Patrícia Assunção

Patrícia Assunção
Instituto Tecnológico de Canarias · Biotechnology

PhD Veterinary Medicine

About

56
Publications
33,720
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Introduction
I've a PhD in Veterinary Science. Previously, I worked in the research area of Veterinary Epidemiology and Preventive Medicine (Mycoplasmas) and now I'm working in the Biotechnology Departament of ITC with Microalgae, where I'm responsable for the molecular biology lab.
Additional affiliations
January 2012 - present
January 2004 - December 2008

Publications

Publications (56)
Book
Full-text available
The culture of the dinoflagellates Gambierdiscus and Fukuyoa is one of the most challenging tasks to succeed in when dealing with marine micro-organisms in laboratory. Overall, the species of these two genera are characterised by slow growth (with maximum growth rates below 0,5 divisions per day) and some of them have narrow tolerance ranges of sal...
Book
Full-text available
The culture of the dinoflagellates Gambierdiscus and Fukuyoa is one of the most challenging tasks to succeed in when dealing with marine micro-organisms in laboratory. Overall, the species of these two genera are characterised by slow growth (with maximum growth rates below 0,5 divisions per day) and some of them have narrow tolerance ranges of sal...
Article
Full-text available
Arthrospira (known commercially as Spirulina) is an edible cyanobacterium traditionally used for centuries as human food by various cultures. Its biochemical profile includes many bioactive molecules with enormous potential in human health. The aim of this paper is to systematically review the scientific evidence about the effects of dietary Arthro...
Article
Full-text available
Microalgae constitute an excellent feedstock of valuable compounds which have varied applications in the nutraceutical and pharmaceutical industries. In this study, the isolation of two microalgal strains, DunaDZ2 and DunaDZ3, from Sidi Ameur salt lake was done by streaking water on solid f/2 medium. The strains were subjected to morphological iden...
Article
Full-text available
Algeria has a high algal biodiversity, which is of great interest and should be exploited. Isolation of Dunaliellastrain DunaDz1 from Sidi Ameur Lake in Algeria was carried out in this study. The aim of this research was to identify the isolated strain by morphological and molecular taxonomy using ITS and rbcL genes. The effect of two stress factor...
Article
Full-text available
Microalgal biotechnology is an innovative sector in the field of biotechnology and has evolved exponentially in the last 100 years. With the aim of finding out the current situation of the sector and its development, patents on microalgal biotechnology were surveyed in Espacenet, the European Patent Office database. The objective of this study was...
Article
Full-text available
Flow cytometry (FC) is a straightforward, highly specific and sensitive procedure, which provides objective and quantitative recording of fluorescent signals from individual cells. FC has been used widely in the area of aquaculture research due mainly to the development of monoclonal antibodies (mabs). Phagocyte activity of head–kidney macrophages...
Article
Full-text available
The lipid extractability of 14 microalgae species and strains was assessed using organic solvents (methanol and chloroform). The high variability detected indicated the potential for applying this parameter as an additional criterion for microalgae screening in industrial processes such as biofuel production from microalgae. Species without cell wa...
Book
Full-text available
La finalidad del proyecto BANGEN es promover el desarrollo y uso de las Metodologías de Biología Molecular, basadas en el análisis de ADN, con la finalidad de establecer estrategias de rápida respuesta en la investigación de organismos marinos y gestión de la biodiversidad, así como establecer la red de colaboración y transferencia científico-tecno...
Chapter
Full-text available
El fitoplancton es el conjunto de los organismos acuáticos autótrofos del plancton, que tienen capacidad fotosintética y que viven dispersos en el agua. Son responsables del 95% de la productividad primaria de los océanos, tienen la capacidad de fijar el CO2 atmosférico y producen el 50% del oxígeno necesario para la vida terrestre. Está constituid...
Article
Full-text available
Mycoplasma hyopneumoniae is involved in the porcine enzootic pneumonia and respiratory disease complex; therefore, the search for new treatment options that contribute to the control of this organism is relevant. The minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations of tylvalosin and 19 other antimicrobial agents agai...
Data
Proyecto ALGALIMENTO de explotación comercial de microalgad
Data
Proyecto ALGALIMENTO de explotación comercial de microalgas
Article
Full-text available
Proyecto ALGALIMENTO. Explotación comercial de microalgas
Article
The effects of Crypthecodinium cohnii (Cryp.), Chlorela spp. (Chlo.) and Isochrysis galbana (Iso.) addition to milk replacer on goat kids and lambs growth were evaluated. About 80 Majorera goat kids (males and females) and 80 Canarian sheep lambs were randomly assigned into four different groups (by specie) according to diet. Control groups were fe...
Article
Full-text available
We analyzed the ITS2 primary and secondary structure (including Compensatory Base Changes (CBCs)) of 17 new Dunaliella strains (11 D. viridis, two D. tertiolecta, and four Dunaliella sp.), and compared these with other Dunaliella sequences available from the ITS2 database to circumscribe their taxonomic position. The ITS2 primary and secondary stru...
Book
Full-text available
Developpment potentiel de nouvelles activistas économiques associées à la biotechnologie aux Canarieset su Maroc
Article
Full-text available
We used an ITS2 primary and secondary structure and Compensatory Base Changes (CBCs) analyses on new French and Spanish Dunallela salina strains to investigate their phylogenetic position and taxonomic status within the genus Dunaliella. Our analyses show a great diversity within D. salina (with only some clades not statistically supported) and rev...
Data
Compensatory Base Changes (CBC) analysis. Compensatory Base Changes (CBCs) between different groups and species within the Dunaliella taxon (Excel file).
Article
Full-text available
Dunaliella acidophila is one of the most extreme acidophiles on earth and is able to survive in highly acidic habitats. This characteristic has made this organism the universal model for the study of abiotic stress. Although D. acidophila is currently circumscribed to the subgenus Pascheria within Dunaliella Teodoresco (Chlorophyceae), its taxonomi...
Article
Full-text available
Articulo divulgativo sobre las potencialidades biotecnologías de las microalgas. Se efctua una breve referencia a los diferentes proyectos desarrollados en este campo en el Instituto Tecnológico de Canarias.
Article
Full-text available
To analyze differences in fat and protein content in cheese whey (CW) manufactured in cheese-making factories and farms, goat CW samples were obtained from 60 cheese-making farms and 20 cheese factories. Gross composition of samples was analyzed by using an MIRIS device (MIRIS Inc., Uppsala, Sweden), whey protein composition was subjected to electr...
Article
Given recent evidence of a possible role of camelids in the epidemiology of diseases caused by mycoplasma species in other ruminants, we examined the prevalence of the mycoplasmas most commonly responsible for contagious agalactia (CA) in small ruminants among the dromedaries (Camelus dromedarius) of Lanzarote. On this island, one of the most arid...
Article
Full-text available
Mycoplasma bovis is a serious, worldwide-spread but often overlooked pathogen causing respiratory disease, mastitis, and arthritis in cattle. In this study we characterize the protein and antigenic profiles of M. bovis field strains isolated in Bosnia and Herzegovina by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting,...
Article
Full-text available
This study was designed to assess the possible effects of mycoplasmas on the quality of milk produced by goat herds in a contagious agalactia (CA) endemic area with absence of classical symptoms. Several factors related to milk quality (percentages of fat, total protein, lactose and total solids, standard plate counts (SPC) and presence of Staphylo...
Article
In order to determine how widespread antibiotic resistance has become to standard treatments, the in vitro susceptibilities of 28 Mycoplasma agalactiae Spanish field isolates to 16 antimicrobial agents were determined using a broth microdilution method. The most effective antimicrobials based on minimum inhibitory concentration (MIC)(90) values wer...
Article
Full-text available
The detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpos...
Article
Two vaccines against Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (large colony type) were developed using inactivated strains selected in previous characterization studies. Formaldehyde and phenol were used as the inactivating agents for vaccines A and B, respectively. Aluminium hydroxide plus purified saponin (Quil-A) were added...
Article
Full-text available
MICs were determined for 15 antimicrobial agents against 37 Mycoplasma putrefaciens isolates. The most effective antimicrobial drug classes were the fluoroquinolones, the tetracyclines, the lincosamide lincomycin, and the macrolides. The susceptibility profile of the isolates correlated with the geographic origin. This is the first report of decrea...
Article
Full-text available
Assunção, P., Machado, M.P., Fe, CD., Ramírez, A.S., Rosales, R.S., Antunes, N.T., Poveda, C. and Poveda, J.B. 2007. Prevalence of pathogens in great white pelicans (Pelecanus onocrotalus) from the Western Cape, South Africa. J. Appl. Anim. Res., 32: 29–32. Great White pelicans (Pelecanus onocrotalus) breed in Africa, Europe and Asia from Greece to...
Article
In this study, flow cytometry was evaluated for the determination of the minimal inhibitory concentrations (MICs) of nine antibacterial agents (enrofloxacin, ciprofloxacin, oxytetracycline, chloramphenicol, tylosin, lincomycin, gentamycin, spectinomycin and streptomycin) against M. hyopneumoniae. Flow cytometry was able to detect Mycoplasma hyopneu...
Article
Two vaccines against Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (LC type) were developed using inactivated strains selected in previous characterization studies. The vaccines differed in terms of the adjuvants used: aluminium hydroxide (vaccine A) or aluminium hydroxide plus purified saponin (vaccine B). These vaccines were teste...
Article
During an unusually long period of bad weather, several outbreaks of caprine contagious agalactia (CCA) were reported in a number of flocks on the island of Lanzarote (Canary Islands, Spain). Clinical and subclinical mastitis in lactating goats and some cases of arthritis and pneumonia in kids were observed in the affected flocks. Mycoplasma capric...
Article
Contagious agalactia (CA) is an infectious syndrome caused by several species of mycoplasma and classically characterized by the triad of mammary, joint and eye symptoms, although further symptoms may also appear. CA is the most serious disease of whatever they affect in small dairy ruminants and so there is an urgent need for administrative bodies...
Article
Full-text available
Flow cytometry has become a valuable tool in different fields of microbiology, such as clinical microbiology, aquatic and environmental microbiology, food microbiology, and biotechnology. It combines direct and rapid assays to determine numbers, biochemical and physiological characteristics of individual cells, revealing the heterogeneity present i...
Article
In vitro susceptibilities of 16 Mycoplasma mycoides subsp. mycoides large colony type field isolates to 15 antimicrobial agents were determined using a broth microdilution method. The most effective antimicrobials were fluoroquinolones, tetracyclines and macrolides, with MIC values under 2 microg/ml. Resistance to nalidixic acid, gentamicin, strept...
Article
Full-text available
In this study, flow cytometry was evaluated for the determination of the minimal inhibitory concentrations (MIC) of seven antibacterial agents (enrofloxacin, ciprofloxacin, gentamicin, streptomycin, chloramphenicol, oxytetracycline, and tylosin) on Mycoplasma (M.) agalactiae. Flow cytometry was able to detect M. agalactiae inhibition from 6 h posti...
Article
Full-text available
Flow cytometry together with SYBR green I and propidium iodide was used to study the effects of enrofloxacin, ciprofloxacin, gentamicin, chloramphenicol, oxytetracycline, and tylosin on four mycoplasma species. Inhibition of mycoplasma growth could be detected by as early as 3 h after the start of treatment. The strongest effect was observed with e...
Article
Mycoplasma mycoides subsp. mycoides (LC) (Mmm LC) and Mycoplasma agalactiae are the most important mycoplasma species involved in the contagious agalactia syndrome. A total of 25 field strains from Spain and the two type strains were analysed by SDS-PAGE and immunoblotting. Two polyclonal antisera (PAbs) raised against a pool of strains of each myc...
Article
The potential of using flow cytometry (FC) in combination with a fluorescent dye (SYBR green-I) for rapidly estimating Mycoplasma mycoides subSPS. mycoides large-colony type (MmmLC) in broth culture was examined. The FC analysis was performed by staining the MmmLC cells with a fluorescent dye, SYBR green-I (SYBR), and the results were compared with...
Article
Full-text available
To gain further insight into the pathogenesis of porcine enzootic pneumonia (PEP), cytokine expression in different pulmonary compartments was examined. Mycoplasma hyopneumoniae (Mh) and proinflammatory and immunoregulatory cytokines (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-alpha) were detected by immunohistochemical methods in p...
Article
Full-text available
Mycoplasmas are the smallest and simplest organisms known. They form a large group of bacteria that can infect humans, animals, and plants. Even though several techniques have been proposed to enumerate mycoplasmas in broth medium, the determination of mycoplasma growth still remains a difficult task. The potential of using flow cytometry (FC) for...
Article
Full-text available
Porcine enzootic pneumonia (PEP), with Mycoplasma hyopneumoniae as the primary agent, is a chronic respiratory disease that causes major economic losses to the pig industry worldwide. The aim of this work was to analyse 18 field strains of M. hyopneumoniae isolated in Gran Canaria (Spain) and the reference M. hyopneumoniae strain by SDS-PAGE and im...
Article
In this work, we report a microbiological survey for Mycoplasma spp. undertaken between 2001 and 2002 in 28 goat herds in Gran Canaria, Spain, an area where contagious agalactia is endemic. All herds were randomly selected and represented approximately 15.5% of the total goat population of the island. A variable number of milk, articular and auricu...
Article
Full-text available
The study was conducted to investigate the mycoplasmal flora in the lungs of pigs with enzootic pneumonia at Gran Canaria (Spain). From 54 pneumonic lungs collected at an abattoir, 85 isolates were cultivated. On the basis of cultural and biochemical characteristics, the isolates were preliminarily identified as Mycoplasma species. Using different...
Article
Full-text available
In the present study we evaluated the potential application of the flow cytometry (FC) technique to determine the growth rates of Mycoplasma hyopneumoniae in a broth medium. The FC analysis was performed using the fluorochromes Syto 9, propidium iodide (PI) or a combination of both dyes and results were compared with those obtained by colour-changi...
Article
Full-text available
Assunção, P., Fe, C. de la, Ramírez, A.S., Sarradell, J.E. and Poveda, J.B. 2004. Characterization of a Mycoplasma agalactiae strain, candidate to an attenuated vaccine. J. Appl. Anim. Res., 26: 1–5.The aim of this work was to study the stability of a M. agalactiae attenuated strain after lyophilization and its characterization by sodium dodecyl su...
Article
Full-text available
An indirect ELISA, using local strains of Mycoplasma agalactiae and Mycoplasma mycoides subspecies mycoides large colony (MmmLC), was applied to evaluate the seroprevalence of M agalactiae and MmmLC in flocks of goats on each of the Canary Islands. In total 3890 samples of serum were collected from 204 flocks. The results indicated that the seropre...
Article
Full-text available
The suitability of 5 agents for the inactivation of different field strains of the four mycoplasma species associated with contagious agalactia syndrome in goats, i.e. Mycoplasma agalactiae, Mycoplasma mycoides subsp. mycoides LC, Mycoplasma capricolum subsp. capricolum and Mycoplasma putrefaciens, was investigated. Immunoprophylaxis of this syndro...

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Projects (5)
Project
MIMAR seeks to improve existing knowledge on species with potential impact on the habitats of the NATURA 2000 Network through actions such as: IDENTIFY PROLIFERATION MECHANISMS IDENTIFY INTRODUCTION VECTORS OFFER PREVENTION, CONTROL AND FOLLOW-UP MECHANISMS https://bit.ly/2yRmKur
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