Palanichamy Esakkiraj

• PhD
• PostDoc Position at Jichi Medical University

In this study, fermented rice gruel, a traditional food in South Telangana, India, was used as a source for the isolation of biologically significant probiotics. A total of 56 morphologically diverse strains were isolated and screened for their ability to inhibit dental biofilms as well as foodborne and aquaculture bacterial pathogens. Among these,...

The Box-Behnken design was applied to determine the optimal parameters of the extraction condition by using the response surface methodology (RSM) from the leaves of Sonneratia caseolaris L. The result indicates the best-optimized conditions used for the extraction of polysaccharides at 84.02 °C temperature, 3.12 h time, and 27.31 mL/g for the wate...

The shrimp immune system defends and protects against infection by its naturally expressing antimicrobial peptides. Stylicin is a proline-rich anionic antimicrobial peptide (AMP) that exhibits potent antimicrobial activity. In this study, stylicin gene was isolated from Penaeus vannamei, cloned into vector pET-28a ( +), and overexpressed in Escheri...

Background Selenium (Se) is considered an important micronutrient for human and animal health having potential biological functions and it is required for the prevention and treatment of diseases. The present study was designed to optimize the synthesis conditions of selenium nanoparticles by the Taguchi design method using sodium selenite as a pre...

A gene encoding lipase enzyme from Bacillus flexus PU2 was cloned and expressed in E. coli BL21 (DE3) pLysS and purified protein having the molecular weight of 34 kDa. This lipase was found to be alkaline (pH 9) and slightly thermophilic. This lipase was observed to retain its activity in the presence of methanol, ethanol, DMSO, and acetone. Ferrou...

Avicennia marina mangrove leaves polysaccharide (AMLP) was used for the synthesis of polysaccharide-based selenium (AMLP-SeNPs) and silver nanoparticles (AMLP-AgNPs). The synthesized nanoparticles were further characterized by UV-Vis, DLS, FT-IR, X-ray diffraction, and HR-TEM analysis. A 60-day (8 weeks) feeding trial experiment was conducted to in...

Experiments were conducted to evaluate the probiotic effect of bio-augmented Bacillus tequilensis AP BFT3 on improving production, immune response, and proteomic changes of Penaeus vannamei reared in a biofloc system. Penaeus vannamei larvae (PL13) were stocked in 100-L tanks at a rate of 100 no per tank to study the effect of B. tequilensis AP BFT...

The lipase gene from Psychrobacter celer PU3 was cloned into pET-28a(+) expression vector and overexpressed in E. coli BL21 (DE3) pLysS cells. The purified Psychrobacter celer lipase (PCL) was characterized as an alkaline active enzyme and has a molecular mass of around 30 kDa. The PCL was active even at a low temperature and the optimum range was...

A two-month experiment was conducted to evaluate the effect of complex carbon sources on the biofloc system and its influence on Penaeus vannamei culture. Four sources of carbon viz. Tapioca flour (BFTf), Rice bran (BFRb), Wheat flour (BFWf), Rice Flour (BFRf), and biofloc were generated, the absence of CHO being considered as control (C). The expe...

A 60 days nursery-based co-culture of Indian white shrimp, Penaeus indicus along with seaweed, Gracilaria tenuistipitata trial was carried out in Recirculatory Aquaculture System (RAS) of 6000 Liters capacity tank (FRP, reinforced fibre plastics) by using biofloc technology (BFT) to evaluate the growth, survival performance and immune potential. Ac...

The beneficial effects of two probiotic bacterial strains Marinilactibacillus piezotolerans and Novosphingobium sp. during the culture of Indian white shrimp, Penaeus indicus, under biofloc and clear water system were evaluated. The experimental variation were CW1 (M. piezotolerans in clear water), BFT1 (biofloc + M. piezotolerans), CW2 (Novosphing...

Intensified aquaculture is becoming a major stake in the global demand for animal protein. The enhancement in the aquaculture practices and management of resources can reduce the environmental impact on the aquatic system. This helps in the transition of traditional culture practice to the intensified sustainable culture system. On this note, biofl...

Experiments were conducted to explore the colonization of beneficial bacteria in shrimp Penaeus vannamei grown in different sources of biofloc and clear water. Beneficial effect in terms of extracellular enzyme production and antibiofilm activity of the isolated strains was determined. Heterotrophic bacterial population were isolated by using diffe...

Lactobacillus mucosae strain AN1 isolated from sheep milk and characterized for its probiotic suitability. In vitro evaluation of critical gut endurance properties of this strain were assessed by different screening methods such as bile salt, gastric acid, lysozyme tolerance assays, hemolytic, cholesterol reduction properties, and HT-29 cell line a...

In this present study, a gene (ent-B) encoding the bacteriocin enterocin-B was cloned, overexpressed and purified from Enterococcus faecium por1. The molecular weight of the bacteriocin enterocin-B was observed around 7.2 kDa and exhibited antimicrobial activity against several human pathogenic bacteria. The antimicrobial activity of cloned enteroc...

A gene coding lipase from Bacillus sp. PU1 was cloned and expressed in E. coli BL21(DE3) pLysS. The purified lipase has a molecular weight of 23 kDa, is highly alkaline (pH range 8-10) and mesophilic (20–50 °C). Three dimensional structure of the lipase was modeled by comparative homology and identified as a typical serine lipase by the presence of...

An extracellular lipase produced by marine fish intestinal isolate Halobacillus sp. AP-MSU 8 was purified and characterized. The lipase was purified to homogeneity using ammonium sulphate precipitation, DEAE-Sepharose anion exchange chromatography and Sephadex G-75 gel filtration chromatography. The overall purification protocols resulted in 25 % y...

Protease enzyme from estuarine sediment bacterium Bacillus flexus was purified by using two steps such as ammonium sulphate precipitation (37.25% enzyme yield and 2.75 fold purification) and sephadex G-75 gel filtration chromatography (9.38% yield and 10.62 fold purification). Molecular weight of the purified protease detected was 44.3 kDa. The pro...

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A proteolytic Bacillus thuringiensis AP-CMST was isolated from the gut of estuarine fish Etroplus suratensis and the factors affecting protease production in solid-state fermentation was assessed using trash fish-Odonus niger meal and the production was utmost in pH 7 and temperature 40°C. The effect of carbon and nitrogen sources on the protease p...

A bacterium Staphylococcus cohnii AP-CMST, capable of producing lipase was isolated from the gut of marine fish Sardinella longiceps. The lipase production was investigated in solid-state fermentation experiment using anchovy processing wastes. The time course for lipase production inferred that 72 h was the optimum duration for higher lipase produ...

The bacterium Bacillus altitudinis AP-MSU, able to produce esterase was isolated from the gut of marine fish Sardinella longiceps. The esterase production was investigated in solid-state fermentation experiment using various fish processing waste meal. Among the tested fish processing wastes, red grouper waste emerged as the best source for higher...

The possibility of utilising anchovy waste meal as a substrate for protease production by the fish gut isolate Serratia proteamaculans AP-CMST was assessed through solid-state fermentation. A time course for protease production revealed 72 h to be the optimum duration for higher production (146.24 U/g). The most suitable pH, temperature and moistur...

The production of extracelluar phytase by Pseudomonas AP-MSU 2 isolated from the estuarine fish Etroplus suretensis was investigated in solid-state fermentation. Screening of agroindustrial substrates revealed that cassava waste meal produced a higher amount of phytase. The most suitable nitrogen source to enhance phytase production was found to be...

Effect of different wavebands of ultraviolet radiation (UV-A and UV-B) on pigments profile of south Indian macroalgae Gracillaria edulis and Hypnea musciformis was assessed under invitro conditions. In general, the Chlorophyll - a (Chl a) was higher than the Chlorophyll - b (Chl b) content in the candidate seaweeds. The long wavelength UV-A progres...

A bacterium producing extracellular lipase was isolated from the gut of the estuarine shrimp Penaeus indicus and identified as Staphylococcus epidermidis. Lipase production was investigated in shake-flask culture. Cod liver oil was found to be a suitable triglyceride to increase lipase production, at an optimum concentration of 1.5%. Investigating...

Extracellular lipase production by Staphylococcus epidermidis CMST Pi 2 isolated from the intestine of shrimp Penaeus indicus has been investigated in shake-flask experiment using different preparations of tuna-processing waste such as raw fish meat, defatted fish meat, alkali hydrolysate, and acid hydrolysate as nitrogen source. Among the tested t...

Extracellular protease production by Bacillus cereus isolated from the intestine of fish Mugil cephalus has been investigated in shake-flask experiment using different preparations of tuna-processing waste such as raw fish meat, defatted fish meat, alkali hydrolysate, and acid hydrolysate as nitrogen source. Among the tuna preparations tested, defa...

Production of extracellular lipase in submerged culture of Serratia rubidaea has been investigated. The lipase production was optimized in shake flask experiments. The observed pH and temperature range optimum for maximum lipase production were 7–8 and 30–40 °C, respectively. With a selected nitrogen source, casein ((6.5±0.015) U/mL) and soytone ((...

Production of extracellular lipase in submerged culture of Serratia rubidaea has been investigated. The lipase production was optimized in shake flask experiments. The observed pH and temperature range optimum for maximum lipase production were 7-8 and 30-40°C, respectively. With a selected nitrogen source, casein ((6.5±0.015) U/mL) and soytone ((9...