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Pamela Chavez-Crooker

Pamela Chavez-Crooker
Domolif SpA · Research & Development

Doctor of Molecular Microbiology and Biotechnology

About

22
Publications
8,373
Reads
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752
Citations
Additional affiliations
January 2008 - present
Aguamarina SA
Position
  • Research Director
Description
  • We develop technology (bio) for mining industry in the areas of Biocorrosion, Bioleaching, Dust COntrol and water treatment.
March 1999 - December 2007
University of Antofagasta
Position
  • Professor (Associate)
Education
January 2012 - January 2013
Columbia University
Field of study
  • Business
March 2011 - April 2012
Universidad Adolfo Ibáñez
Field of study
  • Business
April 1998 - January 1999
University of Hawaiʻi at Mānoa
Field of study
  • Fisiology of heavy metal transport in cells

Publications

Publications (22)
Article
Full-text available
The use of micro organisms for heavy metal remediation in water is a technique widely studied. This review describes a number of methods used for acid mine drainage (AMD) remediation, containing high concentration of this type of contaminant. The AMD is a problem generated in abandoned mines and low grade stock of active mines, therefore it is an e...
Article
Full-text available
Background: Mining operations generate high demand of water resources in the areas where they operate; in Chile, the majority of major mining operations are concentrated in the north, close to the Atacama Desert, the driest desert in the world. Fresh water is no longer available in big quantities in the region; for this reason, new alternatives hav...
Article
Full-text available
The mining industry is looking forward for bacterial consortia for economic extraction of copper from low-grade ores. The main objective was to determine an optimal bacterial consortium from several bacterial strains to obtain copper from the leach of chalcopyrite. The major native bacterial species involved in the bioleaching of sulphide ore (Acid...
Article
Numerous studies have investigated the reproduction mechanisms in mollusc species at a biochemical and physiological level; few have described these mechanisms at a molecular level, despite great commercial interest in several mollusc species. We investigated genes involved in gonad maturation of the marine scallop Argopecten purpuratus. A cDNA lib...
Article
Full-text available
This investigation combines confocal microscopy with the cation-specific fluorescent dyes Fluo-3 and BTC-5N to localize calcium and heavy metals along the length of intact lobster (Homarus americanus) hepatopancreatic tubules and isolated cells. A metallothionein-specific antibody, developed in mollusks with cross-reactivity in crustaceans, showed...
Article
This investigation combines confocal microscopy with the cation-specific fluorescent dyes Fluo-3 and BTC-5N to localize calcium and heavy metals along the length of intact lobster (Homarus americanus) hepatopancreatic tubules and isolated cells. A metallothionein-specific antibody, developed in mollusks with cross-reactivity in crustaceans, showed...
Article
Lysosomes are known centers for sequestration of calcium and a variety of heavy metals in many invertebrate tissues, and as a result of this compartmentalization these organelles perform important detoxification roles in the animals involved. The present investigation uses a centrifugation method to isolate and purify hepatopancreatic lysosomes fro...
Article
Full-text available
As never before, biological and medical sciences in Latin America have been caught unprepared with the explosive development of genome biology with its concomitant use of high-throughput technologies, large scale database management, sophisticated instrumentation and collaborative research. In the past, new biological technologies, such as those de...
Article
Mechanisms of copper transport into purified mitochondrial suspensions prepared from the hepatopancreas of the Atlantic lobster Homarus americanus were investigated. Mitochondria were purified by combining methods of differential and Percoll-gradient centrifugation, and copper transport was studied using the copper-sensitive fluorescent dye Phen Gr...
Article
Full-text available
The hepatopancreas of the American lobster (Homarus americanus) possesses four types of epithelial cells arranged along blind-ended tubules. At the distal tips of these tubules, stem cells termed E-cells differentiate into three other cell types, R-cells, F-cells and B-cells, each of which have different absorptive and secretory roles in the biolog...
Article
A novel intracellular serine proteinase from the marine aerobic hyperthermophilic archaeon Aeropyrum pernix K1 (JCM 9820) that we designated pernilase was purified by ammonium sulfate precipitation, anionic-exchange chromatography, affinity chromatography, and gel filtration chromatography. The purified enzyme was composed of a single polypeptide c...
Article
An extracellular metalloproteinase, which we had designated aeropyrolysin, from the aerobic marine hyperthermophilic archaeon Aeropyrum pernix K1 (JCM 9820), was purified by ammonium sulfate precipitation, anionic exchange chromatography, and gel filtration chromatography. The purified enzyme was composed of a single polypeptide chain with a molecu...
Article
Full-text available
The behavioral responses of veliger larvae of the gastropod Concholepas concholepas were studied in the presence of different natural and artificial settlement cues and microbial films. Early pre-competent larvae stopped swimming, sank (due to ciliary arrests, retraction of the velum into the shell, or both), and remained inactive on the substratum...
Article
In this investigation bacteriological analyses of the early developmental stages of Argopecten purpuratus were performed on scallops spawned in situ (hatchery), and in the laboratory under sterile conditions. The results confirmed the occurrence of vertical bacterial transmission. In spawning in the laboratory Pseudomonas, Moraxella and Vibrio were...
Article
Full-text available
A Vibrio anguillarum-related (VAR) strain, isolated in pure culture from an epizootic in a commercial hatchery producing Argopecten purpuratus, was characterized, and its potential pathogenicity to veliger larvae of A. purpuratus determined. Experimental challenges indicated that the bacterium affects larval survival at concentrations of 10(4) to 1...
Article
Full-text available
Argopecten purpuratus is one of the most valuable molluscs in aquaculture activities in Chile. However, there are several problems for mass production of seeds under controlled condition. One of these is bacterial infection in early larval stages. In this study, breeders were analyzed as possible route for bacterial entrance to cultures. Bacteria a...

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Projects

Project (1)
Project
Development of a new technology for solid disposal of mining tailings. 1. Development of a new construction material from tailings. 2. Improvement of water recuperation from tailings.