Pablo Cavagnaro

National Scientific and Technical Research Council | conicet · INTA EEA La Consulta and Universidad Nacional de Cuyo

Fresh-cut produce have become widely popular, increasing vegetable consumption in many parts of the word. However, they are more perishable than unprocessed fresh vegetables, requiring cold storage to preserve their quality and palatability. In addition to cold storage, UV radiation has been used experimentally to try to increase nutritional qualit...

Background Plant breeding allows altering the genetic structure of plants to meet human needs. The use of radiation technology for inducing mutations and -thereby- new phenotypic variants has become increasingly common as a tool for developing new crops. The aim of this study was to determine the effective gamma irradiation dose for inducing mutati...

To estimate genetic and environmental effects on traits associated with garlic functional value and postharvest quality, the content of total organosulfur compounds, phenolics, and solids were determined in selected garlic cultivars grown at four locations in Mendoza, Argentina. To this end, an initial experiment considered five cultivars grown in...

Carrots require a certain number of cold hours to become vernalized and proceed to the reproductive stage, and this phenomenon is genotype-dependent. Annual carrots require less cold than biennials to flower; however, quantitative variation within annuals and biennials also exists, defining a gradient for vernalization requirement (VR). The floweri...

As a means to evaluate the potential of carrot anthocyanins as food colorants and nutraceutical agents, we investigated the physicochemical stability and antioxidant capacity of purple carrot extracts under different pH (2.5–7.0) and temperature (4–40 ◦C) conditions, in comparison to a commercial synthetic (E131) and a natural grape-based (GRP)...

Climate is determinant for grapevine geographical distribution, berry attributes, and wine quality. Due to climate change, a 2–4 °C increase in mean diurnal temperature is predicted by the end of the century for the most important Argentine viticulture region. We hypothesize that such temperature increase will affect color intensity and other quali...

The native forage, false Rhodes grass [Leptochloa crinita (Lag.) P.M. Peterson and N.W. Snow], represents an important component of the arid and semi-arid rangelands of the Americas, due to its large area of distribution and its tolerance to drought and cattle grazing. Precise information on its nutritive value and the factors that influence this t...

In arid regions, revegetation with locally adapted native species can improve forage production and help ameliorate soil degradation. We investigated the effects of 3 sowing dates and 3 sowing rates of Trichloris crinita cv. Chamical-INTA, a perennial forage grass native to arid and semi-arid regions, on pasture establishment parameters in the Arge...

In purple carrots, anthocyanin pigmentation can be expressed in the entire root, or it can display tissue specific-patterns. Within the phloem, purple pigmentation can be found in the outer phloem (OP) (also called the cortex) and inner phloem (IP), or it can be confined exclusively to the OP. In this work, the genetic control underlying tissue-spe...

Plant breeding allows altering the genetic structure of plants to meet human needs. The use of radiation technology for inducing mutations and –thereby- new phenotypic variants has become increasingly common as a tool for developing new crops. The aim of this study was to determine the effective gamma irradiation dose for inducing mutations in purp...

El híbrido ACONCAGUA INTA proviene de un cruzamiento simple entre Cucurbita maxima x C. moschata. Su interés para la industria del deshidratado se debe a un rendimiento de 50 t·ha- 1 y un 17% en sólidos totales. Dada la presencia de autopolinizaciones en la producción de semilla, es necesario determinar el porcentaje de cruzamientos efectivos. Para...

On the bases of their vernalization requirement (VR) for flowering, a genetically‐conditioned trait, carrots are typically classified as annuals and biennials. A previous study using F2 populations derived from crosses among carrots from different geographical origins revealed a model of two genes and three alleles controlling VR, with dominance of...

Purple or black carrots (Daucus carota ssp. sativus var. atrorubens Alef) are characterized by their dark purple- to black-colored roots, owing their appearance to high anthocyanin concentrations. In recent years, there has been increasing interest in the use of black carrot anthocyanins as natural food dyes. Black carrot roots contain large quanti...

Anthocyanins are natural health promoting pigments that can be produced in large quantities in some purple carrot cultivars. Decoration patterns of anthocyanins, such as acylation, can greatly influence their stability and biological properties and use in the food industry as nutraceuticals and natural colorants. Despite recent advances made toward...

Una forma rápida de caracterizar las propiedades nutracéuticas y sensoriales de las cultivares de ajo es a través del análisis de sólidos totales (que representan la aptitud de conservación), de piruvatos (que representa el picor) y fenoles totales (que representan la capacidad antioxidante o protección cardiovascular). Esta metodología permitiría...

Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-div...

This study characterized genetic diversity in the Argentine garlic germplasm bank by analysis of 10 SSR loci in 73 garlic accessions. A total of 43 alleles and polymorphic information content (PIC) value of 0.38, along with various accessions that could not be differentiated with these SSR markers, suggest a relatively high level of genetic redunda...

Key message Inheritance, QTL mapping, phylogenetic, and transcriptome (RNA-Seq) analyses provide insight into the genetic control underlying carrot root and leaf tissue-specific anthocyanin pigmentation and identify candidate genes for root phloem pigmentation. Abstract Purple carrots can accumulate large quantities of anthocyanins in their root t...

Trichloris crinita (Lag.) Parodi is a perennial forage grass native to—and of wide distribution in—arid and semiarid regions of the American continent. In addition to its good forage quality, this species is articularly valued in these drylands due to its high tolerance to drought, salinity, trampling, and grazing. Genetic and cytogenetic informat...

Purple carrots (Daucus carota ssp. sativus var. atrorubens Alef.) accumulate anthocyanins in their roots, petioles, and other plant parts. These flavonoid pigments represent an excellent dietary source of antioxidant and anti-inflammatory agents. In addition, carrot anthocyanins are also used as food dyes. Compositional variation in carrot root, ma...

Carrot (Daucus carota L.) is an important root vegetable crop that is consumed worldwide and is appreciated for its taste and nutritional content (e.g., provitamin A carotenoids, anthocyanins, vitamins, and other minerals). Carrot genetic research has improved vastly over the past few decades due to advancements in molecular genomic resources devel...

Carrot root carbohydrates, composed mainly of reducing and non-reducing free sugars, influence flavor, total dissolved solids and dry mater content, all quality traits for fresh-market and processing carrots. In the last decades, important advances have been made in biochemistry, physiology and genetics of carrot sugar metabolism. Several enzymes i...

Capsicum is one of the most important and diverse plant taxa, widely used as spice and vegetable worldwide, including Turkey. Germplasm characterization is an essential step for crop breeding. In the present study, we characterized genetic diversity and population structure in a collection of 94 pepper accessions using iPBS (inter-primer binding si...

Carrots are typically classified as annual or biennial, depending on their vernalization requirement for flowering, a trait that is genetically-conditioned. Wild carrots are predominantly annual, requiring less hours of cold temperatures for flower induction, whereas most cultivated carrots are biennial (i.e., they have higher cold temperature requ...

Purple carrots can accumulate large quantities of anthocyanins in their roots and –in some genetic backgrounds- petioles, and therefore they represent an excellent dietary source of antioxidant phytonutrients. In a previous study, using linkage analysis in a carrot F2 mapping population segregating for root and petiole anthocyanin pigmentation, we...

Allium vegetables, such as garlic and onion, have understudied genomes and limited molecular resources, hindering advances in genetic research and breeding of these species. In this study, we characterized and compared the simple sequence repeats (SSR) landscape in the transcriptomes of garlic and related Allium (A. cepa, A. fistulosum, and A. tube...

Trichloris crinita is among the most important native forage grasses in arid regions of America. Despite its importance, molecular resources and sequence data are extremely scarce in this species. In the present study, SSR markers were developed using available DNA sequences from grass taxa phylogenetically-related to Trichloris (Eleusine coracana...

Trichloris crinita is among the most important native forage grasses in arid regions of America. Despite its importance, molecular resources and sequence data are extremely scarce in this species. In the present study, SSR markers were developed using available DNA sequences from grass taxa phylogenetically-related to Trichloris (Eleusine coracana,...

Allium sp. vegetables are widely consumed for their characteristic flavour. Additionally, their consumption may provide protection against cardiovascular disease due to their antiplatelet and antioxidant activities. Although antiplatelet and antioxidant activities in Allium sp. are generally recognised, comparative studies of antiplatelet and antio...

In recent years there has been an effort towards the development of genomic resources in carrot. The number of available sequences for carrot in public databases has increased recently. This has allowed the design of SSRs markers, COS markers and a high-throughput SNP assays for genotyping. Additional molecular tools include the first high-throughp...

We present data on absorption spectra (400–540nm) and concentration of phenolic compounds quercetin, myricetin, kaempferol, rutin, catechin, epicatechin gallate (ECG) and epigallocatechin gallate (EGCG), in yellow, red and white onions. These data are related to the article entitled “Variability in spectrophotometric pyruvate analyses for predictin...

Malbec and Bonarda are the two most widely cultivated grape varieties in Argentina, and their derived red wines are recognized worldwide, being their intense color a major quality trait. The temperature during fruit ripening conditions berries color intensity. In the main viticulture region of Malbec and Bonarda a 2–3 °C increase in temperature has...

Malbec and Bonarda are the two most widely cultivated grape varieties in Argentina, and their derived red wines are recognized worldwide, being their intense color a major quality trait. The temperature during fruit ripening conditions berries color intensity. In the main viticulture region of Malbec and Bonarda a 2-3 ºC increase in temperature has...

Trichloris crinita is a perennial forage grass species native to arid regions of the American continent. Due to its extensive area of distribution, good forage quality, and resistance to drought and grazing, this species is widely utilized as forage and for revegetation purposes in environments with low water availability. Despite its importance, g...

Onion pyruvate concentration is used as a predictor of flavor intensity and nutraceutical value. The protocol of Schwimmer and Weston (SW) (1961) is the most widespread methodology for estimating onion pyruvate. Anthon and Barret (AB) (2003) proposed modifications to this procedure. Here, we compared these spectrophotometry-based procedures for pyr...

Plant organosulfur compounds represent one of the main groups of phytochemicals that evidence an ample spectrum of biological activities. There are two major sources of sulfur-containing compounds in plant foods; Allium vegetables, such as garlic, onion, and leek; and cruciferous vegetables, such as broccoli, cabbage, and cauliflower. Among them, g...

We report a high-quality chromosome-scale assembly and analysis of the carrot (Daucus carota) genome, the first sequenced genome to include a comparative evolutionary analysis among members of the euasterid II clade. We characterized two new polyploidization events, both occurring after the divergence of carrot from members of the Asterales order,...

Consumption of onion has been associated with reduced incidence of chronic diseases. Phenolic, organosulfur and carbohydrate compounds present are largely responsible for these effects. This study examined compositional variation for health-enhancing compounds in a genetically diverse collection of onion cultivars. Total antioxidant activity and ar...

Background: Purple carrots accumulate large quantities of anthocyanins in their roots and leaves. These flavonoid pigments possess antioxidant activity and are implicated in providing health benefits. Informative, saturated linkage maps associated with well characterized populations segregating for anthocyanin pigmentation have not been developed....

Root-knot nematodes limit carrot production around the world by inducing taproot forking and galling deformities that render carrots unmarketable. In warmer climates, Meloidogyne javanica and Meloidogyne incognita are most prevalent. In F2 and F3 progeny from the cross between an Asian carrot resistant to M. javanica, PI 652188, and a susceptible c...

Carrot is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting carrot breeding remain limited. We developed a Diversity Arrays Technology (DArT) platform for wild and cultivated carrot and used it to investigate genetic diversity and to develop a saturated genetic linkage map of carrot. We analyz...

Unlabelled: • Premise of the study: Analyses of genetic structure and phylogenetic relationships illuminate the origin and domestication of modern crops. Despite being an important worldwide vegetable, the genetic structure and domestication of carrot (Daucus carota) is poorly understood. We provide the first such study using a large data set of...

Anthocyanin gene expression has been extensively studied in leaves, fruits and flowers of numerous plants. Little, however, is known about anthocyanin accumulation in roots of carrots or other species. We quantified expression of six anthocyanin biosynthetic genes [phenylalanine ammonia-lyase (PAL3), chalcone synthase (CHS1), flavanone 3-hydroxylas...

The rapid advancement in high-throughput SNP genotyping technologies along with next generation sequencing (NGS) platforms has decreased the cost, improved the quality of large-scale genome surveys, and allowed specialty crops with limited genomic resources such as carrot (Daucus carota) to access these technologies. Here, we designed 4,000 Single...

##Assembly-Data-START## Assembly Method :: Sequencher v. 5.0 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

Allium vegetables serve as sources of antiplatelet agents that may contribute to the prevention of cardiovascular disease. However, onion and garlic, the major Allium species, are usually cooked before consumption. Here, we examined the effect of cooking on onion in vitro antiplatelet activity (IVAA). Two different cooking systems (convection oven...

Polymorphism of simple sequence repeat (SSR) loci was assessed in a collection of 88 carrot (Daucus carota L. subsp. sativus Hoffm.) accessions. The collection comprised cultivars and landraces mainly from Asia, Europe, and North America. Plants were grown in the glasshouse and characterized for root color and shape. Thirty SSR loci were fully char...

Table S1 – Individual genotype transcriptome assemblies. Summary of the number of contigs and singletons obtained for the B493, B493×QAL, B6274 and B7262 individual transcriptome assemblies using different assembly methods. Table S2 – Combined transcriptome assemblies. Summary of the B493, B493×QAL, B6274, and B7262 combined transcriptome assemblie...

Table S3 - EST contigs related to transposable elements. EST contigs related to transposable elements, as indicated by BLAST2GO annotation and their classification into subfamilies based on similarity to RepBase entries. Original annotation was maintained for contigs showing no significant similarity to RepBase. Table S5 - EST containing fragments...

Assembly methods and parameters.

Among next generation sequence technologies, platforms such as Illumina and SOLiD produce short reads but with higher coverage and lower cost per sequenced nucleotide than 454 or Sanger. A challenge now is to develop efficient strategies to use short-read length platforms for de novo assembly and marker development. The scope of this study was to d...

Table S1 - Characteristics of 300 carrot simple sequence repeat markers. SSR type, motif, size, sequence, and primers of 300 GSSRs and BSSRs. Table S2 - Performance of GSSRs and BSSRs in subsets of 7 carrot F2 mapping populations. SSR alleles, polymorphism index, and PCR success2 for seven carrot populations. Table S3 - Accessions used for evaluati...

Program used for finding ORFs in sequences.

The Apiaceae family includes several vegetable and spice crop species among which carrot is the most economically important member, with ~21 million tons produced yearly worldwide. Despite its importance, molecular resources in this species are relatively underdeveloped. The availability of informative, polymorphic, and robust PCR-based markers, su...

The cytogenetic characterization of the carrot genome (Daucus carota L., 2n = 18) has been limited so far, partly because of its somatic chromosome morphology and scant of chromosome markers. Here, we integrate the carrot linkage groups with pachytene chromosomes by fluorescent in situ hybridization (FISH) mapping genetically anchored bacterial art...

Supplement Table S5a: list of primers for 83,472 microsatellites found in Cucumis sativus Gy14 genome sequences (# 000001 to 063346)

This MS Excel file includes supplemental Tables S1 to S4. Supplement Table S1. Characteristics of genomic and EST sequence databases used. Supplement Table S2a. Distribution of di-, tri-, and tetranucleotide repeats in genomic and EST sequences of cucumber. Supplement Table S2b. Distribution of the most frequent penta-, hexa-, hepta- and octanucleo...

Supplement Table S5b: list of primers for 83,472 microsatellites found in Cucumis sativus Gy14 genome sequences (#063347 to 083472).

Cucumber, Cucumis sativus L. is an important vegetable crop worldwide. Until very recently, cucumber genetic and genomic resources, especially molecular markers, have been very limited, impeding progress of cucumber breeding efforts. Microsatellites are short tandemly repeated DNA sequences, which are frequently favored as genetic markers due to th...

We report on the development of a novel splinkerette-based method for generating long end sequences from large insert library clones, using a carrot (Daucus carota L.) BAC library as a model. The procedure involves digestion of the BAC DNA with a 6-bp restriction enzyme, followed by ligation of splinkerette adaptors that serve as primer-annealing s...

Carrot is the most economically important member of the Apiaceae family and a major source of provitamin A carotenoids in the human diet. However, carrot molecular resources are relatively underdeveloped, hampering a number of genetic studies. Here, we report on the synthesis and characterization of a bacterial artificial chromosome (BAC) library o...