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Introduction
Current institution
Åkerblå AS
Current position
- Veterinarian
Publications
Publications (32)
The cellular prion protein (PrPC
) is a medium-sized glycoprotein, attached to the cell surface by a glycosylphosphatidylinositol
anchor. PrPC is encoded by a single-copy gene, PRNP, which is abundantly expressed in the
central nervous system and at lower levels in non-neuronal cells, including those of the immune system.
Evidence from experimental...
Background
The cellular prion protein (PrPC) is an evolutionary conserved protein abundantly expressed not only in the central nervous system but also peripherally including the immune system. A line of Norwegian dairy goats naturally devoid of PrPC (PRNPTer/Ter) provides a novel model for studying PrPC physiology. Methods
In order to explore putat...
The cellular prion protein (PrPC) has been extensively studied because of its pivotal role in prion diseases; however, its functions remain incompletely understood. A unique line of goats has been identified that carries a nonsense mutation that abolishes synthesis of PrPC. In these animals, the PrP-encoding mRNA is rapidly degraded. Goats without...
A naturally occurring mutation in the PRNP gene of Norwegian dairy goats terminates synthesis of the cellular prion protein (PrPC), rendering homozygous goats (PRNPTer/Ter) devoid of the protein. Although PrPC has been extensively studied, particularly in the central nervous system, the biological role of PrPC remains incompletely understood. Here,...
Background:
Sepsis is a serious health problem associated with a range of infectious diseases in animals and humans. Early events of this syndrome can be mimicked by experimental administration of lipopolysaccharides (LPS). Compared with mice, small ruminants and humans are highly sensitive to LPS, making goats valuable in inflammatory models. We p...
Denne rapporten sammenstiller resultatet av en systematisk gjennomgang av vitenskapelig litteratur, innhenting av erfaringsbasert kunnskap og analyse av tilgjengelige felt- og forskningsdata om gjellelokkforkortelse og andre gjellelokkforandringer hos laksefisk. I tillegg omtales gjellelokkforandringer og årsaker og konsekvenser av disse hos andre...
Chronic wasting disease (CWD), a prion disease affecting cervids, has been known in North America (NA) since the 1960s and emerged in Norway in 2016. Surveillance and studies have revealed that there are different forms of CWD in Fennoscandia: contagious CWD in Norwegian reindeer and sporadic CWD in moose and red deer. Experimental studies have dem...
Background
Chronic wasting disease (CWD) is a prion disease of cervids first reported in North America in the 1960s. In Europe, CWD was first diagnosed in 2016 in a wild reindeer in Norway. Detection of two more cases in the same mountain area led to the complete culling of this partially confined reindeer population of about 2400 animals. A total...
Case-report av et utbrudd med Aeromonas veronii på laks i et RAS-anlegg. Rapporten beskriver anamnese, obduksjon og histologi, samt hvilke tiltak som ble satt inn.
Background: Chronic wasting disease (CWD) is a prion disease of cervids. In 2016, CWD was discovered for the first time in reindeer. The affected population was situated in Nordfjella mountain region in Norway. In an attempt to eradicate the disease, all reindeer in the region were culled during winter 2017-18. Because many sheep have their summer...
Varmtvannsbehandling har de siste årene blitt en av de vanligste formene for ikke-medikamentell avlusing på laksefisk. Metoden innebærer at laksen eksponeres for
oppvarmet sjøvann (29-34 °C) i en tidsperiode på 30-34 sekunder. Det har den siste tiden
kommet studier som peker mot at vanntemperatur over 28 °C kan være forbundet med
smerterelatert atf...
Prion diseases are progressive and fatal, neurodegenerative disorders described in humans and animals. According to the “protein-only” hypothesis, the normal host-encoded prion protein (PrPC) is converted into a pathological and infectious form (PrPSc) in these diseases. Transgenic knockout models have shown that PrPC is a prerequisite for the deve...
Studies in mice with ablation of Prnp, the gene that encodes the cellular prion protein (PrPC), have led to the hypothesis that PrPC is important for peripheral nerve myelin maintenance. Here, we have used a nontransgenic animal model to put this idea to the test; namely, goats that, due to a naturally occurring nonsense mutation, lack PrPC. Teased...
We utilized a unique line of Norwegian dairy goats that carry a stop mutation early in the PRNP to study PrPC physiology. These goats are the only known mammals that are naturally devoid of PrPC (PRNPTer/Ter). By using next generation RNA sequencing, we identified a primed state of interferon-stimulated genes in circulating blood cells and tissues...
Individual number of reads obtained from RNA sequencing.
Total reads, total mapped reads and uniquely mapped reads across all samples, n = 16, 8 of each genotype.
(TIF)
Differentially expressed genes between PRNPTer/Ter (n = 8) and PRNP+/+ (n = 8) goats (127 genes).
(DOCX)
Hierarchical clustering dendrogram.
Hierarchical clustering dendrogram of all genes after normalization of expression data (RPKM) using Euclidean distance and complete linkage.
(TIF)
Chromosomal distribution of differentially expressed genes.
(A) Frequency of differentially expressed genes (735 genes) per chromosome. Total number of genes per chromosome were obtained from National Center for Biotechnology Information (NCBI), based on the Capra hircus CHIR_1.0-Primary Assembly. (B) Chromosomal distribution of annotated different...
Clones of human neuroblastoma SH-SY5Y cells expressing human PRNP.
Protein expression of PrPC for untreated and PNGase-F-treated untransfected human neuroblastoma SH-SY5Y cells and SH-SY5Y clones transfected with human PRNP (n = 8), determined by Western Blot analysis using 6H4 mouse anti-PrPC as the primary antibody. Protein bands correspond to gl...
Forward and reverse primers used for qPCR.
(DOCX)
Differentially expressed genes (735 genes).
(XLSX)
Questions
Questions (5)
Hi. We have performed a small scale pilot study for the effect of an treatment (not for publication). The research items are classified as "postive" or "negative" for the disease based on different criteria (PCR, clinical examination, ++). Is there an appropriate test to see if the effect of antibiotics is statistically different from the control group?
Before treatment:
Group 1 (44/50 are positive)
Group 2 (48/50 are positive)
After treatment (antibiotics or placebo):
Group 1 - antibiotics (16/46 are positive)
Group 2 - saline control (33/50 are positive)
Thanks
Hi. How many animals do I need to test to detect at least one positive animal? (with 95 % confidence). Is it possible to estimate sample size based on the following variables?
Population: > 10000
Expected prevalence: about 1/3000 (0.033 %)
Diagnostic test: sensitivity 96 %, specificity 100 %.
Is there an online calculator for these type of calculations?
Bests, Øyvind
I am comparing temperature curves in two groups, n=8 in each group. I have measured fever at 29 time points after LPS stimuli, and want to test if one group respond different than the other. One group had a higher mean temperature at all time points during the study, but the difference was only significant at a few time points (multiple t-test, after confirming normality). Is there a appropriate statistical test for comparing the two temperature lines as a whole, and thereby get an overall p-value for a potential difference?
(I'm using GraphPad).
Hi.
I’m going to use Qiagen RNeasy Lipid Tissue Mini kit to extract RNA for RNAseq. Is additional (on column) DNase treatment necessary? Qiagen informs:
“Generally, DNase digestion is not required for RNA purified with RNeasy Kits since the silica-gel–membrane, spin-column technology efficiently removes the majority of the DNA without DNase treatment. However, more complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA. “
Is RNAseq classified as sensitive to very small amounts of DNA?
Thanks.
Øyvind.
I am using the PAXgene blood miRNA Kit (Qiagen) to isolate RNA from PAX blood tubes. The tubes were stored at -80 for 6 months, and are thawed for 3 hours before RNA isolation (allowing equilibration to room temperature). The first step in the protocol is centrifugation of the PAX blood tubes at 3000 g for 10 minutes. However, after adding RNAse free water I have trouble dissolve the pellet properly, which I suspect reduces the yield. I have tried to vortex for up to two minutes (e.g 6x20 seconds), but I still get relatively large pieces of pellet that wont dissolve. The same problem occurs after the centrifugation of step 4.
Does anyone have a solution to this?
