Patrick Sorgeloos

• PhD
• Professor Emeritus at Ghent University

The brine shrimp Artemia are tiny crustaceans that inhabit saltwater habitats such as salt pans, coastal lagoons, and salt lakes. Also known as extremophiles, they thrive in extreme conditions including desiccation and high salinity, and are used as model organisms in scientific research across multiple disciplines, particularly in studies involvin...

The potential functional role(s) of heat shock protein 70 (Hsp70) in the brine shrimp, Artemia franciscana, a crucial crustacean species for aquaculture and stress response studies, was investigated in this study. Though we have previously reported that Hsp70 knockdown may have little or no impact on Artemia development, the gestational survival an...

This review first presents the origin and species of wild discus fish (Symphysodon spp.), as well as discus propagation and domestication. The phenotypic characteristics, with particular emphasis on primary and secondary colors, stress bars, stripes and spots, eye features, the “D” area, markings and rims, body and fin shapes, forehead types, and g...

Pandanus tectorius leaf extract effect on the White-leg shrimp Penaeus vannamei tolerance against Vibrio parahaemolyticus were investigated in this study. Thirty shrimp post-larvae measured at approximately 1 cm were exposed for 24 h to 0.5, 1, 2, 3, 4, 5 and 6 g/L leaf extract and subsequently observed for survival and immune-related genes express...

The ornamental fish industry has continued to flourish since eighteenth century with increased fascination by enthusiasts in the striking body colours and patterns displayed in the fishes, a beneficial outcome of rigorous selective programmes. The expression of these pigmented colours is the result of the differentiation and orientation of speciali...

Artemia nauplii is an excellent live food source to fulfill the nutritional requirement in larviculture of crustaceans and marine fish due to its high digestible protein content, essential fatty acid and small in size (approximately 450 µm) (Sorgeloos et al., 1998, 2001). Artemia is mainly marketed worldwide as encapsulated cysts which allow it for...

The cover image is based on the Review Article Optimization of aquaculture sustainability through ecological intensification in China by Shuang‐lin Dong et al., https://doi.org/10.1111/raq.12648 The cover image is based on the Review Article Optimization of aquaculture sustainability through ecological intensification in China by Shuang‐lin Dong et...

The global demand for high-quality animal protein is expected to increase considerably with the rise in the human population. It is predicted that farmed crustacean shrimps could contribute significantly to this increasing demand, and eventually to the world´s future food protein security and nutrition. Diseases, however, impose a major yield-limit...

As the world's leading producer of farmed aquatic food products, China faces great uncertainty concerning the further sustainable development of its aquaculture industry due to high and increasing pressure from environmental and resource constraints. To realize truly sustainable development, it is imperative to establish an evaluation system that i...

Temperature, pH, salinity and ammonia stress adversely affect the performance and well-being of aquatic organisms, especially those used in aquaculture, a major food production sector. Management strategy to mitigate stress caused by abiotic factors in farmed aquatic organisms is scarce but is highly warranted considering the negative impact of the...

Oxidative stress caused by chemicals brings about mass mortalities in aquatic organisms and in this context, antioxidants and heat shock protein 70 (Hsp70) stimulating compounds represent potential alternatives to alleviate this problem during aquaculture. The antioxidant capacity and efficacies of five Malaysian indigenous microalgae, namely Auxen...

Females of the brine shrimp Artemia franciscana produce either free-swimming nauplii via ovoviviparous pathway of reproduction or encysted embryos, known as cysts, via oviparous pathway, in which biological processes are arrested. While previous study has shown a crucial role of ATP-dependent molecular chaperone, heat shock protein 70 (Hsp70) in pr...

The blue mussel Mytilus edulis is an intensely studied bivalve in biomonitoring programs worldwide. The lack of detailed descriptions of hemolymph-withdrawal protocols, particularly with regard to the place from where hemolymph could be perfused from, raises questions regarding the exact composition of aspirated hemolymph and does not exclude the p...

Brine shrimp Artemia franciscana release either encysted embryos via oviparous reproduction or motile nauplii via ovoviviparous reproduction. Artemia cysts contain an abundant amount of ArHsp21, ArHsp22, artemin, and p26, small heat shock proteins which regulate diapause and stress tolerance of this branchiopod crustacean. However, not much is know...

Though aquaculture plays an important role in providing foods and healthy diets, there are concerns regarding the environmental sustainability of prevailing practices. This study examines the trends and changes in fisheries originating from aquaculture production in Thailand and provides insights into such production’s environmental impacts and sus...

Acute Hepatopancreatic Necrosis Disease (AHPND), a disease caused by Vibrio parahaemolyticus (VpAHPND), kills Penaeid shrimps worldwide, resulting in severe economic losses during aquaculture. To further understand how Penaeus monodon post-larvae (PL) respond towards infection of this pathogenic bacterium, the expression of several important immune...

Aquatic ecosystems that form major biodiversity hotspots are critically threatened due to environmental and anthropogenic stressors. We believe that, in this genomic era, computational methods can be applied to promote aquatic biodiversity conservation by addressing questions related to the evolutionary history of aquatic organisms at the molecular...

The brine shrimp Artemia is a highly required, convenient and cost‐effective live food used in fish and shellfish larviculture. Its cysts, originating from a limited number of inland salt lakes, are traded worldwide. Over the past decades, Artemia pond production in solar saltworks, integrated with salt production, has emerged as an alternative to...

Vibrio species are a significant causative of mass mortality in mariculture worldwide, which can quickly accumulate in live food and transmit into the larval gut. With restrictions on the use of antibiotics in aquaculture, finding a proper solution to reduce the risk of Vibriosis is vital. This study aimed to evaluate the susceptibility of Vibrio h...

Crustacea, the subphylum of Arthropoda which dominates the aquatic environment, is of major importance in ecology and fisheries. Here we report the genome sequence of the Pacific white shrimp Litopenaeus vannamei, covering ~1.66 Gb (scaffold N50 605.56 Kb) with 25,596 protein-coding genes and a high proportion of simple sequence repeats (>23.93%)....

Marine fish larvae and crustaceans need, during the first periods of larval life, to be fed with zooplankton reared with the use of microalgae (phytoplankton) and enriched with omega-3 fatty acids using specialized products. On the contrary freshwater fish can be fed directly with artificial feeds. Furthermore, one of the most difficult periods of...

Habitat salinity is a major abiotic factor governing the activity, physiology, biology and distribution of aquatic animals. Salinity changes cause salt stress, affecting crustaceans reared in aquaculture both on an ecological and economic level. Current salt stress research in aquatic animals is mainly focused on salt stress in the gills at relativ...

The availability of high-quality juveniles is a bottleneck in the farming of many marine fish species. Detrimental larvae-microbe interactions are a main reason for poor viability and quality in larval rearing. In this review, we explore the microbial community of fish larvae from an ecological and eco-physiological perspective, with the aim to dev...

Seasonal Artemia pond production has been practiced for several decades by rural salt producing communities in the Mekong Delta, Vietnam, with more success than elsewhere in the world. In spite of this success, yields have been stagnating for a long time due to suboptimal feeding conditions. As feeding is one of the most important factors affecting...

This work brings together the view of different specialists in the areas of larviculture, physiology, ecology, nutrition and animal health, regarding how to deal with the aquaculture farming of species with complex life cycles in a multidisciplinary way, using as models the octopus and the muricid C. concholepas, with the aim of reducing the gap be...

The aim of this study was to characterize histological changes during opercular osteogenesis in farmed gilthead sea bream Sparus aurata larvae from 7 to 69 days post hatching (dph) and compare normal osteogenesis with that of deformed opercles. Mild opercular deformities were first detected in 19 dph larvae by folding of the opercle's distal edge i...

The blue mussel (Mytilus edulis) is known as a robust mollusk species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated 17 strains from induced mortality events in healthy wild-caught blue mussel adults and demonstrated that they caused between 17 and 98% mortality in blue mussel larvae in a newly d...

Acute hepatopancreatic necrosis disease (AHPND) is a severe shrimp disease that causes significant losses in the shrimp industry worldwide. In 2013, specific strains of Vibrio parahaemolyticus were found to be responsible for AHPND. Recently, inhibiting the detection of catecholamines has been reported to decrease the virulence of various pathogeni...

The sex of relatively primitive animals such as invertebrates is mostly determined by environmental factors and chromosome ploidy. Heteromorphic chromosomes may also play an important role, as in the ZW system in lepidopterans. However, the mechanisms of these various sex determination systems are still largely undefined. In the present study, a Ma...

In arthropods, mature females under certain conditions produce and release encysted gastrula embryos that enter diapause, a state of obligate dormancy. The process is presumably regulated by diapause hormone (DH) and diapause hormone receptor (DHR) that were identified in the silkworm, Bombyx mori and other insects. However, the molecular structure...

Intensive Pangasius aquaculture in Vietnam has attracted concerns about the sustainability of its resource use. However, the assessment of the latter, using Life Cycle Assessment (LCA), is a time-consuming and complex task. To establish a simplified approach, especially relevant for certification incentives, as an alternative to a full LCA, we firs...

As earlier observations on peroral infectivity of WSSV in white-legged shrimp are conflicting, here, a standardized peroral intubation technique was used to examine (i) the role of the physical composition of the viral inoculum and (ii) the barrier function of the PM. In a first experiment, the infectivity of a WSSV stock was compared by determinin...

Innovation is essential in the modern world in order to survive or to be successful. There are ways to promote innovation and provide support for enterprises with innovation approach. One supportive platform in Europe is the European Aquaculture Technology and Innovation Platform (EATiP). The main role of these platforms is to define long term visi...

The beneficial effects of PHB as supplement for giant tiger prawn Penaeus monodon postlarvae using a short-term enrichment strategy via Artemia were examined. The effects of co-supplementing with a lipid emulsion were also evaluated to determine whether it yielded an additional benefit. Results on the average weight and larval development were not...

In the field, moulting and salinity drop in the water due to excessive rainfall have been mentioned to be risk factors for WSSV outbreaks. Therefore, in this study, the effect of an acute change in environmental salinity and shedding of the old cuticle shell on the susceptibility of Penaeus vannamei to WSSV was evaluated by immersion challenge. For...

The brine shrimp Artemia franciscana is an important live feed for fish and shellfish larviculture. Cysts of Artemia are naturally found in a wide variety of harsh environments worldwide where they are exposed to different changing environmental conditions. Artemia cysts are also exposed to different hydration/dehydration (H/D) conditions during th...

This study was conducted to test the effect of DnaK on priming immune responses in Penaeid shrimp. Juvenile-specific pathogen-free (SPF) P. vannamei shrimp were injected with 0.05 μg recombinant DnaK. One hour post-DnaK priming, a non-lethal dose of Vibrio campbellii (10(5 ) CFU shrimp(-1) ) was injected. Other treatments include only DnaK or V. ca...

Public support of mussel farming in Greece is an important factor because of its financial viability. The profitability of the activity is seriously reduced in small farms (1–3 ha) as a result of their high production costs; however, small farms represent a major part of the industry. Mussel farming is an extensive activity, with space availability...

Nauplii hatching from Artemia cysts are crucial in larviculture nutrition. Artemia cysts may be exposed to repeated hydration/dehydration (H/D) cycles pre-harvesting or during processing and storage. To observe the effect of these cycles on cyst quality, Artemia franciscana cysts were exposed to a comprehensive set of various H/D treatments, differ...

The long-running higher education network AQUA-TNET, a tightly-knit collaboration of university departments, research institutes and other stakeholders from the aquaculture industry, was established in 1996 by AQUATT, a coordinating partner in the SOCRATES-ERASMUS Thematic Network DEMETER led by ICA (Association for European Life Sciences Universit...

The notion that phenotypic traits emerging from environmental experiences are heritable remains under debate. However, the recent report of nonmendelian transgenerational epigenetic inheritance, i.e., the inheritance of traits not determined by the DNA sequence, might make such a phenomenon plausible. In our study, by carrying out common garden exp...

and impoverished microbial community (and a consequent lack of competition) favors fast-growing bacteria (such as many pathogenicVibriospp.) in recolonizing the environment [4]. Considering that EMS/ AHPND most probably is caused by a Vibrio, this practice is thus more likely to stimulate proliferation of the EMS/ AHPND-causing agent in the pond th...

The beneficial effects of poly-β-hydroxybutyrate (PHB) for aquaculture animals have been shown in several studies. The strategy of applying PHB contained in a bacterial carrier has, however, hardly been considered. The effect of administering PHB-accumulated Alcaligenes eutrophus H16 containing 10 or 80 % PHB on dry weight, named A10 and A80, respe...

An alternative larval shrimp feeding regime, in which umbrella-stage Artemia were constituting the first zooplankton source was evaluated in the culture of Litopenaeus vannamei. In a preliminary experiment, umbrella-stage Artemia were fed to larvae from zoea 2 (Z2) to mysis 2 (M2) stages to identify the larval stage at which raptorial feeding start...

Aquaculture has expanded from an insignificant contribution of less than 10% in the quantity of aquatic products for human consumption in the 1970s to about 50% at present Furthermore, expectations are high for aquaculture to fill further needs in the future as capture fisheries are stagnating and human needs are increasing. This article reviews th...

We studied the effect of pH (pH 5, 6, 7 and 8) on the hatching percentage, survival and reproduction of Artemia strains in Bohai Bay salt ponds. Strains included parthenogenetic Artemia from Bohai Bay (BHB), Artemia franciscana from San Francisco Bay, and A. franciscana artificially produced in salt ponds in Vietnam. The latter was included as a po...

This chapter provides an update on the most common zooplankton live feed species used in hatchery rearing of fish and shellfish larvae, namely rotifers, Artemia and copepods. Each section starts with a summary of the biology and ecology of these species. An overview of the most common techniques to culture, feed, harvest, disinfect and preserve the...

This paper studied the effects of increased carbon (C) and nitrogen (N) supplementation in pond production of Artemia franciscana cysts in southern Vietnam. Pig manure and tapioca were used to supplement the natural food of Artemia in the ponds at a fixed C/N ratio 10. Addition of pig manure and tapioca at a standard rate (0.210 kg ww and 0.319 kg...

Abstract

Pathogenic bacteria communicate with small signal molecules in a process called quorum sensing, and they often use different signal molecules to regulate virulence gene expression. Vibrio campbellii, one of the major pathogens of aquatic organisms, regulates virulence gene expression by a three channel quorum sensing system. Here we show that altho...

The effects of feeding Artemia nauplii enriched with or without poly-b-hydroxybutyrate (PHB) and/or highly unsaturated fatty acids (HUFA) on Siberian sturgeon (Acipenser baerii) larvae were investigated. Feeding larvae with PHB-enriched nauplii (PHB, PHB + HUFA) decreased the growth performance of the larvae. PHB affected the body composition by in...

Depletion of marine fish stocks has become a global problem. Aquaculture is seen by many as the best way to meet the growing demand for fish products. The question then arises whether aquaculture is a truly environmentally sustainable alternative. Every type of aquaculture is different, therefore each one merits its own case study. To date, Life Cy...

Two groups of Anguilla japonica were treated with hormone, one on a weekly basis, the other on a biweekly basis. Intramuscular injection was applied at a dose of 0.75 mg/kg BW carp pituitary extract (CPE) plus 150 IU/kg BW human chorionic gonadotropin (hCG) for female fish, while males received half of this dose. The average total lipid content of...

The authors regret an error within the Abstract of their published paper. As published: Reverse transcriptase real-time PCR with specific primers revealed that the expression level of three phospholipase genes was 2-fold lower in the mutant with minimal quorum sensing activity than in the mutant with maximal quorum sensing activity. Should read: Re...

Knowledge on the importance of associations between bacteria and microalgae in aquatic ecosystems is rather limited at the moment, mostly due to a lack of studies at the molecular and biochemical level of microorganisms. This paper discusses the current knowledge on microalgae–bacteria interactions and their potential impacts on the productivity, e...

We report on the construction of sex-specific linkage maps, the identification of sex-linked markers and the genome size estimation for the brine shrimp . Overall, from the analysis of 433 AFLP markers segregating in a 112 full-sib family we identified 21 male and 22 female linkage groups (2 = 42), covering 1,041 and 1,313 cM respectively. Fifteen...

List of the 65 primer combinations used for AFLP analysis1 E: Eco RI primer with three selective bases; M: Mse I primer with three selective bases (1, 2, 3, 4 correspond to A, C, G, T). (DOC)

Up to now, only a few brief procedures for purifying white spot syndrome virus (WSSV) have been described. They were mainly based on sucrose, NaBr and CsCl density gradient centrifugation. This work describes for the first time the purification of WSSV through iodixanol density gradients, using virus isolated from infected tissues and haemolymph of...

The effect of carbon/nitrogen (C/N) ratio manipulation in feed supplements on Artemia production and water quality was investigated in solar salt ponds in the Mekong Delta, Vietnam. It was assumed that development of bacterial bioflocs through C/N manipulation would improve Artemia production and water quality as demonstrated in freshwater and mari...