Patompong Saengwilai

Mahidol University | MU ·  Department of Biology

Similar to any microscopic appendages, such as cilia or antennae, phenotyping of root hairs has been a challenge due to their complex intersecting arrangements in two-dimensional (2D) images and the technical limitations of automated measurements. Digital Imaging of Root Traits at Microscale (DIRT/μ) addresses this issue by computationally resolvin...

Similar to any microscopic appendages, such as cilia or antennae, phenotyping of root hairs has been a challenge due to their complex intersecting arrangements in two-dimensional (2D) images and the technical limitations of automated measurements. Digital Imaging of Root Traits at Microscale (DIRT/μ) addresses this issue by computationally resolvin...

Root anatomical phenotypes present a promising yet underexploited avenue to deliver major improvements in yield and climate resilience of crops by improving water and nutrient uptake. For instance, the formation of root cortical aerenchyma (RCA) significantly increases soil exploration and resource capture by reducing the metabolic costs of root ti...

While the adaptation and responses of model plants to phosphorus (P) deficiency are well documented, those of the rubber tree remain unclear. Here, we investigated above‐ and below‐ground responses of rubber tree seedlings over the development of the first to third extension unit in a mesocosm system. Rubber tree seedlings were grown in high and lo...

While ecological roles of rhizosphere arthropods are well documented, little is known about the relationship between the development of plant roots and soil arthropod communities in agroecosystems. In this study, we investigated the effects of maize varieties and their root traits on the diversity and community composition of soil arthropods over t...

The contamination of paddy fields and rice grains by cadmium (Cd) adversely affects human health. Thus, many approaches have been proposed to reduce the accumulation of Cd in rice. Here, we investigate the potential of aqueous Moringa oleifera leaf extract (AMOLE) in decreasing uptake and toxicity of Cd in a popular Thai jasmine rice variety, Khao...

During rhizoremediation process, plant roots secrete the specific exudates which enhance or stimulate growth and activity of microbial community in the rhizosphere resulting in effective degradation of pollutants. The present study characterized cowpea (CP) and mung bean (MB) root exudates and examined their influences on the degradation of total p...

Zinc mining and smelting activities result in cadmium (Cd) and zinc (Zn) contamination in rice grains, causing deleterious impacts on human health and local economies. Here, we investigated the effects of soil amendments, including mixtures of dicalcium phosphate with cattle manure (T1) and leonardite (T2), on soil physicochemical properties as wel...

Background and Aims The utility of root hairs for nitrogen (N) acquisition is poorly understood. Methods We explored the utility of root hairs for N acquisition in the functional-structural model SimRoot and with maize genotypes with variable root hair length (RHL) in greenhouse and field environments. Key Results Simulation results indicate that...

The utility of root hairs for nitrogen (N) acquisition is poorly understood. We explored the utility of root hairs for N acquisition in the functional-structural model SimRoot and with maize genotypes with variable root hair length (RHL) in greenhouse and field environments. Simulation results indicate that long, dense root hairs can improve N acqu...

The contamination of paddy fields and rice grains by cadmium (Cd) adversely affects human health. Thus, many approaches have been proposed to reduce the accumulation of Cd in rice. Here, we investigate the potential of aqueous Moringa oleifera leaf extract (AMOLE) in decreasing uptake and toxicity of Cd in a popular Thai jasmine rice variety, Khao...

Drought is a major constraint in rainfed rice production and root architectural traits are important breeding targets for improving productivity under drought stress. A set of chromosome segment substitution lines (KDML105-CSSLs) and KDML105 were grown in the wet season at two sites (Rice Gene Discovery (RGD) and Ubon Ratchatani Rice Research Cente...

Societal Impact Statement: Micronutrient deficiency or “hidden hunger” is estimated to affect two billion people worldwide and increasing the micronutrient concentration of food could play an important role in tackling this global challenge. Using a combination of imaging techniques and atomic absorption spectroscopy, we describe a link between roo...

Cadmium (Cd) contamination of rice is a serious public health concern in certain parts of the world. Amendment application based on local organic materials (e.g., manures, compost) is considered effective for reducing plant-available Cd in soil. In this study, two Thai rice (Oryza sativa L) varieties, Chorati and Mali Daeng, were grown in Cd-contam...

This study was aimed at isolating indigenous soil bacteria exhibiting cadmium (Cd)-resistance, and characterizing their ability to improve growth and reduce Cd bioaccumulation of Thai rice (Oryza sativa L.) PSL2 seedlings. Repeated enrichment, microorganisms were selectively propagated from agricultural soils receiving dredged sediments that contai...

Drought is a major yield-limiting factor for rice production. In drying soils, plants experience water scarcity and soil compaction. Penetration of deep roots in hard soil can improve crop productivity under drought. It has been shown that stele is an important anatomical predictor for root penetrability. Here, we studied genetic controls of root s...

Premise of the Study The key to increased cassava production is balancing the trade‐off between marketable roots and traits that drive nutrient and water uptake. However, only a small number of protocols have been developed for cassava roots. Here, we introduce a set of new variables and methods to phenotype cassava roots and enhance breeding pipel...

Cadmium (Cd) contamination in paddy fields affects human health because of the consumption of Cd-contaminated rice. In the current study, we demonstrated that variation in root system architecture (RSA) influenced Cd uptake by rice. Rice cultivars consisting of KDML105, Mali Dang, Pitsanulok2 and Niaw San-pah-tawng were assessed in hydroponics and...

- Improving nutrient and water uptake in resource limited soils is a major challenge for agricultural research. - Root hairs are specialized epidermal cells that are important for nutrient uptake from the soil by increasing the root-surface area. - Digital microscopy can record root hairs as images, but has never been used for automated analysis....

Cadmium (Cd) may be toxic to aquatic plants even at modest concentrations, and excessive quantities of zinc (Zn) decrease plant performance. The Cd and Zn phytoremediation potential of several aquatic plant species (Thalia geniculate, Cyperus alternifolius, Canna indica, Eichhornia crassipes, Pistia stratiotes) and one grass species (Vetiveria ziza...

The ability of a mixture of Typha angustifolia and Eichhornia crassipes to remove organics, nutrients, and heavy metals from wastewater from a Thailand fresh market was studied. Changes in physicochemical properties of the wastewater including pH, temperature, chemical oxygen demand, dissolved oxygen, biochemical oxygen demand (BOD), total P, TOC,...

Root architectural traits refer to a spatial configuration of the root system providing a critical role in soil resource acquisition. In past decades, several lines of evidence have shown that maize possesses large phenotypic variations, including root architecture closely relating to physiological utility. Thailand has developed a unique maize ger...

Arabidopsis has been reported to respond to phosphate (Pi) stress by arresting primary root growth and increasing lateral root branching. We developed a system to buffer Pi availability to Arabidopsis in gel media systems by charging activated aluminum oxide particles with low and sufficient concentrations of Pi, based on previous work in horticult...

Root systems have long been neglected in plant breeding due to their inaccessibility and the laborious nature of root studies. Over the past years, root scientists developed the classification system, methodologies and growing systems for high throughput measurements of root traits. We compared root traits obtained with three growing systems for el...

Potassium (K) deficiency limits rice production worldwide. It has been shown that variation in root traits, such as root hair length, influences ion uptake in many plant species. In this study, we explored natural variation of root hair length of twelve rice varieties in a roll-up system. We found a large phenotypic variation for root hair traits r...

Improving nutrient and water uptake in crops is one of the major challenges to sustain a fast-growing population that faces increasingly nutrient limited soils. Root hairs, which are specialized epidermal cells, compromise up to 70% of the total root surface area. Therefore, it is likely that root hairs are important for nutrient uptake from the so...

Cadmium is a toxic metallic element that poses serious human health risks via consumption of contaminated agricultural products. The effect of mixtures of dicalcium phosphate and organic amendments, namely cow manure (MD) and leonardite (LD), on Cd and Zn uptake of three rice cultivars (KDML105, KD53, and PSL2) was examined in mesocosm experiments....

Soil contamination by cadmium (Cd) poses a serious environmental and public health concern. Phytoremediation, i.e., the use of plants to remove contaminants from soil, has been proposed for treatment of Cd-contaminated ecosystems. In this study, we demonstrated the potential of Vetiveria zizanioides, commonly known as vetiver, to serve as an effect...

Greenhouse and field experiments were conducted to evaluate the influence of three different organic amendments (cow manure, pig manure, and organic fertilizer) on the Cd phytostabilization potential of Eucalyptus camaldulensis grown on Cd-contaminated soil. The application of all amendments, particularly organic fertilizer, improved plant growth (...

Drought and suboptimal nutrient availability significantly reduce crop production worldwide. A number of experiments have demonstrated that the phenotypes, measured as architectural, morphological, and anatomical crop root traits, influence the performance of water and nutrient uptake.

Suboptimal nitrogen availability is a primary constraint for crop production in developing nations, while in rich nations intensive nitrogen fertilization carries substantial environmental and economic costs. Understanding root phenes that enhance nitrogen acquisition is therefore of considerable importance. Structural-functional modeling predicts...

In developing nations, low soil nitrogen (N) availability is a primary limitation to crop production and food security, while in rich nations, intensive N fertilization is a primary economic, energy, and environmental cost to crop production. It has been proposed that genetic variation for root architectural and anatomical traits enhancing exploita...