Pablo A. Kler

Pablo A. Kler
National Scientific and Technical Research Council | conicet · Centro de Investigación de Métodos Computacionales

Dr. Bioeng.

About

41
Publications
11,991
Reads
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900
Citations
Introduction
Pablo A. Kler currently works at the Centro de Investigación de Métodos Computacionales, National Scientific and Technical Research Council. Pablo does research in Computational Microfluidics and microscale transport phenomena.
Additional affiliations
September 2014 - present
Universidad Tecnológica Nacional
Position
  • Professor (Assistant)
March 2014 - present
National Scientific and Technical Research Council (CONICET)
Position
  • Research Assistant
October 2013 - March 2014
University of Tuebingen
Position
  • PostDoc Position
Education
July 2015 - July 2020
Universidad Nacional del Litoral
Field of study
  • Facultad de Humanidades y Ciencias
April 2006 - March 2011
Universidad Nacional del Litoral
Field of study
  • Computational Mechanics
March 1999 - March 2006
National University of Entre Rios
Field of study
  • Biomedical Engineering

Publications

Publications (41)
Article
Full-text available
The integration of smartphones and microfluidics is nowadays the best possible route to achieve effective point-of-need testing (PONT), a concept increasingly demanded in the fields of human health, agriculture, food safety, and environmental monitoring. Nevertheless, efforts are still required to integrally seize all the advantages of smartphones,...
Article
Full-text available
Paper-based microfluidics has grown continuously over the last few years. One of the most important characteristics of paper-based microfluidic devices is the ability to pump fluids with the single action of capillary forces. However, fluid flow control in paper-based microfluidic devices has been studied primarily through empirical approaches; and...
Article
Full-text available
Paper-based electromigrative separations have recently gained relevance due to the rise of paper-based microfluidic devices and their combination with electrophoretic methods, used for many different analytical applications [1]. A new version of the open source toolbox electroMicroTransport for the numerical solution of electromigrative separations...
Article
Full-text available
A new tool for the solution of electromigrative separations in paper‐based microfluidics devices is presented. The implementation is based on a recently published complete mathematical model for describing these kind of separations and was developed on top of the open source toolbox electroMicroTransport, based on OpenFOAM®, inheriting all its feat...
Article
Full-text available
A novel method for electroosmotic flow (EOF) measurement on paper substrates is presented; it is based on dynamic mass measurements by simply using an analytical balance. This technique provides a more reliable alternative to other EOF measurement methods on porous media. The proposed method is used to increase the amount and quality of the availab...
Article
Full-text available
A complete mathematical model for electromigration in paper‐based analytical devices is derived, based on differential equations describing the motion of fluids by pressure sources and EOF, the transport of charged chemical species and the electric potential distribution. The porous medium created by the cellulose fibers is considered like a networ...
Article
Full-text available
Microfluidic paper‐based analytical devices (μPADs) allow user‐friendly and portable chemical determinations, although they provide limited applicability due to insufficient sensitivity. Several approaches have been proposed to address poor sensitivity in μPADs, but they frequently require bulky equipment for power and/or read‐outs. Universal seria...
Article
We discuss several possible phenomena in electrophoretic systems with complexing agents present in the background electrolyte. In our previous work, we extended the linear theory of electromigration with the first-order nonlinear term, which originally applied to acid-base equilibria only, by generalizing it to any fast chemical equilibria. This ex...
Article
This work describes a method to fabricate three-dimensional paper microfluidic devices in one step, without the need of stacking layers of paper, glue, or tape. We used a non-transparent negative photoresist that allows patterning selectively (vertically) the paper, creating systems of two or three layers, including channels. To demonstrate the cap...
Article
The linear theory of electromigration, including the first‐order nonlinear approximation, is generalized to systems with any equilibria fast enough to be considered instantaneous in comparison with the time‐scale of peak movement. For example, this theory is practically applied in the electrokinetic chromatography (EKC) mode of the capillary zone e...
Article
Full-text available
We present a novel simulation toolbox for electromigrative problems. The formulation presented includes all the fields, interaction and effects that configure both electrokinetic and electroosmotic phenomena for studying capillary and chip electromigrative separation methods. This simulation toolbox was developed by using the Finite Volume Method (...
Conference Paper
Full-text available
The increment of food market requirements and competitiveness makes that industry needs more accurate sensing and monitoring tools. Due to the large temporal dynamics necessary to monitor some animal behaviours, the autonomy and storage capacity of the devices involved becomes critical. In this work, we introduce a device specifically developed for...
Article
A simple method for obtaining intrinsic kinetic parameters of photocatalytic, first-order reactions is presented. The method makes use of a microfluidic reactor, which was specifically designed to operate under conditions of negligible mass transfer limitations and involves a reversible sealing that allows one to test different catalyst films on th...
Article
Full-text available
The generation of concentration gradients is an essential operation for several analytical processes implemented on microfluidic paper-based analytical devices. The dynamic gradient formation is based on the transverse dispersion of chemical species across co-flowing streams. In paper channels, this transverse flux of molecules is dominated by mech...
Article
Full-text available
Lateral flow biosensors (LFB) have become a hot topic in the scientific literature in association with the rapid growing of paper-based microfluidics. Improving the existing LFB technology is a challenging task that demands large experimental efforts. Thus computer simulations are practical tools to assist the development of novel devices, since ru...
Article
Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate o...
Article
Full-text available
Here we present the study on the sample transfer characteristics of two different microfluidic interfaces for two-dimensional capillary electrophoresis. These interfaces were manufactured using two different microfabrication technologies: one was obtained via the classical photolithography - wet etching - anodic bonding process; and the other was o...
Article
One of the greatest challenges in multi-channel networks for electromigrative separation techniques is the control of the leakage of sample constituents and band broadening at the channel intersections in microfluidic devices or capillary-chip interfaces, which can be achieved using fixed bias or pullback potentials. These may be implemented as the...
Article
Full-text available
A simple mathematical model that quantitatively describes the dynamics of analyte capture in lateral flow assays is presented. The formulation accounts for the capillary-driven flow through the porous membrane, the advective transport of analyte, and the immunoreactions that take place in the detection line. Model predictions match the numerical re...
Article
Full-text available
Isotachophoresis (ITP) has long been used alone but also as a preconcentration technique for capillary electrophoresis (CE). Unfortunately, up to now, its application is restricted to relatively strong acids and bases as either the degree of (de)protonation is too low or the water dissociation is too high, evoking zone electrophoresis. With the com...
Article
Full-text available
Multidimensional electrophoretic separations represent one of the most common strategies for dealing with the analysis of complex samples. In recent years we have been witnessing the explosive growth of separation techniques for the analysis of complex samples in applications ranging from life sciences to industry. In this sense, electrophoretic se...
Article
High production output of solid pharmaceutical formulations requires fast methods to ensure their quality. Likewise, fast analytical procedures are required in forensic sciences, for example at customs, to substantiate an initial suspicion. We here present the design and the optimization of an instrumental setup for rapid and non-invasive character...
Article
This work presents the design and characterization of an optimized attenuated total reflection (ATR) microfluidic cell to assess intrinsic kinetic parameters of reactions at the liquid/solid interface under chemical control. A theoretical and computational investigation of convection, diffusion, and adsorption is presented. Transport dynamics in tr...
Article
Full-text available
This article discusses high performance numerical simulations of electrokinetic flow and transport phenomena in microfluidic chips. Modeling grounds on conservation equations of mass, momentum and electric charge in the framework of continuum mechanics. Two examples of interest in microfluidics are considered as study cases. Three dimension effects...
Article
Full-text available
Two-dimensional electrophoretic separations are one of the most promising tools for the continuously growing needs of different bioanalytical fields such as proteomics and metabolomics. In this work we present the design and the implementation of a two-dimensional electrophoretic separation coupled to mass spectrometry. We started our work studying...
Article
Full-text available
Electrophoresis is the motion of charged particles relative to the surrounding liquid under the influence of an external electric field. This electrochemical transport process is used in many scientific and technological areas to separate chemical species. Modeling and simulation of electrophoretic transport enables a better understanding of the ph...
Article
Full-text available
In this paper we introduce and describe an efficient thread-safe matrix library for computing element/cell residuals and Jacobians in Finite Elements and Finite Volume-like codes. The library provides a wide range of multi-index tensor operations that are normally used in scientific numerical computations. The library implements an algorithm for ch...
Article
The development of a novel microfluidic device that includes a circular microchannel and integrated electrodes for DNA electrophoresis is reported. The geometry of the separation channel and the arrangement of the embedded electrodes provide several advantages in relation to conventional linear microchannels. The paper describe the design, modeling...
Article
The work presents an alternative method for the manufacture of micropores by using the combination of laser ablation and wet etching. The process of laser ablation was done on silicon (Si) wafers with silicon nitride (Si3N4) used as a sacrificial layer. The size of the pores was carefully controlled by following an optical technique. The method exh...
Article
Full-text available
This work presents two software components aimed to relieve the costs of accessing high-performance parallel computing resources within a Python programming environment: MPI for Python and PETSc for Python.MPI for Python is a general-purpose Python package that provides bindings for the Message Passing Interface (MPI) standard using any back-end MP...
Article
Full-text available
Electrophoretic separations comprise a group of analytical techniques such as capillary zone electrophoresis, isoelectric focusing, isotachophoresis, and free flow electrophoresis. These techniques have been miniaturized in the last years and now represent one of the most important applications of the lab-on-a-chip technology. A 3D and time-depende...
Article
Full-text available
This work reports our attempts to facilitate the access to high-performance parallel computing resources within a Python programming environment. The outcome of this effort are two open source and public domain packages, MPI for Python (known in short as mpi4py) and PETSc for Python (known in short as petsc4py). MPI for Python [1, 2, 3], is an open...
Article
This work presents the numerical simulation of multidimensional electrophoretic separations in microfluidic devices. Calculations are performed by using the finite element method (FEM). A numerical prototype for free flow isoelectric focusing and capillary zone electrophoresis is studied. The separation of ten amphoteric species is successfully per...
Article
Full-text available
This article discusses high performance numerical simulations of electrokinetic flow and transport phenomena in microfluidic chips. Modeling grounds on conservation equations of mass, momentum and electric charge in the framework of continuum mechanics. Two examples of interest in microfluidics are considered as study cases. Three dimension effects...
Article
Full-text available
Electrophoretic methods are separation techniques based on the mobility of ions under the action of an external electric field. These techniques, which are widely used in chemical and biochemical analysis, have been miniaturized in the last years and now represent one of the most important applications of the lab-on-a-chip technology. In this work,...
Article
Full-text available
Microfluidic devices like those used in chemical and biomedical applications basically consist of different networks of microchannels that interconnect chambers and reservoirs. The transport of fluids throughout the network is driven by pressure gradients, electric fields, or a combination of the two, which yields to the so-called electrokinetic fl...

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