Paola Irato

Paola Irato
University of Padova | UNIPD · Department of Biology

PhD

About

118
Publications
16,307
Reads
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2,097
Citations
Citations since 2017
59 Research Items
1043 Citations
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2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300
Introduction
Paola Irato currently works at the Department of Biology, University of Padova. Paola does research in Physiology. Their most recent publication is 'CITRUS FRUITS. COURSE TO EDUCATE TO SCIENTIFIC CURIOSITY'.
Additional affiliations
August 1991 - present
University of Padova
Position
  • Researcher

Publications

Publications (118)
Preprint
Antarctica is the continent with the lowest local human impact, yet it is still vulnerable to contaminants coming from external sources. Emerging pollutants, like PFAS, pose an increasing threat to this environment and therefore require more in-depth investigations to understand their environmental fate and biological impacts. The present study, pa...
Article
Full-text available
Metallothioneins (MTs) are low-molecular weight sulfur-rich proteins, widely distributed in nature. They play a homeostatic role in the control and detoxification of metal ions. Previous studies indicated that MTs also have the capacity to scavenge reactive oxygen species. This study aimed to investigate the evolution of the protein in the notothen...
Article
Full-text available
Metal bioaccumulation and metallothionein (MT) expression were investigated in the gills and liver of the red-blooded Antarctic teleost Trematomus hansoni to evaluate the possibility for this species to face, with adequate physiological responses, an increase of copper and cadmium concentrations in its tissues. Specimens of this Antarctic fish were...
Article
Full-text available
This work aimed to evaluate the effects of zinc (Zn) relating to cadmium (Cd)-induced toxicity and the role played by MTF-1. This transcription factor regulates the expression of genes encoding metallothioneins (MTs), some Zn transporters and the heavy chain of γ-glutamylcysteine synthetase. For this reason, two cell lines of mouse fibroblasts were...
Preprint
Metal bioaccumulation and metallothionein (MT) expression were investigated in gills and liver of the red-blooded Antarctic teleost Trematomus hansoni with the aim to evaluate the possibility for this species to face, with adequate physiological responses, an increase of copper or cadmium concentrations in the environment. Specimens of this Antarct...
Preprint
Metal bioaccumulation and metallothionein (MT) expression were investigated in gills and liver of the red-blooded Antarctic teleost Trematomus hansoni with the aim to evaluate the possibility for this species to face, with adequate physiological responses, an increase of copper or cadmium concentrations in the environment. Specimens of this Antarct...
Article
Full-text available
In recent decades, the interest in PFAS has grown exponentially around the world, due to the toxic effects induced by these chemical compounds in humans, as well as in other animals and plants. However, current knowledge related to the antistress responses that organisms can express when exposed to these substances is still insufficient and, theref...
Preprint
In recent decades, the interest in PFAS has grown exponentially around the world, due to the toxic effects induced by these chemical compounds in humans, as well as in other animals and in plants. However, current knowledge related to the antistress responses that organisms can express when exposed to these substances is still insufficient and ther...
Article
Full-text available
It is well known that the excessive production of reactive oxygen species (ROS) can lead to the peroxidation of membrane lipids, glycation/oxidation/nitration of proteins, inactivation of enzymes, DNA mutation and damage, and other alterations in the subcellular components [...]
Article
The exposure to metals is known to generate oxidative stress in living organisms, which can result in the induction of protective antioxidant defences, both enzymatic and non-enzymatic. This work aims to obtain new data on the existing links among several non-enzymatic components of the antioxidant system, that are physiologically related to both m...
Conference Paper
Full-text available
In the last decade, Science Education has played a crucial role in the training of young students, supporting them in understanding the world around us, making them active citizens, responsible and capable of innovation in a knowledge-based society (Recommendation of the European Parliament and of the Council, 18 December 2006). The purpose of the...
Conference Paper
Full-text available
With a view to the active learning, that makes the students protagonist, responsible and aware of the learning process, the present study presents an experimental work designed to demonstrate the usefulness and the validity of the scientific method as the basic element of the teaching of Life Sciences. Scientific training adopts an inductive approa...
Article
Full-text available
Due to their unique properties, perfluorinated substances (PFAS) are widely used in multiple industrial and commercial applications, but they are toxic for animals, humans included. This review presents some available data on the PFAS environmental distribution in the world, and in particular in Europe and in the Veneto region of Italy, where it ha...
Preprint
Full-text available
Due to their unique properties, perfluorinated substances (PFAS) are widely used in multiple industrial and commercial applications, but they are toxic for animals, humans included. This review presents some available data on the PFAS environmental distribution in the world, and in particular in Europe and in Veneto region of Italy, where it has be...
Article
Full-text available
Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role of GPxs in reactive oxygen species detoxification in the unicellular euka...
Preprint
Full-text available
Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role of GPxs in reactive oxygen species detoxification in the unicellular euka...
Article
Full-text available
With a view to the active learning, that makes the students protagonist, responsible and aware of the learning process, the present study presents an experimental work designed to demonstrate the usefulness and the validity of the scientific method as the basic element of the teaching of Life Sciences. Scientific training adopts an inductive approa...
Article
Full-text available
In the last decade, Science Education has played a crucial role in the training of young students, supporting them in understanding the world around us, making them active citizens, responsible and capable of innovation in a knowledge-based society (Recommendation of the European Parliament and of the Council, 18 December 2006). The purpose of the...
Preprint
Full-text available
Exposure to metals is known to generate oxidative stress in living organisms, which are able to respond with the induction of antioxidant defenses, both enzymatic and non-enzymatic. The aim of this work is to study the correlation among several non-enzymatic component of the antioxidant system, that are physiologically related to both metal sequest...
Article
Full-text available
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approac...
Poster
Full-text available
The LIFE project envisages an extensive monitoring program to assess the diffusion and impacts of PFAS in different environmental matrices water, soil, plants in agriculture areas in the three areas The monitoring planned during all project has been carrying out in 10 selected sampling stations, which are representative of the framed area with diff...
Poster
Full-text available
After the discovery of PerFluoro Alkyl Substances (PFAS) pollution of surface, ground and drinking water in a large area of the Veneto region (Northern Italy), mitigation actions have been immediately put in place. In particular, the Department of Health Protection, Food and Veterinary Safety of the Veneto Region, in association with CNR IRSA, ARPA...
Poster
Full-text available
Starting from the lesson learnt after a massive contamination by perfluoroalkyl substances (PFASs) discovered in 2013, an innovative approach to environmental and health emergencies is here suggested. The pollution episode affected a vast area of groundwater recharge in the North-East of Italy (Veneto Region), with direct consequences on surface wa...
Poster
Full-text available
In 2013 a significant episode of PFAS pollution of surface, ground- and drinking water has been discovered in a large area of the Veneto region, in Northern Italy. The most important source of pollution was identified in a fluorochemical plant, sited in an area of groundwater recharge. The Veneto Region immediately put in place mitigation actions t...
Article
Full-text available
Cathelicidins are an important family of antimicrobial peptide effectors of innate immunity in vertebrates. Two members of this group, CATH‐1 and CATH‐2, have been identified and characterized in teleosts (ray‐finned fish). In this study, we investigated the expression of these genes in different tissues of rainbow trout challenged with 4 different...
Conference Paper
Full-text available
This article illustrates a didactic proposal about evolution theory by natural selection. Evolution helps us to understand the history of life. The central idea of biological evolution is that all life on Earth shares a common ancestor. Through the process of descent with modification, the common ancestor of life on Earth gave rise to the fantastic...
Presentation
Characterization of superoxide dismutases in the Antarctic scallop Adamussium colbecki
Article
Metals can directly or indirectly cause an increase in reactive oxygen species (ROS) accumulation in cells, and this may result in programmed cell death. A number of previous studies have shown that zinc (Zn) modulates mitogenic activity via several signalling pathways, such as AKT, mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (N...
Data
Full-text available
Abstract: This review presents an overview of the significance of the use of molecular biomarkers as diagnostic and prognostic tools for marine pollution monitoring. In order to assess the impact of highly persistent pollutants such as polychlorinated biphenyls (PCB), polychlorinated dibenzo–dioxins (PCDD), polychlorinated dibenzo–furans (PCDF), po...
Article
The main characteristic of Antarctic seawater is its low constant temperature and its high concentration of O(2), which can increase the formation rate of reactive oxygen species (ROS), together with a natural occurrence of elevated cadmium and copper levels. In the present paper, we studied the presence of cadmium, copper and zinc, metallothionein...
Article
Full-text available
Trace elements as Cu (copper), Fe (iron), and Zn (zinc) can be toxic when their distribution is not carefully regulated, and also their inappropriate binding may compromise cellular function and homeostatic mechanisms. Metal transcription factor-1 (MTF-1), a multiple Zn finger protein, activates metal response element-driven (reporter) gene express...
Article
In the present study, the interactions between zinc (Zn) and copper (Cu) or iron (Fe) have been examined. Rat hepatoma cell line H4-II-E-C3, fibroblast cell line mutant MT-/-, and wild-type MT+/+ cells treated with ZnSO4 or CuSO4 or FeSO4 or CuSO4+ZnSO4 or ZnSO4+FeSO4 for different times have been employed to study the effect of metallothionein (MT...
Article
Metallothioneins (MTs) are low-molecular weight, metal- and sulphur-rich proteins that are widely distributed in living organisms. These intracellular proteins are thought to be involved in both non-essential heavy metals detoxification, such as cadmium, and the homeostasis of essential trace metals, such as zinc and copper. It has also been demons...
Article
Full-text available
The physiology of iron metabolism in Wilson's disease is largely unknown, and there is a paucity of data on the real presence and progression of iron accumulation. The purpose of this study was to assess the iron metabolism parameters, including hepatic iron concentration, in follow-up liver biopsies and serum, and urinary pro-hepcidin. Twenty-thre...
Article
Copper, zinc and iron are essential metals for different physiological functions, even though their excess can lead to biological damage. This review provides a background of toxicity related to copper, iron and zinc excess, biological mechanisms of their homeostasis and their respective roles in the apoptotic process. The antioxidant action of met...
Article
The aim of the present work was to determine the activities of selected antioxidant enzymes (SOD, Se-GPX, CAT) in two species of bivalves, Scapharca inaequivalvis and Tapes philippinarum, from two sites of the lagoon of Venice that are characterized by different pO(2) (Marghera and Chioggia). The specimens were collected at four times during a 1-ye...
Article
In this study, we have attempted to explore the possible protection afforded by Zn with regard to its antioxidant potential properties in the iron-induced toxicity. Rat hepatoma cell line H4-II-E-C3 was treated with 150 muM ZnSO4, 200 muM FeSO4 or 150 muM ZnSO4+ 200 muM FeSO4 for 24 h. We studied the effect of metallothionein (MT), glutathione and...