Osman Ahmed

Osman Ahmed
Karolinska Institutet | KI · Department of Medicine, Solna

MD. MSc. PhD

About

31
Publications
4,828
Reads
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282
Citations
Introduction
By the end of 2019, I have started Novo Nordisk postdoctoral fellowship at Karolinska Institutet. The project; Neural control of inflammation and metabolism in liver is to investigate the lipid-loading effects on individual cells, how this can lead to pro and anti-inflammatory responses in the liver and how the cell types of the liver respond to the onset of NAFLD. Moreover, we will study how neural signals regulate inflammation in NAFLD. https://ki.se/en/srp-diabetes/novo-nordisk-fellowships
Additional affiliations
October 2013 - present
Karolinska Institutet
Position
  • PhD Student
Description
  • : research group interested in lipid metabolism and its role in cardio-metabolic disorders which I joined in November 2012 as research trainee with Professor Paolo Parini the head of the group at Division of Clinical Chemistry
November 2009 - November 2012
University of Khartoum
Position
  • Lecturer
Description
  • Conducting several biochemistry courses, giving lectures, participating in department meetings, examination preparation and correction, group discussions,tutorials for the students . Supervising teaching assistants and master students activities.
April 2007 - November 2009
University of Khartoum
Position
  • Research Assistant
Description
  • supervising undergraduate student activities (group discussions, laboratory work, seminars and tutorials), attending department meetings, assist in examination preparation and correction
Education
October 2013 - October 2017
Karolinska Institutet
Field of study
  • Clinical Chemistry
May 2007 - March 2009
University of Khartoum
Field of study
  • Medical Biochemistry
December 1999 - April 2006
University of Khartoum
Field of study
  • Medicine

Publications

Publications (31)
Article
Background Sterol O-Acyltransferase 2 (Soat2) encodes acyl-Coenzyme A:cholesterol acyltransferase 2 (ACAT2), which synthesizes cholesteryl esters in hepatocytes and enterocytes fated either to storage or to secretion into nascent triglyceride-rich lipoproteins. Objectives We aimed to unravel the molecular mechanisms leading to reduced hepatic stea...
Article
Introduction and Hypothesis: PCSK9-inhibition reduces the residual cardiovascular risk associated with LDL-cholesterol (LDL-C), but the wide-spread use of the antibody-based therapies is affected by the costs, especially in low- and middle-income countries. Hence, there is a need to identify affordable and effective drugs to inhibit PCSK9. Methods:...
Conference Paper
Aim: Recent reports show an association between statins therapy and reduction in 28-day all-cause mortality among Coronavirus disease 2019 (COVID-19) patients. This association is poorly understood mechanistically. Hence, we investigated whether simvastatin regulates hepatic expression of genes involved in humoral and innate immune responses, and i...
Chapter
The determination of the lipid content in liver offers the potential to investigate metabolic alterations in different research contexts. Here, we describe a method to determine cholesterol, triglycerides, and phospholipids in liver samples based on total lipid isolation by a 2:1 chloroform–methanol mixture (Folch extraction) and specific enzymatic...
Article
Full-text available
The response to overfeeding is sex dependent, and metabolic syndrome is more likely associated to obesity in men or postmenopausal women than in young fertile women. We hypothesized that obesity-induced metabolic syndrome is sex dependent due to a sex-specific regulation of the fatty acid (FA) synthesis pathways in liver and white adipose depots. W...
Thesis
Full-text available
The liver is the central organ for lipid, lipoprotein and glucose homeostasis, thus hepatic metabolic disturbances can predispose individuals to develop cardiometabolic disorders (CMD) such as atherosclerotic cardiovascular diseases (ASCVD), type 2 diabetes mellitus (T2DM), and nonalcoholic fatty liver disease (NAFLD).The overall aim of this thesis...
Article
Full-text available
Obesity triggers the development of non-alcoholic fatty liver disease (NAFLD), which involves alterations of regulatory transcription networks and epigenomes in hepatocytes. Here we demonstrate that G protein pathway suppressor 2 (GPS2), a subunit of the nuclear receptor corepressor (NCOR) and histone deacetylase 3 (HDAC3) complex, has a central ro...
Article
Full-text available
Background In randomized trials (SHARP [Study of Heart and Renal Protection], IMPROVE‐IT [Improved Reduction of Outcomes: Vytorin Efficacy International Trial]), combination of statin and ezetimibe resulted in additional reduction of cardiovascular events. The reduction was greater in patients with type 2 diabetes mellitus (T2DM), where elevated re...
Data
Data S1. Supplemental methods. Table S1. Lipid Contribution in Lipoprotein Fractions Used in Figure 2 Table S2. Contribution of Different Subclasses for Their Respective Fraction Baseline Table S3. Correlation of Percentage Changes of Proteoglycan (PG) Binding and Lipoprotein Particle Number and Composition Table S4. Safety Assessment Analyses
Article
Depletion of the cholesterol esterifying enzyme acyl-Coenzyme A: cholesterol acyltransferase 2 (ACAT2, encoded by Soat2) protects mice from atherosclerosis, diet-induced hypercholesterolemia, and hepatic steatosis when fed high-cholesterol diet. The glucose transporter 2 (GLUT2) represents the main gate of glucose uptake by the liver. Lipid synthes...
Article
Simvastatin and ezetimibe reduce plasma lipoprotein binding to human arterial proteoglycans in gallstone diseased patients. Ahmed O1,2#, Littmann K1#, Gustafsson U3, Pramfalk C1,4, Öörni K5, Larsson L1, Sahlin S3, Camejo G1, Parini P1,4,6, Eriksson M6 1 Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet, Sto...
Article
Background and aims: Transforming growth factor β induced factor homeobox 1 (TGIF1) is a transcriptional repressor that limits the response to transforming growth factor ß signaling and also represses transcription independent of this pathway. Recently, we found higher serum cholesterol levels and more hepatic lipid accumulation in mice lacking Tg...
Conference Paper
Introduction: Large genetic studies have recently recognized remnant-cholesterol, defined as cholesterol in triglyceride (TG)-rich lipoproteins, as a causal risk factor for ischemic heart disease and low grade inflammation, independently of HDL-cholesterol. NPC1L1 in the intestine is the target of ezetimibe, but its function in the liver is still u...
Article
Full-text available
Background Visceral leishmaniasis (VL), caused by protozoa of the Leishmania donovani complex, is a widespread parasitic disease of great public health importance; without effective chemotherapy symptomatic VL is usually fatal. Distinction of asymptomatic carriage from progressive disease and the prediction of relapse following treatment are hamper...
Article
Full-text available
Background: Visceral leishmaniasis (VL), a widely distributed systemic disease caused by infection with the Leishmania donovani complex (L. donovani and L. infantum), is almost always fatal if symptomatic and untreated. A rapid point-of-care diagnostic test for anti-Leishmania antibodies, the rK39-immunochromatographic test (rK39-ICT), has high se...
Article
Full-text available
The liver X receptors (LXR)α and LXRβ are transcription factors belonging to the nuclear receptor family which play a central role in metabolic homeostasis being master regulators of key target genes in the glucose and lipid pathways. Wild-type (Wt), LXRα(-/-) and LXRβ(-/-) mice were fed a chow diet with (treated) or without (control) the synthetic...
Article
Full-text available
Background. Poor access to diagnosis stymies control of visceral leishmaniasis (VL). Antibody-detecting rapid diagnostic tests (RDTs) can be performed in peripheral health settings. However, there are many brands available and published reports of variable accuracy. Methods. Commercial VL RDTs containing bound rK39 or rKE16 antigen were evaluated...
Data
Full-text available
STARD Checklist (0.13 MB PDF)
Article
Full-text available
The Leishmania OligoC-TesT and NASBA-Oligochromatography (OC) were recently developed for simplified and standardised molecular detection of Leishmania parasites in clinical specimens. We here present the phase II evaluation of both tests for diagnosis of visceral leishmaniasis (VL), cutaneous leishmaniasis (CL) and post kala-azar dermal leishmania...

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Projects

Projects (2)
Project
In this clinical trial with trnscriptomic approach we aimed to characterize the effects of the combination of simvastatin and ezetimibe in human liver to uncover the different effects on hepatic lipid & carbohydrate metabolic pathways.
Project
In this clinical trial we evaluate the effect of simvastatin+/-ezetimibe on biliary, hepatic and lipoprotein lipid metabolism. In addition to their effect on binding arterial proteoglycan