Pablo Tsukayama

Pablo Tsukayama
Universidad Peruana Cayetano Heredia | UPCH · Departamento de Ciencias Celulares y Moleculares

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72
Publications
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883
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Publications

Publications (72)
Preprint
Background Antibiotic resistance is a leading cause of death, with the highest burden in low-resource settings. There is limited evidence on the potential for water, sanitation, and hygiene (WASH) infrastructure to reduce the burden of antibiotic resistance in humans. Methods We used geospatially tagged human gut metagenomes and household survey d...
Article
Peru celebrates 200 years of independence in 2021. Over this period of independent life, and despite the turbulent socio-political scenarios, from internal armed conflict to economic crisis to political instability over the last 40 years, Peru has experienced major changes on its epidemiological and population health profile. Major advancements in...
Article
Full-text available
Poultry farming represents Peru’s primary food animal production industry, where antimicrobial growth promoters are still commonly used, exerting selective pressure on intestinal microbial populations. Consumption and direct animal-to-human transmission have been reported, and farmworkers are at high risk of colonization with resistant bacteria. We...
Article
Full-text available
Background Bartonella bacilliformis is the aetiological agent of Carrión's disease, a biphasic and highly lethal illness formerly restricted to the South American Andes that is now spreading to adjacent areas. Reliable serodiagnostic approaches and vaccines are urgently needed. In this study, we aimed to identify immunodominant proteins of B bacill...
Conference Paper
Full-text available
El surgimiento de nuevas variantes de SARS-CoV-2 con características diferentes de transmisión, virulencia o escape inmunológico es un problema actual para controlar la COVID-19, especialmente en países de ingresos bajos y medianos donde la transmisión sigue siendo alta y la vacunación progresa lentamente. Perú ha sido severamente golpeado por la C...
Article
Full-text available
Here, we report a draft genome sequence of a bla KPC-2 -carrying Citrobacter braakii isolated from pediatric hospital effluent. Genome CF248 represents a multidrug-resistant C. braakii isolate derived from a clinical environment in Peru.
Preprint
Full-text available
We report the emergence of a novel lineage of SARS-CoV-2 in South America, termed C.37. It presents a deletion in the ORF1a gene (Δ3675-3677), also found in variants of concern (VOCs) Alpha, Beta, and Gamma, and seven non-synonymous mutations in the Spike gene (Δ247-253, G75V, T76I, L452Q, F490S, T859N). Initially reported in Lima, Peru, in late De...
Poster
Full-text available
Antimicrobial resistance (AMR) is an ever-growing public health threat worldwide, posing a severe risk to low- and middle-income countries. Poor antibiotic stewardship and inadequate wastewater treatment in healthcare settings may contribute to the environmental dissemination of AMR into municipal wastewaters and other water bodies. Wastewater sam...
Poster
Full-text available
Pigs are natural reservoirs of Campylobacter coli, the second most common cause of human foodborne campylobacteriosis, and pork is the third most consumed animal protein in Peru. Several studies have highlighted the importance of C. coli as a human pathogen due to a higher resistance to different antimicrobials compared to C. jejuni. While studies...
Article
Background Environmental monitoring of enterobacteria in hospital wastewater could be a useful tool to understand the composition of the microbiota in patients, their visits, and healthcare personnel, but it also may be useful for monitoring antimicrobial resistance among healthcare-associated infections in hospitalized patients. The aim of this st...
Article
Full-text available
The widespread and poorly regulated use of antibiotics in animal production in low- and middle-income countries (LMICs) is increasingly associated with the emergence and dissemination of antibiotic resistance genes (ARGs) in retail animal products. Here, we compared Escherichia coli from chickens and humans with varying levels of exposure to chicke...
Article
Full-text available
We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan® system. A standard polymerase chain reaction was used for the detection of the blaTEM, blaCTX-M, blaSHV and blaP...
Article
Full-text available
Providencia stuartii is an opportunistic pathogen of the Enterobacteriales order. Here, we report the 4,594,658-bp draft genome sequence of a New Delhi metallo-␤-lactamase (NDM-1)-producing Providencia stuartii strain that was isolated from an emergency patient in a private clinic in Lima, Peru.
Poster
Full-text available
Campylobacteriosis is the leading cause of foodborne gastroenteritis worldwide, linked to a variety of clinical sequelae and complications such as Guillain-Barré Syndrome (GBS). Campylobacter jejuni infections are commonly associated with the consumption of contaminated chicken meat and presumed to be underreported in Peru, due to difficulty in iso...
Poster
Full-text available
Widespread antibiotic use in human medicine and agriculture has selected for the emergence and spread of various antibiotic resistance (AMR) determinants in microbial populations, posing a major threat to global health. AMR is of particular concern in low- and middle-income countries (LMICs), due to inadequate sanitation, high infectious disease bu...
Poster
In 2018, Tuberculosis (TB) was the leading cause of mortality due to an infectious disease, with an estimated 10 million new cases and 1.5 million deaths. Despite having a low HIV prevalence and a successful TB control program, Peru still has the second-highest estimated TB rates in the Americas and reported 29% of all multidrug-resistant (MDR) cas...
Article
Full-text available
Antimicrobial resistance (AMR) is a growing public health challenge that is expected to disproportionately burden lower- and middle-income countries (LMICs) in the coming decades. Although the contributions of human and veterinary antibiotic misuse to this crisis are well-recognized, environmental transmission (via water, soil or food contaminated...
Article
Full-text available
Bartonella bacilliformis is the biological agent of Carrion’s disease, a vector-borne, life-threatening human bartonellosis restricted to South America. Here, we report the complete genome sequence of B. bacilliformis KC584 (ATCC 35686). Although it is commonly used as a reference strain, to date, its complete genome has not been published.
Poster
Full-text available
Poultry farming is one of the main food animal production industries in Peru. However, the common practice of using antibiotics as growth promoters exerts selective pressure on the microbial populations that inhabit the intestines of chickens. Escherichia coli is the most common intestinal commensal in vertebrates, and the emergence of multidrug-re...
Chapter
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“Water pollution in Peru is as old as the existence of cities, because rivers and seas have served and continue to serve as final disposal points for sewage” (ONERN, 1985).
Article
Full-text available
Antibiotic exposure results in acute and persistent shifts in the composition and function of microbial communities associated with vertebrate hosts. However, little is known about the state of these communities in the era before the widespread introduction of antibiotics into clinical and agricultural practice. We characterized the fecal microbiot...
Article
Antibiotic-resistant infections annually claim hundreds of thousands of lives worldwide. This problem is exacerbated by exchange of resistance genes between pathogens and benign microbes from diverse habitats. Mapping resistance gene dissemination between humans and their environment is a public health priority. Here we characterized the bacterial...
Article
Full-text available
In South America, various species of Leishmania are endemic and cause New World tegumentary leishmaniasis (NWTL). The correct identification of these species is critical for adequate clinical management and surveillance activities. We developed a real-time polymerase chain reaction (PCR) assay and evaluated its diagnostic performance using 64 archi...
Data
Results of conventional diagnostic tests for 14 clinical samples reported as false negatives. Conventional diagnostic tests and real-time PCR were negatives but the kDNA PCR assay was positive. Since melting peaks were not observed, Leishmania species could not be identified for these 14 samples. (DOC)
Data
Results of conventional diagnostic tests for 6 clinical samples reported as false positives. At least one conventional diagnostic test and one real-time PCR assay were positives but the kDNA PCR assay was negative. Two samples yield melting peaks for both MPI and 6PDG real-time PCR assays and the involved Leishmania species were identified. Four sa...
Data
Seventy two clinical samples comparing Leishmania speciation by MLST and by our novel real-time PCR assay. A 100% concordance between MLST and real-time PCR assay was obtained when this subset of samples were compared in parallel. The shaded rows correspond to reference strains (sequenced in [18]) that were used as comparison. SNPs in MPI and 6PGD...
Article
Full-text available
Monitoring changes in the frequencies of drug-resistant and -sensitive genotypes can facilitate in vivo clinical trials to assess the efficacy of drugs before complete failure occurs. Peru changed its national treatment policy for uncomplicated malaria to artesunate (ART)-plus-mefloquine (MQ) combination therapy in the Amazon basin in 2001. We geno...
Article
Full-text available
Bacterial pathogens are frequently distinguished by the presence of acquired genes associated with iron acquisition. The presence of specific siderophore receptor genes, however, does not reliably predict activity of the complex protein assemblies involved in synthesis and transport of these secondary metabolites. Here, we have developed a novel qu...
Data
Primers used in construction of UTI89 mutants. (0.02 MB DOC)
Data
Dendrogram, PFGE patterns, siderophore status and hemolytic properties of patient strains collected for comparison of urinary strains with coexisting rectal strains. (4.89 MB TIF)
Data
CID fragmentations used to identify and quantify siderophores by LC-MS/MS. (0.03 MB DOC)
Article
Full-text available
All New World Leishmania species can cause cutaneous lesions, while only Leishmania (Viannia) braziliensis has been associated with mucosal metastases. Multilocus enzyme electrophoresis (MLEE) is the optimal standard for species identification but is slow and costly. New methods for species identification are needed to ensure proper identification...

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Projects

Projects (3)
Project
Establish a processing, sequencing and analysis system of SARS-CoV-2 genomes to evaluate the transmission and evolution in Peru, and generate epidemiological data in real time that is interpretable and actionable by public health agencies. Recently, we are focusing our efforts to study VOI Lambda (C.37) Developed at: Microbial Genomics Laboratory (FACIEN UPCH) and Instituto de Medicina Tropical "Alexander von Humboldt" of the Universidad Peruana Cayetano Heredia.
Project
Continued analyses of factors affecting the prevalence of resistance, and the spread of underlying genes within and among E. Coli and Klebsiella taxa in Peru, using a powerful combination of microbial epidemiologic and genomic approaches.
Project
Genomic epidemiology and antibiotic resistance genes in Campylobacter from human and animal hosts in Peru.