Pablo López-Gómez

Centro de estudios científicos y avanzados-Irapuato · Biotecnología de Plantas

La inducción de la embriogénesis somática en café (Coffea spp.) es afectada por el tipo de explante y el medio de cultivo. Este trabajo se efectuó para obtener el tipo de explante foliar y el medio de cultivo óptimos para la inducción de la embriogénesis somática en 13 genotipos mejorados de Coffea spp. Se establecieron explantes de hojas inmaduras...

RESUMEN El objetivo de este estudio fue evaluar el efecto de biofertilizantes y medios de cultivo sobre la tolerancia al establecimien-to aséptico y la inducción de la organogénesis en banano. Se utilizaron explantes de hijuelos de dos genotipos de Musa cavendish AAA, cultivares 'Clon Francés' y 'Clon Oaxaqueño'. Se evaluaron tres aplicaciones de b...

The somatic embryogenesis can be affected by explants' oxidation when a common method of disinfection is. In the Experimental Field Rosario Izapa in 2006, the tolerance to disinfectants of leaf explants of coffee genotypes was determined by the median lethal dose (LD50) and based on its tolerance the optimal disinfectant for aseptic establishment w...

Hylocereus undatus (Haw.), también conocida como el cactus que florece de noche, es una planta trepadora que con frecuencia se encuentra sobre rocas o como epífita; es nativa de los bosques tropicales de México y Centro América, y se encuentra ampliamente distribuida como cultivo en el mundo. El fruto de H. undatus es comestible y es conocido p...

Jatropha curcas L., conocida en México como piñoncillo, es una especie productora de aceite, la cual ha recibido gran atención en recientes años al ser utilizada como fuente para la elaboración de biodiesel debido que sus semillas llegan a producir de 46 a 64% de aceite. Sin embargo, la producción de Jatropha ha demostrado limitado éxito debido al...

Genetic improvement of cocoa (Theobroma cacao L.) could be assisted by low-cost and highly informative molecular techniques. In this study, primers for the detection of sequence-related amplified polymorphism (SRAP) and intron targeted amplified polymorphism (ITAP) were used for the molecular characterization of the genus Theobroma. Genotypes Carme...

Rumen development depends on the intake of solid food that is fermented into volatile fatty acids that stimulate the development of the rumen papillae in calves. The starter feeding can promote the growth of papillae in the rumen and as a consequence an earlier weaning. We evaluated the effects of calf starter on ruminal development, and productive...

Objetivo: Evaluar el efecto del consumo de iniciador en la respuesta productiva pos destete de becerros del sistema de doble propósito. Diseño/metodología/aproximación: 10 becerros con peso vivo promedio al nacimiento de 34.5 ± 4.8 kg fueron distribuidos en dos tratamientos: BL = becerros alimentados con leche + pasto Taiwán (Pennisetum purpureum)...

Ninety-nine accessions of the Cocoa Germplasm Bank in the Rosario Izapa Experimental Station, INIFAP, Mexico, were studied in order to evaluate the genetic diversity and thus lay the foundation for a long-term conservation program of materials representative of diversity through a core collection. Fourteen microsatellite (SSR’s) markers were used....

RESUMEN En este estudio se investigó la variación somaclonal de plantas regeneradas in vitro por organogénesis directa (OD) a partir de explantes de yemas florales en proliferación, de Musa sp., al utilizar marcadores tipo secuencias internas de regiones simples repetidas (ISSR), polimorfismo amplificado de secuencias relacionadas (SRAP) y polimorf...

The combination of salts, hormones and the gaseous environment in vitro could affect the expression of somatic embryogenesis. The effect of the combination of two culture media and inhibitors of ethylene induction of embryogenic callus of Coffea canephora P., and their ability to proliferate and form embryos was studied. five leaf explants of C. ca...

The combination of salts, hormones and the gaseous environment in vitro could affect the expression of somatic embryogenesis. The effect of the combination of two culture media and inhibitors of ethylene induction of embryogenic callus of Coffea canephora P., and their ability to proliferate and form embryos was studied. five leaf explants of C. ca...

We investigated somaclonal variation in regenerants of bananas derived from in vitro indirect somatic embryogenesis and direct organogenesis from floral buds of only one mother plant, using inter simple sequence repeat (ISSR), sequence-related amplified polymorphims (SRAP) and intron targeted amplified polymorphism (ITAP) analysis. Inmature male fl...

The rates of maturation and germination even represent a bottleneck to cultivars of Musa acuminata (AAA, Cavendish). The aim of this work was to evaluate culture media to optimize the phases of maturation and germination of somatic embryos of cv. Enano Gigante of Musa. At the maturation stage, two media culture was evaluated (MS and MS + 1 µM AIA +...

Specific culture media are used for lipolytic fungi and bacteria growth. It is possible that more complex culture media, used for plant tissue culture, allows a higher expression of fungi and bacteria growth. The aim was to evaluate the effect of the addition of vegetable oil in different culture media for micropropagation, on growth of fungi and b...

In Musa (Musaceae family) as for other angiosperms, somatic embryo formation from somatic cells exemplify a distinctive phenomenon of plant cell developmental plasticity. Somatic embryogenesis (SE) through embryogenic cell suspension (ECS) cultures is an important milestone method for accelerating bananas mass-propagation due to its high regenerati...

The agave papalote (Agave cupreata Trelease) which is endemic to the Guerrero state, Mexico, is propagated exclusively by seed, but it is overutilized and knowledge of its diversity is needed for its protection. Therefore, the study aimed to determine the morphological variation in wild and cultivated populations of Agave cupreata in five areas of...

RESUMEN El piñón azul o maxi piñón Pinus maximar-tinezii Rzedowski es una especie endémica en peligro de extinción debido a la alteración de sus poblaciones en su hábitat natural y que ha sobrevivido a una restricción ge-nética extrema, además es considerado el más raro de los pinos piñoneros. En este trabajo se evaluó la respuesta rizogénica de br...

Pinus maximartinezii Rzedowski is an endemic specie in danger of extinction due to population alteration in the wild and has survived extreme genetic restriction, it is also considered the rarest of pines. In this study rhizogenic response of Pinus maximartinezii sprouts, obtained from zygotic embryos regenerated via organogenesis subjected to in v...

Somatic embryogenesis induction in coffee (Coffea spp.) is affected by explant type and culture medium. This work was done in order to obtain the optimal type of foliar explant and culture medium for somatic embryogenesis induction in 13 selected genotypes of Coffea spp. Explants of immature (Hi), young (Hy) and mature leaves (Hut) were compared in...