Pablo Cárdenas R.- Doctor of Philosophy
- Postdoctoral Research Fellow at Ragon Institute of MGH, MIT and Harvard
Pablo Cárdenas R.
- Doctor of Philosophy
- Postdoctoral Research Fellow at Ragon Institute of MGH, MIT and Harvard
I develop experimental and computational tools to study infectious diseases, from the molecular to the epidemiological.
About
13
Publications
4,653
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179
Citations
Introduction
Interested in infectious disease, synthetic and systems biology, mathematical and computational modeling, and the ethics involved in these areas –as well as all of science– in the context of improving public health and social welfare. Enthusiastic supporter of open science, open source and open culture.
Current institution
Additional affiliations
August 2018 - August 2024
Position
- Graduate Research Assistant
Description
- Created molecular and computational tools to control the malaria parasite’s ability to cause disease and enable its use as a platform for systems and synthetic biology as a PhD student at the Niles Lab in MIT BE.
January 2018 - July 2018
Position
- Researcher
Description
- Researching the emergence of bacterial persistence as well as noise and interference in synthetic biology circuits using microfluidics and high-throughput microscopy at the Paulsson Lab, Dept. Systems Biology, HMS
August 2017 - January 2018
Eligo Bioscience
Position
- Research Intern
Description
- Research intern in synthetic biology, phage and bacterial strain engineering.
Education
August 2018 - August 2024
July 2013 - June 2018
Publications
Publications (13)
CRISPRi-mediated gene regulation allows simultaneous control of many genes. However, highly specific sgRNA-promoter binding is, alone, insufficient to achieve independent transcriptional regulation of multiple targets. Indeed, due to competition for dCas9, the repression ability of one sgRNA changes significantly when another sgRNA becomes expresse...
Functional characterization of the multitude of poorly described proteins in the human malarial pathogen, Plasmodium falciparum, requires tools to enable genome-scale perturbation studies. Here, we present GeneTargeter (genetargeter.mit.edu), a software tool for automating the design of homology-directed repair donor vectors to achieve gene knockou...
Antibiotic-induced alterations in the gut microbiota are implicated in many metabolic and inflammatory diseases, increase the risk of secondary infections and contribute to the emergence of antimicrobial resistance. Here we report the design and in vivo performance of an engineered strain of Lactococcus lactis that altruistically degrades the widel...
Genomics is fundamentally changing epidemiological research. However, systematically exploring hypotheses in pathogen evolution requires new modeling tools. Models intertwining pathogen epidemiology and genomic evolution can help understand processes such as the emergence of novel pathogen genotypes with higher transmissibility or resistance to tre...
The human malaria parasite Plasmodium falciparum is globally widespread, but its prevalence varies significantly between and even within countries. Most population genetic studies in P. falciparum focus on regions of high transmission where parasite populations are large and genetically diverse, such as sub-Saharan Africa. Understanding population...
Genomics is fundamentally changing epidemiological research. However, systematically exploring hypotheses in pathogen evolution requires new modeling tools. Models intertwining pathogen epidemiology and genomic evolution can help understand processes such as the emergence of novel pathogen genotypes with higher transmissibility or resistance to tre...
Functional characterization of the multitude of poorly-described proteins in the human malarial pathogen, Plasmodium falciparum , requires tools to enable genome-scale perturbation studies. Here we present GeneTargeter (genetargeter.mit.edu), a software tool for automating the design of homology-directed repair donor vectors to achieve gene knockou...
CRISPRi-mediated gene repression allows simultaneous control of many genes. However, despite highly specific sgRNA-promoter binding, multiple sgRNAs still interfere with one another by competing for dCas9. We created a dCas9 regulator that adjusts dCas9 concentration based on sgRNAs' demand, mitigating competition in CRISPRi-based logic gates. The...
Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and ϕSan23 phage. Sampl...
Este trabajo analiza las relaciones entre ciencia, tecnología y comunidad. Se argumenta que las dinámicas entre las tres, en el marco de los cambios en la ciencia a lo largo del siglo XX, producen la brecha entre el descubrimiento científico y el impacto positivo en la comunidad. Esta brecha en traslación científica puede cerrarse democratizando a...
