Paolo Bonini

• CEO at oloBion

Unidentified peaks remain a major problem in untargeted metabolomics by LC-MS/MS. Confidence in peak annotations increases by combining MS/MS matching and retention time. We here show how retention times can be predicted from molecular structures. Two large, publicly available datasets were used for model training in machine learning: the Fiehn hyd...

Widespread drought conditions have increasingly affected agricultural productivity, requiring the exploration of alternative approaches for improving crop resilience, yield and quality, since plants adopt many physiological strategies to cope with challenging environments. This study evaluated the effects of a vegetal-derived protein hydrolysate (P...

Introduction High-throughput phenotyping technologies together with metabolomics analysis can speed up the development of highly efficient and effective biostimulants for enhancing crop tolerance to drought stress. The aim of this study was to examine the morphophysiological and metabolic changes in tomato plants foliarly treated with two protein h...

The demand for high-quality strawberries continues to grow, emphasizing the need for innovative agricultural practices to enhance both yield and fruit quality. In this context, the utilization of natural products, such as biostimulants, has emerged as a promising avenue for improving strawberry production while aligning with sustainable and eco-fri...

In response to tackling the environmental consequences of fertiliser production, biofertilisers from organic sources are strongly promoted in line with circular economy and maximising resource use. Despite the outstanding potential of bio-based fertilisers for the sustainable development of the agricultural sector, an environmental investigation of...

Introduction The fermentative production of auxin/indole 3-acetate (IAA) using selected Pantoea agglomerans strains can be a promising approach to developing novel plant biostimulants for agriculture use. Methods By integrating metabolomics and fermentation technologies, this study aimed to define the optimal culture conditions to obtain auxin/IAA...

The application of microbial biostimulants to plants has revealed positive effects related to nutrients uptake, stress tolerance, root development and phenological growth. However, little information is available exploiting the potential synergistic biostimulant action of microbes on the functional quality of the yields. The current research elucid...

Intensive greenhouse vegetable production is often associated with a decline of crop productivity due to the increase of soil salinity and/or a reduction of biological fertility. The aim of the current work was to assess the effects of three organic fertilizers on morpho-physiological and agronomic traits of greenhouse lettuce as well as soil enzym...

Co-inoculation of arbuscular mycorrhizal fungi (AMF) and bacteria can synergically and potentially increase nitrogen use efficiency (NUE) in plants, thus, reducing nitrogen (N) fertilizers use and their environmental impact. However, limited research is available on AMF-bacteria interaction, and the definition of synergisms or antagonistic effects...

Seed inoculation with beneficial microorganisms has gained importance as it has been proven to show biostimulant activity in plants, especially in terms of abiotic/biotic stress tolerance and plant growth promotion, representing a sustainable way to ensure yield stability under low input sustainable agriculture. Nevertheless, limited knowledge is a...

In this work, the impact of terroir, cultivar, seasonality, and farming systems on functional traits of tomato was hierarchically investigated. Untargeted metabolomics, antioxidant capacity, colorimetric assays, and enzyme inhibition were determined. The total phenolic and carotenoid contents significantly varied between growing years, whereas an i...

I risultati delle prove sperimentali riportati in questo articolo evidenziano come i trattamenti al seme delle orticole con sostanze e microrganismi ad azione biostimolante siano efficaci nel migliorare la germinabilità, l’uniformità di germinazione e il vigore delle piante, a tutto vantaggio di resa e qualità del prodotto finale

Bioactive compounds produced by plant growth-promoting bacteria through a fermentation process can be valuable for developing innovative second-generation plant biostimulants. The purpose of this study is to investigate the biotechnological potential of Enterobacter on the production of auxin—a hormone with multiple roles in plant growth and develo...

In this study, an untargeted metabolomics approach based on ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) was used for investigating changes in chemical profiles of cow milk considering diets based on mycotoxins-contaminated corn silages. For this purpose, 45 milk samples were classified in...

Salinity in water and soil is one of the major environmental factors limiting the productivity of agronomic and horticultural crops. In basil (Ocimum basilicum L., Lamiaceae) and other Ocimum species, information on the plant response to mild salinity levels, often induced by the irrigation or fertigation systems, is scarce. In the present work, we...

Natural biostimulants are one of the most promising and eco-sustainable technologies, which can improve the qualitative and productive attributes of horticultural crops. The positive effects of their application derive from their “signaling role” as elicitors of auxin- and gibberellin-like activity, thus constituting a valid alternative to syntheti...

Sweet cherry is a high value crop and the economic success of its cultivation depends not only on yield but also on fruit visual and nutritional quality attributes that influence consumer acceptability, as well as on fruit post-harvest performance and resistance to cracking. During the last few decades, cherry growers have tried to achieve these go...

Nel nuovo Regolamento euro-peo sui fertilizzanti approva-to il 27 marzo 2019 dal Parla-mento europeo i «biostimo-lanti delle piante» sono definiti come qualunque prodotto che stimola i pro-cessi nutrizionali delle piante indipen-dentemente dal suo tenore di nutrienti, con l'unica finalità di migliorare una o più delle seguenti caratteristiche del-l...

Microbial-based biostimulants can improve crop productivity by modulating cell metabolic pathways including hormonal balance. However, little is known about the microbial-mediated molecular changes causing yield increase. The present study elucidates the metabolomic modulation occurring in pepper (Capsicum annuum L.) leaves at the vegetative and re...

We present Mass Spectrometry-Data Independent Analysis software version 4 (MS-DIAL 4), a comprehensive lipidome atlas with retention time, collision cross-section and tandem mass spectrometry information. We formulated mass spectral fragmentations of lipids across 117 lipid subclasses and included ion mobility tandem mass spectrometry. Using human,...

Plant biostimulants, including microbial and non-microbial based products, have been successfully used to improve agriculture productivity due to their ability to stimulate nutrient uptake, plant growth and to improve tolerance to abiotic stresses. An open field experiment was carried out on processing tomato (Solanum lycopersicum L.) with the aim...

The species Pantoea agglomerans includes strains that are agronomically relevant for their growth-promoting or biocontrol traits. Molecular analysis demonstrated that the IPDC pathway involved in the conversion of tryptophan (Trp) to indole-3-acetic acid (IAA) is highly conserved among P. agglomerans strains at both gene and protein levels. Results...

The species Pantoea agglomerans includes strains that are agronomically relevant for their growth-promoting or biocontrol traits. Molecular analysis demonstrated that the IPDC pathway involved in the conversion of tryptophan (Trp) to indole-3-acetic acid (IAA) is highly conserved among P. agglomerans strains at both gene and protein levels. Results...

A greenhouse experiment was established with loquat plants to investigate the role of arbuscular mycorrhizal fungi (AMF) in the control of the white root rot fungus Armillaria mellea and to determine the changes produced in the plant metabolome. Plants inoculated with two AMF, Rhizoglomus irregulare and a native AMF isolate from loquat soils, were...

Microbial-based biostimulants can improve crop productivity by modulating cell metabolic pathways including hormonal bal-ance. However, little is known about the microbial-mediated molecular changes causing yield increase. The present studyelucidates the metabolomic modulation occurring in pepper (Capsicum annuum L.) leaves at the vegetative and re...

Microbial and non-microbial plant biostimulants have been successfully used to improve agriculture productivity in a more sustainable manner. Since the mode of action of biostimulants is still largely unknown, the present work aimed at elucidating the morpho-physiological and metabolomic changes occurring in maize (Zea mays L.) leaves and roots fol...

To the Editor We formulated mass spectral fragmentations of lipids across 117 lipid subclasses and included ion mobility tandem mass spectrometry (MS/MS) to provide a comprehensive lipidome atlas with retention time, collision cross section, and MS/MS information. The all-in-one solution from import of raw MS data to export of a common output forma...

L' orticoltura è il principale comparto agricolo in cui l'elevata reddittività delle colture induce a utilizzare i biostimolanti per migliorare la pro-duttività, la sostenibilità dei processi produttivi e la qualità del prodotto. Nelle produzioni orticole sia, in pieno campo sia in coltura protetta, l'apporto dei biostimolanti è finalizzato al ragg...

Modulation of the water availability for the plants can be a sustainable option to reduce agricultural water demand and stimulate the synthesis and accumulation of secondary metabolites. However, information on the reduction of water availability while maintaining yield and functional quality are scarce and fragmentary. Accordingly , this research...

Scientific investigations are being increasingly devoted to biostimulant effects on vegetable yield and quality, with the perspective of sustainable crop management. Two farming systems (conventional or organic) in factorial combination with two biostimulant treatments (tropical plant extract (PE); legume-derived protein hydrolysate (PH)) plus a no...

Best Paper Award 2018; Agronomy MDPI

The emerging role of plant biostimulants in enhancing nutrient efficiency is important for maintaining soil fertility under sub-optimal nutrient regimens. We aimed to elucidate the morpho-physiological and biochemical effects as well as mineral composition changes of greenhouse jute (Corchorus olitorius L.) treated with a commercial vegetal-derived...

Plant-derived protein hydrolysates (PHs) are an important category of biostimulants able to increase plant growth and crop yield especially under environmental stress conditions. PHs can be applied as foliar spray or soil drench. Foliar spray is generally applied to achieve a relatively short-term response, whereas soil drench is used when a long-t...

Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective...

The implementation of sustainable agriculture encompasses practices enhancing the activity of beneficial soil microorganisms, able to modulate biogeochemical soil cycles and to affect soil fertility. Among them, arbuscular mycorrhizal fungi (AMF) establish symbioses with the roots of most food crops and play a key role in nutrient uptake and plant...

Root exudation patterns are linked to, among other things, plant growth, plant-microbe interaction and the priming effect. In this work, two complementary metabolomic approaches (both liquid and gas chromatography coupled to mass spectrometry) were applied to investigate the modulation of root exudation imposed by two beneficial fungi (substrate tr...

Plant-derived protein hydrolysates (PHs) have received increased attention in the last decade because of their potential to improve yield, nutritional quality as well as tolerance to abiotic stressors. The current study investigated the effects and the molecular mechanisms of a legume-derived PH under optimal and sub-optimal nitrogen (N) concentrat...

Plant biostimulants (PBs) such as protein hydrolysates and seaweed extracts are attracting the increasing interest of scientists and vegetable growers for their potential toenhance yield and nutritional quality. The current study assessed crop productivity, leaf colorimetry, mineral profile and bioactive compounds of greenhouse spinach in response...

Plant biostimulants are receiving great interest for boosting root growth during the first phenological stages of vegetable crops. The present study aimed at elucidating the morphological, physiological, and metabolomic changes occurring in greenhouse melon treated with the biopolymer-based biostimulant Quik-link, containing lateral root promoting...

Hotelling’s T2 investigation on outliers as carried out in UHPLC/QTOF-MS metabolomic investigations on root and leaf metabolic profile following root application of the biopolymer-based biostimulant.

Visual representation of the VIP-selected discriminating compounds from the OPLS-DA modeling on roots (A) and leaves (B) metabolic profile, following root application of the biopolymer-based biostimulant.

Whole dataset gained from UHPLC/QTOF-MS metabolomic investigations on melon metabolic profile following root application of the biopolymer-based biostimulant. Compounds abundance, together with retention time and composite spectra (mass-abundance pairwise) are provided.

VIP-selected discriminating compounds from OPLS-DA modeling on root and leaf metabolic profile, following root application of the biopolymer-based biostimulant.

Plant-derived protein hydrolysates (PHs) have gained prominence as plant biostimulants because of their potential to increase the germination, productivity and quality of a wide range of horticultural and agronomic crops. Application of PHs can also alleviate the negative effects of abiotic plant stress due to salinity, drought and heavy metals. Re...

The current research aimed 1) at evaluating the effects of three biostimulants (legume-derived protein hydrolysate, PH; plant and seaweed extract, PE and SWE) on yield performance and nutritional quality, mineral profiling, antioxidant activities, lycopene, total phenols and ascorbic acid of greenhouse tomato (Solanum lycopersicum L.) under soil cu...

In the coming years, farmers will have to deal with growing crops under suboptimal conditions dictated by global climate changes. The application of plant biostimulants such as beneficial microorganisms and plant-derived protein hydrolysates (PHs) may represent an interesting approach for increasing crop tolerance to alkalinity and salinity. The cu...

The aim of this study was to assess whether seed coating with microbial consortium based on the arbuscular mycorrhizal (AM) fungus Glomus intraradices BEG72, Glomus mossae and Trichoderma atroviride MUCL 45632 could improve seedling establishment, yield and grain quality (protein content and mineral composition) of wheat (Triticum durum Desf.). As...