Oskar Staufer

Oskar Staufer
Max Planck Institute for Medical Research | MPIMF · Department of Cellular Biophysics

Doctor of Philosophy

About

37
Publications
3,507
Reads
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442
Citations
Additional affiliations
January 2020 - July 2020
MP Center for Minimal Biology Bristol
Position
  • Research Associate
September 2018 - July 2020
University Hospital Heidelberg
Position
  • Lecturer
November 2017 - May 2020
Max Planck Institute for Medical Research
Position
  • PhD Student
Education
September 2011 - May 2017
Heidelberg University
Field of study
  • Molecular Biotechnology

Publications

Publications (37)
Article
Full-text available
Development of regulated cellular processes and signaling methods in synthetic cells is essential for their integration with living materials. Light is an attractive tool to achieve this, but the limited penetration depth into tissue of visible light restricts its usability for in-vivo applications. Here, we describe the design and implementation o...
Article
Immune vigilance ensures body integrity by eliminating malignant cells through the complex but coordinated cooperation of highly diversified lymphocytes populations. The sheer complexity of the immune system has slowed development of immunotherapies based on top-down genetic engineering of lymphocytes. In contrast, bottom-up assembly of synthetic c...
Article
Full-text available
Extracellular vesicles (EVs) are fundamental for proper physiological functioning of multicellular organisms. By shuttling nucleic acids and proteins between cells, EVs regulate a plethora of cellular processes, especially those involved in immune signalling. However, the mechanistic understanding concerning the biophysical principles underlying EV...
Article
Full-text available
SARS-CoV-2 infection is a major global public health concern with incompletely understood pathogenesis. The SARS-CoV-2 spike (S) glycoprotein comprises a highly conserved free fatty acid binding pocket (FABP) with unknown function and evolutionary selection advantage1,2. Deciphering FABP impact on COVID-19 progression is challenged by the heterogen...
Article
Full-text available
As the global burden of SARS-CoV-2 infections escalates, so does the evolution of viral variants with increased transmissibility and pathology. In addition to this entrenched diversity, RNA viruses can also display genetic diversity within single infected hosts with co-existing viral variants evolving differently in distinct cell types. The BriSΔ v...
Article
Full-text available
Employing concepts from physics, chemistry and bioengineering, 'learning-by-building' approaches are becoming increasingly popular in the life sciences, especially with researchers who are attempting to engineer cellular life from scratch. The SynCell2020/21 conference brought together researchers from different disciplines to highlight progress in...
Preprint
Empowered by emerging concepts from physics, chemistry, and bioengineering, learning-by-building approaches have found increasing application in the life sciences. Particularly, they are directed to tackle the overarching goal of engineering cellular life from scratch. The SynCell2020/21 conference brought together a diverse group of researchers to...
Article
Full-text available
Extracellular vesicles (EVs) are fundamental for intercellular communication and influence nearly every process in cell physiology. However, because of their intricate molecular complexity, quantitative knowledge on their signaling mechanisms is missing, particularly impeding their therapeutic application. We used a complementary and quantitative e...
Preprint
Full-text available
Development of regulated cellular processes and signaling methods in synthetic cells is essential for their integration with living materials. Light is an attractive tool to achieve this, but the limited penetration depth into tissue of visible light restricts its usability for in-vivo applications. Here, we describe the synthesis and application o...
Preprint
As the global burden of SARS-CoV-2 infections escalates, so does the evolution of viral variants which is of particular concern due to their potential for increased transmissibility and pathology. In addition to this entrenched variant diversity in circulation, RNA viruses can also display genetic diversity within single infected hosts with co-exis...
Article
Full-text available
COVID-19, caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), represents a global crisis. Key to SARS-CoV-2 therapeutic development is unraveling the mechanisms driving high infectivity, broad tissue tropism and severe pathology. Our 2.85 Å cryo-EM structure of SARS-CoV-2 spike (S) glycoprotein reveals that the receptor binding...
Article
Full-text available
Lipid-based vesicles have found widespread applications in the life sciences, allowing for fundamental insights into membrane-based processes in cell biology and as carrier systems for drug delivery purposes. So far, mostly small unilamellar vesicles (SUVs) with diameters of ~100 nm have been applied as carrier systems for biomedical applications....
Article
Full-text available
Creating a magic bullet that can selectively kill cancer cells while sparing nearby healthy cells remains one of the most ambitious objectives in pharmacology. Nanomedicine, which relies on the use of nanotechnologies to fight disease, was envisaged to fulfill this coveted goal. Despite substantial progress, the structural complexity of therapeutic...
Article
This study reports on the development of vertical, partially encapsulated nanoelectrodes for electrically contacting the interior of electrogenic cells with microelectronics. Intracellular electrical stimulation and recording with single cell resolution enables new insights into the electrophysiology of cells embedded in a complex multicellular net...
Preprint
Full-text available
COVID-19, caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), represents a global crisis. Key to SARS-CoV-2 therapeutic development is unraveling the mechanisms driving high infectivity, broad tissue tropism and severe pathology. Our cryo-EM structure of SARS-CoV-2 spike (S) glycoprotein reveals that the receptor binding domains...
Article
Full-text available
Exposure to live bacteria and accumulation of dead bacteria during bactericidal processes can cause bacterial infectious diseases, implant failure, and antibacterial surface deterioration. Microcapsules with asymmetrically distributed, funnel‐shaped pores, which are capable of capturing, retaining, and killing bacteria are developed, offering a sol...
Article
Full-text available
Bottom-up synthetic biology has directed most efforts toward the construction of artificial compartmentalized systems that recreate living cell functions in their mechanical, morphological, or metabolic characteristics. However, bottom-up synthetic biology also offers great potential to study subcellular structures like organelles. Because of their...
Article
Full-text available
Here, we introduce a one-pot method for the bottom-up assembly of complex single- and multi-compartment synthetic cells. Cellular components are enclosed by giant unilamellar vesicles (GUVs), produced - at milliliter scale - directly from small unilamellar vesicles (SUVs) or proteoliposomes with only basic laboratory equipment within minutes. Towar...
Article
Full-text available
Coordinated collective electrochemical signals in multicellular assemblies, such as ion fluxes, membrane potentials, electrical gradients and steady electric fields, play an important role in cell and tissue spatial organization during many physiological processes like wound healing, inflammatory responses and hormone release. This mass of electric...
Article
Full-text available
Cell biology considers most animal tissues as assemblies of "individual" cells that rely on different contact-dependent communication mechanisms, including synapses, gap junctions or - a recent awareness - membrane nano- and microtubes. However, by protease-mediated singularization of dense 2D/ 3D cell cultures and tissue explants, we show here tha...
Article
Full-text available
Increased pro-inflammatory signaling is a hallmark of metabolic dysfunction in obesity and diabetes. Although both inflammatory and energy substrate handling processes represent critical layers of metabolic control, their molecular integration sites remain largely unknown. Here, we identify the heterodimerization interface between the α and β subun...
Article
Full-text available
The functional fusion of "living" biomaterial (such as cells) with synthetic systems has developed into a principal ambition for various scientific disciplines. In particular, emerging fields such as bionics and nanomedicine integrate advanced nanomaterials with biomolecules, cells and organisms in order to develop novel strategies for applications...
Chapter
Man kann keine wissenschaftliche Arbeit veröffentlichen, wenn man seine Messungen nicht korrekt darstellt oder durchgeführte Studien falsch auswertet. Als erfolgreicher Wissenschaftler sollte man mit beiden Beinen fest auf stati(sti)schem Boden stehen.
Chapter
Dieses Kapitel soll die wesentlichen Grundlagen linearer Gleichungssysteme erklären. Lineare Gleichungen sind von besonders einfacher Struktur und deswegen leicht zu handhaben. Außerdem sind sie für praktische Belange unerlässlich. Komplizierte Systeme können oft durch eine Reduktion auf lineare Probleme effizient gelöst werden. Bei dem Versuch, al...
Chapter
Wir haben bereits in Kap. 1 gesehen, dass Funktionen eine wichtige Rolle bei der Beschreibung biologischer Prozesse spielen. Die Exponentialfunktion ist z. B. geeignet, das Wachstum von Escherichia coli zu beschreiben. Die Gompertz-Funktion charakterisiert die Expansion eines Tumors. Ebenso gibt es Funktionen für die Veränderung der Konzentrationen...
Chapter
Die schließende Statistik wird benötigt, um von vereinzelten Messwerten (Stichproben) auf das große Ganze (die Grundgesamtheit) zu schließen.
Chapter
Will man Messungen miteinander in Beziehung setzen, bieten sich Funktionen an. Mit ihnen kann man beispielsweise Proportionen oder Abhängigkeitsverhältnisse zwischen Ergebnissen darstellen. Wir werden zunächst erklären, was Funktionen sind und wie man mit ihnen umgeht. Dann werden wir uns ein paar klassische Bausteine in Form von mathematisch relev...
Chapter
Wie wahrscheinlich ist es, dass ich Mathe jemals wieder brauche? “, fragt sich Dr. Arnold, der Otto Normalbiologe.

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