Othman E. Othman

Othman E. Othman
National Research Center, Egypt | NRC · Department of Cell Biology

Prof. of Molecular Genetics

About

99
Publications
17,244
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722
Citations
Additional affiliations
November 2006 - present
National Research Center, Egypt
Position
  • Prof. of Molecular Genetics and Animal Biotechnology
Description
  • Animal Biotechnology and Molecular Genetics: - Genetic polymorphism of QTL genes in farm animals using different techniques. - Genetic diversity between different breeds of farm animals using different techniques.
Education
October 1994 - October 1996
Cairo University
Field of study
  • Science (Genetics)

Publications

Publications (99)
Article
The objectives of the current study were to identify polymorphism in the prolactin receptor (PRLR) gene among three Egyptian goat breeds (Zaraibi, Damascus, and Barki) and to investigate the association between PRLR genotype, parity, season of kidding, and litter size factors with milk yield and reproductive traits of Zaraibi goats. One hundred and...
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Camels are considered an important food source in North Africa. Trypanosomiasis in camels is a life-threatening disease that causes severe economic losses in milk and meat production. Therefore, the objective of this study was to determine the trypanosome genotypes in the North African region. Trypanosome infection rates were determined by microsco...
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Background and Aim: Toll-like receptors (TLRs) are important for the recognition of pathogen-associated molecular patterns. Single nucleotide polymorphisms (SNPs) within TLRs have a potential impact on the alteration of susceptibility or resistance to inflammatory diseases. This work focused on a case-control study for the distribution of SNPs in T...
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Background Molecular genetics has been extremely useful in determining the relation between animal populations and documenting the degrees of genetic variation found within them. The present study was undertaken to evaluate genetic diversity and the relationships between the three camel populations reared in Egypt: Maghrabi, Sudani, and Baladi usin...
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(1) Background: It has been recognized that the origin of fat-tailed sheep occurred within coarse wool breeds and that this character was introgressed several times into thin-tailed populations. However, no study has investigated this idea for Egyptian breeds using mtDNA analyses. (2) Methods: Using new sequences of the control region, we construct...
Article
Background and Aim: Despite the economic importance of buffalo as a main source of milk and meat, only little attention has been directed to its immune and reproductive performance. The early diagnosis of subclinical endometritis may reduce the economic loss of buffalo’s production. The difference in expression profiles of immunity-related genes ha...
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Background and Aim: Uterine lumen contamination with bacteria is ubiquitous in buffalo after parturition. Nearly one-third of these infected animals develop endometritis which leads to reduced fertility. The present study aimed to evaluate the expressions of IFN-γ and NOS2 genes in uterine tissue of buffaloes with endometritis and comparing them wi...
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To understand the molecular and genetic mechanisms related to the litter size in one species of two different populations (high litter size and low litter size), we performed RNA-seq for the oocytes and granulosa cells (GCs) at different developmental stages of follicle, and identified the interaction of genes from both sides of follicle (oocyte an...
Article
Although some studies have investigated the DNA methylation modification in goat ovaries, it is not understood DNA methylation related to goat litter size. This investigation was designed to explore the DNA methylation status in the ovaries of high litter size and low litter size groups using whole-genome bisulfite sequencing (WGBS). We found that...
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The new technology of high throughput single cell RNA sequencing (10 × scRNA‐seq) was developed recently with many advantages. However, it was not commonly used in farm animal research. There are few reports for the gene expression of goat ovarian follicle granulosa cells (GCs) during different developmental stages. In current investigation, the ge...
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The objectives of this study were to identify polymorphisms in the lactoferrin gene among three Egyptian goat breeds (Barki, Zaraibi, and Damascus) and to investigate the effect of LF genotype, parity, and lactation stage on milk composition traits of Zaraibi goats. One hundred and thirty-two blood samples were collected for DNA extraction, with 53...
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The prion protein is coded by the PRP gene which is active in the brain and several other tissues. The functions of normal PrPs are related to energy production, protein degradation and DNA replication. Prions are the essential factor for the transmissible spongiform encephalopathies (TSEs) in different mammalian species. The present study aimed to...
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Bone morphogenetic protein 15 (BMP15/FecX) gene is considered one of the major genes and a candidate marker for the reproduction in farm animals, especially sheep. The present study aimed to detect the genetic polymorphisms of BMP15 gene in sheep using PCR-RFLP technique. In the present study, 115 ewes were assigned into high and low prolificacy ca...
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Foot and Mouth disease (FMD) is a contagious disease leads to economically loss in livestock production all over the world. This serious disease is caused due to the infection of the animal with a single-stranded RNA virus (FMDV). This study aimed to investigate the genetic polymorphism of BoLA-DRB3 gene in Egyptian buffalo as a candidate genetic m...
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One of the main sources of meat and milk in Egypt is buffalo, of river type. The marker assisted selection-depending on the promised genetic markers is considered to be the effective way for improvement of buffalo productivity. This work aimed to study the genetic structure and nucleotide sequences of three productivity genes namely; LGB, PIT-1, an...
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Aim: Cytochrome b (Cyt-b) regions of mtDNA have received more attention due to their role in the genetic diversity and phylogenetic studies in different livestock. By using Cytochrome b sequencing, we aimed to clarify the genetic affinities and phylogeny of six Egyptian sheep breeds. Methodology: The genomic DNA was extracted and the specific prime...
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Background: In January 2016, several cases of clinical signs and mortalities suggested foot and mouth disease (FMD) outbreaks among cattle and buffalo herds in Egypt. This study aimed to investigate the contribution of Foot and Mouth Disease Virus (FMDV) and characterization of the circulating strains associated with the outbreaks. Methods: The col...
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Horses are one of the early domesticated animals in the world that changed societies and civilizations on a continent-wide scale. Due to the rare information about the genetic characterization of different horse populations in Egypt, this study aimed to identify the genetic biodiversity and relationships between four horse populations reared in Egy...
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This study was aimed to assess cytochrome b conservation in six breeds of camels reared in Egypt and to compare its sequence with those of other livestock species. The 208-bp fragments from camel mtDNA cyto b were amplified using PCR for 54 camels belonging to 6 camel breeds reared in Egypt. The alignment of camel cyto b sequences showed the presen...
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More than 300 horse breeds or types are present all over the world and employed in different activities. This work aimed to identify genetic variations and SNPs in two effective mtDNA genes; ATP6 and ND2; among Arabian and Thoroughbred horses.The primer used in this study amplified 340-bp fragments from ATP6 gene. The results showed the presence of...
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The genetic improvement of production traits can be developed through marker assisted selection. Insulin-like growth factor I ( IGF -I) is a member of the somatotrophic axis which has a remarkable variation of its biological effect including protein synthesis and skeletal growth. This study aimed to detect the genetic polymorphism of IGF -1 in diff...
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Calpastatin is an nhibitor for calpain enzyme system and it has an essential role in meat quality and its tenderness. This trait is of great interest for meat industry and consumers. Molecular genetics techniques help in fishing out of molecular markers which affect the economically production traits in farm animals. Genetic polymorphism of CAST ge...
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HIS STUDY aimed to evaluate the sperm characteristics in buffalo characteristics in buffalo bulls and to screen the genetic genetic polymorphisms in PIT-1 gene as bases for selection of bulls with good breeding value. The study was performed on 60 buffalo bulls aged 2-8 years. The animals were divided into three groups according to the age. The fir...
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Ovulation can be regulated by a group of genes, termed as fecundity (Fec) genes. The aim of this study is to identify genetic polymorphism in the Booroola (FecB) gene in Barki, Rahmani, and Ossimi sheep breeds with different physiological status diagnosed by ultrasound. Accordingly, animals were early classified into three groups: carrying single f...
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Growth hormone (GH) gene has been described as a candidate gene for marker-assisted selection in different farm animals. The present study was designed to identify the polymorphism in GH gene and its association with variation of wool traits in Egyptian sheep breeds. Wool and blood samples were collected from 42 animals including two breeds (Barki...
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Camels play an important socioeconomic role within the pastoral and agricultural system in the dry and semidry zones of Asia and African where they are dual purpose animals (meat and milk). In spite of the effective role of casein genes with their polymorphisms on quantitative traits and technological properties of milk, the studies on genetic poly...
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Genetic information especially of the Quantitative Trait Loci (QTL) which affect different performance traits is considered one of the most effective tools in the breeding programs of livestock. Several genes were reported as candidate genes that effect litter size performance and one of these genes is the KISS1 which is considered as a regulator o...
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Tyrosinase is a key enzyme in the metabolic pathway leading to coat color pigmentation. Mutations in the tyrosinase (TYR) gene are responsible for the albino phenotype in mammals and chicken. Loss of tyrosinase mRNA expression prevents melanin synthesis, thereby causing albinism. The objective of this study was to detect the genetic variations and...
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Small ruminants are considered as one of the major sources of meat and milk production in Egypt. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of...
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A 721-bp fragment from 15,541 to 16,261 bp (NC_001941.1) of the mtDNA control region from different Egyptian and Italian sheep breeds was amplified. The PCR products were purified and sequenced. From the amplified fragment of 721-bp, a region of 423 bp after excluding a central region rich in tandem repeats was analyzed. Within all tested breeds, t...
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Authors' contributions This work was carried out in collaboration between all authors. Author OEO designed the study, followed up the practical work and wrote the final version of the manuscript. Author MFAS managed the analyses of the study, managed the literature searches and wrote the first draft of the manuscript. Author NAAEM performed the pra...
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Fibroblast growth factor 5 (FGF-5) gene is a member of the fibroblast growth factor (FGF) family. The protein encoded by this gene has a variety of biological activities including the embryonic development and cell growth. This study aimed to characterize FGF-5 gene and to detect the possible association between the polymorphism of this gene with b...
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Fibroblast growth factor 5 (FGF-5) gene is a member of the fibroblast growth factor (FGF) family. The protein encoded by this gene has a variety of biological activities including the embryonic development and cell growth. This study aimed to characterize FGF-5 gene and to detect the possible association between the polymorphism of this gene with b...
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Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene responsible for the multiple influences of insulin-like growth factors (IGFs) system. It is considered as a candidate gene for growth and production traits. In the present study, we aimed to determine the genetic polymorphism of Egyptian cattle IGFBP-3 gene. The amplif...
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Threats to biodiversity are increasing, whether measured in terms of extinction rate, destruction of ecosystems and habitat or loss of genetic diversity within the species utilized in agriculture. In these conditions, it is more strategically important than ever to preserve as much the farm animal diversity as possible, to ensure a prompt and prope...
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One of the physiological causes for true anestrum is a low level of ovarian estrogens. Estrogens are imperative for reproductive development, fertility, bone growth and sexual behavior. The key enzyme in estrogen biosynthesis is cytochrome P450 aromatase, the protein product of CYP19 gene. The genetic polymorphism of CYP19 gene might influence fert...
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Full-text available
Threats to biodiversity are increasing, whether measured in terms of extinction rate, destruction of ecosystems and habitat or loss of genetic diversity within the species utilized in agriculture. In these conditions, it is more strategically important than ever to preserve as much the farm animal diversity as possible, to ensure a prompt and prope...
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Full-text available
Sheep milk is an excellent raw material for the milk processing industry especially in cheese production. The protein content and composition of sheep milk are important in the cheese manufacturing. The casein fraction of ruminant milk proteins consists of four caseins, namely αs1, αs2, β and κ-Casein. Casein genetic polymorphisms are important due...
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Aim: The somatotropic axis (SA) comprises genes associated with economically important quantitative traits in livestock like mammary and muscle growth as well as carcass traits. Insulin growth factor-1 (IGF-1) and its receptor (IGF-1R) are two important genes belonging to the SA. The aim of this study was to evaluate the genetic polymorphism of IGF...
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Objective: Casein genetic polymorphisms are important and well known due to their effects on quantitative traits and technological properties of milk manufacturing. The casein fraction of ruminant milk proteins consists of four caseins, namely 8s1-, 8s2-, β-and K-casein. At the DNA level, polymerase chain reaction (PCR) and single-strand conformati...
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Objective: Casein genetic polymorphisms are important and well known due to their effects on quantitative traits and technological properties of milk manufacturing. The casein fraction of ruminant milk proteins consists of four caseins, namely 8s1-, 8s2-, 9-and K-casein. At the DNA level, polymerase chain reaction (PCR) and single-strand conformati...
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There is a considerable interest in the application of molecular genetics technologies in the form of specific DNA markers that are associated with various productivity traits to promote more efficient and relatively easy selection and breeding of farm animals with an advantage for inheritable traits of production and reproduction. River buffalo is...
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Full-text available
Objective: Casein genetic polymorphisms are important and well known due to their effects on quantitative traits and technological properties of milk manufacturing. The casein fraction of ruminant milk proteins consists of four caseins, namely αs1-, αs2-, β-and K-casein. At the DNA level, polymerase chain reaction (PCR) and single-strand conformati...
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Full-text available
The formulation of the modern breed concept during mid- 1800s has caused remarkable changes in the livestock sector: largescale production expanded [1] and its application to breeding and husbandry practices led to the formation of well-defined breeds, exposed to intense anthropogenic selection. As a consequence, farmers progressively substituted t...
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The domestic water buffalo is a species of great economic potential, especially in developing countries like Egypt. Egyptian buffalo have been classified according to minor phenotypic differences and their geographical locations. Few studies have taken place to investigate the genetic variations in Egyptian buffalo using microsatellites analysis. I...
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The major whey proteins, β-lactoglobulin (β-LG) and α-lactalbumin (α-LA), are strongly correlated with the nutritional value and the functional properties of milk whey. Ovine whey has a unique protein composition compared to that of bovine whey and it is especially rich in proteins. Genetic polymorphism of β-LG using PCR-RFLP and α-LA using PCR-SSC...
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Currently, the primary thrust of research in animal genetics is the identification of genes (so-called major genes), which affect the expression of quantitative traits markedly. Two of these major genes are growth hormone (GH) and its receptor (GHR) genes which are related to production and reproduction traits in livestock. There is extensive liter...
Data
The domestic water buffalo is a species of great economic potential, especially in developing countries like Egypt. Egyptian buffalo have been classified according to minor phenotypic differences and their geographical locations. Few studies have taken place to investigate the genetic variations in Egyptian buffalo using microsatellites analysis. I...
Article
Full-text available
In dairy farm animals, the principal goal of the selection is the improvement of milk yield and composition. The genes of milk proteins and hormones are excellent candidate genes for link-age analysis with quantitative trait loci (QTL) because of their biological significance on the quan-titative traits of interest. Prolactin (PRL) is a polypeptide...
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Leptin and l-calpain have been considered as two candidate genes for carcass performance and meat quality traits in the farm animals. The micromolar calcium-activated neutral protease (CAPN1) gene encodes l-calpain that degrades myofibril proteins under the postmortem conditions which appears to be the primary enzyme in the postmortem tenderization...
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The role of the BoLA-DRB3 gene in the development of an immune response system and its highly polymorphic nature make it one of the most important candidate genes in disease resistance studies. PCR-RFLP was used in this study to detect the genotypic polymorphism of BoLA-DRB3 exon 2 in 50 Egyptian buffalo. The PCR amplified fragments at 284-bp were...
Data
Full-text available
The role of the BoLA-DRB3 gene in the development of an immune response system and its highly polymorphic nature make it one of the most important candidate genes in disease resistance studies. PCR-RFLP was used in this study to detect the genotypic polymorphism of BoLA-DRB3 exon 2 in 50 Egyptian buffalo. The PCR amplified fragments at 284-bp were...
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Full-text available
The genes that encode the major protein and whey protein of milk are candidate genes in molecular marker assisted selection to improve the milk productivity in farm animals. Our study concerned with genotyping analysis of four milk genes, CSN1S1, CSN1S2, KCN and β-LG in four goat breed reared in Egypt; Baladi, Barki, Damascus and Zaraibi. Our resul...
Data
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Sheep is one of the most important domestic animals in Egypt like many other developing countries. It is raised mainly for meat production with carpet sheep wool as a secondary product. Using RAPD-PCR assay, the genetic variation among five Egyptian sheep breeds namely: Rahmani, Ossimi, Barki, Saidi and Sohagi were analyzed. Fifty-seven bands were...
Article
Full-text available
Sheep is one of the most important domestic animals in Egypt like many other developing countries. It is raised mainly for meat production with carpet sheep wool as a secondary product. Using RAPD-PCR assay, the genetic variation among five Egyptian sheep breeds namely: Rahmani, Ossimi, Barki, Saidi and Sohagi were analyzed. Fifty-seven bands were...
Article
Full-text available
Sheep is one of the most important domestic animals in Egypt like many other developing countries. It is raised mainly for meat production with carpet sheep wool as a secondary product. Using RAPD-PCR assay, the genetic variation among five Egyptian sheep breeds namely: Rahmani, Ossimi, Barki, Saidi and Sohagi were analyzed. Fifty-seven bands were...
Article
Full-text available
This study aimed to detect the genetic variants of kappa-casein (k-CN) in four breeds of goat reared in Egypt using PCR-RFLP technique. Three primers 13F, kb1 and kb2 with two restriction enzymes Alw44I and HaeIII have been used for genotyping k-CN exon 4. The PCR product -resulted from kb1 and kb2 primers- with size 459 bp was digested by Alw44I e...
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In this study, we used restriction endonuclease cleavage sites ( Hae III) as tools to study polymorphisms in buffalo exon 2 of DRB gene. Our results showed that the animals with homozygous restriction patterns are present in high percentage (90%) compared with heterozygous restriction patterns (10%). The homozygous restriction pattern at 190 bp ill...
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Forty-five animals belonging to four Egyptian goat breeds were undertaken in this study to analyze the different alleles of CSN1S2 gene. The PCR products of five different primers were digested by restriction enzymes Mse I, Pst I, Nco I, Nla III and Alw26 I for detecting the presence of seven alleles A and B, C, D and 0, E and F, respectively. Pres...
Data
Full-text available
In this study, we used restriction endonuclease cleavage sites (HaeIII) as tools to study polymorphisms in buffalo exon 2 of DRB gene. Our results showed that the animals with homozygous restriction patterns are present in high percentage (90%) compared with heterozygous restriction patterns (10%). The homozygous restriction pattern at 190 bp illus...
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Gene maps and genetic polymorphisms of genes related to the economic quantitative traits as tools for developing more efficient breeding strategies have as targeted animal improvement by the genomic approach. This study aimed to identify the genetic polymorphism and gene mapping of two genes, BTN and ODC, are associated with milk composition in riv...
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This study aimed to analysis the genetic polymorphisms of MHC class II DRB gene in the Egyptian goat using PCR-RFLP method. The amplified fragment with size of 285-bp was digested by two restriction enzymes Taq I and Pst I. Restriction digestion of PCR product by Taq I enzyme represented two digested fragments at 122- and 163-bp (T restriction patt...
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The present study demonstrated the cytogenetic and hematological effects of the antibiotic chloramphenicol in blood cultures of calves, at 4 days and 3 weeks after stoppage of injection with chloramphenicol by the minimal therapeutic dose (once a day for 4 successive days). The different types of chromosomal aberrations including breaks, gaps, dele...
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Caseins are a family of milk proteins that exist in several molecular forms (alpha s1, alpha s2, beta and kappa) with variant alleles of each. Kappa-casein exists commonly as A or B variants and are of particular importance to the quality of the milk. In this study, the PCR-RFLP method was used for differentiating buffalo k-CN alleles. This method...