Patrick Brice Deeh Defo

• PhD
• Research Associate at Kangwon National University

Helichrysum odoratissimum is commonly used in the treatment of diabetes, skin wounds, and eczema. We determined its phytochemical composition and evaluated its antioxidant, anti-tyrosinase, antidiabetic, wound healing, and cytoprotective activities. The antioxidant effect was tested by DPPH, ABTS+, cupric, and ferric methods. The anti-tyrosinase ac...

Cyclophosphamide (CP) chemotherapy is commonly associated with various side effects. The development of an effective therapy capable of counteracting these effects is of great interest. We evaluated the effects of a novel polyherbal formulation (PHF) on CP cytotoxicity in TM3 cells and fictive ejaculation in rats and determined its possible mechani...

Endophytic fungi, which reside in medicinal plants, are considered potential sources of therapeutic production by mimicking the host plant metabolisms. Additionally, they have the capability to produce novel metabolites through complex gene expression. Endophytes, their crude extracts, or active molecules derived from them are employed for plant pr...

We fabricated silver nanoparticles (AgNPs) using Pterorhachis zenkeri, characterized by Atomic Force Microscopy (AFM) and determined their antioxidant potentials in vitro. Results confirmed the fabrication of AgNPs by using P. zenkeri as a bioreducing agent for the first time. AgNPs possessed potent antioxidant activity in vitro. Furthermore, the T...

We fabricated silver nanoparticles (AgNPs) using Pterorhachis zenkeri , characterized by Atomic Force Microscopy (AFM) and determined their antioxidant potentials in vitro . Results confirmed the fabrication of AgNPs by using P. zenkeri as a bioreducing agent for the first time. AgNPs possessed potent antioxidant activity in vitro. Furthermore, the...

This study was undertaken to determine the phytochemical profile, antioxidant and cytotoxicity of the aqueous extracts of Dracaena arborea (DA) and Bridelia ferruginea (BF). The phytochemical composition, total phenolic (TP) and flavonoid (TF) contents of the extracts were determined by GC-MS, Folin Ciocalteu and AlCl 3 methods, respectively. The a...

Background: Cyclophosphamide (CP) is an anticancer agent, but its chronic administration induces ovarian toxicity. Objective: We evaluated the effects of aqueous extract (AE) and methanol extract (ME) of Amaranthus hybridus (A. hybridus) on CP-induced ovarian toxicity in rats. Materials and methods: 40 female Wistar rats (10 wk, 170-200 gr) we...

Osteoarthritis (OA) is a musculoskeletal disorder mainly found in elderly individuals. Modern treatment of OA, like nonsteroidal anti-inflammatory drugs, corticosteroids, hyaluronic acid injections, etc., is linked to long-term side effects. We evaluated the anti-osteoarthritic properties of a novel joint health formula (JHF) containing Bisdemethox...

Magnesium biotinate (MgB) is a novel biotin complex with superior absorption and anti-inflammatory effects in the brain than D-Biotin. This study aimed to investigate the impact of different doses of MgB on social behavior deficits, learning and memory alteration, and inflammatory markers in propionic acid (PPA)-exposed rats. In this case, 35 Wista...

Phyllanthus muellerianus (PM) and Ficus exasperata (FE) are plants used against cancers. We evaluated the phytochemical profiles and in vitro antioxidant potentials of PM and FE, and investigate their effects on cell proliferation, intracellular calcium ([Ca2+]i), caspases 3/9, apoptosis, oxidative stress markers, and Bax/cytochrome C expression in...

This study was conducted to compare the effects of a novel form of magnesium, Mg picolinate (MgPic), to magnesium oxide (MgO) on metabolic and cognitive functions and the expression of genes associated with these functions in rats fed a high-fat diet (HFD). Forty-two Wistar rats were divided into six groups: control, MgO, MgPic, HFD, HFD + MgO, and...

Purpose Phyllanthus muellerianus (PM) and Ficus exasperata (FE) are plants used against cancers. We evaluated the phytochemical profiles and in vitro antioxidant potentials of PM and FE, and investigate their effects on cell proliferation, intracellular calcium ([Ca²⁺]i), caspases 3/9, apoptosis, oxidative stress markers, and Bax/cytochrome C expre...

Magnesium (Mg) is an essential mineral required for many physiological processes, including ionic balances in ocular tissues. We compared the effects of different Mg-chelates (Mg oxide, MgO vs. Mg picolinate, MgPic) on retinal function in a high-fat diet (HFD) rats. Forty-two rats were divided into six groups and treated orally for 8 weeks as follo...

Varicocele is a disease characterized by an abnormal dilation of the pampiniform plexus that drains the testis. The main objective of this work was to evaluate the curative effects of aqueous and ethanolic extracts of Dracaena arborea on some reproductive and antioxidant markers in rats with experimental varicocele. Following varicocele induction,...

Objective: We evaluated the preventive effects of aqueous and ethanolic extracts of Dracaena arborea on sperm characteristics and oxidative stress markers in adult male rats with varicocele. Methods: Thirty-six male Wistar rats were randomly distributed into 6 groups (6 animals/group) and treated for 30 days as follows: (1), normal rats receivin...

Background/aim: Numerous studies show that cancer risk is reduced by consumption of soy-based foods containing genistein, but its effects on the glycogen synthase kinase-3 pathway (GSK-3) in ovarian cancer is unknown. Therefore, we tested the properties of genistein on inflammatory biomarkers and GSK-3 signaling pathways in the ovaries of old layi...

Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to facilitate delivery. This study was aimed at evaluating the in vitro uterotonic effects of aqueous and methanol extracts of L. acida in Wistar rats. Uterine strips isolated from rats pretreated with 5 μg estradiol (48 h)...

Background: Cryptorchidism (CPT) is an important cause of male infertility. Rubus apetalus is a medicinal plant with a powerful antioxidant potential. We investigated the effects of aqueous and methanolic extracts of R. apetalus on spermatozoa parameters, antioxidant enzymes and fertility potential of rats with experimental unilateral CPT. Method...

In December 2019, a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) that caused severe disease clusters was first reported in Wuhan, the capital of China's Hubei province. This viral disease, which is reported to originate from a seafood market where wild animals are illegally sold, has been transmitted among humans worldwide through c...

This study aimed to investigate the properties of Salacia chinensis (Celastraceae, SC) and its molecular mechanism in the type 2 diabetic rats. Forty‐two Wistar rats were divided into six groups (n = 7): control, SC (100 mg/kg, per os), high‐fat diet (HFD), HFD + SC (100 mg/kg), HFD + streptozotocin (STZ, 40 mg/kg, i.p.), and HFD + STZ+SC. SC decre...

The aim of this study was to evaluate the effects of Pterorhachis zenkeri (Meliaceae) on sex organ growth in immature male rats and, oxidative stress and apoptosis markers in CCL‐97 (R2C) Leydig cells. For the in vivo studies, 70 immature male Wistar rats (n = 10/group) were treated for 2 or 4 weeks with: distilled water (10 ml/kg, per os) plus soy...

Background: Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to solve difficult childbirth. This study aimed to evaluate the in vitro uterotonic effects of aqueous and methanolic extracts of L. acida in female Wistar rats. Uterine strips isolated from female rats pretreat...

Reproductive dysfunction is one of the most prevalent diabetes complications. Draceana arborea is known to enhance sexual function in diabetic rats, but the underlying mechanisms have not been thoroughly elucidated. This study examined the effects of D. arborea on some reproductive complications of diabetes in rats. Aqueous and ethanol (500 and 100...

Background: Raphia vinifera (Arecaceae) is a medicinal plant commonly used as a sexual enhancer. Objective: To investigate the aphrodisiac potential of aqueous extract (AE) and methanolic extract (ME) of R. vinifera in sexually experienced male rats. Materials and Methods: Thirty male Wistar rats were randomly distributed into six groups (5 rats pe...

ABSTRACT Objective: Cyclophosphamide (CP) is commonly used to treat some cancers, but its clinical efficacy is also linked with testicular toxicity. We investigated the effects of aqueous extract (AE) and methanolic extract (ME) of Helichrysum odoratissimum for reducing CP-induced reproductive toxicity in male rats. Methods: In addition to a normal...

Background We investigated the effects of a polyherbal formulation prepared from the extracts of Mondia whitei (Periplocaceae), Dracaena arborea (Dracaenaceae), and Bridelia ferruginea (Euphorbiaceae) (MDB) on the sexual behavior of normal rats (NR) and prediabetic rats (PR). Methods Male Wistar rats were administered with drinking fructose soluti...

The present study was undertaken to investigate the effect of the combination of soy protein, amylopectin, and chromium (SAC) on muscle protein synthesis and signal transduction pathways involved in protein synthesis (mTOR pathways, IGF-1, and AktSer473) and proteolysis (FOXO1Ser256; MURF1, MAFbx) after exercise. Thirty-five Wistar rats were random...

In the present study, we investigated the effects of chromium-picolinate (CrPic) and chromium-histidinate (CrHis) on nutrient digestibility and nutrient transporters in laying hens exposed to heat stress (HS). Hens (n = 1800; 16 weeks old) were kept in cages in temperature-controlled rooms at either 22 ± 2 °C for 24 h/day (thermoneutral (TN)) or 34...

Polycystic ovarian syndrome (PCOS) is one of the common causes or female infertility. Phyllanthus muellerianus (Euphorbiaceae) is a plant used to treat various ailments including frequent menstruation and anovulation. We investigated the effects of P. muellerianus extracts on estrus cyclicity, lipid profile, oxidative stress-related markers, sex ho...

Recent studies have demonstrated that hypothyroidism is associated with infertility. This work was undertaken to evaluate the protective effects of Aframomum melegueta on testicular functions and fertility of hypothyroid male rats. Male rats were orally treated with propylthiouracil (PTU: 10 mg/kg) in combination with plant aqueous or methanol seed...

Objective: Hypothyroidism has been claimed to generate sexual dysfunctions such as ejaculatory disorders. Aframomum melegueta is an aphrodisiac plant with pro-ejaculatory properties. We investigated the protective effects of aqueous extract (AE) and methanolic extract (ME) of A. melegueta on the ejaculatory function of hypothyroid male rats. Method...

Background: Guibourtia tessmannii (GT) (Caesalpiniaceae) is claimed as a plant having aphrodisiac property as per the African traditional medicine. Objective: This study evaluated the effects of the methanolic extract of GT on sexual behavior and fictive ejaculation in high-fat diet (HFD)-induced sexually sluggish male rats. Materials and Methods:...

Background Osteoporosis represents the most common metabolic bone disease. Bambusa vulgaris (Poaceae) is a plant with potential antiosteoporotic effects, due to its phytoestrogenic, antioxidative, and anti-inflammatory properties. This study was undertaken to evaluate the effects of aqueous and methanol extracts of B. vulgaris on osteoporosis in ra...

Allyl isothiocyanate (AITC), a dietary phytochemical found in some cruciferous vegetables, is commonly used as an antimicrobial, anticancer, and antioxidant agent. In the present study, we investigated the effects of AITC on insulin resistance and transcription factors in a diabetic rat model. A total of 42 Wistar rats were divided into six groups...

This study evaluated the effects of amylopectin (A) and chromium (Cr) forms alone or in combination on energy storage, muscle fatigue-related biochemical markers, and expression of peroxisome proliferator-activated receptors (PPAR-γ) and glucose transporter-2 and -4 (GLUT-2 and GLUT-4) in rats during exhaustive exercise. Thirty-five male Wistar rat...

This study evaluated the effects of the methanolic extract of Guibourtia tessmannii (GT) and selenium (Se) on cell viability, intracellular calcium concentration ([Ca²⁺]i), apoptosis and oxidative stress through transient receptor potential vanilloid 1 (TRPV1) channel activity in CCL‐97 (R2C) tumour Leydig cells. The cells were divided into nine gr...

Abstract Background Chromium histidinate (CrHis) and biotin are micronutrients commonly used to improve health by athletes and control glycaemia by patients with diabetes. This study investigates the effects of 8-week regular exercise training in rats together with dietary CrHis and biotin supplementation on glucose, lipids and transaminases levels...

Objective: To evaluate the effects of the methanolic extract of Guibourtia tessmannii (G. tesmannii) on sperm parameters, lipid profile and testosterone level in obese rats. Methods: A total of 193 male Wistar rats were fed either with palm oil diet (n=185) or standard diet (n=8) for 16 wk. At the end of this feeding period, 90 obese rats were sele...

Introduction: Obesity is rapidly becoming a worldwide problem affecting the reproductive system. Some studies have shown the relationship between obesity and infertility, but until now it remains controversial. The present study was undertaken to investigate the effects of a specific high-fat diet on the male rat reproductive performances.

Background Androgen deficiency is a clinical syndrome resulting from the inability of the testes to produce physiological levels of testosterone due to a disturbance occurring at one or more levels of the hypothalamic-pituitary-testicular axis. The present study was undertaken to evaluate the androgenic properties of aqueous and methanolic extracts...

Context: Guibourtia tessmannii (Caesalpiniaceae) is a plant traditionally used as aphrodisiac. We previously reported the pro-ejaculatory effects of the aqueous and methanol extracts of G. tesmannii in spinal male rat. However, the mechanism underlying such effects has not been elucidated. Objective: This study characterizes the dopaminergic sub-ty...

Background Mondia whitei and Guibourtia tessmannii are used in Cameroon traditional medicine as aphrodisiacs. The present study was undertaken to evaluate the pro-ejaculatory effects of the aqueous and organic solvent extracts of these plants in spinal male rats. Methods In spinal cord transected and urethane-anesthetized rats, two electrodes wher...