Patricia Taladriz

Patricia Taladriz
International Iberian Nanotechnology Laboratory

PhD in Chemistry

About

46
Publications
3,732
Reads
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583
Citations
Additional affiliations
May 2015 - April 2016
University of Cambridge
Position
  • Visiting Postdoctoral Researcher
Description
  • Real-time detection of nitric oxide in microdroplets via in situ SERS and SAXS spectroscopies
September 2013 - May 2015
University of Campinas
Position
  • PostDoc Position
Description
  • Nanoparticles and hydrogels for the photochemical delivery of nitric oxide
September 2010 - December 2010
University of Catania
Position
  • Visiting PhD Student
Description
  • Amplification of Nitric Oxide release using Layer-by-Layer technique
Education
September 2007 - November 2012
University of Vigo
Field of study
  • Nanobiomaterials
October 2001 - September 2007
University of Vigo
Field of study
  • Chemistry

Publications

Publications (46)
Article
Generation of amplified stimulated emission inside mammalian cells has paved the way for a novel bioimaging and cell sensing approach. Single cells carrying gain media (e.g., fluorescent molecules) are placed inside an optical cavity, allowing the production of intracellular laser emission upon sufficient optical pumping. Here, we investigate the p...
Article
Full-text available
The approval of new nanomedicines requires a deeper understanding of the interaction between cells and nanoparticles (NPs). Silica (SiO2) and gold (Au) NPs have shown great potential in biomedical applications, such as the delivery of therapeutic agents, diagnostics, and biosensors. NP-cell interaction and internalization can trigger several cellul...
Article
Plastic particle pollution has been shown to be almost completely ubiquitous within our surrounding environment. This ubiquity in combination with a variety of unique properties (e.g. density, hydrophobicity, surface functionalization, particle shape and size, transition temperatures, and mechanical properties) and the ever-increasing levels of pla...
Article
Control over the synthesis of anisotropic nanoparticles is crucial as slight differences in their size, shape, sharpness, or the number of tips in the case of gold nanostars, has an inordinate influence on their properties and functionality for future applications. Herein, we show that the supplier and purity of polyvinylpyrrolidone (PVP) can signi...
Article
The study of plastic particles, particularly those in the micro-, sub-micro-, and nano-size ranges, within food and beverages has gained increasing interest within recent years. However, many analytical techniques have limits of detection which hinder their use for the study of these particles in these sample matrices. In addition, remaining contam...
Article
Full-text available
Small plastic particles such as micro- (<5 mm), sub-micro- (1 µm–100 nm) and nanoplastics (<100 nm) are known to be ubiquitous within our surrounding environment. However, to date relatively few methods exist for the reliable detection of nanoplastic particles in relevant sample matrices such as foods or environmental samples. This lack of relevant...
Article
Nanoparticles (NPs) have attracted considerable attention in various fields, such as cosmetics, the food industry, material design, and nanomedicine. In particular, the fast-moving field of nanomedicine takes advantage of features of NPs for the detection and treatment of different types of cancer, fibrosis, inflammation, arthritis as well as neuro...
Article
Full-text available
Cellular surface recognition and behavior are driven by a host of physical and chemical features which have been exploited to influence particle–cell interactions. Mechanical and topographical cues define the physical milieu which plays an important role in defining a range of cellular activities such as material recognition, adhesion, and migratio...
Article
Full-text available
Upon dissolution of silver nanoparticles, silver ions are released into the environment, which are known to induce adverse effects. However, since dissolution studies are predominantly performed in water and/or at room temperature, the effects of biological media and physiologically relevant temperature on the dissolution rate are not considered. H...
Article
Full-text available
Silica nanoparticles (NPs) are widely used in various industrial and biomedical applications. Little is known about the cellular uptake of co-exposed silica particles, as can be expected in our daily life. In addition, an inflamed microenvironment might affect a NP's uptake and a cell's physiological response. Herein, prestimulated mouse J774A.1 ma...
Article
Full-text available
Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well‐understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease o...
Article
Full-text available
Evaluating nanomaterial uptake and association by cells is relevant for in vitro studies related to safe-by-design approaches, nanomedicine or applications in photothermal therapy. However, standard analytical techniques are time-consuming, involve complex sample preparation or include labelling of the investigated sample system with e.g. fluoresce...
Article
Nanocomposite materials benefit from the diverse physicochemical properties featured by nanoparticles, and the presence of nanoparticle concentration gradients can lend functions to macroscopic materials beyond the realm of classical nanocomposites. It is shown here that linearity and time‐shift invariance obtained via the synergism of two independ...
Cover Page
Full-text available
In article number 1900101, Barbara Rothen‐Rutishauser and co‐workers dope solar fluids used in solar thermal energy with polydopamine/bovine serum albumin nanoparticles. The solar fluids produced are circulated through a 3D printed collector, whereby the addition of these nanoparticles results in an efficiency temperature increase of the solar flui...
Article
Full-text available
The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose-response relationship is still a challenge due to the analytical difficulty of quantifying the dose delivered to cells. An approach to accurately quantify CNT doses for submerged in vitro adherent cell culture systems using UV-VIS-near-infra...
Article
Full-text available
Polydopamine can form black nanoparticles and has recently been gaining attention due to its extraordinary heating properties upon excitation with light. Herein, polydopamine hybrid nanoparticles are synthesized in different sizes and subsequently added to a solar fluid to analyze heating ability. The solar fluids with the differently sized hybrid...
Article
Taylor dispersion is a microfluidic analytical technique with a high dynamic range, and therefore is suited well to measuring the hydrodynamic radius of small molecules, proteins, supramolecular complexes, macromolecules, nanoparticles and their self-assembly. Here we calculate an unaddressed yet fundamental property: the limit of resolution, which...
Article
The efficacy of magnetic hyperthermia treatment depends on the optimal available magnetic nanoparticles (MNPs) that are excited in a given alternating magnetic field and viscosity of the region of interest. In this regard, assessing the relevant relaxation parameters is of upmost importance and could improve the speed of development of efficient ap...
Article
The physicochemical properties of nanoparticles (NPs) strongly rely on their colloidal stability, and any given dispersion can display remarkably different features, depending on whether it contains single particles or clusters. Thus, developing efficient experimental methods that are able to provide accurate and reproducible measures of the NP pro...
Article
The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs was not a mechanism to reduce the particle load. Biopersistent NPs such as silica and gold remained in cells for a prolonged time. The only reduction of the intracellular NP nu...
Article
Taylor dispersion is capable of measuring accurately the hydrodynamic radius over several orders of magnitude. Accordingly, it is now a highly competitive technique dedicated to characterizing small molecules, proteins, macromolecules, nanopar-ticles and their self-assembly. Regardless, an in-depth analysis addressing the precision of the technique...
Poster
The detection and characterisation of nanomaterials (NMs) in cellular environments can be challenging, as NMs are likely to be uptaken and associated - possibly causing NM clustering. Especially in nanomedicine and nanotoxicology determination of NM association rates is an important aspect. Various methods exist, which allow the analysis of NM-cell...
Article
Poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) (F127) hydrogels have been used to deliver nitric oxide (NO) topically in biomedical applications. Here, the effect of F127 microenvironments on the photochemical NO release from S-nitrosoglutathione (GSNO) was investigated in F127 solutions 7.6 wt% 15 wt% and 22.5 wt% at...
Article
Magnetic hyperthermia for cancer treatment has gained significant attention in recent years, due to its biocompatibility of applied nanoparticles and the possibility for spatially localized heating in deep tissues. Clinical treatments use nanoparticle concentrations of 112 mg Fe/mL, while the concentrations experimental studies have addressed are c...
Article
Controlling S-nitrosothiols decomposition, with the consequent release of nitric oxide, is a topic of great research effort. Nitrosomercaptopyridine (SNO+) incorporation into cucurbit[7]uril cavity results in a large increase of its nitrosation equilibrium constant. This effect being a consequence of the stabilization of organic cations by formatio...
Article
Full-text available
The synthesis of anisotropic metallic nanoparticles (NPs) has been a field of intense and challenging research in the past decade. In this communication, we report on the reproducible and highly controllable synthesis of monodisperse branched gold nanoparticles in a droplet-based microfluidics platform. The process has been automated by adapting tw...
Article
The synthesis of anisotropic metallic nanoparticles (NPs) has been a field of intense and challenging research in the past decade. In this communication, we report on the reproducible and highly controllable synthesis of monodisperse branched gold nanoparticles in a droplet-based microfluidics platform. The process has been automated by adapting tw...
Article
S-nitrosothiols (RSNOs) are carriers of nitric oxide (NO) and have important biological activities. We propose here the use of gold nanoparticles (AuNPs) and NO-selective amperometric microsensor for the detection and quantification of S-nitrosoglutathione (GSNO) as a step towards the determination of plasma RSNOs. AuNPs were used to decompose RSNO...
Article
Full-text available
Nitric oxide (NO)-mediated vasodilation plays a key role in gastric mucosal defense, and NO-donor drugs may protect against diseases associated with gastric mucosal blood flow (GMBF) deficiencies. In this study, we used the ex vivo gastric chamber method and Laser Doppler Flowmetry to characterize the effects of luminal aqueous NO-donor drug S-nitr...
Article
The incorporation of nitric oxide (NO) photodonors into nontoxic vehicles may allow for the tunable control of topical NO release to treat pathological conditions associated with the impairment of NO production in the dermal vasculature. Pluronic F127 is a PEO-PPO-PEO triblock copolymer that is used as a nontoxic vehicle that undergoes thermally re...
Article
We report herein the design, preparation and characterisation of a new, water soluble, nanoplatform for the light-triggered release of nitric oxide (NO). This nanoconstruct has been achieved by exploiting a layer-by-layer approach to assembly a polyelectrolyte modified with a NO photochemical precursor, around citrate stabilized gold colloidal temp...
Article
Full-text available
We intent to present an overview of the available catalysts for the carbon–carbon cross-coupling reactions based on supported palladium (Pd) nanoparticles (NPs). We begin this perspective with a brief introduction about the cross-coupling reactions and the mechanistic implications of using Pd NPs as catalyst, i.e. heterogeneous versus homogeneous c...
Article
A major problem associated with nitric oxide (NO) donors is the release of the desired amount of NO at a specific site. A number of platforms have been developed for the regulation of NO dosage. We present the use of citrate stabilized gold nanoparticles as a platform to regulate NO release. Due to the affinity between gold and thiols, the characte...
Article
Full-text available
We report a systematic study of the surface modification of gold and silver nanoparticles with DL-penicillamine (PEN) and N-acetyl-DL-penicillamine (NAP), motivated by the possibility of inducing pH-controlled reversible nanoparticleassembly. The interaction of PEN and NAP with the metal nanoparticle surface was studied by isothermal titration calo...
Article
A hybrid system comprising bimetallic nanoparticles supported on carbon nanotubes (CNTs) was engineered to maximize the surface-enhanced Raman scattering signal from solution by generating a high density of hot spots with reproducible enhancing activity and long-term colloidal and optical stability. CNT@AgAu was employed as a bifunctional material...

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Projects (3)
Project
Dear Colleagues, Nanoparticles show considerable potential for applications in many technological sectors, including pharmaceuticals, medical diagnostics, cosmetics, and food technology. Either with purpose or unintentionally, nanoparticles will eventually come in contact with physiological and biological matrices, such as intra- and extracellular fluid and blood plasma, which themselves are complex colloidal media with a multitude of components that readily interact with nanoparticles. Accordingly, the physicochemical properties of the particles, such as physical stability and chemical composition, may be affected, which impacts functionality and integrity and may result in unintended adverse reactions. Owing to such interactions, the behavior and the characterization of the particle system should not be decoupled from the given physiological/biological matrix. This Special Issue aims to collect studies addressing the characterization of nanoparticles and interactions in such environments. Experimental, theoretical, and computational studies addressing physicochemical factors, such as particle concentration, size distribution, shape, chemical composition, surface chemistry, porosity, particle elasticity, mass density, and surface charge, are most welcome. Dr. Sandor Balog Dr. Patricia Taladriz-Blanco Guest Editors