Patrícia Raquel Moreira

Patrícia Raquel Moreira
Universidade Católica Portuguesa | UCP · Centro de Investigação em Ciência e Tecnologia das Artes (CITAR)

PhD

About

53
Publications
15,712
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340
Citations
Citations since 2017
34 Research Items
184 Citations
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Publications

Publications (53)
Article
Full-text available
Microbial communities colonising outdoor sculptures form intricate and dynamic ecosystems, which can accelerate the deterioration processes of the artworks and pose challenges to their conservation. In this study, the bacterial and fungal communities colonising the surfaces of five contemporary outdoor sculptures were characterised by high-throughp...
Article
Dyed effluents from textile industry are toxic and difficult to treat by conventional methods and biotechnological approaches are generally considered more environmentally friendly. In this work, yeast strains Candida parapsilosis, Yarrowia lipolytica and Candida pseudoglaebosa, isolated from wastewater treatment plants, were tested for their abili...
Article
Flow cytometry was evaluated for the first time for the microbiological characterisation of samples collected from the surfaces of stone and mortar sculptures. The study was carried out on outdoor sculptures located in the district of Porto (Portugal), from which samples were collected using swabs and poly(2-hydroxyethylmethacrylate) (poly(HEMA)) c...
Article
Full-text available
The cleaning or removal of manganese stains on Cultural Heritage has not been much tested or successful so far. The aim of this article was to assess a new green cleaning gel for Mn-rich black-blue stains on different substrates. The black-blue stains were characterized at optical and chemical level through colour-related data, optical microscope,...
Article
ISEP Museum was founded in 1999. It stores the memory of industrial school dated from the second half of XIX century. The education, at that time, was very practical and the methodology was based on models and tools. The collections are formed by 3 main typological groups: objects, prints/drawings and photos, a total of about 10 thousand pieces. Th...
Article
Full-text available
The possibility of contemporary mural paintings to be “tagged” by vandals, with spray and/or markers, represents a serious problem for the conservation of urban art. The present study aims to define the applicability of a protective coating on murals’ surface to preserve them against vandalism. The research has been focused on anti-graffiti product...
Article
Full-text available
The outdoor sculpture of the first Portuguese king, D. Afonso Henriques (c. 1109-1185 AD), placed in Guimarães (North Portugal), is one of the most emblematic national sculptures. Created in 1887 by António Soares dos Reis, it possesses a remarkable symbolic value in the presumed birthplace of the king. In addition to the artistic and heritage impo...
Preprint
The outdoor sculpture of the first Portuguese king, D. Afonso Henriques (~1109 – 1185 AD), placed in Guimarães (North Portugal) is one of the most emblematic national sculptures. Created in 1887 by António Soares dos Reis, it possesses a remarkable symbolic value in the presumed birthplace of the king. In addition to the artistic and heritage impor...
Preprint
Full-text available
The cleaning or removal of manganese stains on Cultural Heritage has not been much tested or successful so far. The aim of this article was to assess a new green cleaning gel for Mn-rich black-blue stains on different substrates. The black-blue stains were characterized at optical and chemical level through RGB data, colorimetry, optical microscope...
Article
Full-text available
This article discloses a rare and outstanding type of Mn-rich black-blue patina found on mural painting and granite ashlars located in the church of Sta. Marinha, north of Portugal, and conjectures the phenomenon associated to the appearance of such patinas in different surface materials. This Mn-patina reported on mural painting and their origin i...
Article
Full-text available
Exposure to an atmosphere rich in volatile pollutants can endanger collections in museums, galleries, libraries or archives. Monitoring volatile pollutants in museum microclimates remains an unusual practice in most institutions. Volatile organic compounds (VOCs) can be released by many sources, including the artefacts’ materials themselves. The pr...
Article
Full-text available
Exposure to an atmosphere rich in volatile pollutants can endanger collections in museums, galleries, libraries or archives. Monitoring volatile pollutants in museum microclimates remains an unusual practice in most institutions. Volatile organic compounds (VOCs) can be released by many sources, including the artefacts’ materials themselves. The pr...
Article
Full-text available
The introduction of new guidelines and regulations on chemical production, use, treatment or disposal demanded the development of greener alternatives to conventional products and methodologies. This review briefly presents some of the most promising innovative systems applied in the cleaning of cultural heritage-lasers, green solvents, microemulsi...
Article
In northern Portugal it was identified that some mural paintings from the 15th and 16th centuries disclose a chromatic alteration in the form of black stains possibly of microbiological origin. In the search for its cause, samples were collected and microorganisms were isolated from selected areas of the pictorial sets of three case studies that di...
Poster
Full-text available
In São Martinho do Peso Church (Bragança), some 16th century mural paintings were discovered behind the altars in the main chapel and in both sides of the nave. The two murals in the nave walls were detached in 2002 and applied onto other supports, hanging nowadays in the lateral walls of the nave. In light of the conservation principles from the 1...
Poster
Full-text available
In order to understand the complex microstructure of the mural paintings multi-layers, a novel method of analysis has been applied from the field of medicine into the cultural heritage. X-ray micro focus computer tomography (micro-CT) is a non-destructive imaging technology. It can scan a sample from any angle, characterising its entire volume and...
Article
Full-text available
This paper aims to highlight the museography of Lina Bo Bardi for exhibiting paintings at the Museum of Art of São Paulo, MASP (Brasil), one of the most relevant collections of European art in a museum in the southern hemisphere. Analysed here is the evolution of glazed paintings, different glasses as the different types of backing board used in t...
Article
The microbiome has become one of the most recognizable research subjects and presences in the headlines of news and scientific articles published in almost every biological science area in the last few years. The steady decline in the price of DNA sequencing has enabled metagenomics, community analysis and genome sequencing to enter routine researc...
Article
Outdoor sculptures are part of the sociocultural identity of cities, but are extremely vulnerable to deterioration due to exposure to harsh environmental conditions and climate change. Furthermore, deterioration by micro-organisms renders urgent the need to design protective coatings. This research proposes an integrated methodology for the develop...
Article
Full-text available
The objective of this paper is the presentation of the research strategies adopted and results of the ongoing BIONANOSCULP research project that is aiming to develop solutions in the area of sustainable nanomaterials, which are non-invasive and high-performance in their preventive conservation approach. An integrated methodology was designed as a h...
Presentation
Full-text available
O problema das alterações cromáticas (manchas negras) nas pinturas murais dos sécs. XV-XVI afectas a igrejas de granito, situadas no Norte de Portugal.
Poster
Full-text available
This research project aims to determine the characteristics of the chromatic alterations present in mural paintings, focusing in defining the responsible chemical compounds that will allow the testing of innovative solutions for their elimination as well as preventive conservation methodologies applied to non-controlled environmental settings
Article
Full-text available
Bacterial strains were isolated from cassava-derived food products and, for the first time, from cassava by-products, with a focus on gari, a flour-like product, and the effluents from the production processes for gari and fufu (a dough also made from cassava flour). A total of 47 strains were isolated, all of which were tested to determine their r...
Poster
Full-text available
The research comes to a focus on three case studies: Santa Eulália Church of the Monastery of Arnoso (Vila Nova de Famalicão); São Tiago of Valadares Church (Baião); and Santa Cristina of Serzedelo Church (Guimarães). The three Romanic churches, made of porphyritic granite (biotitic) with coarse grain, fall within rural environments as perceived by...
Article
Enzymatic discolouration of extracellular pigments produced in liquid media by a selected fungal isolate from Portuguese mural paintings was attempted both in solution and on solid support. A promising versatile peroxidase from Bjerkandera adusta was tested and presented interesting discolouration ability, although smaller than the values obtained...
Poster
Full-text available
The main objectives of this investigation were to contextualize the relic in its material, technical and anthropological significance. The research focused on the skeletal remains and the mounting technique of the entire set (bones and image), as also, on the ground and paint layers from Saint Aurelius face. The historic textiles with their decorat...
Poster
Full-text available
In indoor mural paintings such as in churches, cathedrals and museums, fungi and other microorganisms such as bacteria and microalgae are commonly isolated microorganisms and responsible for the deteriorating effect. However, this study was directed to the microorganisms that were considered to be the primary responsible for the black stains on sto...
Poster
Full-text available
Our study focuses on the chromatic alterations of the wall paintings’ surfaces related to the presence and growth of microorganisms. The removal of the dark coloured stains from wall paintings is extremely difficult. Although treatment with biocides eliminate microorganisms, they are ineffective in cleaning the black pigmentation resulting of their...
Conference Paper
Full-text available
Pieza ejemplar del culto de las reliquias en Portugal en el siglo XVIII, San Aurelio mártir es una de las dos imágenes-relicario pertenecientes a la Catedral de Oporto, Portugal. Dispuesta en el interior de una urna de cristal y madera dorada la imagen, de tamaño natural, está representada como un "mártir-soldado", elegantemente vestido a la romana...
Article
Full-text available
Abstract A number of enzymes have applications in the textile sector, as several natural fibres (e.g. cotton, wool and flax) can be subjected to processing using such natural biocatalysts. In the latest two decades, demand for industrial enzymes has increased considerably; however, the textile industry requires highly stable enzymes, and good perfo...
Conference Paper
Full-text available
A workflow based on the Taverna Workbench® software and tentatively named WPSA was developed to perform a generic protein sequence analysis eliminating the need to cut and paste data throughout web applications. The program performs a homology search, a multiple sequence alignment and a phylogeny analysis using PHYLogeny Interface Package (PHYLIP)....
Article
A versatile peroxidase, purified from a novel strain of Bjerkandera sp. (B33/3), was tested for its reactivity on a lignin fraction obtained from straw pulping. The effects of such processing parameters as reaction time, pH, and lignin:enzyme ratio were evaluated. Gel filtration chromatography was employed to characterise the molecular mass distrib...
Article
From the extracellular fluid of a novel strain of Bjerkandera sp., it was isolated, purified and identified the main enzyme responsible for Remazol Brilliant Blue R dye decolourisation. Such an enzyme is able to oxidise manganese, as well as veratryl alcohol and 2,6-dimethoxyphenol in manganese-independent reactions; hence, it can be included in th...
Article
The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology...
Article
A novel strain of Bjerkandera sp. (B33/3), with particularly high decolourisation activities upon Poly R-478 and Remazol Brilliant Blue R (RBBR) dyes, was isolated. The role of the ligninolytic extracellular enzymes produced by this strain on decolourisation of RBBR was studied in some depth. The basis of decolourisation is an enzyme-mediated proce...
Article
Pitch is a general term for the low molecular weight, oleophilic materials extracted from wood, which cause a number of technical problems during pulp and paper processing and decrease pulp and paper quality. Furthermore, due to its high toxicity they are also responsible for severe environmental problems when present in the mill waste waters. Pine...
Article
Full-text available
Egas Moniz Hospital, Lisbon, Portugal. To evaluate the Ligase Chain Reaction (LCx) Mycobacterium tuberculosis Assay for the direct detection of M. tuberculosis complex in respiratory specimens after smear observation, and its suitability for non-respiratory clinical specimens. Analysis of 156 specimens collected from 123 patients with pulmonary tub...

Questions

Questions (2)
Question
I've been having problems with total DNA extraction from Malassezia sp., other than Malassezia furfur. Any suggestions for methodologies?

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Projects

Projects (9)
Project
The constant exposure of urban outdoor public sculptures to environmental elements places them in a high-risk group within the cultural heritage property of cities, regions and countries. In addition to the climatic elements and pollution, biological agents have an important role in the deterioration of cultural heritage causing aesthetic, bio-geophysical and biogeochemical damage. The microorganisms responsible for the biodegradation of urban sculptures are different according to the materials present, and often mature ecosystems with bacteria, fungi, lichens and micro-algae are found growing on the surface of art works. Since materials may vary from metals like bronze or iron, or stone-based such as granite, marble or limestone and also include materials of human creation as are the ceramics and cement, the variability of the elements involved in biodeterioration is also high. From the point of view of conservation, the work in this type of objects, is either treatments to eradicate responsible agents, or for preventing their growth. We can also include in this risk group, the archaeological materials that also have similar conservation problems to those of outdoor sculptures. It is precisely in preventive conservation, that is located the scope of this research project. The main objective of this project is the development of nanofilms for application as protective layers with antimicrobial effects, possibly also with incorporation of anti-UV effects. Principal Contractor: Catholic University of Portugal (UCP) PI: Patrícia Moreira The innovation level is high but supported by the vast experience of CITAR-UCP and CBQF-UCP workgroup with experience in biodeterioration of cultural objects and preventive conservation. Partner CICECO-UA is a center of excellence for analytics regarding biomaterials. Partner SECYR-UAM will also be involved in final stages of product evaluation. The BIONANOSCULP project is ambitious and will bring important developments in the area of innovative nanomaterials, sustainable, non-invasive and high-performance in the area of conservation and archaeology, very specifically in the area of outdoor sculpture. This project will certainly have a strong impact on conservation, archaeology and society. This research work is supported by national funding from FCT- Fundação para a Ciência e a Tecnologia, I.P. (PTDC/EPH-PAT/6281/2014).
Project
Colaboração no projeto “ BIONANOSCULP: Development of BIONANOmaterials for anti-microbial coating of metal and stone-based outdoor SCULPtures”, aprovado pela Fundação para a Ciência e a Tecnologia (FCT), e financiado por fundos nacionais através da FCT/MCTES (PIDDAC). Este projeto está a decorrer na Universidade Católica Portuguesa, no CBQF – Center for Biotechnology and Fine Chemistry, da Escola Superior de Biotecnologia, no polo da Asprela, no Porto. A atividade de pós-doutoramento compreende a modelação tridimensional de um conjunto de obras de arte urbana, dispersas entre o Porto e Santo Tirso, e que vão ser alvo de ensaios no projeto BIONANOSCULP. O objetivo específico da nossa iniciativa consiste em produzir mapeamentos 3D das obras em apreço, de modo a caracterizar a localização espacial das principais patologias, extensão das colónias de fungos e líquenes e o posicionamento exato dos testes de biocidas. A coordenação dos trabalhos é da Professora Doutora Patrícia Moreira da Costa. As peças são constituídas por vários materiais: material pétreo; ligas metálicas e materiais contemporâneos. O elenco de peças é o seguinte: ● Rosalía de Castro (1951) de Salvador Barata Feyo. Granito Rosa. Praça da Galiza, Massarelos, Porto. ● Afonso de Albuquerque (1930) de Diogo de Macedo. Pedra Ançã. Largo de D. João III, Lordelo do Ouro, Porto. ● Movimento (?) de Jorge Ulisses (prova de agregação). Mármore. Jardim da Faculdade de Belas Artes, UP, Porto. ● Repouso (1953) de Gustavo Bastos. Argamassa de Cimento. Jardim da Faculdade de Belas Artes, UP, Porto. ● O guardador do Sol de José Rodrigues (1953?). Bronze patinado. Jardim da Faculdade de Belas Artes, UP, Porto. ● Eu espero de Fernanda Fragateiro (1999). Aço inox. Museu Internacional de Escultura Contemporânea de Santo Tirso, Santo Tirso. ● Sol, Lua e Vento (1997) de Satoru Sato. Granito Cinza. Museu Internacional de Escultura Contemporânea de Santo Tirso, Santo Tirso.