available
In the mouse whisker system, the contribution of L6 corticothalamic cells (L6 CT) to cortical and thalamic processing of the whisker deflection direction was investigated. A genetically defined population of L6 CT cells project to infragranular GABAergic interneurons that hyperpolarize neurons in somatosensory barrel cortex (BC). Optogenetic activa...
Article
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A fundamental task for the brain is to determine which aspects of the continuous flow of information is the most relevant in a given behavioral situation. The information flow is regulated via dynamic interactions between feedforward and feedback pathways. One such pathway is via corticothalamic feedback. Layer 6 (L6) corticothalamic (CT) cells mak...
Article
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The brain cytoplasmic (BC1) RNA is a non-coding RNA (ncRNA) involved in neuronal translational control. Absence of BC1 is associated with altered glutamatergic transmission and maladaptive behavior. Here, we show that pyramidal neurons in the barrel cortex of BC1 knock out (KO) mice display larger excitatory postsynaptic currents and increased spon...
Article
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The transmission of noxious stimuli from peripheral receptors to the cortex involves multiple central ascending pathways. While projections to areas in the brainstem and diencephalon are likely involved in mediating the immediate behavioural responses to pain, the assessment of the sensory and emotional/motivational components of pain are likely pr...
Article
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Layer 5 (L5) is a major neocortical output layer containing L5A slender-tufted (L5A-st) and L5B thick-tufted (L5B-tt) pyramidal neurons. These neuron types differ in their in vivo firing patterns, connectivity and dendritic morphology amongst other features, reflecting their specific functional role within the neocortical circuits. Here, we asked w...
Article
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Zusammenfassung Die Übersetzung von schmerzhaften Reizen in Schmerzempfindungen wird durch mehrere periphere und zentrale Signalwege des Nervensystems verwirklicht. Man nimmt heute an, dass die Organisation dieser Signalwege die beiden Hauptfunktionen der Schmerzwahrnehmung wiederspiegeln: die Bewertung von schmerzhaften Reizen (wo, was, wie stark)...
Article
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The transduction of painful stimuli into the experience of pain involves several peripheral and central signaling pathways of the nervous system. The organization of these pathways parallels the main functions of pain: the assessment of noxious stimuli (where, what, how strong), and the negative emotion of unpleasantness. Multiple lines of evidence...
Article
Fragile X syndrome (FXS) is a common inherited form of intellectual disability caused by the absence or reduction of the fragile X mental retardation protein (FMRP) encoded by the FMR1 gene. In humans, one symptom of FXS is hypersensitivity to sensory stimuli, including touch. We used a mouse model of FXS (Fmr1 KO) to study sensory processing of ta...
Article
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The true revolution in the age of digital neuroanatomy is the ability to extensively quantify anatomical structures and thus investigate structure-function relationships in great detail. Large-scale projects were recently launched with the aim of providing infrastructure for brain simulations. These projects will increase the need for a precise und...
Article
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This is the author accepted manuscript. The final version is available from Frontiers via http://dx.doi.org/
Book
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Sensorimotor integration, the dynamic process by which the sensory and motor systems communicate with each other, is crucial to humans’ and animals’ ability to explore and react to their environment. This book summarizes the main aspects of our current understanding of sensorimotor integration in 10 chapters written by leading scientists in this ac...
Chapter
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Sensation in animals and humans is often an active process that involves motion, e.g., moving fingers on a textured surface and eye movements. In this dynamic process, motion and sensation are strongly interdependent: internal motor information is needed to interpret external sensory signals, and sensory information is used to shape appropriate beh...
Article
Full-text available
A major synaptic input to the thalamus originates from neurons in cortical layer 6 (L6); however, the function of this cortico-thalamic pathway during sensory processing is not well understood. In the mouse whisker system, we found that optogenetic stimulation of L6 in vivo results in a mixture of hyperpolarization and depolarization in the thalami...
Article
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Morphological and functional classification of individual neurons is a crucial aspect of the characterization of neuronal networks. Systematic structural and functional analysis of individual neurons is now possible using transgenic mice with genetically defined neurons that can be visualized in vivo or in brain slice preparations. Genetically defi...
Article
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The striatum projection neurons are striatonigral and striatopallidal medium-sized spiny neurons (MSNs) that preferentially express D1 (D1R) and D2 (D2R) dopamine receptors, respectively. It is generally assumed that these neurons are physically intermingled, without cytoarchitectural organization although this has not been tested. To address this...
Article
Full-text available
The true revolution in the age of digital neuroanatomy is the ability to extensively quantify anatomical structures and thus investigate structure-function relationships in great detail. To facilitate the quantification of neuronal cell patterns we have developed RipleyGUI, a MATLAB-based software that can be used to detect patterns in the 3D distr...
Article
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Stimulation of sensory pathways is important for the normal development of cortical sensory areas, and impairments in the normal development can have long-lasting effect on animal's behavior. In particular, disturbances that occur early in development can cause permanent changes in brain structure and function. The behavioral effect of early sensor...
Article
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The aim of this paper is to apply a non-parametric statistical tool, Ripley's K-function, to analyze the 3-dimensional distribution of pyramidal neurons. Ripley's K-function is a widely used tool in spatial point pattern analysis. There are several approaches in 2D domains in which this function is executed and analyzed. Drawing consistent inferenc...
Article
In spines on basal dendrites of layer 2/3 pyramidal neurons in somatosensory barrel cortex, calcium transients evoked by back-propagating action potentials (bAPs) were investigated (i) along the length of the basal dendrite, (ii) with postnatal development and (iii) with sensory deprivation during postnatal development. Layer 2/3 pyramidal neurons...
Conference Paper
The distribution of neurons can be described as a spatial point pattern, a multi-dimensional stochastic process. The reorganization of neurons occurring in some brain diseases can many times be subtle, and thus improved statistical methods are necessary to adequately analyze the 3D distribution of neurons. A spatial point pattern is an extension of...
Article
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To understand sensory representation in cortex, it is crucial to identify its constituent cellular components based on cell-type-specific criteria. With the identification of cell types, an important question can be addressed: to what degree does the cellular properties of neurons depend on cortical location? We tested this question using pyramidal...
Conference Paper
In BAC (Bacterial artificial chromosome) transgenic mice we investigated the anatomical and functional properties of two populations of layer 5 pyramids in somatosensory and visual cortex. The two populations were selectively labelled by EGFP expressed under the control of cell-type specific promoters. The genotypic profile discriminated between th...
Article
Astrocytes play a key role for maintenance of brain water homeostasis, but little is known about mechanisms of short-term regulation of astrocyte water permeability. Here, we report that glutamate increases astrocyte water permeability and that the molecular target for this effect is the aquaporin-4 (AQP4) serine 111 residue, which is in a strategi...
Article
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We describe here a molecular genetic approach for imaging synaptic inhibition. The thy-1 promoter was used to express high levels of Clomeleon, a ratiometric fluorescent indicator for chloride ions, in discrete populations of neurons in the brains of transgenic mice. Clomeleon was functional after chronic expression and provided non-invasive readou...
Article
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Rodent somatosensory barrel cortex is organized both physiologically and anatomically in columns with a cross-sectional diameter of 100-400 microm. The underlying anatomical correlate of physiologically defined, much narrower minicolumns (20-60 microm in diameter) remains unclear. The minicolumn has been proposed to be a fundamental functional unit...
Article
Na,K-ATPase maintains not only ionic homeostasis, but also participates in a multiprotein complex mediating intracellular signalling. We show that ouabain, a specific ligand for Na,K-ATPase, evokes calcium oscillations in hippocampal astrocytes in primary cultures. Coimmunoprecipitation studies suggest that the mechanism underlying these calcium os...
Article
Spinal networks generate the motor pattern underlying locomotion. These are subject to modulatory systems that influence their operation and thereby result in a flexible network organization. In this review, we have summarized the mechanisms by which the different metabotropic glutamate receptor subtypes fine-tune the cellular and synaptic properti...
Article
The effect of L-AP4, a group III mGluR agonist, on sensory synaptic transmission in the lamprey spinal cord has been analyzed. Paired recordings were made between cutaneous mechanosensory neurons (dorsal cells) and postsynaptic spinobulbar giant interneurons. L-AP4 reduced the monosynaptic dorsal cell-evoked EPSP, but at concentrations higher (200-...
Article
Full-text available
Metabotropic glutamate receptors (mGluRs) act as modulators in the CNS of vertebrates, but their role in motor pattern generation in particular is primarily unknown. The intracellular signaling mechanisms of the group I mGluRs (mGluR1 and mGluR5), and their endogenous role in regulating locomotor pattern generation have been investigated in the spi...
Article
Full-text available
Experimental and computational techniques have been used to investigate the group I metabotropic glutamate receptor (mGluR)-mediated increase in the frequency of spinal cord network activity underlying locomotion in the lamprey. Group I mGluR activation potentiated the amplitude of NMDA-induced currents in identified motoneurons and crossed caudall...
Article
Metabotropic glutamate receptor (mGluR) activation modulates the lamprey spinal locomotor network. Potentiation of the NMDA receptor current is observed suggesting that one possible mechanism for mGluR to regulate locomotion is through such an interaction. The present study investigates this possibility. The behavior of NMDA induced oscillations ar...
Article
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The pharmacology of calcium channels involved in glutamatergic synaptic transmission from reticulospinal axons in the lamprey spinal cord was analyzed with specific agonists and antagonists of different high-voltage activated calcium channels. The N-type calcium channel blocker omega-conotoxin GVIA (omega-CgTx) induced a large decrease of the ampli...
Article
The effect of metabotropic glutamate receptor (mGluR) agonists and antagonists on the spinal cord network underlying locomotion in the lamprey has been analysed. The specific group I mGluR agonist (R,S)-3,5-dihydroxyphenylglycine (DHPG) and the broad-spectrum mGluR agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD) both increase...
Article
1. Different metabotropic glutamate receptors (mGluRs) can modulate synaptic transmission in different regions in the CNS, but their roles at individual synaptic connections have not been detailed. We used paired intracellular recordings from reticulospinal axons and their postsynaptic target neurons in the lamprey spinal cord to investigate the ef...
Article
The effects of the metabotropic glutamate receptor (mGluR) agonist ACPD ((1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid) on single neurones and on the network underlying locomotion in the lamprey have been analysed. ACPD induces a depolarization in lamprey spinal cord neurones, which is insensitive to tetrodotoxin (TTX) and ionotropic glutamate...

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