Padungsak Suksa-Ard

Walailak University · School of Agricultural Technology

Catharanthus roseus (L.) G. Don is an important herbal plant. There are two important alkaloids, vinblastine and vincristine, use in anti-cancer drugs. In this study production of the two alkaloids was enhanced in C. roseus cell cultures, in a Murashige and Skoog (MS) liquid medium supplemented with 1.5 mg/L 2,4-D, 0.5 mg/L kinetin and 30 g/L sucro...

The in vitro germplasm conservation of mangosteen (Garcinia mangostana L.) and longkong (Lansium domesticum Corr.) was investigated by suppression of shoot growth using growth retardants and increasing the osmotic potential of culture medium. Abscisic acid (ABA) at 2 mg/l affected to suppress shoot growth of mangosteen and longkong more than paclob...

To identify adequate conditions to conserve papaya shoots in vitro, attempts were made to suppress the growth of shoots by lowering the temperature, applying growth retardants and increasing the osmotic potential of the culture medium. Low temperature below 16°C markedly depressed shoot growth. The shoots conserved at 16°C still survived at a high...

A study was made of the effects of rooting substrates on in vitro rooting of Anthurium andraeanum L. cv. Avanti, orange flower. Initiation of root was attempted in several rooting substrates with modified ½ MS medium supplemented with 30 g/l sucrose. The cut end of the shoot was dipped in 2.5 g/l indole-3-butyric acid (IBA) before insertion in subs...

The influence of the rooting substrate on the root development of papaya in vitro was studied. The rooting of microcuttings started after the swelling of the basal ends. After 4 weeks of culture, the rooting rate was highest in the starch medium at 96%, followed by the agar, rockwool and vermiculite media. In the gellan gum medium, only 8% of the m...