Patrice Marche

Patrice Marche
French Institute of Health and Medical Research | Inserm · Research Center Institute for Advanced Biosciences

PhD
Senior Advisor at the Institute for Advanced Biosciences

About

305
Publications
23,352
Reads
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6,736
Citations
Citations since 2016
61 Research Items
2736 Citations
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20162017201820192020202120220100200300400500600
20162017201820192020202120220100200300400500600
20162017201820192020202120220100200300400500600
Introduction
1978-81: PhD French fellow.1983-85: post-oc Fogherty, NIH-NIAID, Bethesda, MD. 1985-87: Associate Professor, University Paris 71987-91: Investigator, Institut Pasteur Paris since 1991: Directeur de RechercheINSERM 2005-13: Scientific Advisor of the University Hospital Grenoble. 1996 - 2006: head INSERM U548/CEA-Grenoble; 2007-20: head AICD team, IAB, INSERM U1209/CNRS UMR5309/Grenoble-Alpes University. 2016-20, vice-director IAB. Since 2021 Senior Advisor.
Additional affiliations
September 2016 - present
Institut for Advanced Biosciences
Position
  • Managing Director
January 2007 - present
University Joseph Fourier - Grenoble 1
Position
  • Head of Team
September 2006 - present
University of Grenoble-Alpes, France
Position
  • Managing Director
Description
  • see https://iab.univ-grenoble-alpes.fr//
Education
September 1979 - September 1982
September 1978
Institut Pasteur « Fundamental Immunology » diploma
Field of study

Publications

Publications (305)
Article
Polymeric nanoparticles (NPs) are extremely promising for theranostic applications. However, their interest depends largely on their interactions with immune system, including the capacity to activate inflammation after their capture by macrophages. In the present study, we generated monodisperse poly(ethyl methacrylate (PEMA) NPs loaded with hydro...
Article
Full-text available
The pharmaceutical industry has a pervasive need for chiral specific molecules with optimal affinity for their biological targets. However, the mass production of such compounds is currently limited by conventional chemical routes, that are costly and have an environmental impact. Here, we propose an easy access to obtain new tetrahydroquinolines,...
Article
Full-text available
Background and Aims: Inflammatory bowel diseases are highly debilitating conditions that require constant monitoring and life-long medication. Current treatments are focused on systemic administration of immunomodulatory drugs, but they have a broad range of undesirable side�effects. RNA interference is a highly specific endogenous mechanism that...
Article
Full-text available
Immune checkpoint molecules (ICM) are critical in maintaining immunologic homeostasis and participate in preventing or promoting autoimmune disease development. Exploring a large panel of intrahepatic inhibitory and stimulatory ICM is necessary for drawing a general picture of the immune alterations in autoimmune hepatitis (AIH). Here, we performed...
Article
Full-text available
Hepatocellular carcinoma (HCC) is the most common type of liver cancer. The majority of HCC cases are associated with liver fibrosis or cirrhosis developing from chronic liver injuries.The immune system of the liver contributes to the severity of tissue damage, the establishment of fibrosis and the disease’s progression towards HCC. Herein, we prov...
Article
Full-text available
The purpose of this study is to propose and validate a preclinical in vivo magnetic resonance imaging (MRI) tool to monitor neuroinflammation following ischemic stroke, based on injection of a novel multimodal nanoprobe, NanoGd, specifically designed for internalization by phagocytic cells. First, it is verified that NanoGd is efficiently internali...
Article
Full-text available
Nonviral systems, such as lipid nanoparticles, have emerged as reliable methods to enable nucleic acid intracellular delivery. The use of cationic lipids in various formulations of lipid nanoparticles enables the formation of complexes with nucleic acid cargo and facilitates their uptake by target cells. However, due to their small size and highly...
Article
Full-text available
Background and aims: Inflammatory bowel diseases are highly debilitating conditions that require constant monitoring and life-long medication. Current treatments are focused on systemic administration of immunomodulatory drugs, but they have a broad range of undesirable side-effects. The RNA interference is a highly specific endogenous mechanism t...
Preprint
Full-text available
Nonviral systems, such as lipid nanoparticles, have emerged as reliable methods to enable nucleic acid intracellular delivery. The use of cationic lipids in various formulations of lipid nanoparticles enables the formation of complexes with nucleic acid cargo and facilitates their uptake by target cells. However, due to their small size and highly...
Poster
Full-text available
Nanotechnology has the ability to improve gene therapy by delivering therapeutic nucleic acids. Lipid nanoparticles currently in use for several biomedical applications integrate cationic lipids as carriers to form complexes. An increased surface area to volume ratio and charge of nanocarriers facilitate their interaction with target cells, but thi...
Article
Full-text available
Hepatocellular carcinoma (HCC) can have viral or non-viral causes 1–5 . Non-alcoholic steatohepatitis (NASH) is an important driver of HCC. Immunotherapy has been approved for treating HCC, but biomarker-based stratification of patients for optimal response to therapy is an unmet need 6,7 . Here we report the progressive accumulation of exhausted,...
Article
Full-text available
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, characterized by hepatic steatosis without any alcohol abuse. As the prevalence of NAFLD is rapidly increasing worldwide, important research activity is being dedicated to deciphering the underlying molecular mechanisms in order to define new therapeutic targets. To...
Article
The localized functionalization of pores and channels of micrometric and sub‐micrometric sizes is a bottleneck in surface chemistry. A method for the regioselective chemical functionalization of planar pores is presented, that are, restrictions in microfluidic channels, here made of SiO2‐coated silicon. This strategy, based on bipolar electrochemis...
Article
Full-text available
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related deaths worldwide, and its incidence is rising. HCC develops almost exclusively on the background of chronic liver inflammation, which can be caused by chronic alcohol consumption, viral hepatitis, or an unhealthy diet. The key role of chronic inflammation in the proce...
Article
The aim of the study was to identify the most effective serum tumor markers for early diagnosis of hepatocellular carcinoma based on the combination of diagnostic characteristics and correlations. Materials and Methods. There were observed 55 patients with chronic hepatitis C in the stage of liver cirrhosis with a verified diagnosis of hepatocellul...
Article
Full-text available
Neuroinflammation is a process common to several brain pathologies. Despites its medical relevance, it still remains poorly understood; there is therefore a need to develop new in vivo preclinical imaging strategies to monitor inflammatory processes longitudinally. We here present the development of a hybrid imaging nanoprobe named NP3, that was sp...
Article
We evaluated the possibility of using an experimental model of hepatocellular carcinoma to study oncomarkers of primary liver cancer and compared the diagnostic efficacy of alpha-fetoprotein and osteopontin in the experiment and in clinical practice. Experimental studies were performed on a model of hepatocellular carcinoma induced by administratio...
Article
Full-text available
Gold nanoparticles (AuNPs) have demonstrated outstanding performance in many biomedical applications. Their safety is recognised; however, their effects on the immune system remain ill defined. Antigen-presenting cells (APCs) are immune cells specialised in sensing external stimulus and in capturing exogenous materials then delivering signals for t...
Article
Full-text available
Direct-acting antivirals (DAAs) are highly effective in targeting hepatitis C virus (HCV) infections, but the incidence of HCV-related hepatocellular carcinoma (HCC) remains still high. In this study, we investigated a cohort of HCV-infected patients treated with DAAs who were followed up for 4 years after sustained virological response (SVR) achie...
Method
Full-text available
Using siRNAs to genetically manipulate immune cells is important to both basic immunological studies and therapeutic applications. However, siRNA delivery is challenging because primary immune cells are often sensitive to the delivery materials and generate immune responses. We have recently developed an amphiphilic dendrimer that is able to delive...
Article
Full-text available
Hepatocellular carcinoma (HCC) is the most common primary liver malignancy with one of the highest mortality rates among solid cancers. It develops almost exclusively in the background of chronic liver inflammation, which can be caused by viral hepatitis, chronic alcohol consumption or an unhealthy diet. Chronic inflammation deregulates the innate...
Preprint
Full-text available
Background: Gold nanoparticles (AuNPs) have demonstrated outstanding performance in various biomedical applications, but their effects on the immune system remain ill-defined. We studied the impact of AuNPs on antigen-presenting cells (APCs) because of their phagocytic capacity that allows the accumulation of exogenous materials. As models, we used...
Article
Full-text available
In this response, we reply to the concerns raised by Weber et al. on our recently published article (Boudard et al. 2019) that provided evidence that chronic oral exposure to a form of synthetic amorphous silica (SAS) used as the food additive E551 (i.e., precipitated silica as NM-200) results in renal and liver lesions in mice. The letter criticiz...
Article
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Background: MicroRNA plays a large role in regulating the expression of genes involved in carbohydrate, lipid metabolism, and iron metabolism in the body. In experimental studies it was shown that in addition to regulatory functions, miR-122 is involved in the pathogenesis of hepatitis C, providing the life cycle of the virus in the cell. Aims: to...
Article
The possibility of using an experimental model of hepatocellular carcinoma to study tumor markers of primary liver cancer and a comparative analysis of the diagnostic efficacy of alpha-feto protein and osteopontin in experiment and in real clinical practice has been carried out. Experimental studies were done on the model of hepatocellular carcinom...
Article
Full-text available
Liver cirrhosis in the outcome of hepatitis C is the leading cause of hepatocellular carcinoma (HCC) in the world. Early diagnosis and timely treatment of HCC are important for reducing mortality and increasing life expectancy of patients with hepatocellular carcinoma. To assess the risk of HCC, the definition of alpha-fetoprotein (AFP) in the bloo...
Article
Full-text available
This review summarizes recent advances in micro- and nanopore technologies with a focus on the functionalization of pores using a promising method named contactless electro-functionalization (CLEF). CLEF enables the localized grafting of electroactive entities onto the inner wall of a micro- or nano-sized pore in a solid-state silicon/silicon oxide...
Book
This review summarizes recent advances in micro- and nanopore technologies with a focus on thefunctionalization of pores using a promising method named contactless electro-functionalization (CLEF). CLEF enables the localized grafting of electroactive entities onto the inner wall of a microor nano-sized pore in a solid-state silicon/silicon oxide me...
Conference Paper
Background and aim: Liver cancer is now the second most common cause of cancer related death worldwide, with hepatocellular carcinoma (HCC) accounting for the majority of these cases . 90% of HCC are associated with liver fibrosis or cirrhosis developed from chronic liver injuries.Small animal models represent essential tools in cancer research. As...
Article
Full-text available
Introduction: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. Today, a promising treatment strategy is focused on the enhancement of antitumor immune responses by immune checkpoint modification. However, as only 20% of patients with HCC are responders, identification of predictive factors is urgently...
Conference Paper
Background and aim: Liver cancer is now the second most common cause of cancer related death worldwide, with hepatocellular carcinoma (HCC) accounting for the majority of these cases . 90% of HCC are associated with liver fibrosis or cirrhosis developed from chronic liver injuries.Small animal models represent essential tools in cancer research. As...
Article
Full-text available
Abstract Boudard D et al, KIReports 2019 ,doi.org/10.1016/j.ekir.2019.06.007 Introduction Silicon dioxide, produced as synthetic amorphous silica (SAS), is made of nanoparticles (NPs), either present as such or as agglomerates and aggregates, and is widely used in many types of food processes and products as additive. To assess whether repeated, l...
Article
Hepatocellular carcinoma (HCC) is the second leading cause of death in oncological patients. The prognosis of the disease outcome depends directly on its timely detection. Currently, in the majority of countries, the diagnostic algorithm at the preclinical stage of tumor development includes determination of alpha-fetoprotein in combination with in...
Article
Objective the two related TNF members APRIL and BAFF are currently targeted in autoimmune diseases as B‐cell regulators. In multiple sclerosis (MS), combined APRIL/BAFF blockade led to an unexpected exacerbated inflammation in the central nervous system (CNS) of patients. Here, we investigate the role of the APRIL/BAFF axis in the CNS. Methods APR...
Article
Full-text available
Aim. The current study is aimed at determining differences of epidemiological and clinical profiles associated with HCC in patients belonging to ethnic groups of Asians from Mongolia and Caucasians from Asian region of Russia. Materials and methods. The studies were carried out in the cross-border regions of Mongolia and Asian part of Russia (Irkut...
Article
Full-text available
The aberrant expression of human endogenous retrovirus (HERV) elements of the HERV-W family has been associated with different diseases, including multiple sclerosis (MS). In particular, the expression of the envelope protein (Env) from the multiple sclerosis-associated retrovirus (MSRV), a member of HERV-W family and known for its potent proinflam...
Article
Full-text available
Optimizing HIV p24 vaccine responses To date, HIV vaccines have resulted in poor or absent protection. A team led by Fabrice P. Navarro at the CEA LETI use the conserved HIV capsid protein p24 vectorized into cationic nanostructured lipid carriers (NLC-p24) along with NLC-delivered CpG. Owing to their small size, NLCs gain access to lymph nodes and...
Article
Background: Pneumonia is a common disease that is a leading cause of mortality worldwide and frequently requires hospital admission. A large number of infectious agents may be involved and as a result of the potential severity of the infection, a clear and quick diagnostic is mandatory in order to take the appropriate decision for the health of the...
Article
Full-text available
The causes of multiple sclerosis and amyotrophic lateral sclerosis have long remained elusive. A new category of pathogenic components, normally dormant within human genomes, has been identified: human endogenous retroviruses (HERVs). These represent ∼8% of the human genome, and environmental factors have reproducibly been shown to trigger their ex...
Article
Full-text available
The prognosis of patients with advanced hepatocellular carcinoma (HCC) is very poor. The AKT pathway is activated in almost half of HCC cases and in addition, long term exposure to conventional drug treatment of HCC, sorafenib, often results in over-activation of AKT, leading to HCC resistance. Therefore, it is important to assess the safety and th...
Article
Full-text available
Hepatocellular carcinoma (HCC) is the second most common cause of cancer-related mortality worldwide. AKT pathway has been found activated in 50% of HCC cases, making it promising target. Therefore we assess efficacy of the allosteric AKT inhibitor ARQ 092 compared to untreated control and to standard treatment, Sorafenib, in vitro and in vivo ARQ...
Article
New vaccine formulations are still highly anticipated in the near-future to face incoming health challenges, such as emergence or reemergence of severe infectious diseases, immunosenescence associated with elderly or the spread of pathogens resistant to antibiotics. In particular, new nanoparticle-based adjuvants are promising for sub-unit vaccines...
Article
Full-text available
Major sex differences are observed in the prevalence, intensity, and severity of hepatitis B virus (HBV) infection. Here, we investigated degranulation activity of circulating and intrahepatic natural killer (NK) cells from HBV and HCV chronically infected patients before any treatment (n = 125). The frequency of CD107 + NK cells in the female live...
Article
Full-text available
Human endogenous retroviruses (HERV) represent a large part of our genome and the few elements that have retained a potential of expression still remain "dormant" in physiological conditions. In some instances, they can be awakened by environmental factors activating their expression. The best studied conditions of HERV activation are infections ca...
Article
Aim: We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared...
Article
Full-text available
Nanoparticles (NP) physico-chemical features greatly influence NP/cell interactions. NP surface functionalization is often used to improve NP biocompatibility or to enhance cellular uptake. But in biological media, the formation of a protein corona adds a level of complexity. The aim of this study was to investigate in vitro the influence of NP sur...
Data
Supplementary Information: Supplemetary Figure 1. Gating strategy and degranulation of NK cells. A) Gating strategy to investigate degranulation activity of NK cells. Dead cells were excluded and immune cells were identified according their FSC and SSC parameters (left panel). Cells were further gated based on their CD45high+ expression and NK cell...
Article
Full-text available
Peripheral T cell lymphomas (PTCLs) are a heterogeneous entity of neoplasms with poor prognosis, lack of effective therapies, and a largely unknown pathophysiology. Identifying the mechanism of lymphomagenesis and cell-of-origin from which PTCLs arise is crucial for the development of efficient treatment strategies. In addition to the well-describe...