About
13
Publications
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Introduction
I completed my undergraduate and MS in Biomedical Engineering at Shanghai Jiao Tong University. During that time, I developed a novel optical method to control cellular calcium signaling. I was admitted as a PhD student of NYUMC Cell Biology Program in 2018 and joined Davoli Lab in 2019. Now I am studying the consequences and weaknesses associated with random aneuploidy, by integrating large-scale multiomics, genome-wide CRISPR screen and isogenic cell models of aneuploidy.
Current institution
Additional affiliations
August 2018 - present
October 2014 - June 2018
Position
- Research Assistant
Description
- Optical imaging and control of cellular calcium signaling.
July 2014 - September 2014
Position
- Intern
Description
- Designed and implemented algorithms for BOLD fMRI data processing and visualization using Matlab and SPM.
Education
August 2018 - May 2023
September 2015 - March 2018
September 2011 - June 2015
Publications
Publications (13)
Ca ²⁺ channels are essential to cell birth, life, and death. They can be externally activated by optogenetic tools, but this requires robust introduction of exogenous optogenetic genes for expression of photosensitive proteins in biological systems. Here we present femtoSOC, a method for direct control of Ca ²⁺ channels solely by ultrafast laser wi...
How cells control gene expression is a fundamental question. The relative contribution of protein-level and RNA-level regulation to this process remains unclear. Here, we perform a proteogenomic analysis of tumors and untransformed cells containing somatic copy number alterations (SCNAs). By revealing how cells regulate RNA and protein abundances o...
Aneuploidy, the presence of chromosome gains or losses, is a hallmark of cancer. Here, we describe KaryoCreate (karyotype CRISPR-engineered aneuploidy technology), a system that enables the generation of chromosome-specific aneuploidies by co-expression of an sgRNA targeting chromosome-specific CENPA-binding ɑ-satellite repeats together with dCas9...
Aneuploidy, characterized by the gain or loss of chromosomes, plays a critical role in both cancer and congenital aneuploidy syndromes. For any aneuploidy, we can distinguish between its general effects and its chromosome-specific effects. General effects refer to the common cellular stresses induced by aneuploidy, such as impaired proliferation, p...
Aneuploidy, the presence of chromosome gains or losses, is a hallmark of cancer and congenital syndromes. Here, we describe KaryoCreate ( Karyo type CR ISPR E ngineered A neuploidy Te chnology), a system that enables generation of chromosome-specific aneuploidies by co-expression of a sgRNA targeting chromosome-specific CENPA-binding ɑ-satellite re...
How cells control gene expression is a fundamental question. The relative contribution of protein-level and transcript-level regulation to this process remains unclear. Here we perform a proteogenomic analysis of tumors and untransformed cells containing somatic copy number alterations (SCNAs). By revealing how cells regulate transcript and protein...
Significance
We report somatic copy-number alterations (SCNAs) that contribute to an immune microenvironment switch during human papillomavirus-negative head and neck cancer (HNSC) development. Specific and nonspecific SCNA levels were examined in a large prospective oral precancer (188 patients) cohort, 2 HNSC (343, 196 patients) cohorts, and 32 c...
Circulating tumor cells (CTCs) play a key role in cancer metastasis but are very difficult to detect. in vivo monitoring CTCs has been recognized as an important technique for cancer research and clinical diagnosis. Recently, a noninvasive method, in vivo flow cytometry (IVFC) has been developed to enable continuous, real‐time, and long‐duration de...
Two-photon microscopy (TPM) has been one of the most widely used fluorescent
microscopy methods in biological tissue. The femtosecond laser in the near-infrared
(NIR) range showed better penetration in tissue due to less scattering and absorption.
The method to estimate the optimal wavelength generally according to the scattering
and absorption is...
Two-photon scanning by femtosecond laser on subcellular microregions can induce long-duration Ca2+ increase. The wavelength-dependent spectrum of Ca2+ activation probability suggests endogenous photoactivable molecules which proved to be flavin. Thereby a new all-optical method for photoactivation of cellular Ca2+ can be developed.
Laser technology has been promoting various microscopy methods and thus making great progresses in life science. Further than contribution to “seeing is believing”, lasers have also demonstrated their capacity of manipulating cells and even molecular signaling. Specifically, with advances of lasers and combination with other techniques, recent repo...
The ability to regulate endogenous gene expression is critical in biological research. Existing technologies, such as RNA interference, zinc-finger regulators, transcription-activator-like effectors, and CRISPR-mediated regulation, though proved to be competent in significantly altering expression levels, do not provide a quantitative adjustment of...
