Parin Chaivisuthangkura

Parin Chaivisuthangkura
Srinakharinwirot University · Department of Biology

Ph.D.

About

98
Publications
21,536
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1,649
Citations
Citations since 2017
19 Research Items
721 Citations
2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140
Additional affiliations
January 1999 - present
Srinakharinwirot University
Education
June 1992 - November 1998
University of Connecticut
Field of study
  • Molecular Biology

Publications

Publications (98)
Article
Full-text available
Vibrio alginolyticus is a major bacterial pathogen causing disease in marine animals. The present study aimed to develop a loop-mediated isothermal amplification (LAMP) coupled with a lateral flow dipstick (LFD) for rapid and simple visual detection of V. alginolyticus–specific amplicons. The biotin-labeled LAMP amplicons from the targeted portion...
Article
Full-text available
Aeromonas caviae is a bacterial pathogen that causes various infectious diseases in both humans and animals. To facilitate its detection, we developed species-specific primer sets targeting polymorphisms in the gyrB gene for use in a PCR assay. The technique was able to detect 100% (29/29) of the A. caviae strains tested using either of two sets of...
Article
Apoptosis is an essential immune response to protect invertebrates from virus infected cells. In shrimp, virus infection has been reported to induce apoptosis. Macrobrachium rosenbergii (Mr) was considered to be a disease-resistant host when compared to penaeid shrimps. Caspase-3 was classified as an executioner caspase which played a key role in v...
Article
Full-text available
Among shrimp viral pathogens, white spot syndrome virus (WSSV) and yellow head virus (YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus (Litopenaeus) vannamei, and the black tiger shrimp, Penaeus (Penaeus) monodon. Another important virus that infects P. vannamei is infectious myonecrosis virus (IMNV),...
Article
Objective: Hemocyanin is a copper-bearing protein in the hemolymph of many arthropods and mollusks and functions as an oxygen transport and important nonspecific immune protein. Methods: In this study, complementary DNA of hemocyanin isoform 2 of the prawn Macrobrachium rosenbergii (MrHc2) was isolated by rapid amplification of cDNA ends and mRN...
Article
Infectious spleen and kidney necrosis virus (ISKNV) is one of the detrimental viruses causing economic losses in several fish species including Asian seabass (Lates calcarifer). In this study, monoclonal antibodies specific to major capsid protein (MCP) of ISKNV were generated. The MCP encoding gene of ISKNV genotype II was cloned into pGEX-6P-1 an...
Article
Preparations of outer membrane proteins (OMPs) obtained from pathogenic Flavobacterium columnare were used as antigens to produce monoclonal antibodies (MAbs). Five MAbs bound to different antigens were selected, four of which bound to only F. columnare, and one demonstrated cross-reactivity with a closely related species F. indicum. Immunohistoche...
Article
Scale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (Lates calcarifer). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil-DNA glycosylase (UDG)-supplemented cross-priming amplification (UCP...
Article
Full-text available
Translationally controlled tumor protein (TCTP) or fortilin is a highly conserved protein among eukaryotes and has important biological roles including anti-apoptotic activity which involves the elimination of virus-infected cells. In this study, the full-length cDNA of Macrobrachium rosenbergii TCTP (MrTCTP) was isolated. The cDNA sequence consist...
Article
A nanogold‐based immunochromatographic strip test (VCG strip test) for the detection of all 70 isolates of Vibrio cholerae including O1, O139, and non‐O1/O139 (NVCs) was developed using two monoclonal antibodies (MAbs), namely VC‐63 and VC‐201, which bound specifically to 10 and 15 kDa proteins. Direct detection of V. cholerae in experimentally spi...
Article
Two monoclonal antibodies (mAbs) specific to Salmonella were produced from a mouse immunized with a combination of heat‐killed whole‐cell and formalin‐fixed Salmonella enterica serovar Enteritidis (S. Enteritidis) as the antigens. The mAb SE‐13D bound specifically only to S. Enteritidis, while mAb Sal‐06G bound to various members of the Salmonella...
Article
Megalocytivirus ISKNV and nervous necrosis virus (NNV) are two major viral pathogens affecting marine fish farms in Asia Pacific region. The present study reports an unexpected discovery of dual infections of both viruses in a single Asian sea bass (Lates calcarifer) farm experiencing ~50% cumulative fish mortality. A commercial Grunt Fin (GF) cell...
Article
Here, two monoclonal antibodies (MAbs) specific to different epitopes on ToxB, a toxin produced by Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VPAHPND), were employed to develop a rapid strip test. One MAb was conjugated to colloidal gold to bind to ToxB at the application pad, and another MAb was used to capture co...
Article
Full-text available
Background: Macrobrachium rosenbergii, is one of a major freshwater prawn species cultured in Southeast Asia. White tail disease (WTD), caused by Macrobrachium rosenbergii nodavirus (MrNV), is a serious problem in farm cultivation and is responsible for up to 100% mortality in the post larvae stage. Molecular data on how M. rosenbergii post-larvae...
Article
Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes...
Article
Full-text available
The simple immunoprecipitation method was used to isolate tilapia immunoglobulin (Ig) for immunization in order to produce monoclonal antibodies (MAbs) specific to tilapia Ig. First, the tilapia antiserum against bovine serum albumin (BSA) was prepared by peritoneal injection of BSA into tilapia, and the tilapia anti‐BSA antiserum was used to preci...
Article
Spätzle protein is an extracellular ligand of Toll receptor in Toll signaling pathway involved in the embryonic dorsoventral patterning and in the innate immunity. In this study, a spätzle gene of freshwater prawn, Macrobrachium rosenbergii (MrSpz) was isolated and characterized. The open reading frame of MrSpz consisted of 747 nucleotides encoding...
Article
Toxins PirvpA and PirvpB were first described in 2014 from Vibrio parahaemolyticus (VP) isolates discovered in 2013 as the cause of acute hepatopancreatic necrosis disease (AHPND) that emerged as a new disease of Chinese cultivated shrimp in 2009. It was subsequently reported from Thailand in 2012. By using PirvpA and PirvpB specific monoclonal ant...
Article
Full-text available
Pseudomonas aeruginosa (P. aeruginosa) is an important opportunistic pathogen that causes serious infections in humans, including keratitis in contact lens wearers. Therefore, establishing a rapid, specific and sensitive method for the identification of P. aeruginosa is imperative. In the present study, the uracil-DNA-glycosylase-supplemented loop-...
Article
Haemocyanin is a multi-subunit protein complex found in the haemolymph and is involved in the immune system of crustaceans. In this study, a haemocyanin gene of Macrobrachium rosenbergii, designated MrHc, was successfully isolated. The MrHc gene contained an open reading frame (ORF) of 1,992 nucleotides, encoding a protein of 663 amino acid residue...
Article
Full-text available
Acute hepatopancreatic necrosis disease (AHPND) of shrimp is caused by Vibrio parahaemolyticus isolates (VPAHPND isolates) that harbor a pVA plasmid encoding toxins PirAVp and PirBVp. These are released from VPAHPND isolates that colonize the shrimp stomach and produce pathognomonic AHPND lesions (massive sloughing of hepatopancreatic tubule epithe...
Article
Toxin A (ToxA) and toxin B (ToxB) of Vibrio parahaemolyticus, which cause acute hepatopancreatic necrosis disease (AHPND), were prepared in a bacterial supernatant from Chinese isolates (CN-VPAHPND) by washing bacterial colonies off of TSA cultures. The supernatant was subsequently used in mouse immunization to produce monoclonal antibodies (MAbs)....
Article
Two monoclonal antibodies (MAbs) specific to different epitopes on ICP11, a non-structural protein of white spot syndrome virus (WSSV), were employed to develop a highly sensitive immunochromatographic strip test (ICP11 strip test). One MAb (WI-11) was conjugated to colloidal gold to bind to ICP11 and another MAb (WI-16) was used to capture colloid...
Article
Full-text available
Loop-mediated isothermal amplification (LAMP) is a rapid, specific, and effective DNA amplification assay. In this study, a duplex LAMP (dLAMP) assay combined with chromatographic lateral flow dipstick (LFD) was developed for the simultaneous identification of Vibrio vulnificus and V. parahaemolyticus. Each primer set that comprised F3, B3, FIP, an...
Article
A portion of the VP26 gene (VP26F109) encoding a structural protein of white spot syndrome virus was expressed, purified by SDS-PAGE and used for immunization of Swiss mice for monoclonal antibody (MAb) production. Three groups of MAbs specific to different epitopes on VP26 were selected; these MAbs can be used to detect natural WSSV infection in P...
Article
Context: A comparatively small number of species, e.g., Vibrio parahaemolyticus and V. vulnificus, cause disease in both aquatic animals and humans. V. harveyi is marine animal pathogen and rarely causes infections in humans; however, it might become a reservoir of antibiotic-resistant bacteria forms and virulence genes. Aims: 1) to develop rapid m...
Article
Full-text available
Vitellogenesis in crustaceans is under the inhibitory regulation of the endocrine system in the X-organ/sinus gland complex in the eyestalks. In this study, two monoclonal antibodies specific to Macrobrachium rosenbergii vitellin and vitellogenin were generated to assess vitellogenesis-inhibiting hormone (VIH) activity. A combination of two monoclo...
Article
Full-text available
A strip test was developed for detection of infectious myonecrosis virus (IMNV) using a pair of monoclonal antibodies (MAbs), called IMN7 and IMC6, that are specific for the N and C fragments, respectively, of the IMNV capsid protein. The test strips were placed in plastic cassettes and stored desiccated in sealed plastic bags. In detection assays...
Article
Full-text available
The aim of this study was based on experimental infection via the pathogenic injections and natural infections by Yellow-head Virus (YHV). Whiteleg shrimp (Litopenaeusvannamei) were experimentally infected with YHV by either injection or co-habitation with YHV-injected shrimp. Thereafter, YHV load, L. vannamei Toll (LvToll) mRNA and LvToll protein...
Article
Full-text available
The icp11 gene encoding the highly abundant DNA mimic protein of white spot syndrome virus (WSSV) was cloned into the pTYB1 and pGEX-6P-1 expression vectors and introduced into E. coli by transformation. After induction, C-terminally intein-tagged ICP11 (ICP11-intein) and N-terminally glutathione-S-transferase (GST)-tagged ICP11 (GST-ICP11) protein...
Article
Full-text available
Monoclonal antibodies (MAbs) specific to Vibrio parahaemolyticus were successfully generated. According to the specificity of V. parahaemolyticus, MAbs can be classified into 5 groups. The MAbs VP-2D and VP-11H were specific to the O2 and O4 groups of V. parahaemolyticus, respectively. The MAb VP-11B reacted with 11 out of 30 isolates of V. parahae...
Article
A strip test for the detection of Vibrio cholerae O139 was developed using two monoclonal antibodies (MAbs), namely VC-273 and VC-812, which specifically bind to the lipopolysaccharide and capsular polysaccharide of V. cholerae O139. The MAb VC-273 gold nanoparticle conjugate was sprayed onto a glass fiber pad that was placed adjacent to a sample c...
Article
Full-text available
vp1, a gene encoding one of the capsid proteins of Taura syndrome virus, was cloned into the pGEX-6P-1 expression vector, and the resulting construct was then used to transform E. coli strain BL21. After induction, an N-terminally glutathione-S-transferase-tagged VP1 (GST-VP1) protein with a molecular mass of 80 kDa was obtained. This protein was p...
Article
Full-text available
The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S-transferase (GST)-fusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal a...
Article
An immunochromatographic strip test is described for detection of the polyhedrin protein of Penaeus monodon nucleopolyhedrovirus (PemoNPV). The test employs one monoclonal antibody (MAb MBV5) conjugated to colloidal gold to bind to polyhedrin protein and a 1:1:1 mixture of 3 other MAbs (MBV8, 14 and 21) to capture colloidal-gold MAb-protein complex...
Article
The gene encoding the capsid protein of Macrobrachium rosenbergii nodavirus (MrNV) was cloned into pGEX-6P-1 expression vector and then transformed into the Escherichia coli strain BL21. After induction, capsid protein-glutathione-S-transferase (GST-MrNV; 64 kDa) was produced. The recombinant protein was separated using SDS-PAGE, excised from the g...
Article
Two segments of the gene encoding the polyhedrin protein of Penaeus monodon nucleopolyhedrovirus (PemoNPV) were cloned into the pTYB1 (759bp) and pGEX-6P-1 (614bp) expression vectors and then transformed into the BL21 Escherichia coli strain. After induction, a fusion of the OB-N-intein (OB-N-intein; 83.2kDa) and OB-C glutathione-S-transferase (GST...
Article
Seven different monoclonal antibodies (MAbs) specific to only Vibrio cholerae were produced using a combination of five representative serotypes of V. cholerae for immunization. The first three MAbs (VC-93, VC-82 and VC-223) were specific to the V. cholerae serogroup O1 with different avidity for the serotypes O1 Inaba and O1 Ogawa. The fourth and...
Article
A novel loop-mediated isothermal amplification (LAMP) combined with amplicon detection by chro-matographic lateral flow dipstick (LFD) assay was developed and evaluated for the detection of Vibrio vulnificus. Biotinylated LAMP amplicons were produced by a set of six designed primers that recognized the V. vulnificus RNA polymerase subunit sigma fac...
Article
A novel loop-mediated isothermal amplification (LAMP) combined with amplicon detection by chromatographic lateral flow dipstick (LFD) assay was developed and evaluated for the detection of Vibrio vulnificus. Biotinylated LAMP amplicons were produced by a set of six designed primers that recognized the V. vulnificus RNA polymerase subunit sigma fact...
Article
The current study was aimed to develop a loop-mediated isothermal amplification (LAMP) combined with amplicon detection by chromatographic lateral flow dipstick (LFD) assay for rapid and specific detection of Vibrio parahaemolyticus. Biotinylated LAMP amplicons were produced by a set of four designed primers that recognized specifically the V. para...
Article
A strip test for the dual detection of white spot syndrome virus (WSSV) and yellow head virus (YHV) was developed using monoclonal antibodies (MAbs) specific to the WSSV major envelope protein VP28 (W1 and W30) and the YHV nucleocapsid protein p20 (Y19 and Y21). The MAbs W30 and Y19 were conjugated with colloidal gold and sprayed onto a glass fiber...
Article
Penaeus monodon densovirus (PmDNV), also called hepatopancreatic parvovirus (HPV), is a pathogen that causes stunt disease in the tiger shrimp, Penaeus monodon. In this study, the gene sequence encoding the capsid protein of PmDNV was cloned into two parts: PmDNV-N (837 bp) and PmDNV-C (806 bp). Both PCR products were cloned into the pGEX-6P-1 expr...
Article
A combination of eight isolates of Aeromonas hydrophila was used to produce monoclonal antibodies (MAbs). Ten different groups of MAbs specific to Aeromonas were selected. The first five groups of MAbs demonstrated high specificity and bound to only one or two isolates of A. hydrophila. The sixth and the seventh groups of MAbs were A. hydrophila sp...
Article
The gene encoding the capsid protein in ORF1 of the genome of infectious myonecrosis virus (IMNV) (GenBank AY570982) was amplified into three parts named CP-N (nucleotides 2248-3045), CP-I (nucleotides 3046-3954) and CP-C (nucleotides 3955-4953). The CP-N fragment was inserted into expression vector pTYB1 while CP-I and CP-C were each inserted into...
Article
The gene encoding the VP19 envelope protein of white spot syndrome virus (WSSV) was cloned into pMAL-C2 expression vector and transformed into the BL21 Escherichia coli strain. After induction, recombinant maltose binding protein (MBP)-VP19 fusion protein was produced, purified and electroeluted before use for immunization in Swiss mice for monoclo...
Article
Vibrio mimicus, a species closely related to V. cholerae, is a type of pathogenic bacteria that causes gastroenteritis in humans and has been reported as a pathogen in shrimp farming. To facilitate the diagnosis of V. mimicus infection, monoclonal antibodies (MAb) against V. mimicus were generated using a combination of four isolates of V. mimicus...
Article
Field specimens of post-larvae of the giant freshwater prawn (Macrobrachium rosenbergii) from Thailand showed hepatopancreatic tubule epithelial cells that contained central, eosinophilic inclusions within enlarged nuclei and marginated chromatin. These inclusions resembled those produced by some baculoviruses prior to formation of occlusion bodies...
Article
Taura syndrome virus (TSV) is one of the major pathogens causing mortality in the whiteleg shrimp, Litopenaeus vannamei. In this study, the gene sequence encoding the VP2 capsid protein (40 kDa) of TSV was cloned into pMAL-C2 expression vector. Five monoclonal antibodies (MAbs) were produced against the VP2 capsid protein, which was expressed heter...
Article
Penaeus stylirostris densovirus (PstDNV), called formerly infectious hypodermal and hematopoietic necrosis virus (IHHNV), is an important shrimp pathogen which can cause mortality in the blue shrimp Penaeus (Litopenaeus) stylirostris and stunting in the whiteleg shrimp Penaeus (Litopenaeus) vannamei. Five monoclonal antibodies (MAbs) were produced...
Article
Loop-mediated isothermal amplification (LAMP) is a novel, sensitive and rapid method for amplification of nucleic acids under isothermal conditions. In this report, a LAMP method was developed for detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV), known previously as monodon baculovirus (MBV), using a set of six primers designed to specif...
Article
The present study was aimed to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Vibrio cholerae. A set of five designed primers that recognized specifically the V. cholerae ompW gene was used. The optimized time and temperature conditions for the LAMP assay were 75 min at 65 degrees C, respectively....
Article
Full-text available
A warm-temperature-adapted breeding pool of Wisconsin Fast Plants (rapid-cycling Brassica rapa; RBr) was developed as a biologically instructional material. Experimentation with the warmtemperature-adapted RBr was used to study students' investigative skills, understanding of the nature of science, and knowledge of the life cycle of flowering plant...
Article
The full-length of the occlusion body (OB) protein gene of Penaeus monodon nucleopolyhedrovirus (PemoNPV) was successfully isolated. The OB gene sequence contained an open reading frame (ORF) of 1359 nucleotides encoding a protein of 452 amino acid residues with a predicted molecular mass of 50.6 kDa. A putative late promoter element, TAAG, was ide...
Article
In the present study, multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for simultaneously detection of six major shrimp viruses including yellow-head virus (YHV), white spot syndrome virus (WSSV), Taura syndrome virus (TSV), hepatopancreatic parvovirus (HPV), infectious hypodermal and hematopoietic necrosis virus (I...
Article
Immunohistochemical study using monoclonal antibodies specific to various shrimp viruses and Vibrio spp. was performed in shrimp samples died from unknown cause with symptoms of black stripes on lateral sides of cephalothorax or smoky body coloration. The positive results in muscular tissue were obtained with MAb VAL57 (specific to Vibrio spp.) and...
Article
Full-text available
The DNA sequence that encodes the first 406 amino acid residues at the N-terminus of yellow head virus (YHV) protein gp116, namely N/2 gp116deltaTM, and the DNA sequence that encodes the next 392 amino acid residues at the C-terminus of gp116 (without the transmembrane region), namely C/2 gp116deltaTM, were cloned into pGEX-6P-1 plasmid and express...
Article
Full-text available
The gene sequence encoding VP3 capsid protein of Taura syndrome virus (TSV) was cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant GST-VP3 (rVP3) fusion protein was obtained and further purified by electro-elution before use in immunizing Swiss mice for production of monoclonal antibodie...
Article
Mouse monoclonal antibodies (MAbs) and rabbit polyclonal antibody (PAb) against Vibrio harveyi were generated from immunization of mice and rabbits with highly virulent isolate of V harveyi. Two MAbs specific to virulent isolates of V harveyi were obtained and one of them (VH4) was selected to conjugate with colloidal gold as the detector antibody...
Article
Full-text available
Whole cell of Aeromonas hydrophila 1234 was used for immunization to produce monoclonal antibodies (MAbs). Three different groups of MAbs specific to Aeromonas were obtained. The first group of MAbs demonstrated high specificity and bound to the A. hydrophila 1234 only but did not bind to the other two A. hydrophila isolates. This group of MAbs bou...
Article
A simple yellow head virus (YHV) "strip test" was developed using monoclonal antibody Y19 (against the p20 structural protein) conjugated with colloidal gold as the detector antibody. Rabbit anti-recombinant p20 (rp20) protein antibody was used as a capture antibody at the test line (T) and goat anti-mouse IgG antibody (GAM) was used as the capture...
Article
Full-text available
A simple strip-test kit for white spot syndrome virus (WSSV) detection was developed using monoclonal antibody W29 (against the VP28 structural protein) conjugated with colloidal gold as the detector antibody. A rabbit anti-recombinant VP28F118 (rVP28) protein antibody in combination with a W28 monoclonal antibody was used as the capture complex at...