Patrick Jame

French National Centre for Scientific Research | CNRS · Institute of Analytical Science (ISA)

Tonka bean extract, prepared from Tonka beans, is mainly composed of Coumarin. However, due to alternative industrial procedure of production, different solvents and mixtures of solvents were tested for extraction. Investigation using HPLC-DAD-MS indicates influence of extraction procedure on compositions of main constituents. New polyphenols struc...

The involvement of meteoric water in orogens dynamics through surface processes is well known as for example in the Himalayas where erosion, resulting of the interplay between climate and tectonics shapes the most spectacular landscapes on the planet. But what about more internal and deepest surface fluid infiltration? Here we report analysis of th...

A phyloprofile of Frankia genomes was carried out to identify those genes present in symbiotic strains of clusters 1, 1c, 2 and 3 and absent in non-infective strains of cluster 4. At a threshold of 50% AA identity, 108 genes were retrieved. Among these were known symbiosis-associated genes such as nif (nitrogenase), and genes which are not know as...

Geraniol derived from essential oils of various plant species is widely used in the cosmetic and perfume industries. It is also an essential trait of the pleasant smell of rose flowers. In contrast to other monoterpenes which are produced in plastids via the methyl erythritol phosphate pathway, geraniol biosynthesis in roses relies on cytosolic NUD...

This study focuses on the valorization of biomass waste from the fragrance industry in Provence, France. Adsorbents enriched with oxygen surface groups were produced from Lavandin (Lavandula genus) using the hydrothermal carbonization (HTC) process followed by further NaOH surface treatment. The original Lavandin hydrochar (LH) and NaOH surface tre...

The aim of this work was to investigate the use of biorefinery industry by-products such as humins as adsorbents for methylene blue (MB). After thermal treatment, these by-products are called biochars. Three biochars were used as adsorbents for MB removal: (i) a biochar obtained after thermal treatment of humins (called raw biochar), (ii) a biochar...

Saffron is a very high value-added ingredient used in the food supplement market and contains a high level of safranal. Adding synthetic safranal to saffron, which is significantly cheaper, and falsifying the origin of saffron may represent recurrent fraud. Saffron from different countries was analyzed to determine the stable isotope ratios δ13C an...

Natural plant extracts are primarily used as raw materials in the cosmetic and perfumery industry. However, adulterations with petrochemical products are occurring in the market, leading to non-100% natural products. Several analytical techniques such as impurity detection or enantioselective ratio assessments have been previously described as good...

Hydrochars obtained by hydrothermal carbonization (HTC) of Lavandula straws have been studied as an eco-friendly and economical valorization route of residual biomass from cosmetic industry into solid fuels. HTC process has been performed in a temperature range from 180 to 260 °C and retention time ranging from 1 to 20 h. The two key parameters (ti...

Bulk (Mo2N) and supported molybdenum nitride (Mo2N/TiO2, Mo2N/CeO2) were synthesized by reduction-nitridation of bulk and supported MoO3 under a mixture of N2/H2. We investigated the mechanism of formation of molybdenum nitrides by coupling elemental analysis, in situ and ex situ XRD, Raman spectroscopy, in situ XPS/UPS spectroscopy and DFT calcula...

Lavandin hydrochars obtained by hydrothermal carbonization (HTC) of Lavandula distilled straw (LDS) have been studied as an eco-friendly and economical valorization route of residual biomass from cosmetic industry into solid fuels. The critical parameters of the HTC process (temperature and retention time) were normalized to severity factor (SF). P...

Three different types of biomass sourced from forestry waste (eucalyptus residues), agricultural waste (wheat straw), and energy crop (miscanthus) were used as starting materials to produce hemicellulosic sugars, furans (furfural and hydroxymethylfurfural), and oligosaccharides. A two-step hybrid process was implemented; biomass was first autohydro...

Neroli essential oil (EO), extracted from bitter orange blossoms, is one of the most expensive natural products on the market due to its poor yield and its use in fragrance compositions, such as cologne. Multiple adulterations of neroli EO are found on the market, and several authentication strategies, such as enantioselective gas chromatography (G...

Parts made by metal additive manufacturing processes assisted by sintering suffer from embrittlement due to binder/powders interactions. Using a core-shell approach, we modelled the diffusion of carbon and oxygen from the binder into the metallic powders. The model was assessed by measuring the interstitials content upon various debinding/sintering...

Pure and natural essential oils of Allium species are primarily used in the food industry, but due to their low yield, production quantities can be difficult to achieve and thus can be highly expensive. Due to these problems, adulterations are a reality in this field. The purpose of this research is to develop a multi element stable isotope analysi...

Background: This study aims to describe and test a tiered approach for assessing compliance to Environmental Quality standards (EQSs) for priority substances in biota in line with the European Water Framework Directive. This approach is based on caged gammarids and trophic magnification factors (TMFs) at the first tier, with fish analysed at the se...

Stable isotope compositions of ancient halite fluid inclusions have been recognized to be valuable tools for reconstructing past environments. Nevertheless, in order to better understand the genesis of halite deposits, it could be of great interest to combine both δ2H and δ18O measurements of the water trapped as inclusions in the defects of the mi...

A reduction in CO2 emissions is required to mitigate global warming. Post-combustion carbon capture is one of the most developed technologies that has the potential to meet this goal, but its cost prevents its widespread use. A different approach would be to use CO2 directly as it is captured, before it is stored. Here we explore spontaneous CO2 fi...

The essential oil of wintergreen, which is extracted by steam distillation from Gaultheria genus leaves, is mainly used in aromatherapy. However, due to its adulteration easiness with synthetic material, it is necessary to control samples naturalness with accuracy. The purpose of this work was to develop a methodology to authenticate the essential...

The process of carbon capture, which is one of the most mature yet cost-intensive technology proposed to mitigate global warming has herein been explored as a potential strategy to generate dynamic ligands for metal separation and recovery. Spontaneous CO2 fixation by industrial amines such as diethylenetriamine affords dynamic arrays of interconve...

One of the most adopted solutions in developed countries to manage stormwater is detention/retention basins which generate large quantities of sediments that have to be removed regularly. In order to manage them properly, accurate data are needed about their physical and chemical characteristics, particularly on micropollutant concentrations and th...

The design of French VFCWs leads to the formation of a sludge layer at the surface of the first filters due to the retention of suspended solids from the percolation of unsettled wastewater. This layer plays a major role in the system but still little is known on its characteristics and evolutions. In this study, suspended solids and sludge deposit...

Are bioelectrochemical systems suitable to fight against the global warming provoked by the anthropogenic sources of CH4? None of the existing studies on this topic provide clear answers because the performance criteria of the electrochemical treatment and gas quantification methods used so far are questionable. This article redefines this performa...

The economic market represented by the natural products has been in very strong expansion for these last years, pushed by the craze of the consumers for this type of label. The very strong price difference with the equivalent flavor from pure synthesis results in a phenomenon of fraud by a non-compliance between the labeling and the contents of the...

Phenacetin is a pharmaceutical closely related to acetaminophen that has been banned in France for a long time due to its nephritic and carcinogenic adverse effects. It frequently appears in cocaine seizures as a cutting agent. Following both sanitary and intelligence motivations, this molecule was chosen for this study, and stable isotopes seemed...

Open-Bio was a research project funded by the European Commission within the 7th Framework Programme for Research and Technological Development FP7. The goal was to investigate how bio-based products can be integrated into the market using standardisation, labelling and procurement. One part of the project (WP5: In-situ biodegradation) was focussed...

RationaleTo develop more eco-friendly laundry detergents, renewable surfactants synthesized from vegetal sources are increasingly being used. In a more stringent regulation context, the determination of bio-sourced surfactant origin thus appears essential to assess the claims of detergent manufacturers. Radiocarbon determination, the standard metho...

The development of a method for the determination of the oxygen amount in liquid fuels is described using a new device made by a vertical pyrolysis unit linked to a non dispersive CO infrared detector. The out coming signal is amplified using an additional electronic conversion card monitored by homemade software. This system allows the quantificat...

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Acknowledgements The authors would like to thank Hervé Casabianca, Vanessa Salomon and Patrick Goetinck from CNRS for their involvement in isotopic ratio mass spectrometry and Richard Watts from CRM International for his inputs to this project, providing standards and technical assistance.

Soils contaminated by prions, the infectious agents responsible for transmissible spongiform encephalopathy diseases, remain infectious to grazing animals for many years. In this study, the ability of enzymes produced by soil microbes to degrade a recombinant prion protein (recPrP) was investigated in a loamy soil. A 15N‐labelled recPrP was added t...

Jame P., Casabianca H., Batteau M., Goetinck P., Guibert S.

δ13C values of pollen grains belonging to different plant species (trees, herbaceous no poaceae, poaceae) were measured. Most of these temperate species are C3 plants with δ13C values between −28.6 ‰ and −21.7 ‰, the C4 plants have more positive values between −15.9 ‰ and −10 ‰. These results corroborate the interest of such measurements to differe...

δ13C values of pollen grains belonging to different plant species (trees, herbaceous no poaceae, poaceae) were measured. Most of these temperate species are C3 plants with δ13C values between -28.6 ‰ and -21.7 ‰, the C4 plants have more positive values between -15.9 ‰ and -10 ‰. These results corroborate the interest of such measurements to differe...

δ13C values of pollen grains belonging to different plant species (trees, herbaceous no poaceae, poaceae) were measured. Most of these temperate species are C3 plants with δ13C values between -28.6 ‰ and -21.7 ‰, the C4 plants have more positive values between -15.9 ‰ and -10 ‰. These results corroborate the interest of such measurements to differe...

This work presents two techniques for testing for the adulteration of flavors. One uses multidimensional gas chromatography on cyclodextrin derivatives to separate chiral compounds, the other uses isotope ratio mass spectrometry coupled with gas chromatography to determine stable isotope ratios. Applications to fruits and plants for the food and pe...

An intensive multidisciplinary experiment has been conducted over several years at La Côte Saint-André, near Grenoble, France. The major objective is to determine an optimal fertilizer application scheme for an irrigated agricultural system. Such a scheme would not degrade the quality of the environment, and yet would maintain a profitable level of...

Stable carbon isotope analysis of various heavy oils and some thermo-catalytically converted products was performed with a thermal analyser coupled with an isotopic ratio mass spectrometer. The temperature-programmed oxidative pyroanalysis technique subdivides the classical ratio, affording new insights into the structural composition of heavy oils...