Pascal Jaouen

Pascal Jaouen
University of Nantes | UNIV Nantes · Laboratoire de génie des procédés, environnement, agroalimentaire (GEPEA)

38.09
 · 
Professor (Full)

About

198
Publications
32,549
Reads
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3,411
Citations
Introduction
Pascal JAOUEN, Honorary Director of GEPEA, UMR-CNRS-6144 - Board of directors, Univ. Nantes - VP "Mer-Bretagne-Atlantique Economic Cluster", PMBA, Brest, France - Board of directors SFGP, Paris - Chair, Congress #ISAP2017Nantes & #SFGP2019 Nantes - former director of IUML FR-CNRS-3473 (Institut Universitaire Mer Littoral), 19 labs from Univ. Nantes, CNRS, IFREMER & Centrale Nantes - former director Department "Process & Bioprocess Eng." (Graduate School of Eng. Polytech' Nantes)
Research Experience
January 2012 - present
French National Centre for Scientific Research
Position
  • Director of IUML (FR CNRS 3473) and Deputy-Director of GEPEA (UMR CNRS 6144)
January 1986 - present
University of Nantes
Position
  • GEPEA UMR CNRS 6144
Education
January 1986 - February 1989
Université de Nantes / Ecole Centrale de Nantes
Field of study
  • Bioprocess Engineering (Microalgae harvesting, Protein separation)

Publications

Publications (198)
Article
Full-text available
The microalga Porphyridium cruentum is known to produce many components of interest. One of them is B-Phycoerythrin (B-PE), a water-soluble intracellular pigment used as an immunofluorescent probe. Current methods to extract this molecule involve total cell disruption and lead to a mix of all the water-soluble components. Subsequently, the pigment...
Article
Cross-flow microfiltration and ultrafiltration techniques have become a suitable process for the separation of micro-organisms in a variety of biotechnical applications. In this paper, eight commercial membranes (IRIS, Orelis, Miribel, France) were evaluated for the harvest-ing of two marine microalgae: Haslea ostrearia and Skeletonema costatum, bo...
Article
A widely used technique for cell harvesting is crossflow microfiltration. The relative fragility of microalgae requires choosing a suitable pumping system. Tetraselmis suecica, which is a very mobile alga, is an interesting model for this study. The extent of damage observed in various pumps was measured as a function of the number of passes of the...
Article
Full-text available
The red microalga Porphyridium cruentum is exploited industrially for its exopolysaccharides (EPS) and pigments production. EPS produced by P. cruentum are partially released and dissolved into the surrounding environment, they can be recovered from the culture medium after removing the cells. This paper presents a parametric study of the ultrafilt...
Patent
Procédé de préparation d'un extrait liquide riche en phycocyanine à partir de cyanobactéries ou de micro-algues renfermant de la phycocyanine, comprenant une étape de lyse cellulaire d'une suspension aqueuse des dites cyanobactéries ou micro-algues fraîches, une étape de macération du lysat obtenu, pendant plusieurs heures dans une solution de cati...
Article
For biodiesel applications, microalgae and especially Nannochloropsis sp. are considered as a promising feedstock for lipid production. Main issues are high lipid productivity, but also robust and energy efficient downstream processes. In the biorefinery process, cellular mechanical pretreatment and solvent extraction are investigated nowadays to a...
Poster
use or membrane process for isolating biofunctional exopolysaccharides (EPS) produced from microalgae and cyanobacteria
Article
Recognition of limited resources and increasing environmental pollution has emphasized the need for better utilization of fisheries by-products (e.g., heads, frames, and viscera). The present investigation explores the technical feasibility of “clean technologies”, that is, enzymatic hydrolysis and ultrafiltration to fractionate and concentrate Sep...
Article
This study tested the efficiency of granular filtration using a bilayer sand filter for microalgae removal from culture dilutions ranging from 10,000 to 17,000 cells/mL. The objective is to evaluate the removal capacity of the filter without chemical coagulation. Two filter media, sand and anthracite, with mean grain sizes of 0.395 mm and 1.2 mm re...
Patent
The invention relates to a method for producing membrane polysaccharides from an organism selected from a micro-organisms, unicellular organims and filamentous fungi, said method comprising at least one step of extracting said membrane polysacchraides as well as a smaller-scale extraction of soluble proteins, by mechanical of said organism in a bal...
Technical Report
Full-text available
Marine (= blue) biotechnology, i.e. the utilisation of marine bioresources as targets or sources of biotechnology applications, is a field with massive potential for innovation and economic growth. In the context of rapid climate change and increasing pressure on natural resources, recent advances in methodology and technology, particularly in biop...
Article
The ballast waters from ships pose a major threat to oceans, notably because of the spread of microorganisms. The present study evaluates the techno-economic feasibility of implementing the membrane process to remove microalgae from seawater to be ballasted in a single step during planktonic bloom. The optimal conditions for the microfiltration of...
Article
The present work investigates the fouling mechanisms of PVDF hollow fiber membrane (0.03 μm) during the dead end ultrafiltration at a fixed permeate flux (outside to inside configuration) of complex synthetic seawater composed by humic acids, alginic acids, inorganic particles and numerous salts at high concentrations. Short term ultrafiltration ex...
Conference Paper
Full-text available
Because photosynthetic growth implies an inorganic carbon uptake, photosynthetic microorganisms like microalgae or cyanobacteria can be used to fix CO2 from industrial effluents in a sustainable way. In our work, we investigated the specific case of CO2 issued from a cement plant located in France (Ciment Calcia) for the production of microalgae in...
Article
A study of cell disruption by bead milling for two microalgae, Nannochloropsis oculata and Porphyridium cruentum, was performed. Strains robustness was quantified by high-pressure disruption assays. The hydrodynamics in the bead mill grinding chamber was studied by Residence Time Distribution modeling. Operating parameters effects were analyzed and...
Article
Full-text available
Travaux d'un groupe de réflexion au sein de l'Europole Mer sur le thème des biotechnologies marines.
Article
A one-step chromatographic method for the purification of R-phycoerythrin (R-PE) of Grateloupia turuturu Yamada is described. Native R-PE was obtained with a purity index of 2.89 and a recovery yield of 27% using DEAE-Sepharose Fast Flow chromatography with a three-step increase in ionic strength. The analysis by SDS electrophoresis showed a broad...
Article
Full-text available
Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient close to 0.999, observed during both experimental and validation runs, indicating the validity o...
Article
Full-text available
Dossier: Biotech Marine, une vague bleue d'innovations.Des applications à visée médicale à l’agroalimentaire en passant par la cosmétique, les biotechnologies bleues sont aujourd’hui une importante source de biomolécules actives innovantes, grâce à l’incroyable diversité des macro- et des microalgues mais aussi des champignons marins. Et cela ne fa...
Chapter
Full-text available
Abstract (chapter 3): This study fostered partnership between scientists, shellfish growers, water treatment engineers, economists and regulators. The objective was to optimise land-based post-harvest treatments in such a way that oyster and mussel marketing could continue during toxic blooms of Dinophysis acuminata (DSP) and Alexandrium minutum (P...
Article
Full-text available
The freshwater microalga Chlorella vulgaris was harvested by autoflocculation resulting from the precipitation of magnesium or calcium compounds induced by a slow increase in pH in the absence of CO2 input. Autoflocculation was tested in two culture media with, respectively, ammonium (NH4+) and nitrate (NO3-) ions as nitrogen source. The culture pH...
Article
Full-text available
In diatoms, the main photosynthetic pigments are chlorophylls a and c, fucoxanthin, diadinoxanthin and diatoxanthin. The marine pennate diatom Haslea ostrearia has long been known for producing, in addition to these generic pigments, a water-soluble blue pigment, marennine. This pigment, responsible for the greening of oysters in western France, pr...
Article
Full-text available
In diatoms, the main photosynthetic pigments are chlorophylls a and c, fucoxanthin, diadinoxanthin and diatoxanthin. The marine pennate diatom Haslea ostrearia has long been known for producing, in addition to these generic pigments, a water-soluble blue pigment, marennine. This pigment, responsible for the greening of oysters in western France, pr...
Article
Abstract: Cooking juices are fish industry effluents with high organic and salt contents, typically 10-20 g COD.L-1 and 20-30 g salt.L-1. They contain valuable substances (mainly aromas and proteins) that can be recovered, thereby significantly lowering treatment cost and offering a commercial gain for the food industry. The use of membrane process...
Conference Paper
Full-text available
The preindustrial R&D facility AlgoSolis will be devoted to investigations in representative conditions of industrial applications of microalgae (outdoor production, large-scale) and to the integration of technological bricks defining an industrial exploitation of microalgae [cultivation systems (25 lines), harvesting and downstream processing of b...
Data
Full-text available
Marine ressources contain various compounds such as proteins and pigments, lipids or carbohydrates that are highly valuable in food and feed, cosmetic or nutraceutical sectors ... As reliable and environmental technologies, membranes processes (ultra-,nanofiltration...) have found many uses to concentrate, desalt or pre-purify these compounds . Sev...
Article
Phycoerythrin is a major light-harvesting pigment of red algae, which could be used as a natural dye in foods. The stability of R-phycoerythrin of Grateloupia turuturu and B-phycoerythrin of Porphyridium cruentum in relation to different light exposure times, pHs, and temperatures was studied. Regarding the light exposure time, after 48 h, the redu...
Article
Full-text available
The role of natural compounds of seawater and added particles on mechanisms of membrane fouling and organic matter rejection has been investigated. Ultrafiltration (100 kDa) has been conducted in both dead-end (out/in) and tangential (in/out) modes on polysulfone hollow fibre membranes. The permeate fluxes are approximately three times higher for t...
Article
Reusing supernatant of microalgae culture medium can have inhibitory or toxic effects on the biomass production because of the release of organic metabolites by cells in the culture medium during their growth. This work investigated the impact of Chlorella vulgaris medium recycling on culture productivity, cells quality and accumulation of excreted...
Article
Full-text available
The present study has two main purposes: to investigate the performance of a first-stage sand media filter in a case of several reconstituted algal blooms and to better understand fouling mechanism of sand filter due to micro-algae. The retention efficiency of the filter obtained for 30,000, 50,000 and 145,000 cells/ml alga suspensions of Chlorella...
Article
Full-text available
Numerous studies have demonstrated that the biochemical content of seaweeds varies according to seasonality in a restricted area. In this study, the influence of sampling site on the biochemical composition of the edible red seaweed Grateloupia turuturu Yamada was investigated but not its variation over time. Some differences in water-soluble prote...
Article
Low pressure microfiltration (MF) and ultrafiltration (UF) is used as seawater pretreatment before reverse osmosis membranes for capacity that ranged from 6,700 to 140,000 m per day at Colakoglu Steel Mill (Turkey), Yu-Han (China), Kindasa (Saudi Arabia), Fukuoka (Japan), and Ad Dur (Bahrain). Among all modes of porous membrane filtration, pressure...
Article
Full-text available
When microalgae culture medium is recycled, ions (e.g. Na(+), K(+), Ca(2+)) that were not assimilated by the microalgae accumulate in the medium. Therefore, a growth medium (HAMGM) was developed that included ions that were more easily assimilated by Chlorella vulgaris, such as ammonium one (NH(4)(+)). Recycling performance was studied by carrying...
Article
Full-text available
In this study, a sand filter was used to remove micro-algae from seawater feeding aquaculture ponds. A lab-scale sand filter was used to filter 30,000 cells/mL of Heterocapsa triquetra suspension, a non-toxic micro-alga that has morphological and dimensional (15-20 microm) similarities with Alexandrium sp., one of the smallest toxic micro-algae in...
Article
The present study completes a previous work dedicated to the feasibility to implement, on-board ship, a direct nanofiltration process in order to treat laundry greywaters and recycle 80% to the inlets of the washing machines (Guilbaud et al., 2010). At present, the study investigates the influence of nanofiltration operating conditions on Chemical...
Article
Full-text available
Enzymatic hydrolysis of cuttlefish viscera by Protamex, Pepsin, Delvolase and Multifect Neutral was investigated in a batch reactor during 6 hours with an hourly sampling. The influence of the enzyme used and the hydrolysis conditions (operating pH, temperature) was studied with regards to the extent of proteolytic degradation and to molecular weig...
Article
The present study deals with the specific energetic consumption evaluation of dead-end and tangential ultrafiltration (UF) (100kDa) of salted suspensions on polysulfone membrane in regards of backwash and aeration conditions. For TMP ranging from 0.2 to 0.8bar, it was found that specific energy consumption during tangential UF was always higher tha...
Article
Full-text available
In this study, an original use of immersed microfiltration membranes (mean pore size equal to 0.2μm) is investigated for the total removal of toxic dinoflagellates from seawater. Using a membrane autopsy and fouling model approach and the use of fouling indexes (called Pore Blocking Index, Pore Constriction Index and Cake Filtration Index), three d...
Article
Tuna cooking juices contain high organic load preventing the rejection in the environment without treatment. But the effluents present an interesting fishy odour and it is worth recovering aroma compounds. In this work, two industrial tuna cooking juices were concentrated by nanofiltration. Nanofiltration performance was discussed in terms of perme...
Article
Full-text available
Greywater reuse inside buildings is a possible way to preserve water resources and face up to water scarcity. This study is focused on a technical-economic analysis of greywater treatment by a direct nanofiltration (NF) process or by a submerged membrane bioreactor (SMBR) for on-site recycling. The aim of this paper is to analyse the cost of recycl...
Article
Full-text available
The present work deals with the evaluation of hydrodynamic effects on the ultrafiltration of microalgae suspensions for harvesting or metabolite production by coupling photobioreactors with membrane separation processes. Two ultrafiltration systems were compared: the first was a cross flow ultrafiltration unit equipped with a flat sheet membrane an...
Article
Full-text available
Introduction et objectifs du projet La contamination des denrées d’origine marine par des toxines algales (phycotoxines) a pris depuis quelques années une importance considérable. Ce phénomène est largement lié à l’augmentation du nombre de zones géographiques touchées ainsi qu’à la diversification des espèces toxinogènes et à l’identification de...
Conference Paper
Greywater reuse inside buildings is a possible way to preserve water resource and face up to water scarcity. This study is focused on greywater treatment by a submerged membrane bioreactor (SMBR) for on-site recycling. The aim is to find satisfying operating conditions in order to achieve the best compromise between permeation fluxes and retent...
Article
Full-text available
On-site greywater recycling and reuse is one of the main ways to reduce potable water requirement in urban areas. Direct membrane filtration is a promising technology to recycle greywater on-site. This study aimed at selecting a tubular nanofiltration (NF) membrane and its operating conditions in order to treat and reuse greywater in buildings. To...
Article
Full-text available
BACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was de...
Article
In this study, an original use of low-energy-consuming submerged MF/UF membranes was investigated for the total removal of toxic micro-algae (Alexandrium sp.) from seawater. Experimentally, a non-toxic micro-alga (Heterocapsa triquetra) which has morphological and dimensional (15–20 µm) similarities with Alexandrium sp., one of the smallest toxic m...
Article
Full-text available
The present study deals with the feasibility to implement, on board ship, a direct nanofiltration process in order to treat laundry grey waters and recycle 80% to the inlets of the washing machines. At first, a specific methodology for real laundry grey water production was set up at the laboratory for the purpose. Characteristics of the lab-produc...
Article
The chemical composition and its seasonal variation of the red seaweed Grateloupia turuturu, an invasive macroalgae from Brittany, France, were investigated. Size, ash, protein, lipid, dietary fibre (soluble, insoluble and total), protein pigment (R-phycoerythrin, R-phycocyanin), and fatty acid content were measured in G. turuturu samples collected...
Article
The overall objective of the present work was to estimate by membrane autopsy the level of performances degradation of old reverse osmosis (RO) membranes/modules in order to envisage their reuse. A mechanistic approach using the Spiegler–Kedem–Katchalsky model helps us to observe that the old RO membrane acquired a convective mass transfer. We defi...
Article
On-site greywater recycling is one of the main ways of preserving water resources in urban or arid areas. This study aims to formulate model synthetic greywater (SGW) in order to evaluate and compare the performances of several recycling processes on a reproducible effluent. The formulated SGW is composed of septic effluent to provide indicators of...
Article
Full-text available
In a global context of marine biological resource overexploitation, a better upgrading of fish and shellfish biomass is a challenge for the 21 st century. One of the main and promising issues will be the production of marine bio-ingredients using enzymatic hydrolysis. This paper presents the key steps in the production of enzymatic hydrolysates, su...