Parthasarathi Bhattacharya

Okra leaf curl and yellow vein mosaic disease caused by begomovirus and satellite DNA complexes is a major constraint of okra (Abelmoschus esculentus L.) production in India. We performed recombination analysis, in silico structural characterization of βC1 protein gene from okra leaf curl betasatellites isolated from Hyderabad and discussed the rec...

Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv. Coker 310) plants were developed by using βC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminat...

Tospovirus has emerged as a serious viral pathogen for several crops including tomato. The tomato production is being severely affected worldwide by tospovirus. Some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. Resistance to this virus has been identified...

Cotton is an important crop and its production is affected by various disease pathogens. Monopartite begomovirus associated betasatellites cause Cotton leaf curl disease (CLCuD) in Northern India. In order to access the occurrence and genetic variability of Cotton leaf curl betasatellites, an extensive field survey was conducted in states of Rajast...

A B S T R A C T A severe leaf curl disease of Okra with high disease incidence and loss of production was observed during field survey of various locations of Southern India, and causal organism was identified as Begomovirus and its association was confirmed by polymerase chain reaction (PCR) but also by cloning and sequencing of amplified PCR prod...

A B S T R A C T A severe leaf curl disease of Okra with high disease incidence and loss of production was observed during field survey of various locations of Southern India, and causal organism was identified as Begomovirus and its association was confirmed by polymerase chain reaction (PCR) but also by cloning and sequencing of amplified PCR prod...

Leaf curl and yellow vein mosaic viral disease is the major constraint on okra (Abelmoschus esculentus L.) production in India. Amplified fragment sequence of DNA-β showed highest similarity of 91.7% with Bhendi yellow vein mosaic virus-Tamil Nadu (AJ308425, NC_003405) and lowest similarity of 48.5% with OKLCV (NC_004093), whereas coat protein spec...

The PTM3 (MADS-box) gene characterized from flower of Aspen was ectopically expressed in cotton and was found to result in desirable agronomic traits. Among several transgenic lines, the PTM3 cotton event-10 was found to flower significantly earlier than the control and yield better [Ramachandran et al. (2011)]. We report our findings on performanc...

In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L(-1) 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observ...

Agrobacterium-mediated genetic transformation was performed using embryonic axes explants of pigeon pea. Both legume pod borer resistant gene (cry1Ac) and plant selectable marker neomycine phosphor transferase (nptII) genes under the constitutive expression of the cauliflower mosaic virus 35S promoter (CaMV35S) assembled in pPZP211 binary vector we...

The PTM3 gene of Aspen was ectopically expressed in cotton to explore the opportunity to introduce desirable agronomic traits with the potential to improve yield and modify the duration of the parent cotton variety. Sixty-seven transgenic cotton lines expressing Aspen PTM3 (MADS box) gene were developed. The transgenic cotton lines expressing PTM3...

Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in many developing countries. In Ind...

Coleus forskohlii is an endangered multipurpose medicinal plant that has widespread applications. In spite of this, there have been few attempts to propagate its cultivation in India. The present communication presents an in vitro rapid regeneration method using leaf explants of Coleus forskohlii through direct organogenesis. Leaf explants that wer...

A simple protocol was developed for plantlet regeneration through organogenesis from leaf segments of Jatropha curcasL. Ten-day-old seedlings aseptically germinated on sand wet with water were used leaf and hypocotyl explant source. Initial incubation for 5 days in dark at 26 ± 2°C followed by transfer to 16/8-h light/dark cycle which lead to high...

A reproducible protocol for establishment of hairy root cultures of Psoralea corylifolia L. was developed using Agrobacterium rhizogenes strain ATCC 15834. The hairy root clones exhibited typical sigmoid growth curves. Genomic and metabolomic profiles of hairy root clones along with that of untransformed control were analysed. Hairy root clones, Ps...

Collection season of explants from field-grown Mussaenda phillipica (variety: pink) plant have been found to influence initiation of callus growth and shoot bud maturation due to variation in releasing growth- inhibitory phenolic compounds and/or their oxidation products in medium. Release of these chemicals was maximum during May-July and was mini...

Photoautotrophic culture of Chrysanthemum was established under CO2 enrichment (2% v/v). The shoot length and number of leaves were almost equal (2.7 cm and 14 respectively) both under photoautotrophic and photomixotrophic cultures, recorded after four weeks of incubation. Similarly, average dry mass of the plantlets were comparable (31 and 28 mg r...

The efficacies of sago (from Metroxylon sagu Rottb.) and isubgol (from Plantago ovata Forsk.) as gelling agents and those of filter paper, nylon cloth, polystyrene foam and glass wool cloth as support matrices have been tested for the propagation of plantlets of chrysanthemum (Dendranthema grandiflora Tzvelev). The performances of these low-cost ge...

A procedure for rapid multiplication of Chrysanthemum morifolium RAMAT cv. Birbal Sahni using leaf callus and stem (nodal/internodal) callus as well as node and apical shoots has been developed. Murashige and Skoog's medium (1962) supplemented with 2mg/1 2,4-D yielded good green calli from both leaf and stem segments within 2 weeks. About 1 cm × 1...